LOCUS       sequence-292399-       11386 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  parental, all-in-one CRISPRi vector for B. burgdorferi, parental for
            gRNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11386)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 11386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S_Psyn-sgRNA500
FEATURES             Location/Qualifiers
     source          1..11386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3318..3352
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        3869..3944
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(5387..5401,5402..5407,5408..5424,5425..5431)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    5407..5423
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5439..5455
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5474..5485
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(5492..5518,5519..5521,5522..8008,8009..8011,
                     8012..9595)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9807..10598
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      10721..11309
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11210..11229
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tccccggcaa gttcatcctt agggtttcca tatctcttaa aaagtcaatt tctgcttgat
       61 taatttcttg tggattattg tttttgcggt ttttcatttt ttatctcctt gagtattgaa
      121 ttttttctta tatataaata tatagcaaaa actatttttg ccaacttttt tacaaaaaat
      181 tttttattaa aacacttagg gctttgctaa attctctttt aaaagaactt agtaaagccc
      241 tatggattta agaactgatt atacttacgt agtaaaaata ctacagatat taatagtata
      301 gaccatatca aaatcataat caatttatat ttatctaaaa ttgtagtcca tacaaaatac
      361 aaaaaaatta ggtttttcta aattctattt taaaagaact tagtaacacc taatctctaa
      421 attacttaca aaattctaaa attagatttt tagttcttca tatttcttta aaagtttttt
      481 aagaaaatct ttttgatttt caaaaatttc atgcatcata aaactagtaa atttggattt
      541 ttttttgtaa aaatcataac tttcttgggt tttaagttga aatcttaatg gttttattgg
      601 attttgccta gattttttta acgccggact ttctttatct tttataaaat tataagaatt
      661 tttaatacca ttttctatta aataatcttc ttttataagc ccctcttgca gtgcagttgc
      721 taatcttaaa tacttatacg cttgagtttt tgctacatca aagtctttta taaaatcaag
      781 aaaagtttta taaccatcaa atttataata ttgattatct tttatttctt ttaaaatttt
      841 aatattttcc accctgtgga atatttcttt tttgaaattg tcatttaact tttcctttag
      901 tgtcttgtaa taagaaattt tactttcatt aatcattaaa ttttgttcaa cataagtatc
      961 tatctttttt gttaaaactt taacaacacg ttttttttcc ataaaaattt tcctttattt
     1021 ttgcaacatt ttccttaatt gatataattg ttactatttt agatttttta atatccactt
     1081 aagaattaat ccctcactag aatatttttt attatagaat tcatatacat gtttttctaa
     1141 atctataaat ttatcaacaa ttgctttatt atagggtgta tctatctttt cttttcttaa
     1201 taatcgtctc atattcttta aataacaaca aacactgccg gttttaaacc taaattctat
     1261 gtaatatatt tttgaaaaag tatatacatt ttcaaccccg tttatttcat actttacaaa
     1321 aacattattt attggttttc tataaccata gtaaatgcct aaaaatttgt ccccgtcttt
     1381 cataggatat agataaagtt tttgaatctc tgatttatct aataattttt caaaaaaaat
