LOCUS       sequence-292356-       11605 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  parental, all-in-one CRISPRi vector for B. burgdorferi, parental for
            gRNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11605)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 11605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9H(RBSmut)_arr2
FEATURES             Location/Qualifiers
     source          1..11605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(5371..5385,5386..5391,5392..5408,5409..5415)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    5391..5407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5423..5439
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             join(5476..5502,5503..5505,5506..7992,7993..7995,
                     7996..9579)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9809..10828
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     rep_origin      10951..11539
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11440..11459
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcactag atccccggca agttcatcct tagggtttcc atatctctta aaaagtcaat
       61 ttctgcttga ttaatttctt gtggattatt gtttttgcgg tttttcattt tttatctcct
      121 tgagtattga attttttctt atatataaat atatagcaaa aactattttt gccaactttt
      181 ttacaaaaaa ttttttatta aaacacttag ggctttgcta aattctcttt taaaagaact
      241 tagtaaagcc ctatggattt aagaactgat tatacttacg tagtaaaaat actacagata
      301 ttaatagtat agaccatatc aaaatcataa tcaatttata tttatctaaa attgtagtcc
      361 atacaaaata caaaaaaatt aggtttttct aaattctatt ttaaaagaac ttagtaacac
      421 ctaatctcta aattacttac aaaattctaa aattagattt ttagttcttc atatttcttt
      481 aaaagttttt taagaaaatc tttttgattt tcaaaaattt catgcatcat aaaactagta
      541 aatttggatt tttttttgta aaaatcataa ctttcttggg ttttaagttg aaatcttaat
      601 ggttttattg gattttgcct agattttttt aacgccggac tttctttatc ttttataaaa
      661 ttataagaat ttttaatacc attttctatt aaataatctt cttttataag cccctcttgc
      721 agtgcagttg ctaatcttaa atacttatac gcttgagttt ttgctacatc aaagtctttt
      781 ataaaatcaa gaaaagtttt ataaccatca aatttataat attgattatc ttttatttct
      841 tttaaaattt taatattttc caccctgtgg aatatttctt ttttgaaatt gtcatttaac
      901 ttttccttta gtgtcttgta ataagaaatt ttactttcat taatcattaa attttgttca
      961 acataagtat ctatcttttt tgttaaaact ttaacaacac gttttttttc cataaaaatt
     1021 ttcctttatt tttgcaacat tttccttaat tgatataatt gttactattt tagatttttt
     1081 aatatccact taagaattaa tccctcacta gaatattttt tattatagaa ttcatataca
     1141 tgtttttcta aatctataaa tttatcaaca attgctttat tatagggtgt atctatcttt
     1201 tcttttctta ataatcgtct catattcttt aaataacaac aaacactgcc ggttttaaac
     1261 ctaaattcta tgtaatatat ttttgaaaaa gtatatacat tttcaacccc gtttatttca
     1321 tactttacaa aaacattatt tattggtttt ctataaccat agtaaatgcc taaaaatttg
     1381 tccccgtctt tcataggata tagataaagt ttttgaatct ctgatttatc taataatttt
     1441 tcaaaaaaaa ttatacaccg cttttgtttg ttactaacta ctctatgcaa tgcctttaat
     1501 atttttgtgt gatatatatt tttatcacct tttttttctt tcagcaggaa gatactttct
     1561 tttggaaatt taattttttg ttcatttatt cttgcagcct taagtccgat tttcatattt
