LOCUS       sequence-29231-e         610 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-FLJ40852
FEATURES             Location/Qualifiers
     source          1..610
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..109
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(355..454)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(471..490)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(472..490)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(495..511)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(495..511)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agctcgggcc caataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
       61 gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagttggcac catggggaaa
      121 gcgcgaatgc agtcccccac tacaacaaat tatgcagtcg agttttccac atttgggaat
      181 atcacagggg tcagcacatc cggagtgcaa tcgatgagcc ccacgctggg aaaaaccacc
      241 ttcgtgatca tggtatcttc cctgccagag agaaaccaat cagcttcatt ttcaacttct
      301 tctatgactc cagaggattc actttgcatc ttgtgacaaa tgaatatatt accttgccaa
      361 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
      421 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg
      481 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
      541 gatgttacat tgcacaagat aaaaatatat catcatgcct cctctagacc agccaggaca
      601 gaaatgcctc
//
