LOCUS       sequence-292457-        9622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human JARID2 in insect cells, , under a C3-cleavable 
            (PreScission Protease) N-terminal hexahistidine-MBP tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9622)
  AUTHORS   Zhang Q, McKenzie NJ, Warneford-Thomson R, Gail EH, Flanigan SF, 
            Owen BM, Lauman R, Levina V, Garcia BA, Schittenhelm RB, Bonasio R, 
            Davidovich C
  TITLE     RNA exploits an exposed regulatory site to inhibit the enzymatic 
            activity of PRC2.
  JOURNAL   Nat Struct Mol Biol. 2019 Mar;26(3):237-247. doi: 
            10.1038/s41594-019-0197-y. Epub 2019 Mar 4.
  PUBMED    30833789
REFERENCE   2  (bases 1 to 9622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-019-0197-y"; journalName: "Nat Struct Mol Biol"; 
            date: "2019-03"; volume: "26"; issue: "3"; pages: "237-247"
COMMENT     Sequence Label: pFB1.HMBP.PrS.JARID2
FEATURES             Location/Qualifiers
     source          1..9622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        198..289
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     214..231
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             350..367
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             374..1471
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="KTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEE
                     KFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLI
                     AYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA
                     DGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGET
                     AMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLE
                     NYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFW
                     YAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1328..1337
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1445..1468
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1508..1531
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     RBS             2788..2796
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    5438..5572
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5475..5494)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5529..5548
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5601..5766
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      5950..6404
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6037..6056)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6246..6267
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6430..6534
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6535..6603,6604..7395)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6753..6772)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7566..8154
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8055..8074
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8297..8314
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  8448..8672
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(8739..9272)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(9442..9447,9448..9464,9465..9470))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 acggcaaggt gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt
       61 cgcggcgctt gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg
      121 aaggcgagca tcgtttgttc gcccaggact ctagctatag ttctagtggt tggctacata
      181 ctccggaata ttaatagatc atggagataa ttaaaatgat aaccatctcg caaataaata
      241 agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata ttccggatta
      301 ttcataccgt cccaccatcg ggcgcggatc tcggtccgaa accatgggtc atcaccatca
