LOCUS       sequence-292295-       13099 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for c-myc-tagged mGluR3 fused to the 
            C-terminal half of split fluorescent Venus (VC).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13099)
  TITLE     Mammalian expression plasmids for production of metabotropic 
            glutamate receptor type 3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCEP4_HLA-myc-GRM3 C127A-VC
FEATURES             Location/Qualifiers
     source          1..13099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     110..132
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     polyA_signal    275..409
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(312..331)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     366..385
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(432..683)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(474..495)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(3243..3272)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      3347..3356
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3384..3408)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(3405..3608)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3434..3454)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3609..3988
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    4642..4690
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4715..4734
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4732..5769)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     promoter        complement(5812..5957)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     rep_origin      complement(6388..6976)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6468..6487)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7147..7938,7939..8007))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7770..7789
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8008..8112)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8180..8198
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             8633..10558
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGXGGXGXGGGXGXGGGXGGXGGAGXGGGXGAGGGXGGXGXGGGX
                     GGXGGXGAGGGAGAGGGAGGXGXGGGXGGXGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGXGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     rep_origin      10860..12649
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tctagagtcg acctcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
       61 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
      121 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagccgga tcataatcag
      181 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
      241 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
      301 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
      361 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc ggccttgccg
      421 gcctcgagtt acttgtacag ctcgtccata ccgagagtga tcccggcggc ggtcacgaac
      481 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagctt ggactggtag
      541 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
      601 tagtggtcgg cgagctgcac gccgccgtcc tcgatgttgt ggcggatctt gaagttggcc
      661 ttgatgccgt tcttctgctt gtccagagat gaggtggtgg agtcgaggac ttcccgccca
      721 ttgcacaccg ttggcacata cgtgcttgca gaggactgag agtatgtggt cccagttcca
      781 ctgacactga acctgttgag gtgcagtctg tgtgtgacaa cattcttctg gggttgaaac
      841 aggatgatgt gaaccttggg tgcaaacaaa cagcccaaga ccacaaagcc actcaggctg
      901 acagagatgc acatggttgt cgtctgcact ctgtagtcac ttgatgtcac ataaaatata
      961 gggaggaagg ccaaccagat gatgcacgtg gtgtacatgg taaaacctat gaacttagct