     1441 tatacaccgc ttttgtttgt tactaactac tctatgcaat gcctttaata tttttgtgtg
     1501 atatatattt ttatcacctt ttttttcttt cagcaggaag atactttctt ttggaaattt
     1561 aattttttgt tcatttattc ttgcagcctt aagtccgatt ttcatattta atccttatat
     1621 agcctttcta tttaattctt ttaaactcaa taaacttttt tggtttttca ttagttctaa
     1681 taattcgcaa taatatgtgc aaaacacttt attgtattct attttctttt ggttattcaa
     1741 atattctttt ataattggct ttagaagttc aatatttact tcttttttta gttgttcaat
     1801 aaggatattg aaaatgtttg tttttaaatc ttgataattt tgttgagaat tttctttttt
     1861 tctttcaatt gacttctcta atttacgctt tatattgttt aaatcgctat atttatgact
     1921 ttcaataata aaatgaggct taaatttgta attttcgtat attttttgga aacttgtttc
     1981 taattgttta gaattatatt catttttttc aaattctttt tgagtgttgc atagaatttc
     2041 ctttaatttt tcttgttttt ctttcataca agatttagta aaatgtatat ttttattttt
     2101 tagtaattta atttctatta tttttatgat tttaagtatt ttgattatat tatctttatc
     2161 aatatttaaa ttgaataaaa ttgtaagaaa aattgaaaga atttttttac ataaaaagtt
     2221 acatttattg aagtaatttc ttacttgata cttttctatt tcattaatct ttctttcttc
     2281 ttttatatta tttttattat ttaaacactc ctttaaatct acactcccat ttttagtaaa
     2341 tctatcttta aggccccttg aaactctatt ttgaaatctt aaatcttttt tttcttgaaa
     2401 gtatttatag attttttggt aacattcttt ttttgaaaaa ttaagttgat agtatatttc
     2461 ggtccccaaa tttaccccta agtgtttgtg ataattagtt gtgactttaa attctttttc
     2521 taatttgtaa aggtaatttt gcagtgtttt tagtttaacg ggtgtttgac tatttctttt
     2581 aaggttttca ttaaagtaat agagtatgtt tttttgtgtg tatttcttat atgttttatt
     2641 tatgtacgct agtgttgaga taagaactac taatttatac tgtaatttat tgtagcaagt
     2701 tgtggtttta ctggttttgt ttatattaga taagttttcc atttatagct ccttgaatac
     2761 cggatttttt atctataaag tatatatatc acaattttga tttaaaagta aattcttttt
     2821 ttaaaaatac aaaaattttt tataaattat atagaccatg atattgtatt aatactaatt
     2881 gattttaatt taaggttgaa ctaaaataaa catagttttg tagaaaataa tttttcatta
     2941 ttttgctact tgatattgga tcgtaaaaat attagggctt tacaaagttc ttttaaaaga
     3001 gaatttagta aagccctaag tcttttaata aaaaattttt tgtaaaaaag ttggcaaaaa
     3061 tagtttttgc tatatattta tatataagaa aaaattcaat actcaaggag ataaaaaatg
     3121 gaaaacttaa acaataatcc acaaaaaatt aatcaaataa ataagcaaac taattttatt
     3181 ggttttgaaa attttataga acttgataat caagttaatc aaactaaaga aaaaagtaaa
     3241 ttaagtaaga taggtaagaa attacccgga ataagcagtc aagtctagtc tagtgccggc
     3301 cggaattcta aagatctttg acagctagct cagtcctagg tataatacta gtggaagagc
     3361 caaagtcaac acttatagac aaatatgatc tttctaacct tcacgaaata gcatcagggg
     3421 gagcaccttt gtctaaggaa gtaggagagg cagttgcaaa gaggtttcat ttacccggaa
     3481 ttaggcaagg atatgggtta acagaaacca catctgcaat tttaattaca cctgaaggag
     3541 atgataaacc aggagctgtt gggaaggttg ttcctttttt tgaggctaaa gttgttgatt
     3601 tagatactgg aaagacttta ggagttaatc aaagaggcga gttatgtgta agaggaccta
     3661 tgataatgtc aggatacgta aataatccag aagctacaaa tgcattgata gataaagatg
     3721 gttggttaca ctcaggcgat atagcttatt gggacgaaga tgaacacttt tttatagttg
     3781 atagattgaa atctttgatt aagtataaag gttatcaagt agctccagca gaattggaaa
     3841 gtattttatt gcaacaccca gctcttccgt tttagagcta gaaatagcaa gttaaaataa