      361 tcatcacggt accaaaactg aagaaggtaa actggtaatc tggattaacg gcgataaagg
      421 ctataacggt ctcgctgaag tcggtaagaa attcgagaaa gataccggaa ttaaagtcac
      481 cgttgagcat ccggataaac tggaagagaa attcccacag gttgcggcaa ctggcgatgg
      541 ccctgacatt atcttctggg cacacgaccg ctttggtggc tacgctcaat ctggcctgtt
      601 ggctgaaatc accccggaca aagcgttcca ggacaagctg tatccgttta cctgggatgc
      661 cgtacgttac aacggcaagc tgattgctta cccgatcgct gttgaagcgt tatcgctgat
      721 ttataacaaa gatctgctgc cgaacccgcc aaaaacctgg gaagagatcc cggcgctgga
      781 taaagaactg aaagcgaaag gtaagagcgc gctgatgttc aacctgcaag aaccgtactt
      841 cacctggccg ctgattgctg ctgacggggg ttatgcgttc aagtatgaaa acggcaagta
      901 cgacattaaa gacgtgggcg tggataacgc tggcgcgaaa gcgggtctga ccttcctggt
      961 tgacctgatt aaaaacaaac acatgaatgc agacaccgat tactccatcg cagaagctgc
     1021 ctttaataaa ggcgaaacag cgatgaccat caacggcccg tgggcatggt ccaacatcga
     1081 caccagcaaa gtgaattatg gtgtaacggt actgccgacc ttcaagggtc aaccatccaa
     1141 accgttcgtt ggcgtgctga gcgcaggtat taacgccgcc agtccgaaca aagagctggc
     1201 aaaagagttc ctcgaaaact atctgctgac tgatgaaggt ctggaagcgg ttaataaaga
     1261 caaaccgctg ggtgccgtag cgctgaagtc ttacgaggaa gagttggcga aagatccacg
     1321 tattgccgcc accatggaaa acgcccagaa aggtgaaatc atgccgaaca tcccgcagat
     1381 gtccgctttc tggtatgccg tgcgtactgc ggtgatcaac gccgccagcg gtcgtcagac
     1441 tgtcgatgaa gccctgaaag acgcgcagac tgcggccgca ggcaccgatt acgatatccc
     1501 aacgaccctg gaagttctgt tccaggggcc cgggagcaag gaaagaccca agaggaatat
     1561 cattcagaag aaatacgatg acagtgatgg gattccgtgg tcagaagaac gggtggtacg
     1621 taaagtcctt tatttgtctc tgaaggagtt caagaattcc cagaagaggc agcatgcgga
     1681 aggcattgct gggagcctga aaactgtgaa tgggctcctt ggtaatgacc agtctaaggg
     1741 attaggacca gcatcagaac agtcagagaa tgaaaaggac gatgcatccc aagtgtcctc
     1801 cactagcaac gatgttagtt cttcagattt tgaagaaggg ccgtcgagga aaaggcccag
     1861 gctgcaagca caaaggaagt ttgctcagtc tcagccgaat agtcccagca caactccagt
     1921 aaagatagtg gagccattgc taccccctcc agctactcag atatcagacc tctctaaaag
     1981 gaagcctaag acagaagatt ttcttacctt tctctgcctt cgaggttctc ctgcgctgcc
     2041 caacagcatg gtgtattttg gaagctctca ggatgaggag gaagtcgagg aggaagatga
     2101 tgagacagaa gacgtcaaaa cagccaccaa caatgcttca tcttcatgcc agtcgacccc
     2161 caggaaagga aaaacccaca aacatgttca caacgggcat gttttcaatg gttccagcag
     2221 gtcaacacgg gagaaggaac ctgttcaaaa acacaaaagc aaagaggcca ctcccgcaaa
     2281 ggagaagcac agcgatcacc gggctgacag ccgccgggag caggcttcag ctaaccaccc
     2341 cgcagcggcc ccctccacgg gttcctcggc caaggggctt gctgccaccc atcaccaccc
     2401 ccctctgcat cggtcggctc aggacttacg gaaacaggtt tctaaggtaa acggagtcac
     2461 tcgaatgtca tctctgggtg caggtgtaac cagtgccaaa aagatgcgcg aggtcagacc
     2521 ttcaccatcc aaaactgtga agtacactgc cacggtgacg aagggggctg tcacatacac
     2581 caaagccaag agagaactgg tcaaggacac caaacccaat caccacaagc ccagttccgc
     2641 tgtcaaccac acaatctcag ggaaaactga aagtagcaat gcaaaaaccc gcaaacaggt
     2701 gctatccctc gggggggcgt ccaagtccac tgggcccgcc gtcaatggcc tcaaggtcag
     2761 tggcaggttg aacccaaagt catgcactaa ggaggtgggg gggcggcagc tgcgggaggg
     2821 cctgcagctg cgggaggggc tgcggaactc caagaggaga ctggaagagg cacaccaggc
     2881 ggagaagccg cagtcgcccc ccaagaagat gaaaggggcg gctggccccg ccgaaggccc
     2941 tggcaagaag gccccggccg agagaggtct gctgaacgga cacgtgaaga aggaagtgcc
     3001 ggagcgcagt ctggagagga atcggccgaa gcgggccacg gccgggaaga gcacgccagg
     3061 cagacaagca catggcaagg cggacagcgc ctcctgtgaa aatcgttcta cctcgcaacc
     3121 ggagtccgtg cacaagccgc aggactcggg caaggccgag aagggcggcg gcaaggccgg
     3181 gtgggcggcc atggacgaga tccccgtcct caggccctcc gccaaggagt tccacgatcc
     3241 gctcatctac atcgagtcgg tccgcgctca ggtggagaag ttcgggatgt gcagggtgat
