LOCUS       sequence-292280-        7481 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein 
            (Spike protein).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7481)
  TITLE     Truncated SARS-CoV-2 Spike protein constructs for making VSV pseudo 
            virus
REFERENCE   2  (bases 1 to 7481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4137386
FEATURES             Location/Qualifiers
     source          1..7481
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        136..515
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        516..719
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     670..690
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(4634..4653)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4699..4754
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4753..4772)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5115..5131)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5115..5131)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5128..5150)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5139..5155
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5163..5169,5170..5187,5188..5193))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5208..5229
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5288..5483
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5334..5469
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5489..5623
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5526..5545)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5580..5599
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5691..5708)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5862..6450)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5942..5961)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6621..7412,7413..7481))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7244..7263
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct gggtcgacat tgattattga ctagttatta atagtaatca attacggggt
      181 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      241 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      301 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      361 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      421 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      481 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
      541 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
      601 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
      661 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg
      721 gtttagtgaa ccgtcagatc agatctttgt cgatcctacc atccactcga cacacccgcc
      781 agcggccgcc accatgtttg tttttcttgt tttattgcca ctagtctcta gtcagtgtgt
      841 taatcttaca accagaactc aattaccccc tgcatacact aattctttca cacgtggtgt
      901 ttattaccct gacaaagttt tcagatcctc agttttacat tcaactcagg acttgttctt
      961 acctttcttt tccaatgtta cttggttcca tgctatacat gtctctggga ccaatggtac
     1021 taagaggttt gataaccctg tcctaccatt taatgatggt gtttattttg cttccactga
     1081 gaagtctaac ataataagag gctggatttt tggtactact ttagattcga agacccagtc
     1141 cctacttatt gttaataacg ctactaatgt tgttattaaa gtctgtgaat ttcaattttg
     1201 taatgatcca tttttgggtg tttattacca caaaaacaac aaaagttgga tggaaagtga
     1261 gttcagagtt tattctagtg cgaataattg cacttttgaa tatgtctctc agccttttct
     1321 tatggacctt gaaggaaaac agggtaattt caaaaatctt agggaatttg tgtttaagaa
     1381 tattgatggt tattttaaaa tatattctaa gcacacgcct attaatttag tgcgtgatct
     1441 ccctcagggt ttttcggctt tagaaccatt ggtagatttg ccaataggta ttaacatcac
     1501 taggtttcaa actttacttg ctttacatag aagttatttg actcctggtg attcttcttc
     1561 aggttggaca gctggtgctg cagcttatta tgtgggttat cttcaaccta ggacttttct
     1621 attaaaatat aatgaaaatg gaaccattac agatgctgta gactgtgcac ttgaccctct
     1681 ctcagaaaca aagtgtacgt tgaaatcctt cactgtagaa aaaggaatct atcaaacttc
     1741 taactttaga gtccaaccaa cagaatctat tgttagattt cctaatatta caaacttgtg
     1801 cccttttggt gaagttttta acgccaccag atttgcatct gtttatgctt ggaacaggaa
     1861 gagaatcagc aactgtgttg ctgattattc tgtcctatat aattccgcat cattttccac
     1921 ttttaagtgt tatggagtgt ctcctactaa attaaatgat ctctgcttta ctaatgtcta
     1981 tgcagattca tttgtaatta gaggtgatga agtcagacaa atcgctccag ggcaaactgg
     2041 aaagattgct gattataatt ataaattacc agatgatttt acaggctgcg ttatagcttg
     2101 gaattctaac aatcttgatt ctaaggttgg tggtaattat aattacctgt atagattgtt
     2161 taggaagtct aatctcaaac cttttgagag agatatttca actgaaatct atcaggccgg
     2221 tagcacacct tgtaatggtg ttgaaggttt taattgttac tttcctttac aatcatatgg
     2281 tttccaaccc actaatggtg ttggttacca accatacaga gtagtagtac tttcttttga
     2341 acttctacat gcaccagcaa ctgtttgtgg acctaaaaag tctactaatt tggttaaaaa
     2401 caaatgtgtc aatttcaact tcaatggttt aacaggcaca ggtgttctta ctgagtctaa
     2461 caaaaagttt ctgcctttcc aacaatttgg cagagacatt gctgacacta ctgatgctgt
     2521 ccgtgatcca cagacacttg agattcttga cattacacca tgttcttttg gtggtgtcag
     2581 tgttataaca ccaggaacaa atacttctaa ccaggttgct gttctttatc aggatgttaa
     2641 ctgcacagaa gtccctgttg ctattcatgc agatcaactt actcctactt ggcgtgttta
     2701 ttctacaggt tctaatgttt ttcaaacacg tgcaggctgt ttaatagggg ctgaacatgt
     2761 caacaactca tatgagtgtg acatacccat tggtgcaggt atatgcgcta gttatcagac
