LOCUS       sequence-292293-       13303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for FLAG-tagged mGluR3 fused to the 
            N-terminal half of split fluorescent Venus (VN).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13303)
  TITLE     Mammalian expression plasmids for production of metabotropic 
            glutamate receptor type 3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCEP4_HLA-flag-GRM3 C127A-VN
FEATURES             Location/Qualifiers
     source          1..13303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     110..132
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     polyA_signal    275..409
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(312..331)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     366..385
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(432..890,891..893))
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYLTA"
     primer_bind     571..590
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     830..851
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(3453..3476)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      3551..3560
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3588..3612)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(3609..3812)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3638..3658)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3813..4192
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    4846..4894
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4919..4938
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4936..5973)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     promoter        complement(6016..6161)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     rep_origin      complement(6592..7180)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6672..6691)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7351..8142,8143..8211))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7974..7993
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8212..8316)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8384..8402
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             8837..10762
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGXGGAGAGGGXGXGGGXGGAGGXGXGGGAGAGGGAGGAGAGGGX
                     GGXGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGXAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGXGGGXXGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGXGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     rep_origin      11064..12853
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tctagagtcg acctcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
       61 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
      121 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagccgga tcataatcag
      181 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
      241 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
      301 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
      361 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc ggccttgccg
      421 gcctcgagtt aggcggtcag atagacgttg tggctgttgt agttgtactc cagcttgtgc
      481 cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg
      541 gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag
      601 atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc
      661 atgtggtcgg ggtagcgggc gaagcactgc aggccgtagc ccagggtggt cacgagggtg
      721 ggccagggca cgggcagctt gccggtggtg cagatcagct tcagggtcag cttgccgtag
      781 gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc
      841 agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct caccagagat
      901 gaggtggtgg agtcgaggac ttcccgccca ttgcacaccg ttggcacata cgtgcttgca