     1621 aatccttata tagcctttct atttaattct tttaaactca ataaactttt ttggtttttc
     1681 attagttcta ataattcgca ataatatgtg caaaacactt tattgtattc tattttcttt
     1741 tggttattca aatattcttt tataattggc tttagaagtt caatatttac ttcttttttt
     1801 agttgttcaa taaggatatt gaaaatgttt gtttttaaat cttgataatt ttgttgagaa
     1861 ttttcttttt ttctttcaat tgacttctct aatttacgct ttatattgtt taaatcgcta
     1921 tatttatgac tttcaataat aaaatgaggc ttaaatttgt aattttcgta tattttttgg
     1981 aaacttgttt ctaattgttt agaattatat tcattttttt caaattcttt ttgagtgttg
     2041 catagaattt cctttaattt ttcttgtttt tctttcatac aagatttagt aaaatgtata
     2101 tttttatttt ttagtaattt aatttctatt atttttatga ttttaagtat tttgattata
     2161 ttatctttat caatatttaa attgaataaa attgtaagaa aaattgaaag aattttttta
     2221 cataaaaagt tacatttatt gaagtaattt cttacttgat acttttctat ttcattaatc
     2281 tttctttctt cttttatatt atttttatta tttaaacact cctttaaatc tacactccca
     2341 tttttagtaa atctatcttt aaggcccctt gaaactctat tttgaaatct taaatctttt
     2401 ttttcttgaa agtatttata gattttttgg taacattctt tttttgaaaa attaagttga
     2461 tagtatattt cggtccccaa atttacccct aagtgtttgt gataattagt tgtgacttta
     2521 aattcttttt ctaatttgta aaggtaattt tgcagtgttt ttagtttaac gggtgtttga
     2581 ctatttcttt taaggttttc attaaagtaa tagagtatgt ttttttgtgt gtatttctta
     2641 tatgttttat ttatgtacgc tagtgttgag ataagaacta ctaatttata ctgtaattta
     2701 ttgtagcaag ttgtggtttt actggttttg tttatattag ataagttttc catttatagc
     2761 tccttgaata ccggattttt tatctataaa gtatatatat cacaattttg atttaaaagt
     2821 aaattctttt tttaaaaata caaaaatttt ttataaatta tatagaccat gatattgtat
     2881 taatactaat tgattttaat ttaaggttga actaaaataa acatagtttt gtagaaaata
     2941 atttttcatt attttgctac ttgatattgg atcgtaaaaa tattagggct ttacaaagtt
     3001 cttttaaaag agaatttagt aaagccctaa gtcttttaat aaaaaatttt ttgtaaaaaa
     3061 gttggcaaaa atagtttttg ctatatattt atatataaga aaaaattcaa tactcaagga
     3121 gataaaaaat ggaaaactta aacaataatc cacaaaaaat taatcaaata aataagcaaa
     3181 ctaattttat tggttttgaa aattttatag aacttgataa tcaagttaat caaactaaag
     3241 aaaaaagtaa attaagtaag ataggtaaga aattacccgg aataagcagt caagtctagt
     3301 ctagtgcggc cgcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
     3361 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     3421 tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatgagga ggtaacgtca
     3481 tggtaaaaga ttggattcct atttctcatg ataattataa acaagtacaa ggaccatttt
     3541 atcatggaac taaagctaat ttagcaattg gtgatttact aactacagga tttatttctc
     3601 attttgaaga tggtcgaatt cttaaacata tttatttttc agctttaatg gaaccagcag
     3661 tttggggagc tgaacttgct atgtcactat ctggtcttga aggtcgtggt tatatatata
     3721 tagttgaacc aacaggacca tttgaagatg atccaaatct tacaaataaa aaatttcctg
     3781 gtaatccaac acaatcttat agaacttgtg aacctttaag aattgttggt gttgttgaag
     3841 attgggaagg acatcctgtt gaattaataa gaggaatgtt agattcatta gaagatttaa
     3901 aacgtcgtgg tttacatgtt attgaagatt aacctggcgc gccaaataat gcccgtgtcc
     3961 ctgttgaata gggacacggt tttttaacaa tttttttatt cctaggcctt agtggccggt
     4021 acccggggat ccttattact ggccgctttc tagcctggat acttgtctgg ctagttgcat
