LOCUS       sequence-292389-       11055 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  all-in-one CRISPRi vector for targeting B. burgdorferi ftsI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11055)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 11055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S_P0526-sgRNAftsI
FEATURES             Location/Qualifiers
     source          1..11055
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        3549..3624
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(5067..5081,5082..5087,5088..5104,5105..5111)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    5087..5103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5119..5135
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5154..5165
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(5172..5198,5199..5201,5202..7688,7689..7691,
                     7692..9275)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9487..10278
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      10401..10989
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10890..10909
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcactag atccccggca agttcatcct tagggtttcc atatctctta aaaagtcaat
       61 ttctgcttga ttaatttctt gtggattatt gtttttgcgg tttttcattt tttatctcct
      121 tgagtattga attttttctt atatataaat atatagcaaa aactattttt gccaactttt
      181 ttacaaaaaa ttttttatta aaacacttag ggctttgcta aattctcttt taaaagaact
      241 tagtaaagcc ctatggattt aagaactgat tatacttacg tagtaaaaat actacagata
      301 ttaatagtat agaccatatc aaaatcataa tcaatttata tttatctaaa attgtagtcc
      361 atacaaaata caaaaaaatt aggtttttct aaattctatt ttaaaagaac ttagtaacac
      421 ctaatctcta aattacttac aaaattctaa aattagattt ttagttcttc atatttcttt
      481 aaaagttttt taagaaaatc tttttgattt tcaaaaattt catgcatcat aaaactagta
      541 aatttggatt tttttttgta aaaatcataa ctttcttggg ttttaagttg aaatcttaat
      601 ggttttattg gattttgcct agattttttt aacgccggac tttctttatc ttttataaaa
      661 ttataagaat ttttaatacc attttctatt aaataatctt cttttataag cccctcttgc
      721 agtgcagttg ctaatcttaa atacttatac gcttgagttt ttgctacatc aaagtctttt
      781 ataaaatcaa gaaaagtttt ataaccatca aatttataat attgattatc ttttatttct
      841 tttaaaattt taatattttc caccctgtgg aatatttctt ttttgaaatt gtcatttaac
      901 ttttccttta gtgtcttgta ataagaaatt ttactttcat taatcattaa attttgttca
      961 acataagtat ctatcttttt tgttaaaact ttaacaacac gttttttttc cataaaaatt
     1021 ttcctttatt tttgcaacat tttccttaat tgatataatt gttactattt tagatttttt
     1081 aatatccact taagaattaa tccctcacta gaatattttt tattatagaa ttcatataca
     1141 tgtttttcta aatctataaa tttatcaaca attgctttat tatagggtgt atctatcttt
     1201 tcttttctta ataatcgtct catattcttt aaataacaac aaacactgcc ggttttaaac
     1261 ctaaattcta tgtaatatat ttttgaaaaa gtatatacat tttcaacccc gtttatttca
     1321 tactttacaa aaacattatt tattggtttt ctataaccat agtaaatgcc taaaaatttg
     1381 tccccgtctt tcataggata tagataaagt ttttgaatct ctgatttatc taataatttt
     1441 tcaaaaaaaa ttatacaccg cttttgtttg ttactaacta ctctatgcaa tgcctttaat
     1501 atttttgtgt gatatatatt tttatcacct tttttttctt tcagcaggaa gatactttct
     1561 tttggaaatt taattttttg ttcatttatt cttgcagcct taagtccgat tttcatattt
     1621 aatccttata tagcctttct atttaattct tttaaactca ataaactttt ttggtttttc
     1681 attagttcta ataattcgca ataatatgtg caaaacactt tattgtattc tattttcttt
     1741 tggttattca aatattcttt tataattggc tttagaagtt caatatttac ttcttttttt
     1801 agttgttcaa taaggatatt gaaaatgttt gtttttaaat cttgataatt ttgttgagaa
     1861 ttttcttttt ttctttcaat tgacttctct aatttacgct ttatattgtt taaatcgcta
     1921 tatttatgac tttcaataat aaaatgaggc ttaaatttgt aattttcgta tattttttgg
     1981 aaacttgttt ctaattgttt agaattatat tcattttttt caaattcttt ttgagtgttg
