LOCUS       sequence-292518-       14404 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ABEmax, EBNA1 and blasticidin resistence gene; cloning 
            backbone for sgRNA; Contains Epstein-Barr virus oriP replication 
            origin .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 14404)
  AUTHORS   Qi T, Wu F, Xie Y, Gao S, Li M, Pu J, Li D, Lan F, Wang Y
  TITLE     Base Editing Mediated Generation of Point Mutations Into Human 
            Pluripotent Stem Cells for Modeling Disease.
  JOURNAL   Front Cell Dev Biol. 2020 Sep 25;8:590581. doi: 
            10.3389/fcell.2020.590581. eCollection 2020.
  PUBMED    33102492
REFERENCE   2  (bases 1 to 14404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Dev Biol."; date: "2020-09-25"; volume: "8:590581. doi"; pages: " 
            10.3389/fcell.2020.590581. eCollection 2020"
COMMENT     Sequence Label: epi-ABEmax
FEATURES             Location/Qualifiers
     source          1..14404
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(872..890)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        958..1062
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1063..1131,1132..1923)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1281..1300)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2094..2682
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2583..2602
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2952..3265
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     3184..3204
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_RNA        3286..3361
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    3409..3526
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3595..3806
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             3868..3888
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5077..5100,5101..5103,5104..9177)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5077..9177
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9220..9240
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(9245..9262)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9251..9475
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        9523..9852
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9703..9838
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9874..10272
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    10438..10572
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10475..10494)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10529..10548
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11212..13001
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ctcccccggc ggcctccact acctcctcga ccccggcctc cactacctcc tcgaccccgg
       61 cctccactgc ctcctcgacc ccggcctcca cctcctgctc ctgcccctcc tgctcctgcc
      121 cctcctcctg ctcctgcccc tcctgcccct cctgctcctg ctcctgctcc tgttccaccg
      181 tgggtccctt tgcagccaat gcaacttgga cgtttttggg gtctccggac accatctcta
      241 tgtcttggcc ctgatcctga gccgcccggg gctcctggtc ttccgcctcc tcgtcctcgt
      301 cctcttcccc gtcctcgtcc atggttatca ccccctcttc tttgaggtcc actgccgccg
      361 gagccttctg gtccagatgt gtctcccttc tctcctaggc catttccagg tcctgtacct
      421 ggcccctcgt cagacatgat tcacactaaa agagatcaat agacatcttt attagacgac
      481 gctcagtgaa tacagggagt gcagactcct gccccctcca acagcccccc caccctcatc
      541 cccttcatgg tcgctgtcag acagatccag gtctgaaaat tccccatcct ccgaaccatc
      601 ctcgtcctca tcaccaatta ctcgcagccc ggaaaactcc cgctgaacat cctcaagatt
      661 tgcgtcctga gcctcaagcc aggcctcaaa ttcctcgtcc ccctttttgc tggacggtag
      721 ggatggggat tctcgggacc cctcctcttc ctcttcaagg tcaccagaca gagatgctac
      781 tggggcaacg gaagaaaagc tgggtgcggc ctgtgaggat cagcttatcg atgataagct
      841 gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
      901 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
      961 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1021 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1081 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1141 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     1201 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     1261 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     1321 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     1381 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     1441 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     1501 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     1561 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca
     1621 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     1681 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     1741 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     1801 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     1861 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     1921 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     1981 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2041 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2101 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2161 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2221 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     2281 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2341 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2401 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2461 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2521 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2581 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2641 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     2701 tttttacggt tcctggcctt ttgctggcct tgaagctgtc cctgatggtc gtcatctacc
     2761 tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc
     2821 ataatgggga aggccatcca gcctcgcgtc gaactagatg atccggctgt ggaatgtgtg
     2881 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     2941 ttatgtctag agatccgacg ccgccatctc taggcccgcg ccggccccct cgcacagact
     3001 tgtgggagaa gctcggctac tcccctgccc cggttaattt gcatataata tttcctagta
     3061 actatagagg cttaatgtgc gataaaagac agataatctg ttctttttaa tactagctac
     3121 attttacatg ataggcttgg atttctataa gagatacaaa tactaaatta ttattttaaa
     3181 aaacagcaca aaaggaaact caccctaact gtaaagtaat tgtgtgtttt gagactataa
     3241 atatgcatgc gagaaaagcc ttgtttagaa gagcgatgct cttcggtttt agagctagaa
     3301 atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
     3361 cttttttgaa ttctagatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa
     3421 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     3481 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     3541 ggacagcaga gatccacttt ggcgccggct cgagtggctc cggtgcccgt cagtgggcag
     3601 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
     3661 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     3721 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     3781 gcaacgggtt tgccgccaga acacaggtgt cgtgacgcgg gatccgccac catgaaacgg
     3841 acagccgacg gaagcgagtt cgagtcacca aagaagaagc ggaaagtctc tgaagtcgag
     3901 tttagccacg agtattggat gaggcacgca ctgaccctgg caaagcgagc atgggatgaa
     3961 agagaagtcc ccgtgggcgc cgtgctggtg cacaacaata gagtgatcgg agagggatgg
     4021 aacaggccaa tcggccgcca cgaccctacc gcacacgcag agatcatggc actgaggcag
     4081 ggaggcctgg tcatgcagaa ttaccgcctg atcgatgcca ccctgtatgt gacactggag
     4141 ccatgcgtga tgtgcgcagg agcaatgatc cacagcagga tcggaagagt ggtgttcgga
     4201 gcacgggacg ccaagaccgg cgcagcaggc tccctgatgg atgtgctgca ccaccccggc
     4261 atgaaccacc gggtggagat cacagaggga atcctggcag acgagtgcgc cgccctgctg
     4321 agcgatttct ttagaatgcg gagacaggag atcaaggccc agaagaaggc acagagctcc
     4381 accgactctg gaggatctag cggaggatcc tctggaagcg agacaccagg cacaagcgag
     4441 tccgccacac cagagagctc cggcggctcc tccggaggat cctctgaggt ggagttttcc
     4501 cacgagtact ggatgagaca tgccctgacc ctggccaaga gggcacgcga tgagagggag
     4561 gtgcctgtgg gagccgtgct ggtgctgaac aatagagtga tcggcgaggg ctggaacaga
     4621 gccatcggcc tgcacgaccc aacagcccat gccgaaatta tggccctgag acagggcggc
     4681 ctggtcatgc agaactacag actgattgac gccaccctgt acgtgacatt cgagccttgc
     4741 gtgatgtgcg ccggcgccat gatccactct aggatcggcc gcgtggtgtt tggcgtgagg
     4801 aacgcaaaaa ccggcgccgc aggctccctg atggacgtgc tgcactaccc cggcatgaat
     4861 caccgcgtcg aaattaccga gggaatcctg gcagatgaat gtgccgccct gctgtgctat
     4921 ttctttcgga tgcctagaca ggtgttcaat gctcagaaga aggcccagag ctccaccgac
     4981 tccggaggat ctagcggagg ctcctctggc tctgagacac ctggcacaag cgagagcgca
     5041 acacctgaaa gcagcggggg cagcagcggg gggtcagaca agaagtacag catcggcctg
     5101 gccatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc
     5161 aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa cctgatcgga
     5221 gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag aaccgccaga
     5281 agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt cagcaacgag
     5341 atggccaagg tggacgacag cttcttccac agactggaag agtccttcct ggtggaagag
     5401 gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt ggcctaccac
     5461 gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac cgacaaggcc
     5521 gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg ccacttcctg
     5581 atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat ccagctggtg
     5641 cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag
     5701 gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat cgcccagctg
     5761 cccggcgaga agaagaatgg cctgttcgga aacctgattg ccctgagcct gggcctgacc
     5821 cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct gagcaaggac
     5881 acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta cgccgacctg
     5941 tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct gagagtgaac
     6001 accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga cgagcaccac
     6061 caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa gtacaaagag
     6121 attttcttcg accagagcaa gaacggctac gccggctaca ttgacggcgg agccagccag
     6181 gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac cgaggaactg
     6241 ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga caacggcagc