     4081 tagagagtca gctagagccc tagggctagc tgtttgtgta ttcggagcca gtgtagtttt
     4141 tctttttact aacgatacgg gtaataattg attgcctttt acagcctgac cttgagacaa
     4201 ttgcaaaagt ctgtccacag aggtttgacc taataatcta aaatcttgtt tgatagtagt
     4261 aagtggaggt atgtagcaac tagaatcttc tgtatcatca taacccacaa ctgaaatatc
     4321 agccccaact ctaagtccac tttcagtaat agctcgcata gctcctaaag ccatttgatc
     4381 attagcaact aacatagcag tagggacaat gccttcattt aacatttgca ttgtttgctg
     4441 aaatcctgac atagcagacc aatctccttc tctttctgct attggttgta tttgatttct
     4501 tgttaaatac ttgtgccatc ctgctaatct caatctggca cttactgaac ttaagggacc
     4561 tgcaagtagt gcaatttgtt gatggcctaa tgcaacaagg tgttcaactc ctaaccttgt
     4621 tccatcttcg tgagaaaaaa ttatagagtt tattggggtt tggtccgaca catccaaaaa
     4681 caatgctgga acattagtac atgctgcttc aactgcaatc gcatcttggt catcaagagg
     4741 ataattaatt ataagtccac taacacgttg agctaataaa ttatgtactg ctgccttaca
     4801 agcctctaca cctgatctct caaccattga tacaacaacg cttgcaccaa gttgatcagc
     4861 tctcgattta attgcggcta caatttgact aggcgcatgt aatgcaagtg atgatgtggc
     4921 aactccaatt aaaagtgatt gtttccccgc aagttgctga gcaacgcgat ttggtatata
     4981 attcaactct gccatagctg cttcaacctt ctctcttgtt tttgcagata catgacttgc
     5041 ttggttcaca actctcgaaa ccgtttggta gctaaccccg gcatattcag caacatcata
     5101 caaagtaaca ggtttcatgg atcattcctc catgataaaa tttaaatttc tgactttggc
     5161 aaatcctttt gcctgttaaa aagaatagca caaaaattaa aaaaaaagaa acgcaaatag
     5221 caatagcatt aaaaataatt attattattt atttctttga ttaaaatcaa aagcctttaa
     5281 tgtattttct tgatctttca tgcgcttaac ggtaaaatcc aaggtattaa gttcaccttc
     5341 cacttttctc tctctatctt ctctagaaaa tcataaaaaa tttatttgct ttgtgagcgg
     5401 ataacaatta taatagattc aattgtgagc ggataacaat ttcacacaga attcattaaa
     5461 gaagagaaat tacatatgga taagaaatac tcaataggct tagctatcgg cacaaatagc
     5521 gtcggatggg cggtgatcac tgatgaatat aaggttccgt ctaaaaagtt caaggttctg
     5581 ggaaatacag accgccacag tatcaaaaaa aatcttatag gggctctttt atttgacagt
     5641 ggagagacag cggaagcgac tcgtctcaaa cggacagctc gtagaaggta tacacgtcgg
     5701 aagaatcgta tttgttatct acaggagatt ttttcaaatg agatggcgaa agtagatgat
     5761 agtttctttc atcgacttga agagtctttt ttggtggaag aagacaagaa gcatgaacgt
     5821 catcctattt ttggaaatat agtagatgaa gttgcttatc atgagaaata tccaactatc
     5881 tatcatctgc gaaaaaaatt ggtagattct actgataaag cggatttgcg cttaatctat
     5941 ttggccttag cgcatatgat taagtttcgt ggtcattttt tgattgaggg agatttaaat
     6001 cctgataata gtgatgtgga caaactattt atccagttgg tacaaaccta caatcaatta
     6061 tttgaagaaa accctattaa cgcaagtgga gtagatgcta aagcgattct ttctgcacga
     6121 ttgagtaaat caagacgatt agaaaatctc attgctcagc tccccggtga gaagaaaaat
     6181 ggcttatttg ggaatctcat tgctttgtca ttgggtttga cccctaattt taaatcaaat
     6241 tttgatttgg cagaagatgc taaattacag ctttcaaaag atacttacga tgatgattta
     6301 gataatttat tggcgcaaat tggagatcaa tatgctgatt tgtttttggc agctaagaat
     6361 ttatcagatg ctattttact ttcagatatc ctaagagtaa atactgaaat aactaaggct
     6421 cccctatcag cttcaatgat taaacgctac gatgaacatc atcaagactt gactctttta
     6481 aaagctttag ttcgacaaca acttccagaa aagtataaag aaatcttttt tgatcaatca
     6541 aaaaacggat atgcaggtta tattgatggg ggagctagcc aagaagaatt ttataaattt