     2041 catagaattt cctttaattt ttcttgtttt tctttcatac aagatttagt aaaatgtata
     2101 tttttatttt ttagtaattt aatttctatt atttttatga ttttaagtat tttgattata
     2161 ttatctttat caatatttaa attgaataaa attgtaagaa aaattgaaag aattttttta
     2221 cataaaaagt tacatttatt gaagtaattt cttacttgat acttttctat ttcattaatc
     2281 tttctttctt cttttatatt atttttatta tttaaacact cctttaaatc tacactccca
     2341 tttttagtaa atctatcttt aaggcccctt gaaactctat tttgaaatct taaatctttt
     2401 ttttcttgaa agtatttata gattttttgg taacattctt tttttgaaaa attaagttga
     2461 tagtatattt cggtccccaa atttacccct aagtgtttgt gataattagt tgtgacttta
     2521 aattcttttt ctaatttgta aaggtaattt tgcagtgttt ttagtttaac gggtgtttga
     2581 ctatttcttt taaggttttc attaaagtaa tagagtatgt ttttttgtgt gtatttctta
     2641 tatgttttat ttatgtacgc tagtgttgag ataagaacta ctaatttata ctgtaattta
     2701 ttgtagcaag ttgtggtttt actggttttg tttatattag ataagttttc catttatagc
     2761 tccttgaata ccggattttt tatctataaa gtatatatat cacaattttg atttaaaagt
     2821 aaattctttt tttaaaaata caaaaatttt ttataaatta tatagaccat gatattgtat
     2881 taatactaat tgattttaat ttaaggttga actaaaataa acatagtttt gtagaaaata
     2941 atttttcatt attttgctac ttgatattgg atcgtaaaaa tattagggct ttacaaagtt
     3001 cttttaaaag agaatttagt aaagccctaa gtcttttaat aaaaaatttt ttgtaaaaaa
     3061 gttggcaaaa atagtttttg ctatatattt atatataaga aaaaattcaa tactcaagga
     3121 gataaaaaat ggaaaactta aacaataatc cacaaaaaat taatcaaata aataagcaaa
     3181 ctaattttat tggttttgaa aattttatag aacttgataa tcaagttaat caaactaaag
     3241 aaaaaagtaa attaagtaag ataggtaaga aattacccgg aataagcagt caagtctagt
     3301 ctagtgcggc cggaaacagc tatgacatga ttacgaattc gagctccttg ttttcaatga
     3361 taggtttttt aggtagagac ttgtaagggt ttgttcctgg aatataattt ttaggaattt
     3421 tcatatattt tactcctatt taactagcta acctataatt ctattatttt ttttgttttt
     3481 atgcaatcaa tattgatatt ttgatttttt atgattagaa tcatcatgag tgttattaag
     3541 cattctttgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt
     3601 gaaaaagtgg caccgagtcg gtgctttttt tggcgcgcca aataatgccc gtgtccctgt
     3661 tgaataggga cacggttttt taacaatttt tttattccta ggccttagtg gccggtaccc
     3721 ggggatcctt attactggcc gctttctagc ctggatactt gtctggctag ttgcattaga
     3781 gagtcagcta gagccctagg gctagctgtt tgtgtattcg gagccagtgt agtttttctt
     3841 tttactaacg atacgggtaa taattgattg ccttttacag cctgaccttg agacaattgc
     3901 aaaagtctgt ccacagaggt ttgacctaat aatctaaaat cttgtttgat agtagtaagt
     3961 ggaggtatgt agcaactaga atcttctgta tcatcataac ccacaactga aatatcagcc
     4021 ccaactctaa gtccactttc agtaatagct cgcatagctc ctaaagccat ttgatcatta
     4081 gcaactaaca tagcagtagg gacaatgcct tcatttaaca tttgcattgt ttgctgaaat
     4141 cctgacatag cagaccaatc tccttctctt tctgctattg gttgtatttg atttcttgtt
     4201 aaatacttgt gccatcctgc taatctcaat ctggcactta ctgaacttaa gggacctgca
     4261 agtagtgcaa tttgttgatg gcctaatgca acaaggtgtt caactcctaa ccttgttcca
     4321 tcttcgtgag aaaaaattat agagtttatt ggggtttggt ccgacacatc caaaaacaat
     4381 gctggaacat tagtacatgc tgcttcaact gcaatcgcat cttggtcatc aagaggataa
     4441 ttaattataa gtccactaac acgttgagct aataaattat gtactgctgc cttacaagcc
     4501 tctacacctg atctctcaac cattgataca acaacgcttg caccaagttg atcagctctc
     4561 gatttaattg cggctacaat ttgactaggc gcatgtaatg caagtgatga tgtggcaact
     4621 ccaattaaaa gtgattgttt ccccgcaagt tgctgagcaa cgcgatttgg tatataattc
     4681 aactctgcca tagctgcttc aaccttctct cttgtttttg cagatacatg acttgcttgg
     4741 ttcacaactc tcgaaaccgt ttggtagcta accccggcat attcagcaac atcatacaaa
     4801 gtaacaggtt tcatggatca ttcctccatg ataaaattta aatttctgac tttggcaaat
     4861 ccttttgcct gttaaaaaga atagcacaaa aattaaaaaa aaagaaacgc aaatagcaat
     4921 agcattaaaa ataattatta ttatttattt ctttgattaa aatcaaaagc ctttaatgta
