LOCUS       sequence-292512-        1958 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression plasmid for SARS-CoV-2 E (envelope).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1958)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046814
FEATURES             Location/Qualifiers
     source          1..1958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    43..67
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             82..104
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             337..357
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             358..375
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     rep_origin      496..1084
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     985..1004
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1190..1849
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1256..1275)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1920..1954
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 catcgatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa
       61 caattcccct ctagaaataa ttttgtttaa ctttaagaag gagactcttc tatgtatagt
      121 tttgtatcag aggaaacggg gacacttatt gtcaatagcg tgttgctgtt cctagctttc
      181 gttgtttttc tgctcgtgac cttagcgatc ctgactgcct tgcgtctgtg tgcatactgc
      241 tgcaacatcg ttaacgtatc cctggtaaag ccgtctttct acgtgtattc gcgcgttaaa
      301 aacctcaaca gctctagggt tcccgacctg cttgttgaga acctgtactt tcaaagccac
      361 catcatcacc atcattaacc ggcttatcgg tcagtttcac ctgatttacg taaaaacccg
      421 cttcggcggg tttttgcttt tggaggggca gaaagatgaa tgactgtcca cgacgctata
      481 cccaaaagaa aaagcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
      541 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
      601 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
      661 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
      721 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
      781 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
      841 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
      901 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
      961 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1021 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1081 gaaattgatc gggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc
     1141 gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa
     1201 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
     1261 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
     1321 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
     1381 ccgcctgatg aatgctcatc cggaatttcg tatggcaatg aaagacggtg agctggtgat
     1441 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
     1501 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
     1561 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
     1621 cgtttcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
     1681 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct
     1741 gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat
     1801 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttgatatcga
     1861 gctcgcttgg actcctgttg atagatccag taatgacctc agaactccat ctggatttgt
     1921 tcagaacgct cggttgccgc cgggcgtttt ttattggt
//