      961 gaggactgag agtatgtggt cccagttcca ctgacactga acctgttgag gtgcagtctg
     1021 tgtgtgacaa cattcttctg gggttgaaac aggatgatgt gaaccttggg tgcaaacaaa
     1081 cagcccaaga ccacaaagcc actcaggctg acagagatgc acatggttgt cgtctgcact
     1141 ctgtagtcac ttgatgtcac ataaaatata gggaggaagg ccaaccagat gatgcacgtg
     1201 gtgtacatgg taaaacctat gaacttagct tcgttgaaat tttctgggca cttccgcgtt
     1261 ttgaaggcgt acacagtgca taagatcacc aggatcacat cgtaggtaag agagatcaac
     1321 atgctggaat ctttgacatt gcattttagg atgactgttt cccgcttctc tgcaagggta
     1381 tacctcctgg tgcctggggc ctccaggatg agccacacag acaccatcac aatttgcacc
     1441 aggatcagac ccaggcagat gaaaacctga gaactggggc tgatgaattt tggcctctga
     1501 gcgccattct tgaccccatc gaagatgcgg gcaatgcagt ttgtcttggt cagcagggct
     1561 gagtaacaga tagcgaagga actccccagc ccgagtcggc gcaatgcaca gatgactggt
     1621 gatggcttgg caatgaagaa gaatgtcatg cagtatgaca ggccaacccc aaacaataag
     1681 atgtagcaga gttctcggcc cgatgctttg accaagggtg tgttgttgtg cttgataaaa
     1741 acagttacaa ccatgcatgt acacataaaa cccagacagg caatggtgac tgggccaatg
     1801 gcccaggcgt cttcccacct gatgtagtcc tcaggaaggt catagcatcc agttaggtct
     1861 gcagtgggcc actgtccaga cccacaatcc atacaggtaa actcatcagc caggtattcg
     1921 tacggttcac aggggatgca aatccagcag cagacatccc ctggttgcat attcttcatt
     1981 tcattggggg cacaggggtc gctgcactgg gaagtgggga ctgagttccg ggaccagtgg
     2041 atagagttga catctagcga taaggtttct gcccagtgac caactttcaa gtaggaatac
     2101 tttccaccta cattttggaa attgaacacg ttgtatcgcc ccattccatc tccaaaagtg
     2161 tcaaacttga ctatgctatc tgcatcttta tttgggttga atggagccgt gaagttgatt
     2221 ttcagcaagt aatccttgta caacttcttc ccatccagga tcttcatagc atcacaaagc
     2281 ttggtagtgt tgggacagag ggtgcgctgc attttgtgca aagcgtgggc catggcatac
     2341 accgcgttca ccacaaacat gatcttggac tcttgctcgt agttgctgct gtcgatggcc
     2401 aggtgcttgt cgcagacgcg cctgtggttg cgtttgttct ggaggctgca ctgaaacttt
     2461 tgctcccaga agtcccggaa ccaggggttg cggtggttgt tgtaggggtt gaggctctgg
     2521 aagtagcggt cgaactggcg gacaggctgg gaggccagct ccagggtgat ggcgccgtag
     2581 gccacatgct cgctgccctt gatgatgctc tcctgcgcgc cccagccgtc gctggccacc
     2641 caggtgaagg aggcattggc gcggctggcg gctgcaatga gctcccgcga gtcgtcgctg
     2701 cgcatgaaga ggaccacgac gcgcgcgttg ggcttctgca acagttctcg gatcacgctg
     2761 tcgtaggact tgcggatgtt ggagcggccc accttctccg ccgtagcgat gcagatgttg
     2821 cgcaggcggg cttcctgctc gaaggcctcg atccctgtct ccccgtaatc accctcggag
     2881 gctactgtgg acacgtaggt ccagttgaag aagcgcaaga tctcagccat ggctttggcc
     2941 tggtagaagt cggggggcac ggtcctggca aagtaatcat agcgcgactt atcactgagt
     3001 ttggcgctgg tggatgcgta gctgatctga gggatctgga agagccgcag caggtttgcc
     3061 acctgtatgg aaacactgct ataagagcca ccaatgaccc ctgcaatgag aagtgggatg
     3121 ttttcttgaa tggcatagga tccatcagga gccatatact cagcttcatc cacttttgtc
     3181 aaagatgccc tgacaaactc cagtgattgc tccaatgcat aggtatccct tgaacatgta
     3241 tccaaaatgt gaacacccaa cttcactcct ggtagcaagt aatcatcttt gttgatttca
     3301 tcaatagcaa acaacatggc ttccaggcgt tgaatccctc ggtcttcatt gattcgccca
     3361 cattcttcag ttccagtgcc tttttcgtta ataggaaaca ggccccctaa aacaaggtca
     3421 ccttctattt taatctctct cctggatcct cccttgtcat catcgtcctt gtagtcggag
     3481 cccgcccagg tctgggtcag ggccagggcc cccgagagta gcaggacgag ggttcggggc
     3541 gccatgacgg ccatggtggc gctagcagct ggtacccagc ttctagagat ctgacggttc
     3601 actaaacgag ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc
     3661 aacggggcgg ggttattacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa
     3721 ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca
     3781 cgcccattgg tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag
     3841 atgtactgcc aagtaggaaa gtcccgtaag gtcatgtact gggcataatg ccaggcgggc