     4981 ttttcttgat ctttcatgcg cttaacggta aaatccaagg tattaagttc accttccact
     5041 tttctctctc tatcttctct agaaaatcat aaaaaattta tttgctttgt gagcggataa
     5101 caattataat agattcaatt gtgagcggat aacaatttca cacagaattc attaaagagg
     5161 agaaattaca tatggataag aaatactcaa taggcttagc tatcggcaca aatagcgtcg
     5221 gatgggcggt gatcactgat gaatataagg ttccgtctaa aaagttcaag gttctgggaa
     5281 atacagaccg ccacagtatc aaaaaaaatc ttataggggc tcttttattt gacagtggag
     5341 agacagcgga agcgactcgt ctcaaacgga cagctcgtag aaggtataca cgtcggaaga
     5401 atcgtatttg ttatctacag gagatttttt caaatgagat ggcgaaagta gatgatagtt
     5461 tctttcatcg acttgaagag tcttttttgg tggaagaaga caagaagcat gaacgtcatc
     5521 ctatttttgg aaatatagta gatgaagttg cttatcatga gaaatatcca actatctatc
     5581 atctgcgaaa aaaattggta gattctactg ataaagcgga tttgcgctta atctatttgg
     5641 ccttagcgca tatgattaag tttcgtggtc attttttgat tgagggagat ttaaatcctg
     5701 ataatagtga tgtggacaaa ctatttatcc agttggtaca aacctacaat caattatttg
     5761 aagaaaaccc tattaacgca agtggagtag atgctaaagc gattctttct gcacgattga
     5821 gtaaatcaag acgattagaa aatctcattg ctcagctccc cggtgagaag aaaaatggct
     5881 tatttgggaa tctcattgct ttgtcattgg gtttgacccc taattttaaa tcaaattttg
     5941 atttggcaga agatgctaaa ttacagcttt caaaagatac ttacgatgat gatttagata
     6001 atttattggc gcaaattgga gatcaatatg ctgatttgtt tttggcagct aagaatttat
     6061 cagatgctat tttactttca gatatcctaa gagtaaatac tgaaataact aaggctcccc
     6121 tatcagcttc aatgattaaa cgctacgatg aacatcatca agacttgact cttttaaaag
     6181 ctttagttcg acaacaactt ccagaaaagt ataaagaaat cttttttgat caatcaaaaa
     6241 acggatatgc aggttatatt gatgggggag ctagccaaga agaattttat aaatttatca
     6301 aaccaatttt agaaaaaatg gatggtactg aggaattatt ggtgaaacta aatcgtgaag
     6361 atttgctgcg caagcaacgg acctttgaca acggctctat tccccatcaa attcacttgg
     6421 gtgagctgca tgctattttg agaagacaag aagactttta tccattttta aaagacaatc
     6481 gtgagaagat tgaaaaaatc ttgacttttc gaattcctta ttatgttggt ccattggcgc
     6541 gtggcaatag tcgttttgca tggatgactc ggaagtctga agaaacaatt accccatgga
     6601 attttgaaga agttgtcgat aaaggtgctt cagctcaatc atttattgaa cgcatgacaa
     6661 actttgataa aaatcttcca aatgaaaaag tactaccaaa acatagtttg ctttatgagt
     6721 attttacggt ttataacgaa ttgacaaagg tcaaatatgt tactgaagga atgcgaaaac
     6781 cagcatttct ttcaggtgaa cagaagaaag ccattgttga tttactcttc aaaacaaatc
     6841 gaaaagtaac cgttaagcaa ttaaaagaag attatttcaa aaaaatagaa tgttttgata
     6901 gtgttgaaat ttcaggagtt gaagatagat ttaatgcttc attaggtacc taccatgatt
     6961 tgctaaaaat tattaaagat aaagattttt tggataatga agaaaatgaa gatatcttag
     7021 aggatattgt tttaacattg accttatttg aagataggga gatgattgag gaaagactta
     7081 aaacatatgc tcacctcttt gatgataagg tgatgaaaca gcttaaacgt cgccgttata
     7141 ctggttgggg acgtttgtct cgaaaattga ttaatggtat tagggataag caatctggca
     7201 aaacaatatt agattttttg aaatcagatg gttttgccaa tcgcaatttt atgcagctga
     7261 tccatgatga tagtttgaca tttaaagaag acattcaaaa agcacaagtg tctggacaag
     7321 gcgatagttt acatgaacat attgcaaatt tagctggtag ccctgctatt aaaaaaggta
     7381 ttttacagac tgtaaaagtt gttgatgaat tggtcaaagt aatggggcgg cataagccag
     7441 aaaatatcgt tattgaaatg gcacgtgaaa atcagacaac tcaaaagggc cagaaaaatt
     7501 cgcgagagcg tatgaaacga atcgaagaag gtatcaaaga attaggaagt cagattctta
     7561 aagagcatcc tgttgaaaat actcaattgc aaaatgaaaa gctctatctc tattatctcc
     7621 aaaatggaag agacatgtat gtggaccaag aattagatat taatcgttta agtgattatg
     7681 atgtcgatgc cattgttcca caaagtttcc ttaaagacga ttcaatagac aataaggtct
     7741 taacgcgttc tgataaaaat cgtggtaaat cggataacgt tccaagtgaa gaagtagtca
     7801 aaaagatgaa aaactattgg agacaacttc taaacgccaa gttaatcact caacgtaagt
     7861 ttgataattt aacgaaagct gaacgtggag gtttgagtga acttgataaa gctggtttta
