LOCUS       sequence-292395-       10942 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  all-in-one CRISPRi vector for targeting B. burgdorferi flaB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10942)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 10942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S_PresTS-sgRNAflaB
FEATURES             Location/Qualifiers
     source          1..10942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        3475..3550
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(4993..5007,5008..5013,5014..5030,5031..5037)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    5013..5029
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5045..5061
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5080..5091
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(5098..5124,5125..5127,5128..7614,7615..7617,
                     7618..9204)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9374..10165
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      10288..10876
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10777..10796
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcactag atccccggca agttcatcct tagggtttcc atatctctta aaaagtcaat
       61 ttctgcttga ttaatttctt gtggattatt gtttttgcgg tttttcattt tttatctcct
      121 tgagtattga attttttctt atatataaat atatagcaaa aactattttt gccaactttt
      181 ttacaaaaaa ttttttatta aaacacttag ggctttgcta aattctcttt taaaagaact
      241 tagtaaagcc ctatggattt aagaactgat tatacttacg tagtaaaaat actacagata
      301 ttaatagtat agaccatatc aaaatcataa tcaatttata tttatctaaa attgtagtcc
      361 atacaaaata caaaaaaatt aggtttttct aaattctatt ttaaaagaac ttagtaacac
      421 ctaatctcta aattacttac aaaattctaa aattagattt ttagttcttc atatttcttt
      481 aaaagttttt taagaaaatc tttttgattt tcaaaaattt catgcatcat aaaactagta
      541 aatttggatt tttttttgta aaaatcataa ctttcttggg ttttaagttg aaatcttaat
      601 ggttttattg gattttgcct agattttttt aacgccggac tttctttatc ttttataaaa
      661 ttataagaat ttttaatacc attttctatt aaataatctt cttttataag cccctcttgc
      721 agtgcagttg ctaatcttaa atacttatac gcttgagttt ttgctacatc aaagtctttt
      781 ataaaatcaa gaaaagtttt ataaccatca aatttataat attgattatc ttttatttct
      841 tttaaaattt taatattttc caccctgtgg aatatttctt ttttgaaatt gtcatttaac
      901 ttttccttta gtgtcttgta ataagaaatt ttactttcat taatcattaa attttgttca
      961 acataagtat ctatcttttt tgttaaaact ttaacaacac gttttttttc cataaaaatt
     1021 ttcctttatt tttgcaacat tttccttaat tgatataatt gttactattt tagatttttt
     1081 aatatccact taagaattaa tccctcacta gaatattttt tattatagaa ttcatataca
     1141 tgtttttcta aatctataaa tttatcaaca attgctttat tatagggtgt atctatcttt
     1201 tcttttctta ataatcgtct catattcttt aaataacaac aaacactgcc ggttttaaac
     1261 ctaaattcta tgtaatatat ttttgaaaaa gtatatacat tttcaacccc gtttatttca
     1321 tactttacaa aaacattatt tattggtttt ctataaccat agtaaatgcc taaaaatttg
     1381 tccccgtctt tcataggata tagataaagt ttttgaatct ctgatttatc taataatttt
     1441 tcaaaaaaaa ttatacaccg cttttgtttg ttactaacta ctctatgcaa tgcctttaat
     1501 atttttgtgt gatatatatt tttatcacct tttttttctt tcagcaggaa gatactttct
     1561 tttggaaatt taattttttg ttcatttatt cttgcagcct taagtccgat tttcatattt
     1621 aatccttata tagcctttct atttaattct tttaaactca ataaactttt ttggtttttc
     1681 attagttcta ataattcgca ataatatgtg caaaacactt tattgtattc tattttcttt
     1741 tggttattca aatattcttt tataattggc tttagaagtt caatatttac ttcttttttt
     1801 agttgttcaa taaggatatt gaaaatgttt gtttttaaat cttgataatt ttgttgagaa
     1861 ttttcttttt ttctttcaat tgacttctct aatttacgct ttatattgtt taaatcgcta
     1921 tatttatgac tttcaataat aaaatgaggc ttaaatttgt aattttcgta tattttttgg