     1021 tcgttgaaat tttctgggca cttccgcgtt ttgaaggcgt acacagtgca taagatcacc
     1081 aggatcacat cgtaggtaag agagatcaac atgctggaat ctttgacatt gcattttagg
     1141 atgactgttt cccgcttctc tgcaagggta tacctcctgg tgcctggggc ctccaggatg
     1201 agccacacag acaccatcac aatttgcacc aggatcagac ccaggcagat gaaaacctga
     1261 gaactggggc tgatgaattt tggcctctga gcgccattct tgaccccatc gaagatgcgg
     1321 gcaatgcagt ttgtcttggt cagcagggct gagtaacaga tagcgaagga actccccagc
     1381 ccgagtcggc gcaatgcaca gatgactggt gatggcttgg caatgaagaa gaatgtcatg
     1441 cagtatgaca ggccaacccc aaacaataag atgtagcaga gttctcggcc cgatgctttg
     1501 accaagggtg tgttgttgtg cttgataaaa acagttacaa ccatgcatgt acacataaaa
     1561 cccagacagg caatggtgac tgggccaatg gcccaggcgt cttcccacct gatgtagtcc
     1621 tcaggaaggt catagcatcc agttaggtct gcagtgggcc actgtccaga cccacaatcc
     1681 atacaggtaa actcatcagc caggtattcg tacggttcac aggggatgca aatccagcag
     1741 cagacatccc ctggttgcat attcttcatt tcattggggg cacaggggtc gctgcactgg
     1801 gaagtgggga ctgagttccg ggaccagtgg atagagttga catctagcga taaggtttct
     1861 gcccagtgac caactttcaa gtaggaatac tttccaccta cattttggaa attgaacacg
     1921 ttgtatcgcc ccattccatc tccaaaagtg tcaaacttga ctatgctatc tgcatcttta
     1981 tttgggttga atggagccgt gaagttgatt ttcagcaagt aatccttgta caacttcttc
     2041 ccatccagga tcttcatagc atcacaaagc ttggtagtgt tgggacagag ggtgcgctgc
     2101 attttgtgca aagcgtgggc catggcatac accgcgttca ccacaaacat gatcttggac
     2161 tcttgctcgt agttgctgct gtcgatggcc aggtgcttgt cgcagacgcg cctgtggttg
     2221 cgtttgttct ggaggctgca ctgaaacttt tgctcccaga agtcccggaa ccaggggttg
     2281 cggtggttgt tgtaggggtt gaggctctgg aagtagcggt cgaactggcg gacaggctgg
     2341 gaggccagct ccagggtgat ggcgccgtag gccacatgct cgctgccctt gatgatgctc
     2401 tcctgcgcgc cccagccgtc gctggccacc caggtgaagg aggcattggc gcggctggcg
     2461 gctgcaatga gctcccgcga gtcgtcgctg cgcatgaaga ggaccacgac gcgcgcgttg
     2521 ggcttctgca acagttctcg gatcacgctg tcgtaggact tgcggatgtt ggagcggccc
     2581 accttctccg ccgtagcgat gcagatgttg cgcaggcggg cttcctgctc gaaggcctcg
     2641 atccctgtct ccccgtaatc accctcggag gctactgtgg acacgtaggt ccagttgaag
     2701 aagcgcaaga tctcagccat ggctttggcc tggtagaagt cggggggcac ggtcctggca
     2761 aagtaatcat agcgcgactt atcactgagt ttggcgctgg tggatgcgta gctgatctga
     2821 gggatctgga agagccgcag caggtttgcc acctgtatgg aaacactgct ataagagcca
     2881 ccaatgaccc ctgcaatgag aagtgggatg ttttcttgaa tggcatagga tccatcagga
     2941 gccatatact cagcttcatc cacttttgtc aaagatgccc tgacaaactc cagtgattgc
     3001 tccaatgcat aggtatccct tgaacatgta tccaaaatgt gaacacccaa cttcactcct
     3061 ggtagcaagt aatcatcttt gttgatttca tcaatagcaa acaacatggc ttccaggcgt
     3121 tgaatccctc ggtcttcatt gattcgccca cattcttcag ttccagtgcc tttttcgtta
     3181 ataggaaaca ggccccctaa aacaaggtca ccttctattt taatctctct cctggatcct
     3241 cccaagtcct cttcagaaat aagcttttgt tcggagcccg cccaggtctg ggtcagggcc
     3301 agggcccccg agagtagcag gacgagggtt cggggcgcca tgacggccat ggtggcgcta
     3361 gcagctggta cccagcttct agagatctga cggttcacta aacgagctct gcttatatag
     3421 acctcccacc gtacacgcct accgcccatt tgcgtcaacg gggcggggtt attacgacat
     3481 tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg
     3541 agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattggtgta ctgccaaaac
     3601 cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc
     3661 cgtaaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata
     3721 gggggcggac ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa
     3781 tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc
     3841 attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta
     3901 agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac
     3961 tattaataac tagtcaataa tcaatgtcaa catggcggtc atattggaca tgagccaata
     4021 taaatgtaca tattatgata tagatacaac gtatgcaatg gccaatagcc aatattgatt
     4081 tatgctatat aaccaatgac taatatggct aattgccaat attgattcaa tgtatagatc
     4141 ttccatacct accagttctg cgcctgcagc aatggcaaca acgttgcccg gatctgcgat
     4201 gataagctgt caaacatgag aattggtcga ctagcttggc acgccagaaa tccgcgcggt
     4261 ggtttttggg ggtcgggggt gtttggcagc cacagacgcc cggtgttcgt gtcgcgccag
     4321 tacatgcggt ccatgcccag gccatccaaa aaccatgggt ctgtctgctc agtccagtcg
     4381 tggacctgac cccacgcaac gcccaaaata ataaccccca cgaaccataa accattcccc
     4441 atgggggacc ccgtccctaa cccacggggc cagtggctat ggcagggcct gccgccccga
     4501 cgttggctgc gagccctggg ccttcacccg aacttggggg gtggggtggg gaaaaggaag
     4561 aaacgcgggc gtattggccc caatggggtc tcggtggggt atcgacagag tgccagccct
     4621 gggaccgaac cccgcgttta tgaacaaacg acccaacacc cgtgcgtttt attctgtctt
     4681 tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt ttcagttagc
     4741 ctcccccatc tcccgatccg gacgagtgct ggggcgtcgg tttccactat cggcgagtac
     4801 ttctacacag ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg tacgcccgac
     4861 agtcccggct ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc
     4921 gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga gcatatacgc
     4981 ccggagccgc ggcgatcctg caagctccgg atgcctccgc tcgaagtagc gcgtctgctg
     5041 ctccatacaa gccaaccacg gcctccagaa gaagatgttg gcgacctcgt attgggaatc
     5101 cccgaacatc gcctcgctcc agtcaatgac cgctgttatg cggccattgt ccgtcaggac
     5161 attgttggag ccgaaatccg cgtgcacgag gtgccggact tcggggcagt cctcggccca
     5221 aagcatcagc tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt
     5281 ttgccagtga tacacatggg gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta
     5341 ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa gatcggccgc
     5401 agcgatcgca tccatggcct ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg
     5461 caggtcttgc aacgtgacac cctgtgcacg gcgggagatg caataggtca ggctctcgct
     5521 gaattcccca atgtcaagca cttccggaat cgggagcgcg gccgatgcaa agtgccgata
     5581 aacataacga tctttgtaga aaccatcggc gcagctattt acccgcagga catatccacg
     5641 ccctcctaca tcgaagctga aagcacgaga ttcttcgccc tccgagagct gcatcaggtc
     5701 ggagacgctg tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg
     5761 ctttttcata tctcattgcc ccccgggatc tgcggcacgc tgttgacgct gttaagcggg
     5821 tcgctgcagg gtcgctcggt gttcgaggcc acacgcgtca ccttaatatg cgaagtggac
     5881 ctcggaccgc gccgccccga ctgcatctgc gtgttcgaat tcgccaatga caagacgctg
     5941 ggcggggttt gtgtcatcat agaactaaag acatgcaaat atatttcttc cggggacacc
     6001 gccagcaaac gcgagcaacg ggccacgggg atgaagcagg gcggcacctc gctaacggat
     6061 tcaccactcc aagaattgga gccaatcaat tcttgcggag aactgtgaat gcgcaaacca
     6121 acccttggca gaacatatcc atcgcgtccg ccatctccag cagccgcacg cggcgcatct
     6181 cggggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt
     6241 ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc aggccatgct
     6301 gtccaggcag gtagatgacg accatcaggg acagcttcaa ggccagcaaa aggccaggaa
     6361 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6421 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6481 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6541 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     6601 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6661 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     6721 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6781 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     6841 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     6901 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     6961 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7021 