LOCUS       sequence-292298-       13721 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for N-terminal Halo- and c-myc-tagged 
            mGluR3 C127A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13721)
  TITLE     Mammalian expression plasmids for production of metabotropic 
            glutamate receptor type 3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCEP4_HA-Halo-cmyc-GRM3 C127A
FEATURES             Location/Qualifiers
     source          1..13721
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             64..951
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     CDS             964..993
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    3575..3709
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3599..3618)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3653..3672
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3852..3874)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4434..6223
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(8886..8904)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8972..9076
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9077..9145,9146..9937)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9295..9314)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10108..10696
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10597..10616
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        11127..11272
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             11315..12352
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(12350..12369)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    12394..12442
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        13096..13475
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        13476..13679
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     13630..13650
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     13676..13700
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gctagcgcca ccatgaagac catcatcgcc ctgagctaca tcttctgcct ggtgttcgcc
       61 ggagcagaaa tcggtactgg ctttccattc gacccccatt atgtggaagt cctgggcgag
      121 cgcatgcact acgtcgatgt tggtccgcgc gatggcaccc ctgtgctgtt cctgcacggt
      181 aacccgacct cctcctacgt gtggcgcaac atcatcccgc atgttgcacc gacccatcgc
      241 tgcattgctc cagacctgat cggtatgggc aaatccgaca aaccagacct gggttatttc
      301 ttcgacgacc acgtccgctt catggatgcc ttcatcgaag ccctgggtct ggaagaggtc
      361 gtcctggtca ttcacgactg gggctccgct ctgggtttcc actgggccaa gcgcaatcca
      421 gagcgcgtca aaggtattgc atttatggag ttcatccgcc ctatcccgac ctgggacgaa
      481 tggccagaat ttgcccgcga gaccttccag gccttccgca ccaccgacgt cggccgcaag
      541 ctgatcatcg atcagaacgt ttttatcgag ggtacgctgc cgatgggtgt cgtccgcccg
      601 ctgactgaag tcgagatgga ccattaccgc gagccgttcc tgaatcctgt tgaccgcgag
      661 ccactgtggc gcttcccaaa cgagctgcca atcgccggtg agccagcgaa catcgtcgcg
      721 ctggtcgaag aatacatgga ctggctgcac cagtcccctg tcccgaagct gctgttctgg
      781 ggcaccccag gcgttctgat cccaccggcc gaagccgctc gcctggccaa aagcctgcct
      841 aactgcaagg ctgtggacat cggcccgggt ctgaatctgc tgcaagaaga caacccggac
      901 ctgatcggca gcgagatcgc gcgctggctg tcgacgctgg agatttccgg cggaggcggt
      961 ggagaacaaa agcttatttc tgaagaggac ttgggaggat ccaggagaga gattaaaata
     1021 gaaggtgacc ttgttttagg gggcctgttt cctattaacg aaaaaggcac tggaactgaa
     1081 gaatgtgggc gaatcaatga agaccgaggg attcaacgcc tggaagccat gttgtttgct
     1141 attgatgaaa tcaacaaaga tgattacttg ctaccaggag tgaagttggg tgttcacatt
     1201 ttggatacat gttcaaggga tacctatgca ttggagcaat cactggagtt tgtcagggca
     1261 tctttgacaa aagtggatga agctgagtat atggctcctg atggatccta tgccattcaa
     1321 gaaaacatcc cacttctcat tgcaggggtc attggtggct cttatagcag tgtttccata
     1381 caggtggcaa acctgctgcg gctcttccag atccctcaga tcagctacgc atccaccagc
     1441 gccaaactca gtgataagtc gcgctatgat tactttgcca ggaccgtgcc ccccgacttc
     1501 taccaggcca aagccatggc tgagatcttg cgcttcttca actggaccta cgtgtccaca
     1561 gtagcctccg agggtgatta cggggagaca gggatcgagg ccttcgagca ggaagcccgc
     1621 ctgcgcaaca tctgcatcgc tacggcggag aaggtgggcc gctccaacat ccgcaagtcc
     1681 tacgacagcg tgatccgaga actgttgcag aagcccaacg cgcgcgtcgt ggtcctcttc
     1741 atgcgcagcg acgactcgcg ggagctcatt gcagccgcca gccgcgccaa tgcctccttc
     1801 acctgggtgg ccagcgacgg ctggggcgcg caggagagca tcatcaaggg cagcgagcat
     1861 gtggcctacg gcgccatcac cctggagctg gcctcccagc ctgtccgcca gttcgaccgc
     1921 tacttccaga gcctcaaccc ctacaacaac caccgcaacc cctggttccg ggacttctgg
     1981 gagcaaaagt ttcagtgcag cctccagaac aaacgcaacc acaggcgcgt ctgcgacaag
     2041 cacctggcca tcgacagcag caactacgag caagagtcca agatcatgtt tgtggtgaac
