LOCUS       sequence-292393-       10955 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  all-in-one CRISPRi vector for targeting B. burgdorferi flaB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10955)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 10955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S_P0026-sgRNAflaB
FEATURES             Location/Qualifiers
     source          1..10955
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        3488..3563
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(5006..5020,5021..5026,5027..5043,5044..5050)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    5026..5042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5058..5074
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5093..5104
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(5111..5137,5138..5140,5141..7627,7628..7630,
                     7631..9217)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9387..10178
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      10301..10889
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10790..10809
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcactag atccccggca agttcatcct tagggtttcc atatctctta aaaagtcaat
       61 ttctgcttga ttaatttctt gtggattatt gtttttgcgg tttttcattt tttatctcct
      121 tgagtattga attttttctt atatataaat atatagcaaa aactattttt gccaactttt
      181 ttacaaaaaa ttttttatta aaacacttag ggctttgcta aattctcttt taaaagaact
      241 tagtaaagcc ctatggattt aagaactgat tatacttacg tagtaaaaat actacagata
      301 ttaatagtat agaccatatc aaaatcataa tcaatttata tttatctaaa attgtagtcc
      361 atacaaaata caaaaaaatt aggtttttct aaattctatt ttaaaagaac ttagtaacac
      421 ctaatctcta aattacttac aaaattctaa aattagattt ttagttcttc atatttcttt
      481 aaaagttttt taagaaaatc tttttgattt tcaaaaattt catgcatcat aaaactagta
      541 aatttggatt tttttttgta aaaatcataa ctttcttggg ttttaagttg aaatcttaat
      601 ggttttattg gattttgcct agattttttt aacgccggac tttctttatc ttttataaaa
      661 ttataagaat ttttaatacc attttctatt aaataatctt cttttataag cccctcttgc
      721 agtgcagttg ctaatcttaa atacttatac gcttgagttt ttgctacatc aaagtctttt
      781 ataaaatcaa gaaaagtttt ataaccatca aatttataat attgattatc ttttatttct
      841 tttaaaattt taatattttc caccctgtgg aatatttctt ttttgaaatt gtcatttaac
      901 ttttccttta gtgtcttgta ataagaaatt ttactttcat taatcattaa attttgttca
      961 acataagtat ctatcttttt tgttaaaact ttaacaacac gttttttttc cataaaaatt
     1021 ttcctttatt tttgcaacat tttccttaat tgatataatt gttactattt tagatttttt
     1081 aatatccact taagaattaa tccctcacta gaatattttt tattatagaa ttcatataca
     1141 tgtttttcta aatctataaa tttatcaaca attgctttat tatagggtgt atctatcttt
     1201 tcttttctta ataatcgtct catattcttt aaataacaac aaacactgcc ggttttaaac
     1261 ctaaattcta tgtaatatat ttttgaaaaa gtatatacat tttcaacccc gtttatttca
     1321 tactttacaa aaacattatt tattggtttt ctataaccat agtaaatgcc taaaaatttg
     1381 tccccgtctt tcataggata tagataaagt ttttgaatct ctgatttatc taataatttt
     1441 tcaaaaaaaa ttatacaccg cttttgtttg ttactaacta ctctatgcaa tgcctttaat
     1501 atttttgtgt gatatatatt tttatcacct tttttttctt tcagcaggaa gatactttct
     1561 tttggaaatt taattttttg ttcatttatt cttgcagcct taagtccgat tttcatattt
     1621 aatccttata tagcctttct atttaattct tttaaactca ataaactttt ttggtttttc
     1681 attagttcta ataattcgca ataatatgtg caaaacactt tattgtattc tattttcttt
     1741 tggttattca aatattcttt tataattggc tttagaagtt caatatttac ttcttttttt
     1801 agttgttcaa taaggatatt gaaaatgttt gtttttaaat cttgataatt ttgttgagaa
     1861 ttttcttttt ttctttcaat tgacttctct aatttacgct ttatattgtt taaatcgcta
     1921 tatttatgac tttcaataat aaaatgaggc ttaaatttgt aattttcgta tattttttgg
