LOCUS       sequence-292288-        9554 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for CRISPR-generated knock-in line that expresses 
            optogenetic activator CsChrimson-tdTomato by knocking-in fragment 
            into the coding sequence of the endogenous Aedes aegypti fru gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Basrur NS, De Obaldia ME, Morita T, Herre M, von Heynitz RK, 
            Tsitohay YN, Vosshall LB
  TITLE     Fruitless mutant male mosquitoes gain attraction to human odor.
  JOURNAL   Elife. 2020 Dec 7;9. pii: 63982. doi: 10.7554/eLife.63982.
  PUBMED    33284111
REFERENCE   2  (bases 1 to 9554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-12-7"; volume: "9. pii: 63982. doi"; pages: " 
            10.7554/eLife.63982"
COMMENT     Sequence Label: fru-T2A-CsChrimson-tdTomato HDR plasmid
FEATURES             Location/Qualifiers
     source          1..9554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      290..299
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(296..298,299..301,302..1015)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(341..362)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(602..621)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     949..970
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1138..1259
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2730..2752)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2852..2871
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3079..3096
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3080..3096
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3188..3204)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3188..3204)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3201..3223)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3212..3228
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3236..3242,3243..3260,3261..3266))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3281..3302
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3419..3436)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3590..4178)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3670..3689)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4349..5140,5141..5209))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4972..4991
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5210..5314)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5382..5400
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             6207..6260
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     intron          8933..8998
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9128..9148
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9420..9554
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gatctggccg gccgtttaaa aattcgagct cgcccgggga tctaattcaa ttagagacta
       61 atwcaattag agctaattca attaggatcc aagcttatcg atttcgaacc ctcgaccgcc
      121 ggagtataaa tagaggcgct tcgtctacgg agcgacaatt caattcaaac aagcaaagtg
      181 aacacgtcgc taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa gctaaacaat
      241 cggggtaccg ctagagtcga cggtaccgcg ggcccgggat ccaccggtcg ccaccatggt
      301 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
      361 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
      421 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
      481 gaccaccttc ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
      541 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
      601 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
      661 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
      721 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
      781 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
      841 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
      901 gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
      961 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg
     1021 ccgcgactct agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     1081 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     1141 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     1201 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
     1261 agcttatcga tacgcgtacg gcgcgagcgg ggtttccata gacacaaaat taaggcgaga
     1321 aatcgccaac cgtataacca ttgctgatct taccagcaca catcatcatg gaatcttctg
     1381 accgatcgcg tcatcgatcg tgtcttgtac atttaccatg cgagcttgaa tcaagggaaa
     1441 cacacatatg tactataaaa aaataaagaa atgataaatc ttgcaatgaa tataccttat
     1501 ctaataaata taacaatcaa aaaaacgcca gccgtctatc aaagatgaga aatcaaatac
     1561 acctgcaaac atttgatatc ctgtaaaaaa aacgaaaagc agtactaata gacaaatacg