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7081 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7141 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7201 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7261 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7321 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7381 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7441 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7501 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7561 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     7621 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     7681 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     7741 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa
     7801 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     7861 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     7921 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     7981 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8041 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8101 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     8161 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaattctc
     8221 atgtttgaca gcttatcatc gataagctga tcctcacagg ccgcacccag cttttcttcc
     8281 gttgccccag tagcatctct gtctggtgac cttgaagagg aagaggaggg gtcccgagaa
     8341 tccccatccc taccgtccag caaaaagggg gacgaggaat ttgaggcctg gcttgaggct
     8401 caggacgcaa atcttgagga tgttcagcgg gagttttccg ggctgcgagt aattggtgat
     8461 gaggacgagg atggttcgga ggatggggaa ttttcagacc tggatctgtc tgacagcgac
     8521 catgaagggg atgagggtgg gggggctgtt ggagggggca ggagtctgca ctccctgtat
     8581 tcactgagcg tcgtctaata aagatgtcta ttgatctctt ttagtgtgaa tcatgtctga
     8641 cgaggggcca ggtacaggac ctggaaatgg cctaggagag aagggagaca catctggacc
     8701 agaaggctcc ggcggcagtg gacctcaaag aagagggggt gataaccatg gacgaggacg
     8761 gggaagagga cgaggacgag gaggcggaag accaggagcc ccgggcggct caggatcagg
     8821 gccaagacat agagatggtg tccggagacc ccaaaaacgt ccaagttgca ttggctgcaa
     8881 agggacccac ggtggaacag gagcaggagc aggagcggga ggggcaggag caggaggggc
     8941 aggagcagga ggaggggcag gagcaggagg aggggcagga ggggcaggag gggsaggagg
     9001 ggsaggagsa ggaggagggg saggagsagg aggaggggsr ggwggsgsag grggggcagg
     9061 agsaggagga ggrgsrggag caggaggagg ggsaggrggg gsaggrgsag gaggaggggs
     9121 aggaggggsa ggaggggsag gagcaggagg rggggcagga gcaggaggag gggcaggagg
     9181 rgsaggagsa ggaggagggg saggaggggs aggaggngca ggagcaggag gaggggcagg
     9241 agcaggaggg gcaggagggg caggaggggc aggagcagga ggggcaggag caggaggagg
     9301 ggcaggaggg gcaggagggg caggagcagg aggggcagga gcaggagggg caggagcagg
     9361 aggggcagga gcaggagggg caggaggggc aggagcagga ggggcaggrg gggcaggagc
     9421 aggaggggca ggaggggcag gagcaggagg aggggcagga ggggcaggag caggaggagg
     9481 ggcaggaggg gcaggagcag gaggggcagg aggggsagga gcaggagggg caggaggggc
     9541 aggagcagga ggggcaggag gggcaggagc aggaggaggg gcaggagcag gaggggcagg
     9601 agcaggaggt ggaggccggg gtcgaggagg cagtggaggc cggggtcgag gaggtagtgg
     9661 aggccggggt cgaggaggta gtggaggccg ccggggtaga ggacgtgaaa gagccagggg
     9721 gggaagtcgt gaaagagcca gggggagagg tcgtggacgt ggagaaaaga ggcccaggag
     9781 tcccagtagt cagtcatcat catccgggtc tccaccgcgc aggccccctc caggtagaag
     9841 gccatttttc caccctgtag gggaagccga ttattttgaa taccaccaag aaggtggccc
     9901 agatggtgag cctgacgtgc ccccgggagc gatagagcag ggccccgcag atgacccagg
     9961 agaaggccca agcactggac cccggggtca gggtgatgga ggcaggcgca aaaaaggagg
    10021 gtggtttgga aagcatcgtg gtcaaggagg ttccaacccg aaatttgaga acattgcaga
    10081 aggtttaaga gctctcctgg ctaggagtca cgtagaaagg actaccgacg aaggaacttg
    10141 ggtcgccggt gtgttcgtat atggaggtag taagacctcc ctttacaacc taaggcgagg