     3901 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tggcgcgcca
     3961 aataatgccc gtgtccctgt tgaataggga cacggttttt taacaatttt tttattccta
     4021 ggccttagtg gccggtaccc ggggatcctt attactggcc gctttctagc ctggatactt
     4081 gtctggctag ttgcattaga gagtcagcta gagccctagg gctagctgtt tgtgtattcg
     4141 gagccagtgt agtttttctt tttactaacg atacgggtaa taattgattg ccttttacag
     4201 cctgaccttg agacaattgc aaaagtctgt ccacagaggt ttgacctaat aatctaaaat
     4261 cttgtttgat agtagtaagt ggaggtatgt agcaactaga atcttctgta tcatcataac
     4321 ccacaactga aatatcagcc ccaactctaa gtccactttc agtaatagct cgcatagctc
     4381 ctaaagccat ttgatcatta gcaactaaca tagcagtagg gacaatgcct tcatttaaca
     4441 tttgcattgt ttgctgaaat cctgacatag cagaccaatc tccttctctt tctgctattg
     4501 gttgtatttg atttcttgtt aaatacttgt gccatcctgc taatctcaat ctggcactta
     4561 ctgaacttaa gggacctgca agtagtgcaa tttgttgatg gcctaatgca acaaggtgtt
     4621 caactcctaa ccttgttcca tcttcgtgag aaaaaattat agagtttatt ggggtttggt
     4681 ccgacacatc caaaaacaat gctggaacat tagtacatgc tgcttcaact gcaatcgcat
     4741 cttggtcatc aagaggataa ttaattataa gtccactaac acgttgagct aataaattat
     4801 gtactgctgc cttacaagcc tctacacctg atctctcaac cattgataca acaacgcttg
     4861 caccaagttg atcagctctc gatttaattg cggctacaat ttgactaggc gcatgtaatg
     4921 caagtgatga tgtggcaact ccaattaaaa gtgattgttt ccccgcaagt tgctgagcaa
     4981 cgcgatttgg tatataattc aactctgcca tagctgcttc aaccttctct cttgtttttg
     5041 cagatacatg acttgcttgg ttcacaactc tcgaaaccgt ttggtagcta accccggcat
     5101 attcagcaac atcatacaaa gtaacaggtt tcatggatca ttcctccatg ataaaattta
     5161 aatttctgac tttggcaaat ccttttgcct gttaaaaaga atagcacaaa aattaaaaaa
     5221 aaagaaacgc aaatagcaat agcattaaaa ataattatta ttatttattt ctttgattaa
     5281 aatcaaaagc ctttaatgta ttttcttgat ctttcatgcg cttaacggta aaatccaagg
     5341 tattaagttc accttccact tttctctctc tatcttctct agaaaatcat aaaaaattta
     5401 tttgctttgt gagcggataa caattataat agattcaatt gtgagcggat aacaatttca
     5461 cacagaattc attaaagagg agaaattaca tatggataag aaatactcaa taggcttagc
     5521 tatcggcaca aatagcgtcg gatgggcggt gatcactgat gaatataagg ttccgtctaa
     5581 aaagttcaag gttctgggaa atacagaccg ccacagtatc aaaaaaaatc ttataggggc
     5641 tcttttattt gacagtggag agacagcgga agcgactcgt ctcaaacgga cagctcgtag
     5701 aaggtataca cgtcggaaga atcgtatttg ttatctacag gagatttttt caaatgagat
     5761 ggcgaaagta gatgatagtt tctttcatcg acttgaagag tcttttttgg tggaagaaga
     5821 caagaagcat gaacgtcatc ctatttttgg aaatatagta gatgaagttg cttatcatga
     5881 gaaatatcca actatctatc atctgcgaaa aaaattggta gattctactg ataaagcgga
     5941 tttgcgctta atctatttgg ccttagcgca tatgattaag tttcgtggtc attttttgat
     6001 tgagggagat ttaaatcctg ataatagtga tgtggacaaa ctatttatcc agttggtaca
     6061 aacctacaat caattatttg aagaaaaccc tattaacgca agtggagtag atgctaaagc
     6121 gattctttct gcacgattga gtaaatcaag acgattagaa aatctcattg ctcagctccc
     6181 cggtgagaag aaaaatggct tatttgggaa tctcattgct ttgtcattgg gtttgacccc
     6241 taattttaaa tcaaattttg atttggcaga agatgctaaa ttacagcttt caaaagatac