     1621 ataataatat acgataataa cgtcaaacgt agcagcaatt caggttaacc attttatcaa
     1681 aaccgttata tttaaacttt taaaagtagt tgaagtgaga aataaaaagc atatctttaa
     1741 ccgttcctcc tggagaaagt gtacaaggaa aagtatacaa ttgtgaactg taaaaaaagt
     1801 tgtaacaaac gagctgataa atattgctta aattttcgaa atatacattt caaaaagact
     1861 aactataagt gcacagaaac tttcttcgct tcagtagtgt ttcgtcgaac aatacgcggt
     1921 agtcatgctt tgcttcctgt agtgatattc gccagtttat catcataaca aaaagttaac
     1981 ccagttaaag caaaaaagaa cattaacaag acatgtaaat gatccgtaga gtttccgagt
     2041 acaaaacgaa cttagctaaa caaaagaaaa acgaatgaat tgcaaccagt gaatcatata
     2101 gcatcattta acccaattta ttaccgatat aaacctagtt acgtaatttg tacattaaaa
     2161 caaaagagtt aaaccaaact atttaaccct cttccaacaa tttaaacaaa aaaaaaactc
     2221 cccgcgtaca attgatcgat tcaagcatct aaactctcac caaactaaaa aaaaatgtga
     2281 cgtttccctt gcaacgaaca accatccgaa actggcaacg cttccgtgtt cgtcaacgaa
     2341 cgccacatcc gaaaaatctg cctgtcagat catcaatcta ctgttctatc cgaaatctac
     2401 cggcgtttca cgccgcatct actagtgcac ctgtcaacat ccgagatacg aagcacgggc
     2461 gcaaagcgtt tgccagcact tctccctcaa tcagcaaatc atcacgtaaa tctgggccac
     2521 tgtactgtca gcggccgcag taaccatggg gcggtttagc cgacctttaa cggctggtaa
     2581 gtactttggt gggagtcact cacgacggtt tttttttttt gggtaactaa actccggtag
     2641 acaatcatgg tggtctcctt ggtgaatgca aatcggaaaa atggcttcgc cactgtttgc
     2701 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     2761 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     2821 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     2881 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     2941 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     3001 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     3061 ttttccaagt cacgacgttg taaaacgacg gccagtgaat tcgagctcgg tacccgggga
     3121 tccgcgaggg cgatgccacc tacggcctag agtcgacctg caggcatgca agcttggcgt
     3181 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     3241 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     3301 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     3361 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     3421 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     3481 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     3541 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     3601 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3661 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3721 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3781 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3841 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3901 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3961 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     4021 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     4081 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     4141 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     4201 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     4261 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     4321 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     4381 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     4441 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     4501 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     4561 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     4621 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     4681 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     4741 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     4801 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     4861 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     4921 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     4981 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     5041 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     5101 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     5161 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     5221 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     5281 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     5341 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     5401 ctcgctcatt attattggta ccggaatgga ggctatccat tccaatgatg gaccggaata
     5461 aatcttcttt tcccagcagc gcatttgcat cgccgtatac tattttcaca tcttgttttg
     5521 gggactctcc atatacctta tctagactct catagaactc atccttcatg tcatccggct
     5581 tatcgttctt tggcacatat atagtgaaca ttttgtagat gaagaacttg tccttcattt
     5641 acaacacaca gattcggttg cttaacggtt tccatcaaat aactcgcgtc atctgcttcg
     5701 caatcactat gaagccaact ccacgttggt attgctctgt tgccgccgct gtagtagatg