     3301 cccccctccg gactggcggc ccgagtgcaa gctcaacgat gagatgcggt ttgtcacgca
     3361 gattcagcac atccacaagc tgggccggcg ctggggcccc aacgtgcagc ggctggcctg
     3421 catcaagaag cacctcaaat ctcagggcat caccatggac gagctcccgc tcataggggg
     3481 ctgtgagctc gacctggcct gctttttccg gctgattaat gagatgggcg gcatgcagca
     3541 agtgactgac ctcaaaaaat ggaacaaact agcagacatg ctgcgcatcc ccagaactgc
     3601 ccaggaccgg ctggccaagc tgcaggaggc ctactgccag tacctactct cctacgactc
     3661 cctgtcccca gaggagcacc ggcggctgga gaaggaggtg ctgatggaga aggagatcct
     3721 ggagaagcgc aaggggccgc tggaaggcca cacagagaac gaccaccaca agttccaccc
     3781 tctgccccgc ttcgagccca agaatgggct catccacggc gtggccccca ggaacggctt
     3841 ccgcagcaag ctcaaggagg tgggccaggc ccagttgaag actggccggc ggcgactctt
     3901 cgctcaggaa aaagaagtgg tcaaggaaga ggaggaggac aaaggcgtcc tcaatgactt
     3961 ccacaagtgc atctataagg gaaggtctgt ttctctaaca actttttatc gaacagcgag
     4021 gaatatcatg agcatgtgtt tcagcaagga gcctgcccca gccgaaatcg agcaagagta
     4081 ctggaggcta gtggaagaga aggactgcca cgtggcagtg cactgcggca aggtggacac
     4141 caacactcac ggcagtggat tcccagtagg aaaatcagaa cccttttcga ggcatggatg
     4201 gaacctcacc gtcctcccca ataacacagg gtccatcctg cgtcacctcg gtgctgtgcc
     4261 tggagtgact attccctggc taaatattgg catggtcttt tctacctcat gctggtctcg
     4321 agaccaaaat caccttccat acattgacta cttacacact ggtgctgact gcatttggta
     4381 ttgcattcct gctgaggagg agaacaagct ggaagatgtg gtccacaccc tgctgcaagc
     4441 caatggcacc ccagggctgc agatgctgga aagcaacgtc atgatctccc cggaggtgct
     4501 gtgcaaagag gggatcaagg tgcacaggac cgtgcagcag agtggccagt ttgtcgtctg
     4561 cttcccggga tcctttgtgt ccaaagtgtg ctgtgggtac agcgtgtctg aaaccgtgca
     4621 ctttgctacc acccagtgga caagtatggg ctttgagacc gccaaggaaa tgaagcgtcg
     4681 ccatatagct aagccattct ccatggagaa gttactctac cagattgcac aagcagaagc
     4741 aaaaaaagaa aacggtccca ctctcagtac catctcagcc ctcctggatg agctcaggga
     4801 tacagagctg cggcagcgca ggcagctgtt cgaggctggc ctccactcct ccgcacgcta
     4861 tggcagccac gatggcagca gcacggtggc ggacgggaag aaaaagcctc gaaagtggct
     4921 gcagttggag acgtcagaga ggaggtgtca gatctgccag cacctgtgct acctgtccat
     4981 ggtggtacaa gagaacgaaa acgtcgtgtt ctgtctggag tgtgctctgc gccacgtgga
     5041 gaaacagaag tcctgccgag ggctgaagtt gatgtaccgc tacgatgagg aacagattat
     5101 cagtctggtc aatcagatct gcggcaaagt gtctggtaaa aacggcagca ttgagaactg
     5161 tctcagtaaa cccacaccaa aaagaggtcc ccgcaagaga gcgacagtgg acgtgccccc
     5221 ctcccgtctg tcagcctcca gttcatccaa aagtgcttcg agctcatcat gataatctag
     5281 agcctgcagt ctcgaggcat gcggtaccaa gcttgtcgag aagtactaga ggatcataat
     5341 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     5401 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     5461 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5521 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatcact
     5581 gatatcgcct aggagatccg aaccagataa gtgaaatcta gttccaaact attttgtcat
     5641 ttttaatttt cgtattagct tacgacgcta cacccagttc ccatctattt tgtcactctt
     5701 ccctaaataa tccttaaaaa ctccatttcc acccctccca gttcccaact attttgtccg
     5761 cccacagcgg ggcatttttc ttcctgttat gtttttaatc aaacatcctg ccaactccat
     5821 gtgacaaacc gtcatcttcg gctacttttt ctctgtcaca gaatgaaaat ttttctgtca
     5881 tctcttcgtt attaatgttt gtaattgact gaatatcaac gcttatttgc agcctgaatg