     6301 ttacgatgat gatttagata atttattggc gcaaattgga gatcaatatg ctgatttgtt
     6361 tttggcagct aagaatttat cagatgctat tttactttca gatatcctaa gagtaaatac
     6421 tgaaataact aaggctcccc tatcagcttc aatgattaaa cgctacgatg aacatcatca
     6481 agacttgact cttttaaaag ctttagttcg acaacaactt ccagaaaagt ataaagaaat
     6541 cttttttgat caatcaaaaa acggatatgc aggttatatt gatgggggag ctagccaaga
     6601 agaattttat aaatttatca aaccaatttt agaaaaaatg gatggtactg aggaattatt
     6661 ggtgaaacta aatcgtgaag atttgctgcg caagcaacgg acctttgaca acggctctat
     6721 tccccatcaa attcacttgg gtgagctgca tgctattttg agaagacaag aagactttta
     6781 tccattttta aaagacaatc gtgagaagat tgaaaaaatc ttgacttttc gaattcctta
     6841 ttatgttggt ccattggcgc gtggcaatag tcgttttgca tggatgactc ggaagtctga
     6901 agaaacaatt accccatgga attttgaaga agttgtcgat aaaggtgctt cagctcaatc
     6961 atttattgaa cgcatgacaa actttgataa aaatcttcca aatgaaaaag tactaccaaa
     7021 acatagtttg ctttatgagt attttacggt ttataacgaa ttgacaaagg tcaaatatgt
     7081 tactgaagga atgcgaaaac cagcatttct ttcaggtgaa cagaagaaag ccattgttga
     7141 tttactcttc aaaacaaatc gaaaagtaac cgttaagcaa ttaaaagaag attatttcaa
     7201 aaaaatagaa tgttttgata gtgttgaaat ttcaggagtt gaagatagat ttaatgcttc
     7261 attaggtacc taccatgatt tgctaaaaat tattaaagat aaagattttt tggataatga
     7321 agaaaatgaa gatatcttag aggatattgt tttaacattg accttatttg aagataggga
     7381 gatgattgag gaaagactta aaacatatgc tcacctcttt gatgataagg tgatgaaaca
     7441 gcttaaacgt cgccgttata ctggttgggg acgtttgtct cgaaaattga ttaatggtat
     7501 tagggataag caatctggca aaacaatatt agattttttg aaatcagatg gttttgccaa
     7561 tcgcaatttt atgcagctga tccatgatga tagtttgaca tttaaagaag acattcaaaa
     7621 agcacaagtg tctggacaag gcgatagttt acatgaacat attgcaaatt tagctggtag
     7681 ccctgctatt aaaaaaggta ttttacagac tgtaaaagtt gttgatgaat tggtcaaagt
     7741 aatggggcgg cataagccag aaaatatcgt tattgaaatg gcacgtgaaa atcagacaac
     7801 tcaaaagggc cagaaaaatt cgcgagagcg tatgaaacga atcgaagaag gtatcaaaga
     7861 attaggaagt cagattctta aagagcatcc tgttgaaaat actcaattgc aaaatgaaaa
     7921 gctctatctc tattatctcc aaaatggaag agacatgtat gtggaccaag aattagatat
     7981 taatcgttta agtgattatg atgtcgatgc cattgttcca caaagtttcc ttaaagacga
     8041 ttcaatagac aataaggtct taacgcgttc tgataaaaat cgtggtaaat cggataacgt
     8101 tccaagtgaa gaagtagtca aaaagatgaa aaactattgg agacaacttc taaacgccaa
     8161 gttaatcact caacgtaagt ttgataattt aacgaaagct gaacgtggag gtttgagtga
     8221 acttgataaa gctggtttta tcaaacgcca attggttgaa actcgccaaa tcactaagca
     8281 tgtggcacaa attttggata gtcgcatgaa tactaaatac gatgaaaatg ataaacttat
     8341 tcgagaggtt aaagtgatta ccttaaaatc taaattagtt tctgacttcc gaaaagattt
     8401 ccaattctat aaagtacgtg agattaacaa ttaccatcat gcccatgatg cgtatctaaa
     8461 tgccgtcgtt ggaactgctt tgattaagaa atatccaaaa cttgaatcgg agtttgtcta
     8521 tggtgattat aaagtttatg atgttcgtaa aatgattgct aagtctgagc aagaaatagg
     8581 caaagcaacc gcaaaatatt tcttttactc taatatcatg aacttcttca aaacagaaat
     8641 tacacttgca aatggagaga ttcgcaaacg ccctctaatc gaaactaatg gggaaactgg
     8701 agaaattgtc tgggataaag ggcgagattt tgccacagtg cgcaaagtat tgtccatgcc