     2821 tcagactaat tctcctcggc gggcacgtag tgtagctagt caatccatca ttgcctacac
     2881 tatgtcactt ggtgcagaaa attcagttgc ttactctaat aactctattg ccatacccac
     2941 aaattttact attagtgtta ccacagaaat tctaccagtg tctatgacca agacatcagt
     3001 agattgtaca atgtacattt gtggtgattc aactgaatgc agcaatcttt tgttgcaata
     3061 tggcagtttt tgtacacaat taaaccgtgc tttaactgga atagctgttg aacaagacaa
     3121 aaacacccaa gaagtttttg cacaagtcaa acaaatttac aaaacaccac caattaaaga
     3181 ttttggtggt tttaattttt cacaaatatt accagatcca tcaaaaccaa gcaagaggtc
     3241 atttattgaa gatctacttt tcaacaaagt gacacttgca gatgctggct tcatcaaaca
     3301 atatggtgat tgccttggtg atattgctgc tagagacctc atttgtgcac aaaagtttaa
     3361 cggccttact gttttgccac ctttgctcac agatgaaatg attgctcaat acacttctgc
     3421 actgttagcg ggtacaatca cttctggttg gacctttggt gcaggtgctg cattacaaat
     3481 accatttgct atgcaaatgg cttataggtt taatggtatt ggagttacac agaatgttct
     3541 ctatgagaac caaaaattga ttgccaacca atttaatagt gctattggca aaattcaaga
     3601 ctcactttct tccacagcaa gtgcacttgg aaaacttcaa gatgtggtca accaaaatgc
     3661 acaagcttta aacacgcttg ttaaacaact tagctccaat tttggtgcaa tttcaagtgt
     3721 tttaaatgat atcctttcac gtcttgacaa agttgaggct gaagtgcaaa ttgataggtt
     3781 gatcacaggc agacttcaaa gtttgcagac atatgtgact caacaattaa ttagagctgc
     3841 agaaatcaga gcttctgcta atcttgctgc tactaaaatg tcagagtgtg tacttggaca
     3901 atcaaaaaga gttgattttt gtggaaaggg ctatcatctt atgtccttcc ctcagtcagc
     3961 acctcatggt gtagtcttct tgcatgtgac ttatgtccct gcacaagaaa agaacttcac
     4021 aactgctcct gccatttgtc atgatggaaa agcacacttt cctcgtgaag gtgtctttgt
     4081 ttcaaatggc acacactggt ttgtaacaca aaggaatttt tatgaaccac aaatcattac
     4141 tacagacaac acatttgtgt ctggtaactg tgatgttgta ataggaattg tcaacaacac
     4201 agtttatgat cctttgcaac ctgaattaga ctcattcaag gaggagttag ataaatattt
     4261 taagaatcat acatcaccag atgttgattt aggtgacatc tctggcatta atgcttcagt
     4321 tgtaaacatt caaaaagaaa ttgaccgcct caatgaggtt gccaagaatt taaatgaatc
     4381 tctcatcgat ctccaagaac ttggaaagta tgagcagtat ataaaatggc catggtacat
     4441 ttggctaggt tttatagctg gcttgattgc catagtaatg gtgacaatta tgctttgctg
     4501 tatgaccagt tgctgtagtt gtctcaaggg ctgttgttct tgtggatcct gctgcaaatt
     4561 tgatgaagac gactctgagc cagtgctcaa aggagtcaaa ttacattaca cataatccgg
     4621 actcagatct cgatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg
     4681 agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt
     4741 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg
     4801 agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt
     4861 ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata
     4921 gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt
     4981 taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac
     5041 tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc
     5101 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     5161 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     5221 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     5281 cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     5341 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     5401 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     5461 ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa
     5521 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     5581 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc
     5641 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     5701 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     5761 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     5821 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     5881 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     5941 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6001 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6061 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6121 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6181 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6241 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6301 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6361 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6421 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     6481 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     6541 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     6601 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     6661 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     6721 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     6781 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     6841 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     6901 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     6961 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7021 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7081 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7141 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7201 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     7261 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     7321 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     7381 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     7441 aaaaagggaa taagggcgac acggaaatgt tgaatactca t
//