     6601 atcaaaccaa ttttagaaaa aatggatggt actgaggaat tattggtgaa actaaatcgt
     6661 gaagatttgc tgcgcaagca acggaccttt gacaacggct ctattcccca tcaaattcac
     6721 ttgggtgagc tgcatgctat tttgagaaga caagaagact tttatccatt tttaaaagac
     6781 aatcgtgaga agattgaaaa aatcttgact tttcgaattc cttattatgt tggtccattg
     6841 gcgcgtggca atagtcgttt tgcatggatg actcggaagt ctgaagaaac aattacccca
     6901 tggaattttg aagaagttgt cgataaaggt gcttcagctc aatcatttat tgaacgcatg
     6961 acaaactttg ataaaaatct tccaaatgaa aaagtactac caaaacatag tttgctttat
     7021 gagtatttta cggtttataa cgaattgaca aaggtcaaat atgttactga aggaatgcga
     7081 aaaccagcat ttctttcagg tgaacagaag aaagccattg ttgatttact cttcaaaaca
     7141 aatcgaaaag taaccgttaa gcaattaaaa gaagattatt tcaaaaaaat agaatgtttt
     7201 gatagtgttg aaatttcagg agttgaagat agatttaatg cttcattagg tacctaccat
     7261 gatttgctaa aaattattaa agataaagat tttttggata atgaagaaaa tgaagatatc
     7321 ttagaggata ttgttttaac attgacctta tttgaagata gggagatgat tgaggaaaga
     7381 cttaaaacat atgctcacct ctttgatgat aaggtgatga aacagcttaa acgtcgccgt
     7441 tatactggtt ggggacgttt gtctcgaaaa ttgattaatg gtattaggga taagcaatct
     7501 ggcaaaacaa tattagattt tttgaaatca gatggttttg ccaatcgcaa ttttatgcag
     7561 ctgatccatg atgatagttt gacatttaaa gaagacattc aaaaagcaca agtgtctgga
     7621 caaggcgata gtttacatga acatattgca aatttagctg gtagccctgc tattaaaaaa
     7681 ggtattttac agactgtaaa agttgttgat gaattggtca aagtaatggg gcggcataag
     7741 ccagaaaata tcgttattga aatggcacgt gaaaatcaga caactcaaaa gggccagaaa
     7801 aattcgcgag agcgtatgaa acgaatcgaa gaaggtatca aagaattagg aagtcagatt
     7861 cttaaagagc atcctgttga aaatactcaa ttgcaaaatg aaaagctcta tctctattat
     7921 ctccaaaatg gaagagacat gtatgtggac caagaattag atattaatcg tttaagtgat
     7981 tatgatgtcg atgccattgt tccacaaagt ttccttaaag acgattcaat agacaataag
     8041 gtcttaacgc gttctgataa aaatcgtggt aaatcggata acgttccaag tgaagaagta
     8101 gtcaaaaaga tgaaaaacta ttggagacaa cttctaaacg ccaagttaat cactcaacgt
     8161 aagtttgata atttaacgaa agctgaacgt ggaggtttga gtgaacttga taaagctggt
     8221 tttatcaaac gccaattggt tgaaactcgc caaatcacta agcatgtggc acaaattttg
     8281 gatagtcgca tgaatactaa atacgatgaa aatgataaac ttattcgaga ggttaaagtg
     8341 attaccttaa aatctaaatt agtttctgac ttccgaaaag atttccaatt ctataaagta
     8401 cgtgagatta acaattacca tcatgcccat gatgcgtatc taaatgccgt cgttggaact
     8461 gctttgatta agaaatatcc aaaacttgaa tcggagtttg tctatggtga ttataaagtt
     8521 tatgatgttc gtaaaatgat tgctaagtct gagcaagaaa taggcaaagc aaccgcaaaa
     8581 tatttctttt actctaatat catgaacttc ttcaaaacag aaattacact tgcaaatgga
     8641 gagattcgca aacgccctct aatcgaaact aatggggaaa ctggagaaat tgtctgggat
     8701 aaagggcgag attttgccac agtgcgcaaa gtattgtcca tgccccaagt caatattgtc
     8761 aagaaaacag aagtacagac aggcggattc tccaaggagt caattttacc aaaaagaaat
     8821 tcggacaagc ttattgctcg taaaaaagac tgggatccaa aaaaatatgg tggttttgat
     8881 agtccaacgg tagcttattc agtcctagtg gttgctaagg tggaaaaagg gaaatcgaag
     8941 aagttaaaat ccgttaaaga gttactaggg atcacaatta tggaaagaag ttcctttgaa
     9001 aaaaatccga ttgacttttt agaagctaaa ggatataagg aagttaaaaa agacttaatc