     5761 tggtacttga atgaagtgtt ggcgatgcac cgtttcgtgt tcgtagaaag ccaccggaag
     5821 tatgattaac tgataattta attacttgca tatctgaaaa tgataaaaat catatggaaa
     5881 tgtcggaaaa ataaaaacaa tcagtacttt ccatggatta ctgagcctaa ttgaatatct
     5941 agatataatt tatatggaaa acaaactctc gatgaactca aacatatgtt gaccataact
     6001 ttgtagcacc taattgattt attttccatt tcctctcctt ttgtttcagc tccttcaagt
     6061 tactgttgca agttctcaat agtatgtcat cgccgcctgc aatgcccaat tacaataacc
     6121 gttacccggc attgaacggc tatccccaga tcaacggtgt cgacccggca gaacccatcg
     6181 attgtcgtaa gcttcaatcg cacggagagg gcagaggaag tcttctaaca tgcggtgacg
     6241 tggaggagaa tcccggccct atgagcagac tggtcgccgc ttcttggctg ctggctctcc
     6301 tcctctgcgg aattaccagc acaacaacag cctctagcgc cccagcagct tcttctacag
     6361 acggaacagc cgccgcagca gtgtctcact acgccatgaa cggcttcgac gagctggcta
     6421 aaggagccgt ggtgccagaa gaccactttg tctgcggacc agccgacaag tgctattgct
     6481 ccgcttggct gcacagcaga ggcacaccag gagaaaagat cggcgcccag gtctgccagt
     6541 ggattgcttt cagcatcgcc atcgccctgc tgacattcta cggcttcagc gcctggaagg
     6601 ccacttgcgg ttgggaggag gtctacgtct gttgcgtcga ggtgctgttc gtgaccctgg
     6661 agatcttcaa ggagttcagc agccccgcca cagtgtacct gtctaccggc aaccacgcct
     6721 attgcctgcg ctacttcgag tggctgctgt cttgccccgt gatcctgatc aagctgagca
     6781 acctgagcgg cctgaagaac gactacagca agcggaccat gggcctgatc gtgtcttgcg
     6841 tgggaatgat cgtgttcggc atggccgcag gactggctac cgattggctc aagtggctgc
     6901 tgtatatcgt gtcttgcatc tacggcggct acatgtactt ccaggccgcc aagtgctacg
     6961 tggaagccaa ccacagcgtg cctaaaggcc attgccgcat ggtcgtgaag ctgatggcct
     7021 acgcttactt cgcctcttgg ggcagctacc caatcctctg ggcagtggga ccagaaggac
     7081 tgctgaagct gagcccttac gccaacagca tcggccacag catctgcgac atcatcgcca
     7141 aggagttttg gaccttcctg gcccaccacc tgaggatcaa gatccacgag cacatcctga
     7201 tccacggcga catccggaag accaccaaga tggagatcgg aggcgaggag gtggaagtgg
     7261 aagagttcgt ggaggaggag gacgaggaca cagtggcgat cgctgctgcc gccgcggcgg
     7321 ccaagagcag gatcaccagc gagggcgagt acatccccct ggaccagatc gacatcaacg
     7381 gaggttcagg cggaaccggt gttagcaagg gtgaagaggt gattaaagag tttatgcgtt
     7441 ttaaagtacg catggagggt agcatgaacg gtcatgagtt tgagatcgag ggcgaaggcg
     7501 aaggtcgtcc gtacgagggt acgcagaccg ctaaactgaa agtaaccaaa ggcggtcctc
     7561 tgccgttcgc gtgggacatc ctgtccccgc agtttatgta tggttccaag gcctacgtca
     7621 aacacccagc cgatatcccg gactacaaga agctgtcttt cccggagggt ttcaagtggg
     7681 agcgtgtaat gaactttgag gacggtggcc tggttaccgt cacccaggat agctctctgc
     7741 aagatggcac cctgatctac aaggtgaaga tgcgtggtac gaactttccg ccggatggcc
     7801 cggtgatgca gaaaaagacg atgggatggg aagcatctac cgagcgcctg tatccgcgtg
     7861 acggtgttct gaaaggtgag atccaccagg cgctgaaact gaaggacggc ggtcactacc
     7921 tggttgaatt taagacgatc tatatggcta aaaagccggt tcaactgcca ggttactact
     7981 acgtggacac caaactggat atcacgtctc acaacgaaga ttataccatc gtcgaacagt
     8041 atgaacgtag cgagggccgt caccacctgt tcctgggcca cggtactggt tctacgggtt
     8101 ctggttcctc tggcactgcg tcttccgaag acaacaacat ggcggtgatc aaagagttca
     8161 tgaggttcaa agtacgtatg gaaggttcta tgaacggcca tgagttcgag attgaaggtg
     8221 agggcgaggg tcgcccatac gagggcactc agaccgcaaa gctgaaagtt accaaaggtg
     8281 gcccactgcc ttttgcgtgg gatatcctgt ctccgcagtt catgtacggt tccaaagcct
     8341 acgtaaaaca cccggcagac atcccggatt acaaaaagct gtccttccca gagggcttca
     8401 aatgggaacg tgttatgaat ttcgaagatg gcggcctggt cactgttacg caggacagca
     8461 gcctgcaaga cggtactctg atttacaaag tgaaaatgcg tggcactaac ttcccgcctg
     8521 acggtccagt aatgcagaag aaaactatgg gctgggaagc tagcaccgaa cgtctgtacc
     8581 cgcgcgacgg tgtgctgaaa ggcgagattc accaggccct gaagctgaag gatggtggtc
     8641 actatctggt tgagttcaaa actatctaca tggcaaaaaa gcctgtacaa ctgccgggct
     8701 attactacgt tgacactaaa ctggacatca cctcccacaa cgaggattat actattgttg
     8761 aacagtacga gcgttctgaa ggtcgccatc atctgtttct gtatggtatg gacgaactgt
     8821 ataaattctg ctacgagaac gaggtgtaat ctagaggatc tttgtgaagg aaccttactt
     8881 ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
     8941 aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat
     9001 tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct
     9061 gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc
     9121 tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct
     9181 aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac
     9241 cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt tgatgtatag
     9301 tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     9361 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     9421 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     9481 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     9541 atcatgtctg gatc
//