     8761 ccaagtcaat attgtcaaga aaacagaagt acagacaggc ggattctcca aggagtcaat
     8821 tttaccaaaa agaaattcgg acaagcttat tgctcgtaaa aaagactggg atccaaaaaa
     8881 atatggtggt tttgatagtc caacggtagc ttattcagtc ctagtggttg ctaaggtgga
     8941 aaaagggaaa tcgaagaagt taaaatccgt taaagagtta ctagggatca caattatgga
     9001 aagaagttcc tttgaaaaaa atccgattga ctttttagaa gctaaaggat ataaggaagt
     9061 taaaaaagac ttaatcatta aactacctaa atatagtctt tttgagttag aaaacggtcg
     9121 taaacggatg ctggctagtg ccggagaatt acaaaaagga aatgagctgg ctctgccaag
     9181 caaatatgtg aattttttat atttagctag tcattatgaa aagttgaagg gtagtccaga
     9241 agataacgaa caaaaacaat tgtttgtgga gcagcataag cattatttag atgagattat
     9301 tgagcaaatc agtgaatttt ctaagcgtgt tattttagca gatgccaatt tagataaagt
     9361 tcttagtgca tataacaaac atagagacaa accaatacgt gaacaagcag aaaatattat
     9421 tcatttattt acgttgacga atcttggagc tcccgctgct tttaaatatt ttgatacaac
     9481 aattgatcgt aaacgatata cgtctacaaa agaagtttta gatgccactc ttatccatca
     9541 atccatcact ggtctttatg aaacacgcat tgatttgagt cagctaggag gtgacacgcg
     9601 tttggagcaa gtgaagttct ggagggataa aaattaataa aagcttgatc agatctcagc
     9661 ttttttttga agtgcctggc agtaagttga aaaaatttga aataaacttg gactatgtta
     9721 ataaaaaaat acaaattaac gaacaaaaag atttgaatta ttatataatt tagttaaaag
     9781 caattttaaa tgagggaggt ttccatatga gggaagcggt gatcgccgaa gtatcgactc
     9841 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     9901 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
     9961 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
    10021 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
    10081 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
    10141 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
    10201 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
    10261 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
    10321 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
    10381 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
    10441 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
    10501 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact
    10561 acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg acgtcgccgg agcggtcgag
    10621 ttctggaccg accggctcgg gttctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    10681 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    10741 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    10801 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    10861 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    10921 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    10981 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    11041 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    11101 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    11161 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    11221 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    11281 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    11341 tgggcttttg ctggcctttt gctcacatgt tctttcctgc actaga
//
