LOCUS       sequence-292501-        1194 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For the FRET-based binding assay between human CNNM3 CBS domain and 
            PRL2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Cai F, Huang Y, Wang M, Sun M, Zhao Y, Hattori M
  TITLE     A FRET-based screening method to detect potential inhibitors of the 
            binding of CNNM3 to PRL2.
  JOURNAL   Sci Rep. 2020 Jul 30;10(1):12879. doi: 10.1038/s41598-020-69818-x.
  PUBMED    32733084
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-07-30"; volume: "10(1):12879. doi"; pages: " 
            10.1038/s41598-020-69818-x"
COMMENT     Sequence Label: CNNM3-CBS-YPet
FEATURES             Location/Qualifiers
     source          1..1194
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             475..1188
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLE
                     FLTAAGITEGMNELYK"
ORIGIN
        1 catatgtaca gcgatctcag caagggcgtg ctgagatgca ggacagtgga ggacgtgctc
       61 acccccctgg aggactgttt catgctggac gccagcaccg tgctcgactt tggcgtgctc
      121 gcctccatca tgcagtccgg ccacacccgt attcctgtgt atgaggagga gaggtccaat
      181 atcgtggaca tgctctacct gaaggacctg gccttcgtgg accctgagga ctgcacacct
      241 ctgagcacca tcaccaggtt ctacaaccac cccctccact tcgtgttcaa tgacaccaaa
      301 ctggacgccg tgctggagga gttcaagagg ggcaagagcc acctggccat cgtgcagaag
      361 gtcaacaatg agggcgaggg agaccccttc tacgaggtgc tgggcctggt gaccctggag
      421 gacgtcatcg aggagatcat caggtccgag atcctggacg agctcgaggg aagtatgtct
      481 aaaggtgaag aattattcac tggtgttgtc ccaattttgg ttgaattaga tggtgatgtt
      541 aatggtcaca aattttctgt ctccggtgaa ggtgaaggtg atgctacgta cggtaaattg
      601 accttaaaat tactctgtac tactggtaaa ttgccagttc catggccaac cttagtcact
      661 actttaggtt atggtgttca atgttttgct agatacccag atcatatgaa acaacatgac
      721 tttttcaagt ctgccatgcc agaaggttat gttcaagaaa gaactatttt tttcaaagat
      781 gacggtaact acaagaccag agctgaagtc aagtttgaag gtgatacctt agttaataga
      841 atcgaattaa aaggtattga ttttaaagaa gatggtaaca ttttaggtca caaattggaa
      901 tacaactata actctcacaa tgtttacatc actgctgaca aacaaaagaa tggtatcaaa
      961 gctaacttca aaattagaca caacattgaa gatggtggtg ttcaattagc tgaccattat
     1021 caacaaaata ctccaattgg tgatggtcca gtcttgttac cagacaacca ttacttatcc
     1081 tatcaatctg ccttattcaa agatccaaac gaaaagagag accacatggt cttgttagaa
     1141 tttttgactg ctgctggtat taccgagggt atgaatgaat tgtacaaact cgag
//