     2101 gcggtgtatg ccatggccca cgctttgcac aaaatgcagc gcaccctctg tcccaacact
     2161 accaagcttt gtgatgctat gaagatcctg gatgggaaga agttgtacaa ggattacttg
     2221 ctgaaaatca acttcacggc tccattcaac ccaaataaag atgcagatag catagtcaag
     2281 tttgacactt ttggagatgg aatggggcga tacaacgtgt tcaatttcca aaatgtaggt
     2341 ggaaagtatt cctacttgaa agttggtcac tgggcagaaa ccttatcgct agatgtcaac
     2401 tctatccact ggtcccggaa ctcagtcccc acttcccagt gcagcgaccc ctgtgccccc
     2461 aatgaaatga agaatatgca accaggggat gtctgctgct ggatttgcat cccctgtgaa
     2521 ccgtacgaat acctggctga tgagtttacc tgtatggatt gtgggtctgg acagtggccc
     2581 actgcagacc taactggatg ctatgacctt cctgaggact acatcaggtg ggaagacgcc
     2641 tgggccattg gcccagtcac cattgcctgt ctgggtttta tgtgtacatg catggttgta
     2701 actgttttta tcaagcacaa caacacaccc ttggtcaaag catcgggccg agaactctgc
     2761 tacatcttat tgtttggggt tggcctgtca tactgcatga cattcttctt cattgccaag
     2821 ccatcaccag tcatctgtgc attgcgccga ctcgggctgg ggagttcctt cgctatctgt
     2881 tactcagccc tgctgaccaa gacaaactgc attgcccgca tcttcgatgg ggtcaagaat
     2941 ggcgctcaga ggccaaaatt catcagcccc agttctcagg ttttcatctg cctgggtctg
     3001 atcctggtgc aaattgtgat ggtgtctgtg tggctcatcc tggaggcccc aggcaccagg
     3061 aggtataccc ttgcagagaa gcgggaaaca gtcatcctaa aatgcaatgt caaagattcc
     3121 agcatgttga tctctcttac ctacgatgtg atcctggtga tcttatgcac tgtgtacgcc
     3181 ttcaaaacgc ggaagtgccc agaaaatttc aacgaagcta agttcatagg ttttaccatg
     3241 tacaccacgt gcatcatctg gttggccttc ctccctatat tttatgtgac atcaagtgac
     3301 tacagagtgc agacgacaac catgtgcatc tctgtcagcc tgagtggctt tgtggtcttg
     3361 ggctgtttgt ttgcacccaa ggttcacatc atcctgtttc aaccccagaa gaatgttgtc
     3421 acacacagac tgcacctcaa caggttcagt gtcagtggaa ctgggaccac atactctcag
     3481 tcctctgcaa gcacgtatgt gccaacggtg tgcaatgggc gggaagtcct cgactccacc
     3541 acctcatctc tgtgactcga ggccggcaag gccggatcca gacatgataa gatacattga
     3601 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     3661 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     3721 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     3781 aaacctctac aaatgtggta tggctgatta tgatccggct gcctcgcgcg tttcggtgat
     3841 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     3901 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     3961 gcagccatga ggtcgactct agaggatcga tccccgcccc ggacgaacta aacctgacta
     4021 cgacatctct gccccttctt cgcggggcag tgcatgtaat cccttcagtt ggttggtaca
     4081 acttgccaac tgggccctgt tccacatgtg acacgggggg ggaccaaaca caaaggggtt
     4141 ctctgactgt agttgacatc cttataaatg gatgtgcaca tttgccaaca ctgagtggct
     4201 ttcatcctgg agcagacttt gcagtctgtg gactgcaaca caacattgcc tttatgtgta
     4261 actcttggct gaagctctta caccaatgct gggggacatg tacctcccag gggcccagga
     4321 agactacggg aggctacacc aacgtcaatc agaggggcct gtgtagctac cgataagcgg
     4381 accctcaaga gggcattagc aatagtgttt ataaggcccc cttgttaacc ctaaacgggt
     4441 agcatatgct tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata
     4501 tgttacccaa cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac
     4561 agcgatatct cccaccccat gagctgtcac ggttttattt acatggggtc aggattccac
     4621 gagggtagtg aaccatttta gtcacaaggg cagtggctga agatcaagga gcgggcagtg
     4681 aactctcctg aatcttcgcc tgcttcttca ttctccttcg tttagctaat agaataactg
     4741 ctgagttgtg aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc
     4801 cccagaataa aatttggacg gggggttcag tggtggcatt gtgctatgac accaatataa
     4861 ccctcacaaa ccccttgggc aataaatact agtgtaggaa tgaaacattc tgaatatctt
     4921 taacaataga aatccatggg gtggggacaa gccgtaaaga ctggatgtcc atctcacacg
     4981 aatttatggc tatgggcaac acataatcct agtgcaatat gatactgggg ttattaagat
     5041 gtgtcccagg cagggaccaa gacaggtgaa ccatgttgtt acactctatt tgtaacaagg
     5101 ggaaagagag tggacgccga cagcagcgga ctccactggt tgtctctaac acccccgaaa
     5161 attaaacggg gctccacgcc aatggggccc ataaacaaag acaagtggcc actctttttt
     5221 ttgaaattgt ggagtggggg cacgcgtcag cccccacacg ccgccctgcg gttttggact
     5281 gtaaaataag ggtgtaataa cttggctgat tgtaaccccg ctaaccactg cggtcaaacc
     5341 acttgcccac aaaaccacta atggcacccc ggggaatacc tgcataagta ggtgggcggg
     5401 ccaagatagg ggcgcgattg ctgcgatctg gaggacaaat tacacacact tgcgcctgag
     5461 cgccaagcac agggttgttg gtcctcatat tcacgaggtc gctgagagca cggtgggcta
     5521 atgttgccat gggtagcata tactacccaa atatctggat agcatatgct atcctaatct
     5581 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     5641 atatctgggt agtatatgct atcctaattt atatctgggt agcataggct atcctaatct
     5701 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct
     5761 gtatccgggt agcatatgct atcctaatag agattagggt agtatatgct atcctaattt
