LOCUS       sequence-292394-       11017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  all-in-one CRISPRi vector for targeting B. burgdorferi flaB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11017)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 11017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S_PresTL-sgRNAflaB
FEATURES             Location/Qualifiers
     source          1..11017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        3550..3625
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(5068..5082,5083..5088,5089..5105,5106..5112)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    5088..5104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5120..5136
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5155..5166
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(5173..5199,5200..5202,5203..7689,7690..7692,
                     7693..9279)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9449..10240
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      10363..10951
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10852..10871
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcactag atccccggca agttcatcct tagggtttcc atatctctta aaaagtcaat
       61 ttctgcttga ttaatttctt gtggattatt gtttttgcgg tttttcattt tttatctcct
      121 tgagtattga attttttctt atatataaat atatagcaaa aactattttt gccaactttt
      181 ttacaaaaaa ttttttatta aaacacttag ggctttgcta aattctcttt taaaagaact
      241 tagtaaagcc ctatggattt aagaactgat tatacttacg tagtaaaaat actacagata
      301 ttaatagtat agaccatatc aaaatcataa tcaatttata tttatctaaa attgtagtcc
      361 atacaaaata caaaaaaatt aggtttttct aaattctatt ttaaaagaac ttagtaacac
      421 ctaatctcta aattacttac aaaattctaa aattagattt ttagttcttc atatttcttt
      481 aaaagttttt taagaaaatc tttttgattt tcaaaaattt catgcatcat aaaactagta
      541 aatttggatt tttttttgta aaaatcataa ctttcttggg ttttaagttg aaatcttaat
      601 ggttttattg gattttgcct agattttttt aacgccggac tttctttatc ttttataaaa
      661 ttataagaat ttttaatacc attttctatt aaataatctt cttttataag cccctcttgc
      721 agtgcagttg ctaatcttaa atacttatac gcttgagttt ttgctacatc aaagtctttt
      781 ataaaatcaa gaaaagtttt ataaccatca aatttataat attgattatc ttttatttct
      841 tttaaaattt taatattttc caccctgtgg aatatttctt ttttgaaatt gtcatttaac
      901 ttttccttta gtgtcttgta ataagaaatt ttactttcat taatcattaa attttgttca
      961 acataagtat ctatcttttt tgttaaaact ttaacaacac gttttttttc cataaaaatt
     1021 ttcctttatt tttgcaacat tttccttaat tgatataatt gttactattt tagatttttt
     1081 aatatccact taagaattaa tccctcacta gaatattttt tattatagaa ttcatataca
     1141 tgtttttcta aatctataaa tttatcaaca attgctttat tatagggtgt atctatcttt
     1201 tcttttctta ataatcgtct catattcttt aaataacaac aaacactgcc ggttttaaac
     1261 ctaaattcta tgtaatatat ttttgaaaaa gtatatacat tttcaacccc gtttatttca
     1321 tactttacaa aaacattatt tattggtttt ctataaccat agtaaatgcc taaaaatttg
     1381 tccccgtctt tcataggata tagataaagt ttttgaatct ctgatttatc taataatttt
     1441 tcaaaaaaaa ttatacaccg cttttgtttg ttactaacta ctctatgcaa tgcctttaat
     1501 atttttgtgt gatatatatt tttatcacct tttttttctt tcagcaggaa gatactttct
     1561 tttggaaatt taattttttg ttcatttatt cttgcagcct taagtccgat tttcatattt
     1621 aatccttata tagcctttct atttaattct tttaaactca ataaactttt ttggtttttc
     1681 attagttcta ataattcgca ataatatgtg caaaacactt tattgtattc tattttcttt
     1741 tggttattca aatattcttt tataattggc tttagaagtt caatatttac ttcttttttt
     1801 agttgttcaa taaggatatt gaaaatgttt gtttttaaat cttgataatt ttgttgagaa
     1861 ttttcttttt ttctttcaat tgacttctct aatttacgct ttatattgtt taaatcgcta
     1921 tatttatgac tttcaataat aaaatgaggc ttaaatttgt aattttcgta tattttttgg
     1981 aaacttgttt ctaattgttt agaattatat tcattttttt caaattcttt ttgagtgttg