     1981 aaacttgttt ctaattgttt agaattatat tcattttttt caaattcttt ttgagtgttg
     2041 catagaattt cctttaattt ttcttgtttt tctttcatac aagatttagt aaaatgtata
     2101 tttttatttt ttagtaattt aatttctatt atttttatga ttttaagtat tttgattata
     2161 ttatctttat caatatttaa attgaataaa attgtaagaa aaattgaaag aattttttta
     2221 cataaaaagt tacatttatt gaagtaattt cttacttgat acttttctat ttcattaatc
     2281 tttctttctt cttttatatt atttttatta tttaaacact cctttaaatc tacactccca
     2341 tttttagtaa atctatcttt aaggcccctt gaaactctat tttgaaatct taaatctttt
     2401 ttttcttgaa agtatttata gattttttgg taacattctt tttttgaaaa attaagttga
     2461 tagtatattt cggtccccaa atttacccct aagtgtttgt gataattagt tgtgacttta
     2521 aattcttttt ctaatttgta aaggtaattt tgcagtgttt ttagtttaac gggtgtttga
     2581 ctatttcttt taaggttttc attaaagtaa tagagtatgt ttttttgtgt gtatttctta
     2641 tatgttttat ttatgtacgc tagtgttgag ataagaacta ctaatttata ctgtaattta
     2701 ttgtagcaag ttgtggtttt actggttttg tttatattag ataagttttc catttatagc
     2761 tccttgaata ccggattttt tatctataaa gtatatatat cacaattttg atttaaaagt
     2821 aaattctttt tttaaaaata caaaaatttt ttataaatta tatagaccat gatattgtat
     2881 taatactaat tgattttaat ttaaggttga actaaaataa acatagtttt gtagaaaata
     2941 atttttcatt attttgctac ttgatattgg atcgtaaaaa tattagggct ttacaaagtt
     3001 cttttaaaag agaatttagt aaagccctaa gtcttttaat aaaaaatttt ttgtaaaaaa
     3061 gttggcaaaa atagtttttg ctatatattt atatataaga aaaaattcaa tactcaagga
     3121 gataaaaaat ggaaaactta aacaataatc cacaaaaaat taatcaaata aataagcaaa
     3181 ctaattttat tggttttgaa aattttatag aacttgataa tcaagttaat caaactaaag
     3241 aaaaaagtaa attaagtaag ataggtaaga aattacccgg aataagcagt caagtctagt
     3301 ctagtgcggc cggaaacagc tatgacatga ttacgaattc gagctccgaa gtttattatt
     3361 ttatgattta aattaataaa aagatttatc ggtgtgaata gaatatttct ttggtttatt
     3421 atgcatccta gtacatatta tataatttaa tttgaaaatt taaatttctg acttgtttta
     3481 gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc
     3541 gagtcggtgc tttttttggc gcgccaaata atgcccgtgt ccctgttgaa tagggacacg
     3601 gttttttaac aattttttta ttcctaggcc ttagtggccg gtacccgggg atccttatta
     3661 ctggccgctt tctagcctgg atacttgtct ggctagttgc attagagagt cagctagagc
     3721 cctagggcta gctgtttgtg tattcggagc cagtgtagtt tttcttttta ctaacgatac
     3781 gggtaataat tgattgcctt ttacagcctg accttgagac aattgcaaaa gtctgtccac
     3841 agaggtttga cctaataatc taaaatcttg tttgatagta gtaagtggag gtatgtagca
     3901 actagaatct tctgtatcat cataacccac aactgaaata tcagccccaa ctctaagtcc
     3961 actttcagta atagctcgca tagctcctaa agccatttga tcattagcaa ctaacatagc
     4021 agtagggaca atgccttcat ttaacatttg cattgtttgc tgaaatcctg acatagcaga
     4081 ccaatctcct tctctttctg ctattggttg tatttgattt cttgttaaat acttgtgcca
     4141 tcctgctaat ctcaatctgg cacttactga acttaaggga cctgcaagta gtgcaatttg
     4201 ttgatggcct aatgcaacaa ggtgttcaac tcctaacctt gttccatctt cgtgagaaaa
     4261 aattatagag tttattgggg tttggtccga cacatccaaa aacaatgctg gaacattagt
     4321 acatgctgct tcaactgcaa tcgcatcttg gtcatcaaga ggataattaa ttataagtcc
     4381 actaacacgt tgagctaata aattatgtac tgctgcctta caagcctcta cacctgatct
     4441 ctcaaccatt gatacaacaa cgcttgcacc aagttgatca gctctcgatt taattgcggc
     4501 tacaatttga ctaggcgcat gtaatgcaag tgatgatgtg gcaactccaa ttaaaagtga
     4561 ttgtttcccc gcaagttgct gagcaacgcg atttggtata taattcaact ctgccatagc
     4621 tgcttcaacc ttctctcttg tttttgcaga tacatgactt gcttggttca caactctcga
     4681 aaccgtttgg tagctaaccc cggcatattc agcaacatca tacaaagtaa caggtttcat
     4741 ggatcattcc tccatgataa aatttaaatt tctgactttg gcaaatcctt ttgcctgtta
     4801 aaaagaatag cacaaaaatt aaaaaaaaag aaacgcaaat agcaatagca ttaaaaataa
     4861 ttattattat ttatttcttt gattaaaatc aaaagccttt aatgtatttt cttgatcttt