    10201 aactgccctt gctattccac aatgtcgtct tacaccattg agtcgtctcc cctttggaat
    10261 ggcccctgga cccggcccac aacctggccc gctaagggag tccattgtct gttatttcat
    10321 ggtcttttta caaactcata tatttgctga ggttttgaag gatgcgatta aggaccttgt
    10381 tatgacaaag cccgctccta cctgcaatat cagggtgact gtgtgcagct ttgacgatgg
    10441 agtagatttg cctccctggt ttccacctat ggtggaaggg gctgccgcgg agggtgatga
    10501 cggagatgac ggagatgaag gaggtgatgg agatgagggt gaggaagggc aggagtgatg
    10561 taacttgtta ggagacgccc tcaatcgtat taaaagccgt gtattccccc gcactaaaga
    10621 ataaatcccc agtagacatc atgcgtgctg ttggtgtatt tctggccatc tgtcttgtca
    10681 ccattttcgt cctcccaaca tggggcaatt gggcataccc atgttgtcac gtcactcagc
    10741 tccgcgctca acaccttctc gcgttggaaa acattagcga catttacctg gtgagcaatc
    10801 agacatgcga cggctttagc ctggcctcct taaattcacc taagaatggg agcaaccagc
    10861 aggaaaagga caagcagcga aaattcacgc ccccttggga ggtggcggca tatgcaaagg
    10921 atagcactcc cactctacta ctgggtatca tatgctgact gtatatgcat gaggatagca
    10981 tatgctaccc ggatacagat taggatagca tatactaccc agatatagat taggatagca
    11041 tatgctaccc agatatagat taggatagcc tatgctaccc agatataaat taggatagca
    11101 tatactaccc agatatagat taggatagca tatgctaccc agatatagat taggatagcc
    11161 tatgctaccc agatatagat taggatagca tatgctaccc agatatagat taggatagca
    11221 tatgctatcc agatatttgg gtagtatatg ctacccagat ataaattagg atagcatata
    11281 ctaccctaat ctctattagg atagcatatg ctacccggat acagattagg atagcatata
    11341 ctacccagat atagattagg atagcatatg ctacccagat atagattagg atagcctatg
    11401 ctacccagat ataaattagg atagcatata ctacccagat atagattagg atagcatatg
    11461 ctacccagat atagattagg atagcctatg ctacccagat atagattagg atagcatatg
    11521 ctatccagat atttgggtag tatatgctac ccatggcaac attagcccac cgtgctctca
    11581 gcgacctcgt gaatatgagg accaacaacc ctgtgcttgg cgctcaggcg caagtgtgtg
    11641 taatttgtcc tccagatcgc agcaatcgcg cccctatctt ggcccgccca cctacttatg
    11701 caggtattcc ccggggtgcc attagtggtt ttgtgggcaa gtggtttgac cgcagtggtt
    11761 agcggggtta caatcagcca agttattaca cccttatttt acagtccaaa accgcagggc
    11821 ggcgtgtggg ggctgacgcg tgcccccact ccacaatttc aaaaaaaaga gtggccactt
    11881 gtctttgttt atgggcccca ttggcgtgga gccccgttta attttcgggg gtgttagaga
    11941 caaccagtgg agtccgctgc tgtcggcgtc cactctcttt ccccttgtta caaatagagt
    12001 gtaacaacat ggttcacctg tcttggtccc tgcctgggac acatcttaat aaccccagta
    12061 tcatattgca ctaggattat gtgttgccca tagccataaa ttcgtgtgag atggacatcc
    12121 agtctttacg gcttgtcccc accccatgga tttctattgt taaagatatt cagaatgttt
    12181 cattcctaca ctagtattta ttgcccaagg ggtttgtgag ggttatattg gtgtcatagc
    12241 acaatgccac cactgaaccc cccgtccaaa ttttattctg ggggcgtcac ctgaaacctt
    12301 gttttcgagc acctcacata caccttactg ttcacaactc agcagttatt ctattagcta
    12361 aacgaaggag aatgaagaag caggcgaaga ttcaggagag ttcactgccc gctccttgat
    12421 cttcagccac tgcccttgtg actaaaatgg ttcactaccc tcgtggaatc ctgaccccat
    12481 gtaaataaaa ccgtgacagc tcatggggtg ggagatatcg ctgttcctta ggaccctttt
    12541 actaacccta attcgatagc atatgcttcc cgttgggtaa catatgctat tgaattaggg
    12601 ttagtctgga tagtatatac tactacccgg gaagcatatg ctacccgttt agggttaaca
    12661 agggggcctt ataaacacta ttgctaatgc cctcttgagg gtccgcttat cggtagctac
    12721 acaggcccct ctgattgacg ttggtgtagc ctcccgtagt cttcctgggc ccctgggagg
    12781 tacatgtccc ccagcattgg tgtaagagct tcagccaaga gttacacata aaggcaatgt
    12841 tgtgttgcag tccacagact gcaaagtctg ctccaggatg aaagccactc agtgttggca
    12901 aatgtgcaca tccatttata aggatgtcaa ctacagtcag agaacccctt tgtgtttggt
    12961 ccccccccgt gtcacatgtg gaacagggcc cagttggcaa gttgtaccaa ccaactgaag
    13021 ggattacatg cactgccccg cgaagaaggg gcagagatgt cgtagtcagg tttagttcgt
    13081 ccggggcggg gatcgatcc
//