     2041 catagaattt cctttaattt ttcttgtttt tctttcatac aagatttagt aaaatgtata
     2101 tttttatttt ttagtaattt aatttctatt atttttatga ttttaagtat tttgattata
     2161 ttatctttat caatatttaa attgaataaa attgtaagaa aaattgaaag aattttttta
     2221 cataaaaagt tacatttatt gaagtaattt cttacttgat acttttctat ttcattaatc
     2281 tttctttctt cttttatatt atttttatta tttaaacact cctttaaatc tacactccca
     2341 tttttagtaa atctatcttt aaggcccctt gaaactctat tttgaaatct taaatctttt
     2401 ttttcttgaa agtatttata gattttttgg taacattctt tttttgaaaa attaagttga
     2461 tagtatattt cggtccccaa atttacccct aagtgtttgt gataattagt tgtgacttta
     2521 aattcttttt ctaatttgta aaggtaattt tgcagtgttt ttagtttaac gggtgtttga
     2581 ctatttcttt taaggttttc attaaagtaa tagagtatgt ttttttgtgt gtatttctta
     2641 tatgttttat ttatgtacgc tagtgttgag ataagaacta ctaatttata ctgtaattta
     2701 ttgtagcaag ttgtggtttt actggttttg tttatattag ataagttttc catttatagc
     2761 tccttgaata ccggattttt tatctataaa gtatatatat cacaattttg atttaaaagt
     2821 aaattctttt tttaaaaata caaaaatttt ttataaatta tatagaccat gatattgtat
     2881 taatactaat tgattttaat ttaaggttga actaaaataa acatagtttt gtagaaaata
     2941 atttttcatt attttgctac ttgatattgg atcgtaaaaa tattagggct ttacaaagtt
     3001 cttttaaaag agaatttagt aaagccctaa gtcttttaat aaaaaatttt ttgtaaaaaa
     3061 gttggcaaaa atagtttttg ctatatattt atatataaga aaaaattcaa tactcaagga
     3121 gataaaaaat ggaaaactta aacaataatc cacaaaaaat taatcaaata aataagcaaa
     3181 ctaattttat tggttttgaa aattttatag aacttgataa tcaagttaat caaactaaag
     3241 aaaaaagtaa attaagtaag ataggtaaga aattacccgg aataagcagt caagtctagt
     3301 ctagtgcggc cggaaacagc tatgacatga ttacgaattc gagctccgaa gtttattatt
     3361 ttatgattta aattaataaa aagatttatc ggtgtgaata gaatatttct ttggtttatt
     3421 atgcatccta gtacatatta tataatttaa tttaatatat aagcatattt ttttaaaatc
     3481 agaaattttt tacttagcta aagattttaa acttggtata attgaattga aaatttaaat
     3541 ttctgacttg ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact
     3601 tgaaaaagtg gcaccgagtc ggtgcttttt ttggcgcgcc aaataatgcc cgtgtccctg
     3661 ttgaataggg acacggtttt ttaacaattt ttttattcct aggccttagt ggccggtacc
     3721 cggggatcct tattactggc cgctttctag cctggatact tgtctggcta gttgcattag
     3781 agagtcagct agagccctag ggctagctgt ttgtgtattc ggagccagtg tagtttttct
     3841 ttttactaac gatacgggta ataattgatt gccttttaca gcctgacctt gagacaattg
     3901 caaaagtctg tccacagagg tttgacctaa taatctaaaa tcttgtttga tagtagtaag
     3961 tggaggtatg tagcaactag aatcttctgt atcatcataa cccacaactg aaatatcagc
     4021 cccaactcta agtccacttt cagtaatagc tcgcatagct cctaaagcca tttgatcatt
     4081 agcaactaac atagcagtag ggacaatgcc ttcatttaac atttgcattg tttgctgaaa
     4141 tcctgacata gcagaccaat ctccttctct ttctgctatt ggttgtattt gatttcttgt
     4201 taaatacttg tgccatcctg ctaatctcaa tctggcactt actgaactta agggacctgc
     4261 aagtagtgca atttgttgat ggcctaatgc aacaaggtgt tcaactccta accttgttcc
     4321 atcttcgtga gaaaaaatta tagagtttat tggggtttgg tccgacacat ccaaaaacaa
     4381 tgctggaaca ttagtacatg ctgcttcaac tgcaatcgca tcttggtcat caagaggata
     4441 attaattata agtccactaa cacgttgagc taataaatta tgtactgctg ccttacaagc
     4501 ctctacacct gatctctcaa ccattgatac aacaacgctt gcaccaagtt gatcagctct
     4561 cgatttaatt gcggctacaa tttgactagg cgcatgtaat gcaagtgatg atgtggcaac
     4621 tccaattaaa agtgattgtt tccccgcaag ttgctgagca acgcgatttg gtatataatt
     4681 caactctgcc atagctgctt caaccttctc tcttgttttt gcagatacat gacttgcttg
     4741 gttcacaact ctcgaaaccg tttggtagct aaccccggca tattcagcaa catcatacaa
     4801 agtaacaggt ttcatggatc attcctccat gataaaattt aaatttctga ctttggcaaa
     4861 tccttttgcc tgttaaaaag aatagcacaa aaattaaaaa aaaagaaacg caaatagcaa
     4921 tagcattaaa aataattatt attatttatt tctttgatta aaatcaaaag cctttaatgt