     7921 tcaaacgcca attggttgaa actcgccaaa tcactaagca tgtggcacaa attttggata
     7981 gtcgcatgaa tactaaatac gatgaaaatg ataaacttat tcgagaggtt aaagtgatta
     8041 ccttaaaatc taaattagtt tctgacttcc gaaaagattt ccaattctat aaagtacgtg
     8101 agattaacaa ttaccatcat gcccatgatg cgtatctaaa tgccgtcgtt ggaactgctt
     8161 tgattaagaa atatccaaaa cttgaatcgg agtttgtcta tggtgattat aaagtttatg
     8221 atgttcgtaa aatgattgct aagtctgagc aagaaatagg caaagcaacc gcaaaatatt
     8281 tcttttactc taatatcatg aacttcttca aaacagaaat tacacttgca aatggagaga
     8341 ttcgcaaacg ccctctaatc gaaactaatg gggaaactgg agaaattgtc tgggataaag
     8401 ggcgagattt tgccacagtg cgcaaagtat tgtccatgcc ccaagtcaat attgtcaaga
     8461 aaacagaagt acagacaggc ggattctcca aggagtcaat tttaccaaaa agaaattcgg
     8521 acaagcttat tgctcgtaaa aaagactggg atccaaaaaa atatggtggt tttgatagtc
     8581 caacggtagc ttattcagtc ctagtggttg ctaaggtgga aaaagggaaa tcgaagaagt
     8641 taaaatccgt taaagagtta ctagggatca caattatgga aagaagttcc tttgaaaaaa
     8701 atccgattga ctttttagaa gctaaaggat ataaggaagt taaaaaagac ttaatcatta
     8761 aactacctaa atatagtctt tttgagttag aaaacggtcg taaacggatg ctggctagtg
     8821 ccggagaatt acaaaaagga aatgagctgg ctctgccaag caaatatgtg aattttttat
     8881 atttagctag tcattatgaa aagttgaagg gtagtccaga agataacgaa caaaaacaat
     8941 tgtttgtgga gcagcataag cattatttag atgagattat tgagcaaatc agtgaatttt
     9001 ctaagcgtgt tattttagca gatgccaatt tagataaagt tcttagtgca tataacaaac
     9061 atagagacaa accaatacgt gaacaagcag aaaatattat tcatttattt acgttgacga
     9121 atcttggagc tcccgctgct tttaaatatt ttgatacaac aattgatcgt aaacgatata
     9181 cgtctacaaa agaagtttta gatgccactc ttatccatca atccatcact ggtctttatg
     9241 aaacacgcat tgatttgagt cagctaggag gtgacacgcg tttggagcaa gtgaagttct
     9301 ggagggataa aaattaataa aagcttgatc agatctcagc ttttttttga agtgcctggc
     9361 agtaagttga aaaaatttga aataaacttg gactatgtta ataaaaaaat acaaattaac
     9421 gaacaaaaag atttgaatta ttatataatt tagttaaaag caattttaaa tgagggaggt
     9481 ttccatatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
     9541 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
     9601 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt
     9661 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
     9721 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
     9781 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
     9841 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
     9901 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
     9961 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
    10021 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
    10081 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
    10141 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc
    10201 ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc
    10261 aaggtagtcg gcaaataatg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
    10321 gttctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
    10381 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
    10441 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
    10501 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
    10561 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
    10621 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
    10681 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
    10741 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
    10801 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    10861 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    10921 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    10981 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tgggcttttg ctggcctttt
    11041 gctcacatgt tcttt
//