     5941 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     6001 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     6061 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     6121 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     6181 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     6241 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     6301 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     6361 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     6421 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     6481 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     6541 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     6601 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     6661 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     6721 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     6781 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     6841 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     6901 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     6961 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     7021 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     7081 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     7141 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     7201 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     7261 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     7321 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     7381 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     7441 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     7501 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     7561 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7621 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7681 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     7741 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7801 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7861 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7921 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     7981 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     8041 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     8101 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8161 agcaacgcgg cctttttacg gttcctggcc ttttgctcac atgttctttc ctgcgttatc
     8221 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     8281 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     8341 ttttctcctt acgcatctgt gcggtatttc acaccgcata gaccagccgc gtaacctggc
     8401 aaaatcggtt acggttgagt aataaatgga tgccctgcgt aagcgggtgt gggcggacaa
     8461 taaagtctta aactgaacaa aatagatcta aactatgaca ataaagtctt aaactagaca
     8521 gaatagttgt aaactgaaat cagtccagtt atgctgtgaa aaagcatact ggacttttgt
     8581 tatggctaaa gcaaactctt cattttctga agtgcaaatt gcccgtcgta ttaaagaggg
     8641 gcgtggccaa gggcatggta aagactatat tcgcggcgtt gtgacaattt accgaacaac
     8701 tccgcggccg ggaagccgat ctcggcttga acgaattgtt aggtggcggt acttgggtcg
     8761 atatcaaagt gcatcacttc ttcccgtatg cccaactttg tatagagagc cactgcggga
     8821 tcgtcaccgt aatctgcttg cacgtagatc acataagcac caagcgcgtt ggcctcatgc
     8881 ttgaggagat tgatgagcgc ggtggcaatg ccctgcctcc ggtgctcgcc ggagactgcg
     8941 agatcataga tatagatctc actacgcggc tgctcaaact tgggcagaac gtaagccgcg
     9001 agagcgccaa caaccgcttc ttggtcgaag gcagcaagcg cgatgaatgt cttactacgg
     9061 agcaagttcc cgaggtaatc ggagtccggc tgatgttggg agtaggtggc tacgtctccg
     9121 aactcacgac cgaaaagatc aagagcagcc cgcatggatt tgacttggtc agggccgagc
     9181 ctacatgtgc gaatgatgcc catacttgag ccacctaact ttgttttagg gcgactgccc
     9241 tgctgcgtaa catcgttgct gctgcgtaac atcaaacatc gacccacggc gtaacgcgct
     9301 tgctgcttgg atgcccgagg catagactgt acaaaaaaac agtcataaca agccatgaaa
     9361 accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag
     9421 cgcatacgct acttgcatta cagtttacga accgaacagg cttatgtcaa ctgggttcgt
     9481 gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag cgaagtcgag
     9541 gcatttctgt cctggctggc gaacgagcgc aaggtttcgg tctccacgca tcgtcaggca
     9601 ttggcggcct tgctgttctt ct
//