     1981 aaacttgttt ctaattgttt agaattatat tcattttttt caaattcttt ttgagtgttg
     2041 catagaattt cctttaattt ttcttgtttt tctttcatac aagatttagt aaaatgtata
     2101 tttttatttt ttagtaattt aatttctatt atttttatga ttttaagtat tttgattata
     2161 ttatctttat caatatttaa attgaataaa attgtaagaa aaattgaaag aattttttta
     2221 cataaaaagt tacatttatt gaagtaattt cttacttgat acttttctat ttcattaatc
     2281 tttctttctt cttttatatt atttttatta tttaaacact cctttaaatc tacactccca
     2341 tttttagtaa atctatcttt aaggcccctt gaaactctat tttgaaatct taaatctttt
     2401 ttttcttgaa agtatttata gattttttgg taacattctt tttttgaaaa attaagttga
     2461 tagtatattt cggtccccaa atttacccct aagtgtttgt gataattagt tgtgacttta
     2521 aattcttttt ctaatttgta aaggtaattt tgcagtgttt ttagtttaac gggtgtttga
     2581 ctatttcttt taaggttttc attaaagtaa tagagtatgt ttttttgtgt gtatttctta
     2641 tatgttttat ttatgtacgc tagtgttgag ataagaacta ctaatttata ctgtaattta
     2701 ttgtagcaag ttgtggtttt actggttttg tttatattag ataagttttc catttatagc
     2761 tccttgaata ccggattttt tatctataaa gtatatatat cacaattttg atttaaaagt
     2821 aaattctttt tttaaaaata caaaaatttt ttataaatta tatagaccat gatattgtat
     2881 taatactaat tgattttaat ttaaggttga actaaaataa acatagtttt gtagaaaata
     2941 atttttcatt attttgctac ttgatattgg atcgtaaaaa tattagggct ttacaaagtt
     3001 cttttaaaag agaatttagt aaagccctaa gtcttttaat aaaaaatttt ttgtaaaaaa
     3061 gttggcaaaa atagtttttg ctatatattt atatataaga aaaaattcaa tactcaagga
     3121 gataaaaaat ggaaaactta aacaataatc cacaaaaaat taatcaaata aataagcaaa
     3181 ctaattttat tggttttgaa aattttatag aacttgataa tcaagttaat caaactaaag
     3241 aaaaaagtaa attaagtaag ataggtaaga aattacccgg aataagcagt caagtctagt
     3301 ctagtgcggc cggaaacagc tatgacatga ttacgaattc gagctctatt ctccattctt
     3361 ttaaaattat tatccttgtt tattatagtt taaaataaat ttaattacaa tttgaaattt
     3421 ggtcgtttga ctttccttat attgacaatc taagtataat attaaggaaa atttaaattt
     3481 ctgacttgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
     3541 aaaaagtggc accgagtcgg tgcttttttt ggcgcgccaa ataatgcccg tgtccctgtt
     3601 gaatagggac acggtttttt aacaattttt ttattcctag gccttagtgg ccggtacccg
     3661 gggatcctta ttactggccg ctttctagcc tggatacttg tctggctagt tgcattagag
     3721 agtcagctag agccctaggg ctagctgttt gtgtattcgg agccagtgta gtttttcttt
     3781 ttactaacga tacgggtaat aattgattgc cttttacagc ctgaccttga gacaattgca
     3841 aaagtctgtc cacagaggtt tgacctaata atctaaaatc ttgtttgata gtagtaagtg
     3901 gaggtatgta gcaactagaa tcttctgtat catcataacc cacaactgaa atatcagccc
     3961 caactctaag tccactttca gtaatagctc gcatagctcc taaagccatt tgatcattag
     4021 caactaacat agcagtaggg acaatgcctt catttaacat ttgcattgtt tgctgaaatc
     4081 ctgacatagc agaccaatct ccttctcttt ctgctattgg ttgtatttga tttcttgtta
     4141 aatacttgtg ccatcctgct aatctcaatc tggcacttac tgaacttaag ggacctgcaa
     4201 gtagtgcaat ttgttgatgg cctaatgcaa caaggtgttc aactcctaac cttgttccat
     4261 cttcgtgaga aaaaattata gagtttattg gggtttggtc cgacacatcc aaaaacaatg
     4321 ctggaacatt agtacatgct gcttcaactg caatcgcatc ttggtcatca agaggataat
     4381 taattataag tccactaaca cgttgagcta ataaattatg tactgctgcc ttacaagcct
     4441 ctacacctga tctctcaacc attgatacaa caacgcttgc accaagttga tcagctctcg
     4501 atttaattgc ggctacaatt tgactaggcg catgtaatgc aagtgatgat gtggcaactc
     4561 caattaaaag tgattgtttc cccgcaagtt gctgagcaac gcgatttggt atataattca
     4621 actctgccat agctgcttca accttctctc ttgtttttgc agatacatga cttgcttggt
     4681 tcacaactct cgaaaccgtt tggtagctaa ccccggcata ttcagcaaca tcatacaaag
     4741 taacaggttt catggatcat tcctccatga taaaatttaa atttctgact ttggcaaatc
     4801 cttttgcctg ttaaaaagaa tagcacaaaa attaaaaaaa aagaaacgca aatagcaata
     4861 gcattaaaaa taattattat tatttatttc tttgattaaa atcaaaagcc tttaatgtat