     6301 atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca ggaagatttt
     6361 tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc
     6421 tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac cagaaagagc
     6481 gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc ttccgcccag
     6541 agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa ggtgctgccc
     6601 aagcacagcc tgctgtacga gtacttcacc gtgtataacg agctgaccaa agtgaaatac
     6661 gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg
     6721 gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga ggactacttc
     6781 aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg gttcaacgcc
     6841 tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt cctggacaat
     6901 gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt tgaggacaga
     6961 gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa agtgatgaag
     7021 cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct gatcaacggc
     7081 atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga cggcttcgcc
     7141 aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga ggacatccag
     7201 aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa tctggccggc
     7261 agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga gctcgtgaaa
     7321 gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga gaaccagacc
     7381 acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa
     7441 gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct gcagaacgag
     7501 aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca ggaactggac
     7561 atcaaccggc tgtccgacta cgatgtggac catatcgtgc ctcagagctt tctgaaggac
     7621 gactccatcg acaacaaggt gctgaccaga agcgacaaga accggggcaa gagcgacaac
     7681 gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcggcagct gctgaacgcc
     7741 aagctgatta cccagagaaa gttcgacaat ctgaccaagg ccgagagagg cggcctgagc
     7801 gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca gatcacaaag
     7861 cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa tgacaagctg
     7921 atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt ccggaaggat
     7981 ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga cgcctacctg
     8041 aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag cgagttcgtg
     8101 tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc
     8161 ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt caagaccgag
     8221 attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa cggcgaaacc
     8281 ggggagatcg tgtgggataa gggccgggat tttgccaccg tgcggaaagt gctgagcatg
     8341 ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag caaagagtct
     8401 atcctgccca agaggaacag cgataagctg atcgccagaa agaaggactg ggaccctaag
     8461 aagtacggcg gcttcgacag ccccaccgtg gcctattctg tgctggtggt ggccaaagtg
     8521 gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat caccatcatg
     8581 gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg ctacaaagaa
     8641 gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct ggaaaacggc
     8701 cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc
     8761 tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa gggctccccc
     8821 gaggataatg agcagaaaca gctgtttgtg gaacagcaca agcactacct ggacgagatc
     8881 atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa
     8941 gtgctgtccg cctacaacaa gcaccgggat aagcccatca gagagcaggc cgagaatatc
     9001 atccacctgt ttaccctgac caatctggga gcccctgccg ccttcaagta ctttgacacc
     9061 accatcgacc ggaagaggta caccagcacc aaagaggtgc tggacgccac cctgatccac
     9121 cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg aggtgactct
     9181 ggcggctcaa aaagaaccgc cgacggcagc gaattcgagc ccaagaagaa gaggaaagtc
     9241 taagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     9301 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     9361 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     9421 ggggaggatt gggaagagaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     9481 gaggcggaaa gaaccagctg gggctcgata ccgctgtgga atgtgtgtca gttagggtgt
     9541 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     9601 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     9661 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     9721 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     9781 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     9841 ggcttttgca aaaagctccc gggagcttgg ataatggcca agcctttgtc tcaagaagaa
     9901 tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac
     9961 agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat
    10021 cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca
    10081 gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc
    10141 tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag
    10201 gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg
    10261 tgggagggct aagaattctt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
    10321 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