     4981 attttcttga tctttcatgc gcttaacggt aaaatccaag gtattaagtt caccttccac
     5041 ttttctctct ctatcttctc tagaaaatca taaaaaattt atttgctttg tgagcggata
     5101 acaattataa tagattcaat tgtgagcgga taacaatttc acacagaatt cattaaagag
     5161 gagaaattac atatggataa gaaatactca ataggcttag ctatcggcac aaatagcgtc
     5221 ggatgggcgg tgatcactga tgaatataag gttccgtcta aaaagttcaa ggttctggga
     5281 aatacagacc gccacagtat caaaaaaaat cttatagggg ctcttttatt tgacagtgga
     5341 gagacagcgg aagcgactcg tctcaaacgg acagctcgta gaaggtatac acgtcggaag
     5401 aatcgtattt gttatctaca ggagattttt tcaaatgaga tggcgaaagt agatgatagt
     5461 ttctttcatc gacttgaaga gtcttttttg gtggaagaag acaagaagca tgaacgtcat
     5521 cctatttttg gaaatatagt agatgaagtt gcttatcatg agaaatatcc aactatctat
     5581 catctgcgaa aaaaattggt agattctact gataaagcgg atttgcgctt aatctatttg
     5641 gccttagcgc atatgattaa gtttcgtggt cattttttga ttgagggaga tttaaatcct
     5701 gataatagtg atgtggacaa actatttatc cagttggtac aaacctacaa tcaattattt
     5761 gaagaaaacc ctattaacgc aagtggagta gatgctaaag cgattctttc tgcacgattg
     5821 agtaaatcaa gacgattaga aaatctcatt gctcagctcc ccggtgagaa gaaaaatggc
     5881 ttatttggga atctcattgc tttgtcattg ggtttgaccc ctaattttaa atcaaatttt
     5941 gatttggcag aagatgctaa attacagctt tcaaaagata cttacgatga tgatttagat
     6001 aatttattgg cgcaaattgg agatcaatat gctgatttgt ttttggcagc taagaattta
     6061 tcagatgcta ttttactttc agatatccta agagtaaata ctgaaataac taaggctccc
     6121 ctatcagctt caatgattaa acgctacgat gaacatcatc aagacttgac tcttttaaaa
     6181 gctttagttc gacaacaact tccagaaaag tataaagaaa tcttttttga tcaatcaaaa
     6241 aacggatatg caggttatat tgatggggga gctagccaag aagaatttta taaatttatc
     6301 aaaccaattt tagaaaaaat ggatggtact gaggaattat tggtgaaact aaatcgtgaa
     6361 gatttgctgc gcaagcaacg gacctttgac aacggctcta ttccccatca aattcacttg
     6421 ggtgagctgc atgctatttt gagaagacaa gaagactttt atccattttt aaaagacaat
     6481 cgtgagaaga ttgaaaaaat cttgactttt cgaattcctt attatgttgg tccattggcg
     6541 cgtggcaata gtcgttttgc atggatgact cggaagtctg aagaaacaat taccccatgg
     6601 aattttgaag aagttgtcga taaaggtgct tcagctcaat catttattga acgcatgaca
     6661 aactttgata aaaatcttcc aaatgaaaaa gtactaccaa aacatagttt gctttatgag
     6721 tattttacgg tttataacga attgacaaag gtcaaatatg ttactgaagg aatgcgaaaa
     6781 ccagcatttc tttcaggtga acagaagaaa gccattgttg atttactctt caaaacaaat
     6841 cgaaaagtaa ccgttaagca attaaaagaa gattatttca aaaaaataga atgttttgat
     6901 agtgttgaaa tttcaggagt tgaagataga tttaatgctt cattaggtac ctaccatgat
     6961 ttgctaaaaa ttattaaaga taaagatttt ttggataatg aagaaaatga agatatctta
     7021 gaggatattg ttttaacatt gaccttattt gaagataggg agatgattga ggaaagactt
     7081 aaaacatatg ctcacctctt tgatgataag gtgatgaaac agcttaaacg tcgccgttat
     7141 actggttggg gacgtttgtc tcgaaaattg attaatggta ttagggataa gcaatctggc
     7201 aaaacaatat tagatttttt gaaatcagat ggttttgcca atcgcaattt tatgcagctg
     7261 atccatgatg atagtttgac atttaaagaa gacattcaaa aagcacaagt gtctggacaa
     7321 ggcgatagtt tacatgaaca tattgcaaat ttagctggta gccctgctat taaaaaaggt
     7381 attttacaga ctgtaaaagt tgttgatgaa ttggtcaaag taatggggcg gcataagcca
     7441 gaaaatatcg ttattgaaat ggcacgtgaa aatcagacaa ctcaaaaggg ccagaaaaat
     7501 tcgcgagagc gtatgaaacg aatcgaagaa ggtatcaaag aattaggaag tcagattctt
     7561 aaagagcatc ctgttgaaaa tactcaattg caaaatgaaa agctctatct ctattatctc
     7621 caaaatggaa gagacatgta tgtggaccaa gaattagata ttaatcgttt aagtgattat
     7681 gatgtcgatg ccattgttcc acaaagtttc cttaaagacg attcaataga caataaggtc
     7741 ttaacgcgtt ctgataaaaa tcgtggtaaa tcggataacg ttccaagtga agaagtagtc
     7801 aaaaagatga aaaactattg gagacaactt ctaaacgcca agttaatcac tcaacgtaag
     7861 tttgataatt taacgaaagc tgaacgtgga ggtttgagtg aacttgataa agctggtttt
