LOCUS       sequence-292254-        9088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Light-controlled tri-directional targeting of endogenous proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9088)
  AUTHORS   Redchuk TA, Karasev MM, Verkhusha PV, Donnelly SK, Hulsemann M, 
            Virtanen J, Moore HM, Vartiainen MK, Hodgson L, Verkhusha VV
  TITLE     Optogenetic regulation of endogenous proteins.
  JOURNAL   Nat Commun. 2020 Jan 30;11(1):605. doi: 10.1038/s41467-020-14460-4.
  PUBMED    32001718
REFERENCE   2  (bases 1 to 9088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-01-30"; volume: "11(1):605. doi"; pages: " 
            10.1038/s41467-020-14460-4"
COMMENT     Sequence Label: pQP-iRISB
FEATURES             Location/Qualifiers
     source          1..9088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        122..425
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        426..629
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     580..600
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      677..686
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2971..3027
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    3029..3603
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3053..3605
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3197..3214)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3424..3443
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3698..4039
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid 
                     antibody (Rothbauer et al., 2008)"
                     /label=GFP nanobody
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
     CDS             4061..4768
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4206..4224)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4474..4493
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             5273..5287
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     polyA_signal    5937..6058
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5974..5993)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6028..6047
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6065..6520)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6202..6223)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6414..6433
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6547..6651
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6653..7010
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6653..6844
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6676..6696)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6861..6996
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7045..7839
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7045..7839
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7099..7118)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7709..7728
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8027..8046)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8071..8118
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8447..9035
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8936..8955
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca
       61 ttagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
      121 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
      181 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      241 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      301 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      361 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      421 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
      481 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
      541 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
      601 tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgct
      661 accggactca gatctaccat ggtggcaggt catgcctctg gcagccccgc attcgggacc
      721 gccgatcttt cgaattgcga acgtgaagag atccacctcg ccggctcgat ccagccgcat
      781 ggcgcgcttc tggtcgtcag cgagccggat catcgcatca tccaggccag cgccaacgcc
      841 gcggaatttc tgaatctcgg aagcgtgctc ggcgttccgc tcgccgagat cgacggcgat
      901 ctgttgatca agatcctgcc gcatctcgat cccaccgccg aaggcatgcc ggtcgcggtg
      961 cgctgccgga tcggcaatcc ctccacggag tacgacggtc tgatgcatcg gcctccggaa
     1021 ggcgggctga tcatcgagct cgaacgtgcc ggcccgccga tcgatctgtc cggcacgctg
     1081 gcgccggcgc tggagcggat ccgcacggcg ggctcgctgc gcgcgctgtg cgatgacacc
     1141 gcgctgctgt ttcagcagtg caccggctac gaccgggtga tggtgtatcg cttcgacgag
     1201 cagggccacg gcgaagtgtt ctccgagcgc cacgtgcccg ggctcgaatc ctatttcggc
     1261 aaccgctatc cgtcgtcgga cattccgcag atggcgcggc ggctgtacga gcggcagcgc
     1321 gtccgcgtgc tggtcgacgt cagctatcag ccggtgccgc tggagccgcg gctgtcgccg
     1381 ctgaccgggc gcgatctcga catgtcgggc tgcttcctgc gctcgatgtc gccgatccat
     1441 ctgcagtacc tgaagaacat gggcgtgcgc gccaccctgg tggtgtcgct ggtggtcggc
     1501 ggcaagctgt ggggcctggt tgcctgtcac cattatctgc cgcgcttcat ccatttcgag
     1561 ctgcgggcga tctgcgaact gctcgccgaa gcgatcgcga cgcggatcac cgcgcttgag
     1621 agcttcgcgc agagccagtc ggagctgttc gtgcagcggc tcgaacagcg catgatcgaa
     1681 gcgatcaccc gtgaaggcga ttggcgcgca gcgattttcg acaccagcca atcgatcctg
     1741 cagccgctgc acgccgacgg ttgcgcgctg gtgtacgaag accagatcag gaccatcggt
     1801 gacgtacctt ccacgcagga tgttcgcgag atcgccgggt ggctcgatcg ccagccacgt
     1861 gcggcggtga cctcgaccgc gtcgctcggt ctcgacgtgc cggagctcgc gcatctgacg
     1921 cggatggcga gcggcgtggt cgcggcgccg atttcggatc atcgcggcga gtttctgatg
     1981 tggttccgcc ccgagcgcgt ccacaccgtt acctggggcg gcgatccgaa gaagccgttc
     2041 acgatgggcg atacaccggc ggatctgtcg ccgcggcgct ccttcgccaa atggcatcag
     2101 gttgtcgaag gcacgtccga tccgtggacg gccgccgatc tcgccgcggc tcgcaccatc
     2161 ggtcagaccg tcgccgacat cgtgctgcaa ttccgcgcgg tgcggacact gatcgcccgc
     2221 gaacagtacg aacagttttc gtcccaggtg cacgcttcga tgcagccggt gctgatcacc
     2281 gacgccgaag gccgcatcct gctgatgaac gactcgttcc gcgacatgtt gccggcgggg
     2341 tcgccatccg ccgtccatct cgacgatctc gccgggttct tcgtcgaatc gaacgatttc
     2401 ctgcgcaacg tcgccgaact gatcgatcac ggccgcgggt ggcgcggcga agttctgctg
     2461 cgcggcgcag gtaatcgccc gttgccgctg gcagtgcgcg ccgatccggt gacgcgcacg
     2521 gaggaccagt cgctcggctt cgtgctgatc ttcagcgacg ctaccgatcg tcgcaccgca
     2581 gatgccgcac gcacgcgttt ccaggaaggc attcttgcca gcgcacgtcc cggcgtgcgg
     2641 ctcgactcca agtccgacct cttgcacgag aagctgctgt ccgcgctggt cgagaacgcg
     2701 cagcttgccg cattggaaat tacttacggc gtcgagaccg gacgcatcgc cgagctgctc
     2761 gaaggcgttc gccagtcgat gctgcgcacc gccgaagtgc tcggccatct ggtgcagcac
     2821 gcggcgcgca cggccggcag cgacagctcg agcaatggct cgcagaacaa gaaggctagc
     2881 agtgctggcg gtagtgctgg tagtgctggt cgtacggcca gcggtaaaaa gaagaaaaag
     2941 aagtcaaaga caaagtgtgt aattatgtaa agatctcgag ctcaagcttc gaattctgca