    10381 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
    10441 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
    10501 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    10561 catgtctgga tcccttgaaa tacatccaca ggtacacctc caattgactc aaatgatgtc
    10621 aattagtcta tcagaagctt gtggaaggct ggagacggtc acagcttgtc tgtaagcgga
    10681 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
    10741 agccatgagg tcgactctag aggatcgatc cccgccccgg acgaactaaa cctgactacg
    10801 acatctctgc cccttcttcg cggggcagtg catgtaatcc cttcagttgg ttggtacaac
    10861 ttgccaactg ggccctgttc cacatgtgac acgggggggg accaaacaca aaggggttct
    10921 ctgactgtag ttgacatcct tataaatgga tgtgcacatt tgccaacact gagtggcttt
    10981 catcctggag cagactttgc agtctgtgga ctgcaacaca acattgcctt tatgtgtaac
    11041 tcttggctga agctcttaca ccaatgctgg gggacatgta cctcccaggg gcccaggaag
    11101 actacgggag gctacaccaa cgtcaatcag aggggcctgt gtagctaccg ataagcggac
    11161 cctcaagagg gcattagcaa tagtgtttat aaggccccct tgttaaccct aaacgggtag
    11221 catatgcttc ccgggtagta gtatatacta tccagactaa ccctaattca atagcatatg
    11281 ttacccaacg ggaagcatat gctatcgaat tagggttagt aaaagggtcc taaggaacag
    11341 cgatatctcc caccccatga gctgtcacgg ttttatttac atggggtcag gattccacga
    11401 gggtagtgaa ccattttagt cacaagggca gtggctgaag atcaaggagc gggcagtgaa
    11461 ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt tagctaatag aataactgct
    11521 gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc
    11581 cagaataaaa tttggacggg gggttcagtg gtggcattgt gctatgacac caatataacc
    11641 ctcacaaacc ccttgggcaa taaatactag tgtaggaatg aaacattctg aatatcttta
    11701 acaatagaaa tccatggggt ggggacaagc cgtaaagact ggatgtccat ctcacacgaa
    11761 tttatggcta tgggcaacac ataatcctag tgcaatatga tactggggtt attaagatgt
    11821 gtcccaggca gggaccaaga caggtgaacc atgttgttac actctatttg taacaagggg
    11881 aaagagagtg gacgccgaca gcagcggact ccactggttg tctctaacac ccccgaaaat
    11941 taaacggggc tccacgccaa tggggcccat aaacaaagac aagtggccac tctttttttt
    12001 gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc gccctgcggt tttggactgt
    12061 aaaataaggg tgtaataact tggctgattg taaccccgct aaccactgcg gtcaaaccac
    12121 ttgcccacaa aaccactaat ggcaccccgg ggaatacctg cataagtagg tgggcgggcc
    12181 aagatagggg cgcgattgct gcgatctgga ggacaaatta cacacacttg cgcctgagcg
    12241 ccaagcacag ggttgttggt cctcatattc acgaggtcgc tgagagcacg gtgggctaat
    12301 gttgccatgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
    12361 atctgggtag cataggctat cctaatctat atctgggtag tatatgctat cctaatctgt
    12421 atccgggtag catatgctat cctaatagag attagggtag catatgctat cctaatctat
    12481 atctgggtag cataggctat cctaatctat atctgggtag tatatgctat cctaatctgt
    12541 atccgggtag catatgctat cctaatagag attagggtag tatatgctat cctaatttat
    12601 atctgggtag catatactac ccaaatatct ggatagcata tgctatccta atctatatct
    12661 gggtagcata tgctatccta atctatatct gggtagcata ggctatccta atctatatct
    12721 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atttatatct
    12781 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
    12841 gggtagtata tgctatccta atctgtatcc gggtagcata tgctatcctc atgcatatac
    12901 agtcagcata tgatacccag tagtagagtg ggagtgctat cctttgcata tgccgccacc
    12961 tcccaagggg gcgtgaattt tcgctgcttg tccttttcct gctggttgct cccattctta
    13021 ggtgaattta aggaggccag gctaaagccg tcgcatgtct gattgctcac caggtaaatg
    13081 tcgctaatgt tttccaacgc gagaaggtgt tgagcgcgga gctgagtgac gtgacaacat
    13141 gggtatgccc aattgcccca tgttgggagg acgaaaatgg tgacaagaca gatggccaga
    13201 aatacaccaa cagcacgcat gatgtctact ggggatttat tctttagtgc gggggaatac
    13261 acggctttta atacgattga gggcgtctcc taacaagtta catcactcct gcccttcctc
    13321 accctcatct ccatcacctc cttcatctcc gtcatctccg tcatcaccct ccgcggcagc
    13381 cccttccacc ataggtggaa accagggagg caaatctact ccatcgtcaa agctgcacac
    13441 agtcaccctg atattgcagg taggagcggg ctttgtcata acaaggtcct taatcgcatc
    13501 cttcaaaacc tcagcaaata tatgagtttg taaaaagacc atgaaataac agacaatgga
    13561 ctcccttagc gggccaggtt gtgggccggg tccaggggcc attccaaagg ggagacgact
    13621 caatggtgta agacgacatt gtggaatagc aagggcagtt cctcgcctta ggttgtaaag
    13681 ggaggtctta ctacctccat atacgaacac accggcgacc caagttcctt cgtcggtagt
    13741 cctttctacg tgactcctag ccaggagagc tcttaaacct tctgcaatgt tctcaaattt
    13801 cgggttggaa cctccttgac cacgatgctt tccaaaccac cctccttttt tgcgcctgcc
    13861 tccatcaccc tgaccccggg gtccagtgct tgggccttct cctgggtcat ctgcggggcc
    13921 ctgctctatc gctcccgggg gcacgtcagg ctcaccatct gggccacctt cttggtggta
    13981 ttcaaaataa tcggcttccc ctacagggtg gaaaaatggc cttctacctg gagggggcct
    14041 gcgcggtgga gacccggatg atgatgactg actactggga ctcctgggcc tcttttctcc
    14101 acgtccacga cctctccccc tggctctttc acgacttccc cccctggctc tttcacgtcc
    14161 tctaccccgg cggcctccac tacctcctcg accccggcct ccactacctc ctcgaccccg
    14221 gcctccactg cctcctcgac cccggcctcc acctcctgct cctgcccctc ctgctcctgc
    14281 ccctcctcct gctcctgccc ctcctgcccc tcctgctcct gctcctgctc ctgttccacc
    14341 gtgggtccct ttgcagccaa tgcaacttgg acgtttttgg ggtctccgga caccatctct
    14401 atgt
//