     5821 atatctgggt agcatatact acccaaatat ctggatagca tatgctatcc taatctatat
     5881 ctgggtagca tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat
     5941 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat
     6001 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     6061 ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc tcatgcatat
     6121 acagtcagca tatgataccc agtagtagag tgggagtgct atcctttgca tatgccgcca
     6181 cctcccaagg gggcgtgaat tttcgctgct tgtccttttc ctgctggttg ctcccattct
     6241 taggtgaatt taaggaggcc aggctaaagc cgtcgcatgt ctgattgctc accaggtaaa
     6301 tgtcgctaat gttttccaac gcgagaaggt gttgagcgcg gagctgagtg acgtgacaac
     6361 atgggtatgc ccaattgccc catgttggga ggacgaaaat ggtgacaaga cagatggcca
     6421 gaaatacacc aacagcacgc atgatgtcta ctggggattt attctttagt gcgggggaat
     6481 acacggcttt taatacgatt gagggcgtct cctaacaagt tacatcactc ctgcccttcc
     6541 tcaccctcat ctccatcacc tccttcatct ccgtcatctc cgtcatcacc ctccgcggca
     6601 gccccttcca ccataggtgg aaaccaggga ggcaaatcta ctccatcgtc aaagctgcac
     6661 acagtcaccc tgatattgca ggtaggagcg ggctttgtca taacaaggtc cttaatcgca
     6721 tccttcaaaa cctcagcaaa tatatgagtt tgtaaaaaga ccatgaaata acagacaatg
     6781 gactccctta gcgggccagg ttgtgggccg ggtccagggg ccattccaaa ggggagacga
     6841 ctcaatggtg taagacgaca ttgtggaata gcaagggcag ttcctcgcct taggttgtaa
     6901 agggaggtct tactacctcc atatacgaac acaccggcga cccaagttcc ttcgtcggta
     6961 gtcctttcta cgtgactcct agccaggaga gctcttaaac cttctgcaat gttctcaaat
     7021 ttcgggttgg aacctccttg accacgatgc tttccaaacc accctccttt tttgcgcctg
     7081 cctccatcac cctgaccccg gggtccagtg cttgggcctt ctcctgggtc atctgcgggg
     7141 ccctgctcta tcgctcccgg gggcacgtca ggctcaccat ctgggccacc ttcttggtgg
     7201 tattcaaaat aatcggcttc ccctacaggg tggaaaaatg gccttctacc tggagggggc
     7261 ctgcgcggtg gagacccgga tgatgatgac tgactactgg gactcctggg cctcttttct
     7321 ccacgtccac gacctctccc cctggctctt tcacgacttc cccccctggc tctttcacgt
     7381 cctctacccc ggcggcctcc actacctcct cgaccccggc ctccactacc tcctcgaccc
     7441 cggcctccac tgcctcctcg accccggcct ccacctcctg ctcctgcccc tcctgctcct
     7501 gcccctcctc ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc tgcccctcct
     7561 gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctcc tgctcctgcc
     7621 cctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc
     7681 cctccngctc ctgcccctcc tgcccctcct gctcctgccc ctcctgctcc tscccctcct
     7741 gctcctsccc ctcctsctcc tgcccctcct gcccctccts cccctcctcc tgctcctgcc
     7801 cctcctgctc ctgcccctcc tgcccctcct gcccctcctg ctcctgcccc tcctcctgct
     7861 cctgcccctc ctscycctcc tscccctcct cctsctcctg cccctcctgc ccctcctcct
     7921 gctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctgcccctcc tcctsctcct
     7981 gcccctcctc ctcctccysc ysctccthcc cctcckccbc ytncctrccc cwsctcccmc
     8041 rcckkccccw ccwcctmctc cwscccckcc tcctcctccy scscckccys cscctcctsc
     8101 ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct cctgcccctc ctgctcctgc
     8161 ccctcccgct cctgctcctg ctcctgttcc accgtgggtc cctttgcagc caatgcaact
     8221 tggacgtttt tggggtctcc ggacaccatc tctatgtctt ggccctgatc ctgagccgcc
     8281 cggggctcct ggtcttccgc ctcctcgtcc tcgtcctctt ccccgtcctc gtccatggtt
     8341 atcaccccct cttctttgag gtccactgcc gccggagcct tctggtccag atgtgtctcc
     8401 cttctctcct aggccatttc caggtcctgt acctggcccc tcgtcagaca tgattcacac
     8461 taaaagagat caatagacat ctttattaga cgacgctcag tgaatacagg gagtgcagac
     8521 tcctgccccc tccaacagcc cccccaccct catccccttc atggtcgctg tcagacagat
     8581 ccaggtctga aaattcccca tcctccgaac catcctcgtc ctcatcacca attactcgca
     8641 gcccggaaaa ctcccgctga acatcctcaa gatttgcgtc ctgagcctca agccaggcct
     8701 caaattcctc gtcccccttt ttgctggacg gtagggatgg ggattctcgg gacccctcct
     8761 cttcctcttc aaggtcacca gacagagatg ctactggggc aacggaagaa aagctgggtg
     8821 cggcctgtga ggatcagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg
     8881 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     8941 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     9001 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     9061 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     9121 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     9181 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     9241 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     9301 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     9361 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     9421 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     9481 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     9541 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     9601 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga
     9661 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     9721 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     9781 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     9841 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     9901 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     9961 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
    10021 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    10081 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
    10141 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
    10201 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
    10261 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
    10321 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
    10381 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    10441 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
    10501 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
    10561 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    10621 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    10681 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
    10741 gccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc
    10801 gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg
    10861 cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc ccgagatgcg ccgcgtgcgg
    10921 ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca
    10981 gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc
    11041 cgccctgctt catccccgtg gcccgttgct cgcgtttgct ggcggtgtcc ccggaagaaa
    11101 tatatttgca tgtctttagt tctatgatga cacaaacccc gcccagcgtc ttgtcattgg
    11161 cgaattcgaa cacgcagatg cagtcggggc ggcgcggtcc gaggtccact tcgcatatta
    11221 aggtgacgcg tgtggcctcg aacaccgagc gaccctgcag cgacccgctt aacagcgtca
    11281 acagcgtgcc gcagatcccg gggggcaatg agatatgaaa aagcctgaac tcaccgcgac
    11341 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
    11401 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
    11461 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
    11521 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta
    11581 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
    11641 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
    11701 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
    11761 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
    11821 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
    11881 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
    11941 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
    12001 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
    12061 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
    12121 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
    12181 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
    12241 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
    12301 aaaccgacgc cccagcactc gtccggatcg ggagatgggg gaggctaact gaaacacgga
    12361 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
    12421 acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
    12481 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
    12541 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
    12601 gccatagcca ctggccccgt gggttaggga cggggtcccc catggggaat ggtttatggt
    12661 tcgtgggggt tattattttg ggcgttgcgt ggggtcaggt ccacgactgg actgagcaga
    12721 cagacccatg gtttttggat ggcctgggca tggaccgcat gtactggcgc gacacgaaca
    12781 ccgggcgtct gtggctgcca aacacccccg acccccaaaa accaccgcgc ggatttctgg
    12841 cgtgccaagc tagtcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa
    12901 cgttgttgcc attgctgcag gcgcagaact ggtaggtatg gaagatctat acattgaatc
    12961 aatattggca attagccata ttagtcattg gttatatagc ataaatcaat attggctatt
    13021 ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa
    13081 tatgaccgcc atgttgacat tgattattga ctagttatta atagtaatca attacggggt
    13141 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
    13201 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
    13261 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
    13321 acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg
    13381 gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc
    13441 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca
    13501 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
    13561 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg
    13621 ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
    13681 gtttagtgaa ccgtcagatc tctagaagct gggtaccagc t
//
