LOCUS       sequence-292262-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-termial APEX2 tag. Gateway destination vector for mammanlian 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Jia J, Abudu YP, Claude-Taupin A, Gu Y, Kumar S, Choi SW, Peters R, 
            Mudd MH, Allers L, Salemi M, Phinney B, Johansen T, Deretic V
  TITLE     Galectins Control mTOR in Response to Endomembrane Damage.
  JOURNAL   Mol Cell. 2018 Apr 5;70(1):120-135.e8. doi: 
            10.1016/j.molcel.2018.03.009.
  PUBMED    29625033
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.03"; journalName: "Mol Cell"; date: 
            "2018-04-5- 5"; volume: "70"; issue: "1"; pages: "120-135.e8"
COMMENT     Sequence Label: pJJiaDEST-APEX2
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..773
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAXAHQKLSXLGVC*C"
     CDS             780..806
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     protein_bind    819..843
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tggnntannn gntgacgacg ataagggaaa gtcttaccca actgtgagtg ctgattacca
       61 ggacgccgtt gagaaggcga agaagaagct cagaggcttc atcgctgaga agagatgcgc
      121 tcctctaatg ctccgtttgg cattccactc tgctggaacc tttgacaagg gcacgaagac
      181 cggtggaccc ttcggaacca tcaagcaccc tgccgaactg gctcacagcg ctaacaacgg
      241 tcttgacatc gctgttaggc ttttggagcc actcaaggcg gagttcccta ttttgagcta
      301 cgccgatttc taccagttgg ctggcgttgt tgccgttgag gtcacgggtg gacctaaggt
      361 tccattccac cctggaagag aggacaagcc tgagccacca ccagagggtc gcttgcccga
      421 tcccactaag ggttctgacc atttgagaga tgtgtttggc aaagctatgg ggcttactga
      481 ccaagatatc gttgctctat ctgggggtca cactattgga gctgcacaca aggagcgttc
      541 tggatttgag ggtccctgga cctctaatcc tcttattttc gacaactcat acttcacgga
      601 gttgttgagt ggtgagaagg aaggtctcct tcagctacct tctgacaagg ctcttttgtc
      661 tgaccctgta ttccgccctc tcgttgacaa atatgcagcg gacgaagatg ccttctttgc
      721 tgattacgct gangctcacc aaaagctttc cnagcttggg gtttgctgat gccctgcagc
      781 tgcctcccct ngagcgcctg accctggacc tcgaatcaac aagtttgtac aaaaaagctg
      841 aacgagaaac gtaaaatgat ataaatatca ntatattaaa ttagattttg cntnaaaaaa
      901 cnnnntacat aatactg
//