     9061 attaaactac ctaaatatag tctttttgag ttagaaaacg gtcgtaaacg gatgctggct
     9121 agtgccggag aattacaaaa aggaaatgag ctggctctgc caagcaaata tgtgaatttt
     9181 ttatatttag ctagtcatta tgaaaagttg aagggtagtc cagaagataa cgaacaaaaa
     9241 caattgtttg tggagcagca taagcattat ttagatgaga ttattgagca aatcagtgaa
     9301 ttttctaagc gtgttatttt agcagatgcc aatttagata aagttcttag tgcatataac
     9361 aaacatagag acaaaccaat acgtgaacaa gcagaaaata ttattcattt atttacgttg
     9421 acgaatcttg gagctcccgc tgcttttaaa tattttgata caacaattga tcgtaaacga
     9481 tatacgtcta caaaagaagt tttagatgcc actcttatcc atcaatccat cactggtctt
     9541 tatgaaacac gcattgattt gagtcagcta ggaggtgaca cgcgtttgga gcaagtgaag
     9601 ttctggaggg ataaaaatta ataaaagctt gatcagatcc cagctttttt ttgaagtgcc
     9661 tggcagtaag ttgaaaaaat ttgaaataaa cttggactat gttaataaaa aaatacaaat
     9721 taacgaacaa aaagatttga attattatat aatttagtta aaagcaattt taaatgaggg
     9781 aggtttccat catatggata gaagtggtaa acctgaatta actgctacta gtgttgaaaa
     9841 atttttaatt gaaaaatttg atagtgttag tgatttaatg caattaagtg aaggtgaaga
     9901 aagtagagct tttagttttg atgttggtgg tagaggttat gttttaagag ttaatagttg
     9961 tgctgatggt ttttataaag atagatatgt ttatagacat tttgctagtg ctgctttacc
    10021 tattcctgaa gttttagata ttggtgaatt tagtgaaagt ttaacttatt gtattagtag
    10081 aagagcacaa ggtgttactt tacaagattt acctgaaact gaattacctg ctgttttaca
    10141 acctgttgct gaagctatgg atgctattgc ggcggctgat ctcagtcaaa cttcgggttt
    10201 tggtcctttt ggtcctcaag gtattggtca atatactact tggagagatt ttatttgtgc
    10261 tattgctgat cctcatgttt atcattggca aactgttatg gatgatactg ttagtgctag
    10321 tgttgctcaa gcattagatg aattaatgtt atgggctgaa gattgtcctg aagttagaca
    10381 tttagttcat gctgattttg gtagtaataa tgttttaact gataatggta gaattactgc
    10441 tgttattgat tggagtgaag ctatgtttgg tgatagtcaa tatgaagttg ctaatatttt
    10501 tttttggaga ccttggttag cttgtatgga acaacaaact agatattttg aaagaagaca
    10561 tcctgaatta gctggtagtc ctagattaag agcttatatg ttaagaattg gtttagatca
    10621 attatatcaa agtttagttg atggtaattt tgatgatgct gcttgggctc aaggtagatg
    10681 tgatgctatt gttagaagtg gtgctggtac tgttggtaga actcaaattg ctagaagaag
    10741 tgctgctgtt tggactgatg gttgtgttga agttttagct gatagtggta atagaagacc
    10801 tagtactaga cctagagcta aagaataatg acgtcgccgg agcggtcgag ttctggaccg
    10861 accggctcgg gttctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
    10921 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    10981 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    11041 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
    11101 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    11161 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    11221 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    11281 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    11341 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    11401 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    11461 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    11521 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tgggcttttg
    11581 ctggcctttt gctcacatgt tcttt
//
