LOCUS       sequence-292464-       10983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (L156P 
            mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10983)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 10983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-myc-HLA-A2-VN (L156P)
FEATURES             Location/Qualifiers
     source          1..10983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(724..742)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        810..914
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(915..983,984..1775)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1133..1152)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1946..2534
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2435..2454
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2965..3110
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3153..4190
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4188..4207)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4232..4280
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4934..5313
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5314..5517
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5468..5488
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5514..5538
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5620..5649
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(6697..6699,6700..7158)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYLTA"
     primer_bind     complement(6739..6760)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7000..7019)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    7181..7315
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7205..7224)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7259..7278
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7458..7480)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ctcccgctcc tgctcctgct cctgttccac cgtgggtccc tttgcagcca atgcaacttg
       61 gacgtttttg gggtctccgg acaccatctc tatgtcttgg ccctgatcct gagccgcccg
      121 gggctcctgg tcttccgcct cctcgtcctc gtcctcttcc ccgtcctcgt ccatggttat
      181 caccccctct tctttgaggt ccactgccgc cggagccttc tggtccagat gtgtctccct
      241 tctctcctag gccatttcca ggtcctgtac ctggcccctc gtcagacatg attcacacta
      301 aaagagatca atagacatct ttattagacg acgctcagtg aatacaggga gtgcagactc
      361 ctgccccctc caacagcccc cccaccctca tccccttcat ggtcgctgtc agacagatcc
      421 aggtctgaaa attccccatc ctccgaacca tcctcgtcct catcaccaat tactcgcagc
      481 ccggaaaact cccgctgaac atcctcaaga tttgcgtcct gagcctcaag ccaggcctca
      541 aattcctcgt cccccttttt gctggacggt agggatgggg attctcggga cccctcctct
      601 tcctcttcaa ggtcaccaga cagagatgct actggggcaa cggaagaaaa gctgggtgcg
      661 gcctgtgagg atcagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa
      721 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
      781 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
      841 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
      901 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
      961 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     1021 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     1081 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     1141 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
     1201 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     1261 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     1321 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     1381 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     1441 gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac
     1501 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     1561 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     1621 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     1681 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     1741 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     1801 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     1861 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     1921 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     1981 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2041 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     2101 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2161 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2221 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2281 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2341 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2401 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2461 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2521 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     2581 cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg
     2641 gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg
     2701 tcgcgaacgc cagcaagacg tagcccagcg cgtcggcccc gagatgcgcc gcgtgcggct