     4921 catgcgctta acggtaaaat ccaaggtatt aagttcacct tccacttttc tctctctatc
     4981 ttctctagaa aatcataaaa aatttatttg ctttgtgagc ggataacaat tataatagat
     5041 tcaattgtga gcggataaca atttcacaca gaattcatta aagaggagaa attacatatg
     5101 gataagaaat actcaatagg cttagctatc ggcacaaata gcgtcggatg ggcggtgatc
     5161 actgatgaat ataaggttcc gtctaaaaag ttcaaggttc tgggaaatac agaccgccac
     5221 agtatcaaaa aaaatcttat aggggctctt ttatttgaca gtggagagac agcggaagcg
     5281 actcgtctca aacggacagc tcgtagaagg tatacacgtc ggaagaatcg tatttgttat
     5341 ctacaggaga ttttttcaaa tgagatggcg aaagtagatg atagtttctt tcatcgactt
     5401 gaagagtctt ttttggtgga agaagacaag aagcatgaac gtcatcctat ttttggaaat
     5461 atagtagatg aagttgctta tcatgagaaa tatccaacta tctatcatct gcgaaaaaaa
     5521 ttggtagatt ctactgataa agcggatttg cgcttaatct atttggcctt agcgcatatg
     5581 attaagtttc gtggtcattt tttgattgag ggagatttaa atcctgataa tagtgatgtg
     5641 gacaaactat ttatccagtt ggtacaaacc tacaatcaat tatttgaaga aaaccctatt
     5701 aacgcaagtg gagtagatgc taaagcgatt ctttctgcac gattgagtaa atcaagacga
     5761 ttagaaaatc tcattgctca gctccccggt gagaagaaaa atggcttatt tgggaatctc
     5821 attgctttgt cattgggttt gacccctaat tttaaatcaa attttgattt ggcagaagat
     5881 gctaaattac agctttcaaa agatacttac gatgatgatt tagataattt attggcgcaa
     5941 attggagatc aatatgctga tttgtttttg gcagctaaga atttatcaga tgctatttta
     6001 ctttcagata tcctaagagt aaatactgaa ataactaagg ctcccctatc agcttcaatg
     6061 attaaacgct acgatgaaca tcatcaagac ttgactcttt taaaagcttt agttcgacaa
     6121 caacttccag aaaagtataa agaaatcttt tttgatcaat caaaaaacgg atatgcaggt
     6181 tatattgatg ggggagctag ccaagaagaa ttttataaat ttatcaaacc aattttagaa
     6241 aaaatggatg gtactgagga attattggtg aaactaaatc gtgaagattt gctgcgcaag
     6301 caacggacct ttgacaacgg ctctattccc catcaaattc acttgggtga gctgcatgct
     6361 attttgagaa gacaagaaga cttttatcca tttttaaaag acaatcgtga gaagattgaa
     6421 aaaatcttga cttttcgaat tccttattat gttggtccat tggcgcgtgg caatagtcgt
     6481 tttgcatgga tgactcggaa gtctgaagaa acaattaccc catggaattt tgaagaagtt
     6541 gtcgataaag gtgcttcagc tcaatcattt attgaacgca tgacaaactt tgataaaaat
     6601 cttccaaatg aaaaagtact accaaaacat agtttgcttt atgagtattt tacggtttat
     6661 aacgaattga caaaggtcaa atatgttact gaaggaatgc gaaaaccagc atttctttca
     6721 ggtgaacaga agaaagccat tgttgattta ctcttcaaaa caaatcgaaa agtaaccgtt
     6781 aagcaattaa aagaagatta tttcaaaaaa atagaatgtt ttgatagtgt tgaaatttca
     6841 ggagttgaag atagatttaa tgcttcatta ggtacctacc atgatttgct aaaaattatt
     6901 aaagataaag attttttgga taatgaagaa aatgaagata tcttagagga tattgtttta
     6961 acattgacct tatttgaaga tagggagatg attgaggaaa gacttaaaac atatgctcac
     7021 ctctttgatg ataaggtgat gaaacagctt aaacgtcgcc gttatactgg ttggggacgt
     7081 ttgtctcgaa aattgattaa tggtattagg gataagcaat ctggcaaaac aatattagat
     7141 tttttgaaat cagatggttt tgccaatcgc aattttatgc agctgatcca tgatgatagt
     7201 ttgacattta aagaagacat tcaaaaagca caagtgtctg gacaaggcga tagtttacat
     7261 gaacatattg caaatttagc tggtagccct gctattaaaa aaggtatttt acagactgta
     7321 aaagttgttg atgaattggt caaagtaatg gggcggcata agccagaaaa tatcgttatt
     7381 gaaatggcac gtgaaaatca gacaactcaa aagggccaga aaaattcgcg agagcgtatg
     7441 aaacgaatcg aagaaggtat caaagaatta ggaagtcaga ttcttaaaga gcatcctgtt
     7501 gaaaatactc aattgcaaaa tgaaaagctc tatctctatt atctccaaaa tggaagagac
     7561 atgtatgtgg accaagaatt agatattaat cgtttaagtg attatgatgt cgatgccatt
     7621 gttccacaaa gtttccttaa agacgattca atagacaata aggtcttaac gcgttctgat
     7681 aaaaatcgtg gtaaatcgga taacgttcca agtgaagaag tagtcaaaaa gatgaaaaac
     7741 tattggagac aacttctaaa cgccaagtta atcactcaac gtaagtttga taatttaacg
     7801 aaagctgaac gtggaggttt gagtgaactt gataaagctg gttttatcaa acgccaattg