     7921 atcaaacgcc aattggttga aactcgccaa atcactaagc atgtggcaca aattttggat
     7981 agtcgcatga atactaaata cgatgaaaat gataaactta ttcgagaggt taaagtgatt
     8041 accttaaaat ctaaattagt ttctgacttc cgaaaagatt tccaattcta taaagtacgt
     8101 gagattaaca attaccatca tgcccatgat gcgtatctaa atgccgtcgt tggaactgct
     8161 ttgattaaga aatatccaaa acttgaatcg gagtttgtct atggtgatta taaagtttat
     8221 gatgttcgta aaatgattgc taagtctgag caagaaatag gcaaagcaac cgcaaaatat
     8281 ttcttttact ctaatatcat gaacttcttc aaaacagaaa ttacacttgc aaatggagag
     8341 attcgcaaac gccctctaat cgaaactaat ggggaaactg gagaaattgt ctgggataaa
     8401 gggcgagatt ttgccacagt gcgcaaagta ttgtccatgc cccaagtcaa tattgtcaag
     8461 aaaacagaag tacagacagg cggattctcc aaggagtcaa ttttaccaaa aagaaattcg
     8521 gacaagctta ttgctcgtaa aaaagactgg gatccaaaaa aatatggtgg ttttgatagt
     8581 ccaacggtag cttattcagt cctagtggtt gctaaggtgg aaaaagggaa atcgaagaag
     8641 ttaaaatccg ttaaagagtt actagggatc acaattatgg aaagaagttc ctttgaaaaa
     8701 aatccgattg actttttaga agctaaagga tataaggaag ttaaaaaaga cttaatcatt
     8761 aaactaccta aatatagtct ttttgagtta gaaaacggtc gtaaacggat gctggctagt
     8821 gccggagaat tacaaaaagg aaatgagctg gctctgccaa gcaaatatgt gaatttttta
     8881 tatttagcta gtcattatga aaagttgaag ggtagtccag aagataacga acaaaaacaa
     8941 ttgtttgtgg agcagcataa gcattattta gatgagatta ttgagcaaat cagtgaattt
     9001 tctaagcgtg ttattttagc agatgccaat ttagataaag ttcttagtgc atataacaaa
     9061 catagagaca aaccaatacg tgaacaagca gaaaatatta ttcatttatt tacgttgacg
     9121 aatcttggag ctcccgctgc ttttaaatat tttgatacaa caattgatcg taaacgatat
     9181 acgtctacaa aagaagtttt agatgccact cttatccatc aatccatcac tggtctttat
     9241 gaaacacgca ttgatttgag tcagctagga ggtgactaat aaaagcttga tcagatctca
     9301 gctttttttt gaagtgcctg gcagtaagtt gaaaaaattt gaaataaact tggactatgt
     9361 taataaaaaa atacaaatta acgaacaaaa agatttgaat tattatataa tttagttaaa
     9421 agcaatttta aatgagggag gtttccatat gagggaagcg gtgatcgccg aagtatcgac
     9481 tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt
     9541 acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct
     9601 ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt
     9661 ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt
     9721 tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga
     9781 atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct
     9841 ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga
     9901 ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac
     9961 gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc
    10021 ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg
    10081 ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta
    10141 tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca
    10201 ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa tgacgtcgcc ggagcggtcg
    10261 agttctggac cgaccggctc gggttctcat gaccaaaatc ccttaacgtg agttttcgtt
    10321 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
    10381 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
    10441 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
    10501 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
    10561 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
    10621 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
    10681 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
    10741 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
    10801 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
    10861 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
    10921 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
    10981 cctgggcttt tgctggcctt ttgctcacat gttcttt
//