     2761 gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt
     2821 tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg
     2881 ccctgcttca tccccgtggc ccgttgctcg cgtttgctgg cggtgtcccc ggaagaaata
     2941 tatttgcatg tctttagttc tatgatgaca caaaccccgc ccagcgtctt gtcattggcg
     3001 aattcgaaca cgcagatgca gtcggggcgg cgcggtccga ggtccacttc gcatattaag
     3061 gtgacgcgtg tggcctcgaa caccgagcga ccctgcagcg acccgcttaa cagcgtcaac
     3121 agcgtgccgc agatcccggg gggcaatgag atatgaaaaa gcctgaactc accgcgacgt
     3181 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
     3241 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
     3301 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg
     3361 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt
     3421 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg
     3481 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga
     3541 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt
     3601 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg
     3661 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg
     3721 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc
     3781 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg
     3841 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg
     3901 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg
     3961 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
     4021 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg
     4081 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa
     4141 accgacgccc cagcactcgt ccggatcggg agatggggga ggctaactga aacacggaag
     4201 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
     4261 gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
     4321 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
     4381 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
     4441 catagccact ggccccgtgg gttagggacg gggtccccca tggggaatgg tttatggttc
     4501 gtgggggtta ttattttggg cgttgcgtgg ggtcaggtcc acgactggac tgagcagaca
     4561 gacccatggt ttttggatgg cctgggcatg gaccgcatgt actggcgcga cacgaacacc
     4621 gggcgtctgt ggctgccaaa cacccccgac ccccaaaaac caccgcgcgg atttctggcg
     4681 tgccaagcta gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg
     4741 ttgttgccat tgctgcaggc gcagaactgg taggtatgga agatctatac attgaatcaa
     4801 tattggcaat tagccatatt agtcattggt tatatagcat aaatcaatat tggctattgg
     4861 ccattgcata cgttgtatct atatcataat atgtacattt atattggctc atgtccaata
     4921 tgaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     4981 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     5041 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     5101 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     5161 ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt
     5221 aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag
     5281 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat
     5341 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     5401 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc
     5461 ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
     5521 ttagtgaacc gtcagatctc tagaagctgg gtaccagctg ctagcgccac catgaagacc
     5581 atcatcgccc tgagctacat cttctgcctg gtgttcgccg agcagaaatt gatcagcgag
     5641 gaggacctgg gcagccctgg aggcagttca ggaggcggat ctcactccat gaggtatttc
     5701 ttcacatccg tgtcccggcc cggccgcggg gagccccgct tcatcgcagt gggctacgtg
     5761 gacgacacgc agttcgtgcg gttcgacagc gacgccgcga gccagaggat ggagccgcgg
     5821 gcgccgtgga tagagcagga gggtccggag tattgggacg gggagacacg gaaagtgaag
     5881 gcccactcac agactcaccg agtggacctg gggaccctgc gcggctacta caaccagagc
     5941 gaggccggtt ctcacaccgt ccagaggatg tatggctgcg acgtggggtc ggactggcgc
     6001 ttcctccgcg ggtaccacca gtacgcctac gacggcaagg attacatcgc cctgaaagag
     6061 gacctgcgct cttggaccgc ggcggacatg gcagctcaga ccaccaagca caagtgggag
     6121 gcggcccatg tggcggagca gcctagagcc tacctggagg gcacgtgcgt ggagtggctc
     6181 cgcagatacc tggagaacgg gaaggagacg ctgcagcgta cggacgcccc caaaacgcat
     6241 atgactcacc acgctgtctc tgaccatgaa gccaccctga ggtgctgggc cctgagcttc
     6301 taccctgcgg agatcacact gacctggcag cgggatgggg aggaccagac ccaggacacg
     6361 gagctcgtgg agaccaggcc tgcaggggat ggaaccttcc agaagtgggc ggctgtggtg
     6421 gtgccttctg gacaggagca gagatacacc tgccatgtgc agcatgaggg tttgcccaag
     6481 cccctcaccc tgagatggga gccgtcttcc cagcccacca tccccatcgt gggcatcatt
     6541 gctggcctgg ttctctttgg agctgtgatc actggagctg tggtcgctgc tgtgatgtgg
     6601 aggaggaaga gctcagatag aaaaggaggg agctactctc aggctgcaag cagtgacagt
     6661 gcccagggct ctgatgtgtc tctcacagct tgtaaagtga gcaagggcga ggagctgttc
     6721 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     6781 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc
     6841 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcctg
     6901 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     6961 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     7021 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     7081 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     7141 aacgtctatc tgaccgccta actcgaggcc ggcaaggccg gatccagaca tgataagata
     7201 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     7261 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     7321 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     7381 caagtaaaac ctctacaaat gtggtatggc tgattatgat ccggctgcct cgcgcgtttc
     7441 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     7501 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     7561 cggggcgcag ccatgaggtc gactctagag gatcgatccc cgccccggac gaactaaacc
     7621 tgactacgac atctctgccc cttcttcgcg gggcagtgca tgtaatccct tcagttggtt
     7681 ggtacaactt gccaactggg ccctgttcca catgtgacac ggggggggac caaacacaaa
     7741 ggggttctct gactgtagtt gacatcctta taaatggatg tgcacatttg ccaacactga
     7801 gtggctttca tcctggagca gactttgcag tctgtggact gcaacacaac attgccttta
     7861 tgtgtaactc ttggctgaag ctcttacacc aatgctgggg gacatgtacc tcccaggggc
     7921 ccaggaagac tacgggaggc tacaccaacg tcaatcagag gggcctgtgt agctaccgat
     7981 aagcggaccc tcaagagggc attagcaata gtgtttataa ggcccccttg ttaaccctaa
     8041 acgggtagca tatgcttccc gggtagtagt atatactatc cagactaacc ctaattcaat
     8101 agcatatgtt acccaacggg aagcatatgc tatcgaatta gggttagtaa aagggtccta
     8161 aggaacagcg atatctccca ccccatgagc tgtcacggtt ttatttacat ggggtcagga
     8221 ttccacgagg gtagtgaacc attttagtca caagggcagt ggctgaagat caaggagcgg
     8281 gcagtgaact ctcctgaatc ttcgcctgct tcttcattct ccttcgttta gctaatagaa
     8341 taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt
     8401 gacgccccca gaataaaatt tggacggggg gttcagtggt ggcattgtgc tatgacacca
     8461 atataaccct cacaaacccc ttgggcaata aatactagtg taggaatgaa acattctgaa
     8521 tatctttaac aatagaaatc catggggtgg ggacaagccg taaagactgg atgtccatct
     8581 cacacgaatt tatggctatg ggcaacacat aatcctagtg caatatgata ctggggttat
     8641 taagatgtgt cccaggcagg gaccaagaca ggtgaaccat gttgttacac tctatttgta
     8701 acaaggggaa agagagtgga cgccgacagc agcggactcc actggttgtc tctaacaccc
     8761 ccgaaaatta aacggggctc cacgccaatg gggcccataa acaaagacaa gtggccactc
     8821 ttttttttga aattgtggag tgggggcacg cgtcagcccc cacacgccgc cctgcggttt
     8881 tggactgtaa aataagggtg taataacttg gctgattgta accccgctaa ccactgcggt
     8941 caaaccactt gcccacaaaa ccactaatgg caccccgggg aatacctgca taagtaggtg
     9001 ggcgggccaa gataggggcg cgattgctgc gatctggagg acaaattaca cacacttgcg
     9061 cctgagcgcc aagcacaggg ttgttggtcc tcatattcac gaggtcgctg agagcacggt
     9121 gggctaatgt tgccatgggt agcatatact acccaaatat ctggatagca tatgctatcc
     9181 taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
     9241 taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc
     9301 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
     9361 taatmkrkat tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc
     9421 aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     9481 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     9541 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
     9601 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     9661 ctgtatccgg gtagcatatg ctatcctcat gcatatacag tcagcatatg atacccagta
     9721 gtagagtggg agtgctatcc tttgcatatg ccgccacctc ccaagggggc gtgaattttc
     9781 gctgcttgtc cttttcctgc tggttgctcc cattcttagg tgaatttaag gaggccaggc
     9841 taaagccgtc gcatgtctga ttgctcacca ggtaaatgtc gctaatgttt tccaacgcga
     9901 gaaggtgttg agcgcggagc tgagtgacgt gacaacatgg gtatgcccaa ttgccccatg
     9961 ttgggaggac gaaaatggtg acaagacaga tggccagaaa tacaccaaca gcacgcatga
    10021 tgtctactgg ggatttattc tttagtgcgg gggaatacac ggcttttaat acgattgagg
    10081 gcgtctccta acaagttaca tcactcctgc ccttcctcac cctcatctcc atcacctcct
    10141 tcatctccgt catctccgtc atcaccctcc gcggcagccc cttccaccat aggtggaaac
    10201 cagggaggca aatctactcc atcgtcaaag ctgcacacag tcaccctgat attgcaggta
    10261 ggagcgggct ttgtcataac aaggtcctta atcgcatcct tcaaaacctc agcaaatata
    10321 tgagtttgta aaaagaccat gaaataacag acaatggact cccttagcgg gccaggttgt
    10381 gggccgggtc caggggccat tccaaagggg agacgactca atggtgtaag acgacattgt
    10441 ggaatagcaa gggcagttcc tcgccttagg ttgtaaaggg aggtcttact acctccatat
    10501 acgaacacac cggcgaccca agttccttcg tcggtagtcc tttctacgtg actcctagcc
    10561 aggagagctc ttaaaccttc tgcaatgttc tcaaatttcg ggttggaacc tccttgacca
    10621 cgatgctttc caaaccaccc tccttttttg cgcctgcctc catcaccctg accccggggt
    10681 ccagtgcttg ggccttctcc tgggtcatct gcggggccct gctctatcgc tcccgggggc
    10741 acgtcaggct caccatctgg gccaccttct tggtggtatt caaaataatc ggcttcccct
    10801 acagggtgga aaaatggcct tctacctgga gggggcctgc gcggtggaga cccggatgat
    10861 gatgactgac tactgggact cctgggcctc ttttctccac gtccacgacc tctccccctg
    10921 gctctttcac gacttccccc cctggctctt tcacgtcctc taccccggcg gcctccacta
    10981 cct
//
