LOCUS       sequence-292299-       13080 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and T174A
            mutant fused to the C-terminal half of split fluorescent Venus (VC).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13080)
  TITLE     Mammalian expression plasmids for production of metabotropic 
            glutamate receptor type 3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCEP4_HLA-cmyc-GRM3 C127A T174A-VC
FEATURES             Location/Qualifiers
     source          1..13080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     42..66
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      94..103
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             178..207
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2767..3018
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     2955..2976
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3041..3175
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3065..3084)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3119..3138
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3318..3340)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3900..5689
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(8332..8350)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8418..8522
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8523..8591,8592..9383)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8741..8760)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9554..10142
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10043..10062
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10573..10718
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10761..11798
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11796..11815)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11840..11888
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        12542..12921
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(12922..13080,1..45)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     join(13076..13080,1..16)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
       61 cagatctcta gaagctgggt accagctgct agcgccacca tggccgtcat ggcgccccga
      121 accctcgtcc tgctactctc gggggccctg gccctgaccc agacctgggc gggctccgaa
      181 caaaagctta tttctgaaga ggacttggga ggatccagga gagagattaa aatagaaggt
      241 gaccttgttt tagggggcct gtttcctatt aacgaaaaag gcactggaac tgaagaatgt
      301 gggcgaatca atgaagaccg agggattcaa cgcctggaag ccatgttgtt tgctattgat
      361 gaaatcaaca aagatgatta cttgctacca ggagtgaagt tgggtgttca cattttggat
      421 acatgttcaa gggataccta tgcattggag caatcactgg agtttgtcag ggcatctttg
      481 acaaaagtgg atgaagctga gtatatggct cctgatggat cctatgccat tcaagaaaac
      541 atcccacttc tcattgcagg ggtcattggt ggctcttata gcagtgtttc catacaggtg
      601 gcaaacctgc tgcggctctt ccagatccct cagatcagct acgcatccgc cagcgccaaa
      661 ctcagtgata agtcgcgcta tgattacttt gccaggaccg tgccccccga cttctaccag
      721 gccaaagcca tggctgagat cttgcgcttc ttcaactgga cctacgtgtc cacagtagcc
      781 tccgagggtg attacgggga gacagggatc gaggccttcg agcaggaagc ccgcctgcgc
      841 aacatctgca tcgctacggc ggagaaggtg ggccgctcca acatccgcaa gtcctacgac
      901 agcgtgatcc gagaactgtt gcagaagccc aacgcgcgcg tcgtggtcct cttcatgcgc
      961 agcgacgact cgcgggagct cattgcagcc gccagccgcg ccaatgcctc cttcacctgg
     1021 gtggccagcg acggctgggg cgcgcaggag agcatcatca agggcagcga gcatgtggcc
     1081 tacggcgcca tcaccctgga gctggcctcc cagcctgtcc gccagttcga ccgctacttc
     1141 cagagcctca acccctacaa caaccaccgc aacccctggt tccgggactt ctgggagcaa
     1201 aagtttcagt gcagcctcca gaacaaacgc aaccacaggc gcgtctgcga caagcacctg
     1261 gccatcgaca gcagcaacta cgagcaagag tccaagatca tgtttgtggt gaacgcggtg
     1321 tatgccatgg cccacgcttt gcacaaaatg cagcgcaccc tctgtcccaa cactaccaag
     1381 ctttgtgatg ctatgaagat cctggatggg aagaagttgt acaaggatta cttgctgaaa
     1441 atcaacttca cggctccatt caacccaaat aaagatgcag atagcatagt caagtttgac
     1501 acttttggag atggaatggg gcgatacaac gtgttcaatt tccaaaatgt aggtggaaag
     1561 tattcctact tgaaagttgg tcactgggca gaaaccttat cgctagatgt caactctatc
     1621 cactggtccc ggaactcagt ccccacttcc cagtgcagcg acccctgtgc ccccaatgaa
     1681 atgaagaata tgcaaccagg ggatgtctgc tgctggattt gcatcccctg tgaaccgtac
     1741 gaatacctgg ctgatgagtt tacctgtatg gattgtgggt ctggacagtg gcccactgca
     1801 gacctaactg gatgctatga ccttcctgag gactacatca ggtgggaaga cgcctgggcc