     3001 gtcgacggta ccgcgggccc gggatccgcc cctctccctc cccccccccc taacgttact
     3061 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     3121 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     3181 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     3241 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     3301 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     3361 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     3421 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     3481 tgtatgggat ctgatctggg gcctcggtac acatgcttta catgtgttta gtcgaggtta
     3541 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     3601 taatatggcc acaaaccggt ccatgggaat tgatctctca ggattaacac tgcagagcgg
     3661 cggagggagc ggcggatccg gagggggctc tggccaagtc caactggtgg agtctggtgg
     3721 cgctttggtg cagccaggtg gctctctgcg tttgtcctgt gccgcttctg gcttcccagt
     3781 gaaccgctat tccatgcgct ggtatcgcca ggctccaggc aaagagcgtg agtgggtagc
     3841 cggtatgtcc agcgcgggtg atcgtagctc ctatgaagac tccgtgaagg gccgtttcac
     3901 catcagccgt gacgatgccc gtaacacggt gtatctgcaa atgaacagct tgaaacctga
     3961 agatacggcc gtgtattact gtaatgtgaa cgtgggcttc gagtattggg gccaaggcac
     4021 ccaggtcacc gtctccagcg gaggcggatc atctactagt atggtgagca agggcgagga
     4081 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
     4141 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     4201 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
     4261 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
     4321 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     4381 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
     4441 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
     4501 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     4561 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
     4621 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
     4681 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
     4741 caccggcggc atggacgagc tgtacaagag tgctggtggg tccgccggcg ggagtgctgg
     4801 cggtagtgcc ggcggtagtt ctagaggcaa gaacatgcag gcggtcaccg agctgcattc
     4861 ccggctgatc gctgcgcagc aggcgatgga gcgcgactat tggcggttgc gtgaattgga
     4921 gactcgctac cgcctggtgt tcgacgctgc cgccgatgcg gtgatgatcg tctccgccgg
     4981 cgacatgcgc atcgtcgaag ccaaccgggc ggcggtgaat gcgatcagcc gcgtcgagcg
     5041 cggcaatgac gaccttgcgg ggcgtgattt cctcgccgaa gtggcggctg ccgatcgcga
     5101 tgcggtgcgc gacatgctgg cccaggtgcg tcagcgcggc accgcactca gcgtcctcgt
     5161 tcatctcggc cgttacgacc gcgcctggat gctgcgcggt tcgctgatgt cgtccgagcg
     5221 tcgtcaggtt ttcctgctgc acttcacccc ggtgaccacg actcccgcga tcgacgacga
     5281 cgacaaaggt gtcgttgctt ctgctgccga cggtgcagag ggcgcctcag acgacgcaga
     5341 ggatgaattc ccggctagcg gcgccggcgc ctttttggct actacacttg aacgtattga
     5401 gaagaacttt gtcattactg acccaaggtt gccagataat cccattatat tcgcgtccga
     5461 tagtttcttg cagttgacag aatatagccg tgaagaaatt ttgggaagaa actgcaggtt
     5521 tctacaaggt cctgaaactg atcgcgcgac agtgagaaaa attagagatg ccatagataa
     5581 ccaaacagag gtcactgttc agctgattaa ttatacaaag agtggtaaaa agttctggaa
     5641 cctctttcac ttgcagccta tgcgagatca gaagggagat gtccagtact ttattggggt
     5701 tcagttggat ggaactgagc atgtccgaga tgctgccgag agagagggcg ttatgctggc
     5761 caagaagacc gccgagaaca tcgacatggc tgccaagagg gtcaagttgg actgagcggc
     5821 cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     5881 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     5941 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6001 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag
     6061 gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     6121 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     6181 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     6241 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     6301 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     6361 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     6421 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     6481 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
     6541 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     6601 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg
     6661 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     6721 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     6781 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     6841 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     6901 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     6961 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa
     7021 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     7081 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     7141 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     7201 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
     7261 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     7321 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     7381 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     7441 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     7501 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     7561 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     7621 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     7681 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     7741 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     7801 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     7861 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     7921 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     7981 gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg
     8041 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
     8101 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata
     8161 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc
     8221 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca
     8281 tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     8341 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     8401 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     8461 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     8521 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     8581 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     8641 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     8701 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     8761 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8821 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8881 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8941 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     9001 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     9061 ggttcctggc cttttgctgg ccttttgc
//