     4921 tttcttgatc tttcatgcgc ttaacggtaa aatccaaggt attaagttca ccttccactt
     4981 ttctctctct atcttctcta gaaaatcata aaaaatttat ttgctttgtg agcggataac
     5041 aattataata gattcaattg tgagcggata acaatttcac acagaattca ttaaagagga
     5101 gaaattacat atggataaga aatactcaat aggcttagct atcggcacaa atagcgtcgg
     5161 atgggcggtg atcactgatg aatataaggt tccgtctaaa aagttcaagg ttctgggaaa
     5221 tacagaccgc cacagtatca aaaaaaatct tataggggct cttttatttg acagtggaga
     5281 gacagcggaa gcgactcgtc tcaaacggac agctcgtaga aggtatacac gtcggaagaa
     5341 tcgtatttgt tatctacagg agattttttc aaatgagatg gcgaaagtag atgatagttt
     5401 ctttcatcga cttgaagagt cttttttggt ggaagaagac aagaagcatg aacgtcatcc
     5461 tatttttgga aatatagtag atgaagttgc ttatcatgag aaatatccaa ctatctatca
     5521 tctgcgaaaa aaattggtag attctactga taaagcggat ttgcgcttaa tctatttggc
     5581 cttagcgcat atgattaagt ttcgtggtca ttttttgatt gagggagatt taaatcctga
     5641 taatagtgat gtggacaaac tatttatcca gttggtacaa acctacaatc aattatttga
     5701 agaaaaccct attaacgcaa gtggagtaga tgctaaagcg attctttctg cacgattgag
     5761 taaatcaaga cgattagaaa atctcattgc tcagctcccc ggtgagaaga aaaatggctt
     5821 atttgggaat ctcattgctt tgtcattggg tttgacccct aattttaaat caaattttga
     5881 tttggcagaa gatgctaaat tacagctttc aaaagatact tacgatgatg atttagataa
     5941 tttattggcg caaattggag atcaatatgc tgatttgttt ttggcagcta agaatttatc
     6001 agatgctatt ttactttcag atatcctaag agtaaatact gaaataacta aggctcccct
     6061 atcagcttca atgattaaac gctacgatga acatcatcaa gacttgactc ttttaaaagc
     6121 tttagttcga caacaacttc cagaaaagta taaagaaatc ttttttgatc aatcaaaaaa
     6181 cggatatgca ggttatattg atgggggagc tagccaagaa gaattttata aatttatcaa
     6241 accaatttta gaaaaaatgg atggtactga ggaattattg gtgaaactaa atcgtgaaga
     6301 tttgctgcgc aagcaacgga cctttgacaa cggctctatt ccccatcaaa ttcacttggg
     6361 tgagctgcat gctattttga gaagacaaga agacttttat ccatttttaa aagacaatcg
     6421 tgagaagatt gaaaaaatct tgacttttcg aattccttat tatgttggtc cattggcgcg
     6481 tggcaatagt cgttttgcat ggatgactcg gaagtctgaa gaaacaatta ccccatggaa
     6541 ttttgaagaa gttgtcgata aaggtgcttc agctcaatca tttattgaac gcatgacaaa
     6601 ctttgataaa aatcttccaa atgaaaaagt actaccaaaa catagtttgc tttatgagta
     6661 ttttacggtt tataacgaat tgacaaaggt caaatatgtt actgaaggaa tgcgaaaacc
     6721 agcatttctt tcaggtgaac agaagaaagc cattgttgat ttactcttca aaacaaatcg
     6781 aaaagtaacc gttaagcaat taaaagaaga ttatttcaaa aaaatagaat gttttgatag
     6841 tgttgaaatt tcaggagttg aagatagatt taatgcttca ttaggtacct accatgattt
     6901 gctaaaaatt attaaagata aagatttttt ggataatgaa gaaaatgaag atatcttaga
     6961 ggatattgtt ttaacattga ccttatttga agatagggag atgattgagg aaagacttaa
     7021 aacatatgct cacctctttg atgataaggt gatgaaacag cttaaacgtc gccgttatac
     7081 tggttgggga cgtttgtctc gaaaattgat taatggtatt agggataagc aatctggcaa
     7141 aacaatatta gattttttga aatcagatgg ttttgccaat cgcaatttta tgcagctgat
     7201 ccatgatgat agtttgacat ttaaagaaga cattcaaaaa gcacaagtgt ctggacaagg
     7261 cgatagttta catgaacata ttgcaaattt agctggtagc cctgctatta aaaaaggtat
     7321 tttacagact gtaaaagttg ttgatgaatt ggtcaaagta atggggcggc ataagccaga
     7381 aaatatcgtt attgaaatgg cacgtgaaaa tcagacaact caaaagggcc agaaaaattc
     7441 gcgagagcgt atgaaacgaa tcgaagaagg tatcaaagaa ttaggaagtc agattcttaa
     7501 agagcatcct gttgaaaata ctcaattgca aaatgaaaag ctctatctct attatctcca
     7561 aaatggaaga gacatgtatg tggaccaaga attagatatt aatcgtttaa gtgattatga
     7621 tgtcgatgcc attgttccac aaagtttcct taaagacgat tcaatagaca ataaggtctt
     7681 aacgcgttct gataaaaatc gtggtaaatc ggataacgtt ccaagtgaag aagtagtcaa
     7741 aaagatgaaa aactattgga gacaacttct aaacgccaag ttaatcactc aacgtaagtt
     7801 tgataattta acgaaagctg aacgtggagg tttgagtgaa cttgataaag ctggttttat