     1861 attggcccag tcaccattgc ctgtctgggt tttatgtgta catgcatggt tgtaactgtt
     1921 tttatcaagc acaacaacac acccttggtc aaagcatcgg gccgagaact ctgctacatc
     1981 ttattgtttg gggttggcct gtcatactgc atgacattct tcttcattgc caagccatca
     2041 ccagtcatct gtgcattgcg ccgactcggg ctggggagtt ccttcgctat ctgttactca
     2101 gccctgctga ccaagacaaa ctgcattgcc cgcatcttcg atggggtcaa gaatggcgct
     2161 cagaggccaa aattcatcag ccccagttct caggttttca tctgcctggg tctgatcctg
     2221 gtgcaaattg tgatggtgtc tgtgtggctc atcctggagg ccccaggcac caggaggtat
     2281 acccttgcag agaagcggga aacagtcatc ctaaaatgca atgtcaaaga ttccagcatg
     2341 ttgatctctc ttacctacga tgtgatcctg gtgatcttat gcactgtgta cgccttcaaa
     2401 acgcggaagt gcccagaaaa tttcaacgaa gctaagttca taggttttac catgtacacc
     2461 acgtgcatca tctggttggc cttcctccct atattttatg tgacatcaag tgactacaga
     2521 gtgcagacga caaccatgtg catctctgtc agcctgagtg gctttgtggt cttgggctgt
     2581 ttgtttgcac ccaaggttca catcatcctg tttcaacccc agaagaatgt tgtcacacac
     2641 agactgcacc tcaacaggtt cagtgtcagt ggaactggga ccacatactc tcagtcctct
     2701 gcaagcacgt atgtgccaac ggtgtgcaat gggcgggaag tcctcgactc caccacctca
     2761 tctctggaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag
     2821 gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2881 gtgctgctgc ccgacaacca ctacctgagc taccagtcca agctgagcaa agaccccaac
     2941 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggt
     3001 atggacgagc tgtacaagta actcgaggcc ggcaaggccg gatccagaca tgataagata
     3061 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     3121 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     3181 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     3241 caagtaaaac ctctacaaat gtggtatggc tgattatgat ccggctgcct cgcgcgtttc
     3301 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     3361 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     3421 cggggcgcag ccatgaggtc gactctagag gatcgatccc cgccccggac gaactaaacc
     3481 tgactacgac atctctgccc cttcttcgcg gggcagtgca tgtaatccct tcagttggtt
     3541 ggtacaactt gccaactggg ccctgttcca catgtgacac ggggggggac caaacacaaa
     3601 ggggttctct gactgtagtt gacatcctta taaatggatg tgcacatttg ccaacactga
     3661 gtggctttca tcctggagca gactttgcag tctgtggact gcaacacaac attgccttta
     3721 tgtgtaactc ttggctgaag ctcttacacc aatgctgggg gacatgtacc tcccaggggc
     3781 ccaggaagac tacgggaggc tacaccaacg tcaatcagag gggcctgtgt agctaccgat
     3841 aagcggaccc tcaagagggc attagcaata gtgtttataa ggcccccttg ttaaccctaa
     3901 acgggtagca tatgcttccc gggtagtagt atatactatc cagactaacc ctaattcaat
     3961 agcatatgtt acccaacggg aagcatatgc tatcgaatta gggttagtaa aagggtccta
     4021 aggaacagcg atatctccca ccccatgagc tgtcacggtt ttatttacat ggggtcagga
     4081 ttccacgagg gtagtgaacc attttagtca caagggcagt ggctgaagat caaggagcgg
     4141 gcagtgaact ctcctgaatc ttcgcctgct tcttcattct ccttcgttta gctaatagaa
     4201 taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt
     4261 gacgccccca gaataaaatt tggacggggg gttcagtggt ggcattgtgc tatgacacca
     4321 atataaccct cacaaacccc ttgggcaata aatactagtg taggaatgaa acattctgaa
     4381 tatctttaac aatagaaatc catggggtgg ggacaagccg taaagactgg atgtccatct
     4441 cacacgaatt tatggctatg ggcaacacat aatcctagtg caatatgata ctggggttat
     4501 taagatgtgt cccaggcagg gaccaagaca ggtgaaccat gttgttacac tctatttgta
     4561 acaaggggaa agagagtgga cgccgacagc agcggactcc actggttgtc tctaacaccc
     4621 ccgaaaatta aacggggctc cacgccaatg gggcccataa acaaagacaa gtggccactc
     4681 ttttttttga aattgtggag tgggggcacg cgtcagcccc cacacgccgc cctgcggttt
     4741 tggactgtaa aataagggtg taataacttg gctgattgta accccgctaa ccactgcggt
     4801 caaaccactt gcccacaaaa ccactaatgg caccccgggg aatacctgca taagtaggtg
     4861 ggcgggccaa gataggggcg cgattgctgc gatctggagg acaaattaca cacacttgcg
     4921 cctgagcgcc aagcacaggg ttgttggtcc tcatattcac gaggtcgctg agagcacggt
     4981 gggctaatgt tgccatgggt agcatatact acccaaatat ctggatagca tatgctatcc
     5041 taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
     5101 taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc
     5161 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
     5221 taatctgtat ccgggtagca tatgctatcc taatagagat tagggtagta tatgctatcc
     5281 taatttatat ctgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     5341 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat
     5401 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     5461 ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     5521 ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctcat
     5581 gcatatacag tcagcatatg atacccagta gtagagtggg agtgctatcc tttgcatatg
     5641 ccgccacctc ccaagggggc gtgaattttc gctgcttgtc cttttcctgc tggttgctcc
     5701 cattcttagg tgaatttaag gaggccaggc taaagccgtc gcatgtctga ttgctcacca
     5761 ggtaaatgtc gctaatgttt tccaacgcga gaaggtgttg agcgcggagc tgagtgacgt
     5821 gacaacatgg gtatgcccaa ttgccccatg ttgggaggac gaaaatggtg acaagacaga
     5881 tggccagaaa tacaccaaca gcacgcatga tgtctactgg ggatttattc tttagtgcgg
     5941 gggaatacac ggcttttaat acgattgagg gcgtctccta acaagttaca tcactcctgc
     6001 ccttcctcac cctcatctcc atcacctcct tcatctccgt catctccgtc atcaccctcc
     6061 gcggcagccc cttccaccat aggtggaaac cagggaggca aatctactcc atcgtcaaag
     6121 ctgcacacag tcaccctgat attgcaggta ggagcgggct ttgtcataac aaggtcctta
     6181 atcgcatcct tcaaaacctc agcaaatata tgagtttgta aaaagaccat gaaataacag
     6241 acaatggact cccttagcgg gccaggttgt gggccgggtc caggggccat tccaaagggg
     6301 agacgactca atggtgtaag acgacattgt ggaatagcaa gggcagttcc tcgccttagg
     6361 ttgtaaaggg aggtcttact acctccatat acgaacacac cggcgaccca agttccttcg
     6421 tcggtagtcc tttctacgtg actcctagcc aggagagctc ttaaaccttc tgcaatgttc
     6481 tcaaatttcg ggttggaacc tccttgacca cgatgctttc caaaccaccc tccttttttg
     6541 cgcctgcctc catcaccctg accccggggt ccagtgcttg ggccttctcc tgggtcatct
     6601 gcggggccct gctctatcgc tcccgggggc acgtcaggct caccatctgg gccaccttct
     6661 tggtggtatt caaaataatc ggcttcccct acagggtgga aaaatggcct tctacctgga
     6721 gggggcctgc gcggtggaga cccggatgat gatgactgac tactgggact cctgggcctc
     6781 ttttctccac gtccacgacc tctccccctg gctctttcac gacttccccc cctggctctt
     6841 tcacgtcctc taccccggcg gcctccacta cctcctcgac cccggcctcc actacctcct
     6901 cgaccccggc ctccactgcc tcctcgaccc cggcctccac ctcctgctcc tgcccctcct
     6961 gctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc
     7021 cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc tcctcctgct
     7081 cctgcccctc ctgcccctcc tcctgctcct gcccctcctg cccctcctgc tcctgcccct
     7141 cctgcccctc ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcg cckccmcctm
     7201 ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccyt cctdctccts
     7261 cycctcctmc cccscctgcy ccwcctgctc ctscycctcc tcctgctcct scccctcctg
     7321 ctsctcctgc ccctcctcct gctcctsccc ctcctgcccc tcctcctgct cctgcccctc
     7381 ctcctgctcc tgcccctcct gcycctcctg cccctcctcc tgctcctgcc cctcctgccc
     7441 ctcctcctsc tcctgccyct cctcctsctc ctsccsctcc trcccctcct gcycckcctc
     7501 ctsctcctgc ycctcctcct sctcctsccc ctcctgcccc tcctgcccct cctgcccctc
     7561 ctcctgctcc tgcccctcct cctgctcctg cccctcctgc tcctgcccct cccgctcctg
     7621 ctcctgctcc tgttccaccg tgggtccctt tgcagccaat gcaacttgga cgtttttggg
     7681 gtctccggac accatctcta tgtcttggcc ctgatcctga gccgcccggg gctcctggtc
     7741 ttccgcctcc tcgtcctcgt cctcttcccc gtcctcgtcc atggttatca ccccctcttc
     7801 tttgaggtcc actgccgccg gagccttctg gtccagatgt gtctcccttc tctcctaggc
     7861 catttccagg tcctgtacct ggcccctcgt cagacatgat tcacactaaa agagatcaat
     7921 agacatcttt attagacgac gctcagtgaa tacagggagt gcagactcct gccccctcca
     7981 acagcccccc caccctcatc cccttcatgg tcgctgtcag acagatccag gtctgaaaat
     8041 tccccatcct ccgaaccatc ctcgtcctca tcaccaatta ctcgcagccc ggaaaactcc
     8101 cgctgaacat cctcaagatt tgcgtcctga gcctcaagcc aggcctcaaa ttcctcgtcc
     8161 ccctttttgc tggacggtag ggatggggat tctcgggacc cctcctcttc ctcttcaagg
     8221 tcaccagaca gagatgctac tggggcaacg gaagaaaagc tgggtgcggc ctgtgaggat
     8281 cagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga
     8341 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     8401 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     8461 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     8521 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     8581 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     8641 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     8701 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     8761 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     8821 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     8881 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     8941 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     9001 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     9061 tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     9121 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     