     3901 catttaccgt cattgacgtc aatagggggc ggacttggca tatgatacac ttgatgtact
     3961 gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt
     4021 ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt
     4081 cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga
     4141 actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg tcaacatggc
     4201 ggtcatattg gacatgagcc aatataaatg tacatattat gatatagata caacgtatgc
     4261 aatggccaat agccaatatt gatttatgct atataaccaa tgactaatat ggctaattgc
     4321 caatattgat tcaatgtata gatcttccat acctaccagt tctgcgcctg cagcaatggc
     4381 aacaacgttg cccggatctg cgatgataag ctgtcaaaca tgagaattgg tcgactagct
     4441 tggcacgcca gaaatccgcg cggtggtttt tgggggtcgg gggtgtttgg cagccacaga
     4501 cgcccggtgt tcgtgtcgcg ccagtacatg cggtccatgc ccaggccatc caaaaaccat
     4561 gggtctgtct gctcagtcca gtcgtggacc tgaccccacg caacgcccaa aataataacc
     4621 cccacgaacc ataaaccatt ccccatgggg gaccccgtcc ctaacccacg gggccagtgg
     4681 ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc tgggccttca cccgaacttg
     4741 gggggtgggg tggggaaaag gaagaaacgc gggcgtattg gccccaatgg ggtctcggtg
     4801 gggtatcgac agagtgccag ccctgggacc gaaccccgcg tttatgaaca aacgacccaa
     4861 cacccgtgcg ttttattctg tctttttatt gccgtcatag cgcgggttcc ttccggtatt
     4921 gtctccttcc gtgtttcagt tagcctcccc catctcccga tccggacgag tgctggggcg
     4981 tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct
     5041 gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg
     5101 accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc
     5161 aagaccaatg cggagcatat acgcccggag ccgcggcgat cctgcaagct ccggatgcct
     5221 ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat
     5281 gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt
     5341 tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg
     5401 gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc
     5461 actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca
     5521 tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc
     5581 gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga ccggctgcag
     5641 aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg cacggcggga
     5701 gatgcaatag gtcaggctct cgctgaattc cccaatgtca agcacttccg gaatcgggag
     5761 cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct
     5821 atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc
     5881 gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc
     5941 gacagacgtc gcggtgagtt caggcttttt catatctcat tgccccccgg gatctgcggc
     6001 acgctgttga cgctgttaag cgggtcgctg cagggtcgct cggtgttcga ggccacacgc
     6061 gtcaccttaa tatgcgaagt ggacctcgga ccgcgccgcc ccgactgcat ctgcgtgttc
     6121 gaattcgcca atgacaagac gctgggcggg gtttgtgtca tcatagaact aaagacatgc
     6181 aaatatattt cttccgggga caccgccagc aaacgcgagc aacgggccac ggggatgaag
     6241 cagggcggca cctcgctaac ggattcacca ctccaagaat tggagccaat caattcttgc
     6301 ggagaactgt gaatgcgcaa accaaccctt ggcagaacat atccatcgcg tccgccatct
     6361 ccagcagccg cacgcggcgc atctcggggc cgacgcgctg ggctacgtct tgctggcgtt
     6421 cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg
     6481 gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct
     6541 tcaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6601 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6661 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6721 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6781 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6841 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     6901 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     6961 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7021 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7081 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7141 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7201 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7261 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     7321 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     7381 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     7441 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     7501 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     7561 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     7621 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt
     7681 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     7741 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7801 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     7861 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     7921 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac
     7981 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     8041 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     8101 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8161 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     8221 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     8281 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     8341 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     8401 gccctttcgt cttcaagaat tctcatgttt gacagcttat catcgataag ctgatcctca
     8461 caggccgcac ccagcttttc ttccgttgcc ccagtagcat ctctgtctgg tgaccttgaa
     8521 gaggaagagg aggggtcccg agaatcccca tccctaccgt ccagcaaaaa gggggacgag
     8581 gaatttgagg cctggcttga ggctcaggac gcaaatcttg aggatgttca gcgggagttt
     8641 tccgggctgc gagtaattgg tgatgaggac gaggatggtt cggaggatgg ggaattttca
     8701 gacctggatc tgtctgacag cgaccatgaa ggggatgagg gtgggggggc tgttggaggg
     8761 ggcaggagtc tgcactccct gtattcactg agcgtcgtct aataaagatg tctattgatc
     8821 tcttttagtg tgaatcatgt ctgacgaggg gccaggtaca ggacctggaa atggcctagg
     8881 agagaaggga gacacatctg gaccagaagg ctccggcggc agtggacctc aaagaagagg
     8941 gggtgataac catggacgag gacggggaag aggacgagga cgaggaggcg gaagaccagg
     9001 agccccgggc ggctcaggat cagggccaag acatagagat ggtgtccgga gaccccaaaa
     9061 acgtccaagt tgcattggct gcaaagggac ccacggtgga acaggagcag gagcaggagc
     9121 gggaggggca ggagcaggag gggcaggagc aggaggaggg gcaggagcag gaggaggggc
     9181 aggaggggca ggaggggsag gaggggcagg agcaggagga ggggsaggag saggaggagg
     9241 ggsaggaggr gcaggagggg saggagsagg aggrggggca ggagcaggag gaggggcagg
     9301 aggggcagga gcaggaggrg gggmaggagg rgsaggaggg gcaggagcag gaggaggggc
     9361 aggagcagga ggaggggcag gaggggcagg agcaggagga ggggcaggag gggcaggagg
     9421 ggcaggagca ggaggagggg caggagcrgg aggggcagga gsggcaggag gggcaggagc
     9481 aggaggggca ggagcaggag gaggggcagg aggggcagga ggggcaggag caggaggrgc
     9541 aggagcagga ggggcaggag caggaggggc aggagcagga ggggcaggag gggcaggagc
     9601 aggaggggsa ggagggggys gwkgaggagg ggcaggaggg gcaggagcag gaggaggggc
     9661 aggaggggca ggagcaggag gaggggcagg aggggcagga gcaggagggg saggaggggc
     9721 aggagcagga ggggcaggag gggcaggagc aggaggggca ggaggggcag gagcaggagg
     9781 aggggcagga gcaggagggg caggagcagg aggtggaggc cggggtcgag gaggcagtgg
     9841 aggccggggt cgaggaggta gtggaggccg gggtcgagga ggtagtggag gccgccgggg
     9901 tagaggacgt gaaagagcca gggggggaag tcgtgaaaga gccaggggga gaggtcgtgg
     9961 acgtggagaa aagaggccca ggagtcccag tagtcagtca tcatcatccg ggtctccacc
    10021 gcgcaggccc cctccaggta gaaggccatt tttccaccct gtaggggaag ccgattattt
    10081 tgaataccac caagaaggtg gcccagatgg tgagcctgac gtgcccccgg gagcgataga
    10141 gcagggcccc gcagatgacc caggagaagg cccaagcact ggaccccggg gtcagggtga
    10201 tggaggcagg cgcaaaaaag gagggtggtt tggaaagcat cgtggtcaag gaggttccaa
    10261 cccgaaattt gagaacattg cagaaggttt aagagctctc ctggctagga gtcacgtaga