     7861 caaacgccaa ttggttgaaa ctcgccaaat cactaagcat gtggcacaaa ttttggatag
     7921 tcgcatgaat actaaatacg atgaaaatga taaacttatt cgagaggtta aagtgattac
     7981 cttaaaatct aaattagttt ctgacttccg aaaagatttc caattctata aagtacgtga
     8041 gattaacaat taccatcatg cccatgatgc gtatctaaat gccgtcgttg gaactgcttt
     8101 gattaagaaa tatccaaaac ttgaatcgga gtttgtctat ggtgattata aagtttatga
     8161 tgttcgtaaa atgattgcta agtctgagca agaaataggc aaagcaaccg caaaatattt
     8221 cttttactct aatatcatga acttcttcaa aacagaaatt acacttgcaa atggagagat
     8281 tcgcaaacgc cctctaatcg aaactaatgg ggaaactgga gaaattgtct gggataaagg
     8341 gcgagatttt gccacagtgc gcaaagtatt gtccatgccc caagtcaata ttgtcaagaa
     8401 aacagaagta cagacaggcg gattctccaa ggagtcaatt ttaccaaaaa gaaattcgga
     8461 caagcttatt gctcgtaaaa aagactggga tccaaaaaaa tatggtggtt ttgatagtcc
     8521 aacggtagct tattcagtcc tagtggttgc taaggtggaa aaagggaaat cgaagaagtt
     8581 aaaatccgtt aaagagttac tagggatcac aattatggaa agaagttcct ttgaaaaaaa
     8641 tccgattgac tttttagaag ctaaaggata taaggaagtt aaaaaagact taatcattaa
     8701 actacctaaa tatagtcttt ttgagttaga aaacggtcgt aaacggatgc tggctagtgc
     8761 cggagaatta caaaaaggaa atgagctggc tctgccaagc aaatatgtga attttttata
     8821 tttagctagt cattatgaaa agttgaaggg tagtccagaa gataacgaac aaaaacaatt
     8881 gtttgtggag cagcataagc attatttaga tgagattatt gagcaaatca gtgaattttc
     8941 taagcgtgtt attttagcag atgccaattt agataaagtt cttagtgcat ataacaaaca
     9001 tagagacaaa ccaatacgtg aacaagcaga aaatattatt catttattta cgttgacgaa
     9061 tcttggagct cccgctgctt ttaaatattt tgatacaaca attgatcgta aacgatatac
     9121 gtctacaaaa gaagttttag atgccactct tatccatcaa tccatcactg gtctttatga
     9181 aacacgcatt gatttgagtc agctaggagg tgactaataa aagcttgatc agatctcagc
     9241 ttttttttga agtgcctggc agtaagttga aaaaatttga aataaacttg gactatgtta
     9301 ataaaaaaat acaaattaac gaacaaaaag atttgaatta ttatataatt tagttaaaag
     9361 caattttaaa tgagggaggt ttccatatga gggaagcggt gatcgccgaa gtatcgactc
     9421 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     9481 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
     9541 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     9601 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     9661 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     9721 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     9781 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     9841 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     9901 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     9961 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
    10021 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
    10081 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact
    10141 acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg acgtcgccgg agcggtcgag
    10201 ttctggaccg accggctcgg gttctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    10261 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    10321 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    10381 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    10441 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    10501 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    10561 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    10621 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    10681 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    10741 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    10801 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    10861 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    10921 tgggcttttg ctggcctttt gctcacatgt tcttt
//