     7861 gttgaaactc gccaaatcac taagcatgtg gcacaaattt tggatagtcg catgaatact
     7921 aaatacgatg aaaatgataa acttattcga gaggttaaag tgattacctt aaaatctaaa
     7981 ttagtttctg acttccgaaa agatttccaa ttctataaag tacgtgagat taacaattac
     8041 catcatgccc atgatgcgta tctaaatgcc gtcgttggaa ctgctttgat taagaaatat
     8101 ccaaaacttg aatcggagtt tgtctatggt gattataaag tttatgatgt tcgtaaaatg
     8161 attgctaagt ctgagcaaga aataggcaaa gcaaccgcaa aatatttctt ttactctaat
     8221 atcatgaact tcttcaaaac agaaattaca cttgcaaatg gagagattcg caaacgccct
     8281 ctaatcgaaa ctaatgggga aactggagaa attgtctggg ataaagggcg agattttgcc
     8341 acagtgcgca aagtattgtc catgccccaa gtcaatattg tcaagaaaac agaagtacag
     8401 acaggcggat tctccaagga gtcaatttta ccaaaaagaa attcggacaa gcttattgct
     8461 cgtaaaaaag actgggatcc aaaaaaatat ggtggttttg atagtccaac ggtagcttat
     8521 tcagtcctag tggttgctaa ggtggaaaaa gggaaatcga agaagttaaa atccgttaaa
     8581 gagttactag ggatcacaat tatggaaaga agttcctttg aaaaaaatcc gattgacttt
     8641 ttagaagcta aaggatataa ggaagttaaa aaagacttaa tcattaaact acctaaatat
     8701 agtctttttg agttagaaaa cggtcgtaaa cggatgctgg ctagtgccgg agaattacaa
     8761 aaaggaaatg agctggctct gccaagcaaa tatgtgaatt ttttatattt agctagtcat
     8821 tatgaaaagt tgaagggtag tccagaagat aacgaacaaa aacaattgtt tgtggagcag
     8881 cataagcatt atttagatga gattattgag caaatcagtg aattttctaa gcgtgttatt
     8941 ttagcagatg ccaatttaga taaagttctt agtgcatata acaaacatag agacaaacca
     9001 atacgtgaac aagcagaaaa tattattcat ttatttacgt tgacgaatct tggagctccc
     9061 gctgctttta aatattttga tacaacaatt gatcgtaaac gatatacgtc tacaaaagaa
     9121 gttttagatg ccactcttat ccatcaatcc atcactggtc tttatgaaac acgcattgat
     9181 ttgagtcagc taggaggtga ctaataaaag cttgatcaga tctcagcttt tttttgaagt
     9241 gcctggcagt aagttgaaaa aatttgaaat aaacttggac tatgttaata aaaaaataca
     9301 aattaacgaa caaaaagatt tgaattatta tataatttag ttaaaagcaa ttttaaatga
     9361 gggaggtttc catatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
     9421 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
     9481 cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt
     9541 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
     9601 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
     9661 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
     9721 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
     9781 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     9841 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     9901 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     9961 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
    10021 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
    10081 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga
    10141 gatcaccaag gtagtcggca aataatgacg tcgccggagc ggtcgagttc tggaccgacc
    10201 ggctcgggtt ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    10261 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
    10321 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
    10381 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
    10441 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
    10501 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
    10561 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    10621 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
    10681 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
    10741 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    10801 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    10861 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg gcttttgctg
    10921 gccttttgct cacatgttct tt
//