    10321 aaggactacc gacgaaggaa cttgggtcgc cggtgtgttc gtatatggag gtagtaagac
    10381 ctccctttac aacctaaggc gaggaactgc ccttgctatt ccacaatgtc gtcttacacc
    10441 attgagtcgt ctcccctttg gaatggcccc tggacccggc ccacaacctg gcccgctaag
    10501 ggagtccatt gtctgttatt tcatggtctt tttacaaact catatatttg ctgaggtttt
    10561 gaaggatgcg attaaggacc ttgttatgac aaagcccgct cctacctgca atatcagggt
    10621 gactgtgtgc agctttgacg atggagtaga tttgcctccc tggtttccac ctatggtgga
    10681 aggggctgcc gcggagggtg atgacggaga tgacggagat gaaggaggtg atggagatga
    10741 gggtgaggaa gggcaggagt gatgtaactt gttaggagac gccctcaatc gtattaaaag
    10801 ccgtgtattc ccccgcacta aagaataaat ccccagtaga catcatgcgt gctgttggtg
    10861 tatttctggc catctgtctt gtcaccattt tcgtcctccc aacatggggc aattgggcat
    10921 acccatgttg tcacgtcact cagctccgcg ctcaacacct tctcgcgttg gaaaacatta
    10981 gcgacattta cctggtgagc aatcagacat gcgacggctt tagcctggcc tccttaaatt
    11041 cacctaagaa tgggagcaac cagcaggaaa aggacaagca gcgaaaattc acgccccctt
    11101 gggaggtggc ggcatatgca aaggatagca ctcccactct actactgggt atcatatgct
    11161 gactgtatat gcatgaggat agcatatgct acccggatac agattaggat agcatatact
    11221 acccagatat agattaggat agcatatgct acccagatat agattaggat agcctatgct
    11281 acccagatat aaattaggat agcatatact acccagatat agattaggat agcatatgct
    11341 acccagatat agattaggat agcctatgct acccagatat agattaggat agcatatgct
    11401 acccagatat agattaggat agcatatgct atccagatat ttgggtagta tatgctaccc
    11461 agatataaat taggatagca tatactaccc taatctctat taggatagca tatgctaccc
    11521 ggatacagat taggatagca tatactaccc agatatagat taggatagca tatgctaccc
    11581 agatatagat taggatagcc tatgctaccc agatataaat taggatagca tatactaccc
    11641 agatatagat taggatagca tatgctaccc agatatagat taggatagcc tatgctaccc
    11701 agatatagat taggatagca tatgctatcc agatatttgg gtagtatatg ctacccatgg
    11761 caacattagc ccaccgtgct ctcagcgacc tcgtgaatat gaggaccaac aaccctgtgc
    11821 ttggcgctca ggcgcaagtg tgtgtaattt gtcctccaga tcgcagcaat cgcgccccta
    11881 tcttggcccg cccacctact tatgcaggta ttccccgggg tgccattagt ggttttgtgg
    11941 gcaagtggtt tgaccgcagt ggttagcggg gttacaatca gccaagttat tacaccctta
    12001 ttttacagtc caaaaccgca gggcggcgtg tgggggctga cgcgtgcccc cactccacaa
    12061 tttcaaaaaa aagagtggcc acttgtcttt gtttatgggc cccattggcg tggagccccg
    12121 tttaattttc gggggtgtta gagacaacca gtggagtccg ctgctgtcgg cgtccactct
    12181 ctttcccctt gttacaaata gagtgtaaca acatggttca cctgtcttgg tccctgcctg
    12241 ggacacatct taataacccc agtatcatat tgcactagga ttatgtgttg cccatagcca
    12301 taaattcgtg tgagatggac atccagtctt tacggcttgt ccccacccca tggatttcta
    12361 ttgttaaaga tattcagaat gtttcattcc tacactagta tttattgccc aaggggtttg
    12421 tgagggttat attggtgtca tagcacaatg ccaccactga accccccgtc caaattttat
    12481 tctgggggcg tcacctgaaa ccttgttttc gagcacctca catacacctt actgttcaca
    12541 actcagcagt tattctatta gctaaacgaa ggagaatgaa gaagcaggcg aagattcagg
    12601 agagttcact gcccgctcct tgatcttcag ccactgccct tgtgactaaa atggttcact
    12661 accctcgtgg aatcctgacc ccatgtaaat aaaaccgtga cagctcatgg ggtgggagat
    12721 atcgctgttc cttaggaccc ttttactaac cctaattcga tagcatatgc ttcccgttgg
    12781 gtaacatatg ctattgaatt agggttagtc tggatagtat atactactac ccgggaagca
    12841 tatgctaccc gtttagggtt aacaaggggg ccttataaac actattgcta atgccctctt
    12901 gagggtccgc ttatcggtag ctacacaggc ccctctgatt gacgttggtg tagcctcccg
    12961 tagtcttcct gggcccctgg gaggtacatg tcccccagca ttggtgtaag agcttcagcc
    13021 aagagttaca cataaaggca atgttgtgtt gcagtccaca gactgcaaag tctgctccag
    13081 gatgaaagcc actcagtgtt ggcaaatgtg cacatccatt tataaggatg tcaactacag
    13141 tcagagaacc cctttgtgtt tggtcccccc ccgtgtcaca tgtggaacag ggcccagttg
    13201 gcaagttgta ccaaccaact gaagggatta catgcactgc cccgcgaaga aggggcagag
    13261 atgtcgtagt caggtttagt tcgtccgggg cggggatcga tcc
//