9181 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     9241 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     9301 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     9361 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     9421 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     9481 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     9541 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     9601 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     9661 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     9721 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     9781 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     9841 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     9901 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     9961 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    10021 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    10081 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    10141 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tgaagctgtc
    10201 cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc
    10261 cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca
    10321 gcaagacgta gcccagcgcg tcggccccga gatgcgccgc gtgcggctgc tggagatggc
    10381 ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa
    10441 ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ctgcttcatc
    10501 cccgtggccc gttgctcgcg tttgctggcg gtgtccccgg aagaaatata tttgcatgtc
    10561 tttagttcta tgatgacaca aaccccgccc agcgtcttgt cattggcgaa ttcgaacacg
    10621 cagatgcagt cggggcggcg cggtccgagg tccacttcgc atattaaggt gacgcgtgtg
    10681 gcctcgaaca ccgagcgacc ctgcagcgac ccgcttaaca gcgtcaacag cgtgccgcag
    10741 atcccggggg gcaatgagat atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
    10801 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
    10861 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
    10921 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
    10981 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
    11041 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
    11101 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
    11161 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
    11221 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
    11281 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
    11341 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
    11401 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
    11461 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
    11521 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
    11581 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
    11641 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
    11701 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
    11761 gcactcgtcc ggatcgggag atgggggagg ctaactgaaa cacggaagga gacaataccg
    11821 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg gtgttgggtc
    11881 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
    11941 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
    12001 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagccactgg
    12061 ccccgtgggt tagggacggg gtcccccatg gggaatggtt tatggttcgt gggggttatt
    12121 attttgggcg ttgcgtgggg tcaggtccac gactggactg agcagacaga cccatggttt
    12181 ttggatggcc tgggcatgga ccgcatgtac tggcgcgaca cgaacaccgg gcgtctgtgg
    12241 ctgccaaaca cccccgaccc ccaaaaacca ccgcgcggat ttctggcgtg ccaagctagt
    12301 cgaccaattc tcatgtttga cagcttatca tcgcagatcc gggcaacgtt gttgccattg
    12361 ctgcaggcgc agaactggta ggtatggaag atctatacat tgaatcaata ttggcaatta
    12421 gccatattag tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg
    12481 ttgtatctat atcataatat gtacatttat attggctcat gtccaatatg accgccatgt
    12541 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
    12601 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
    12661 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
    12721 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
    12781 caagtgtatc atatgccaag tccgccccct attgacgtca atgacggtaa atggcccgcc
    12841 tggcattatg cccagtacat gaccttacgg gactttccta cttggcagta catctacgta
    12901 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag
    12961 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
    13021 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa
//
