LOCUS       sequence-292551-        4992 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast Expression plasmid for SARS-CoV-2 ORF6 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4992)
  TITLE     SARS-CoV-2 protein Yeast expression constructs, tagged, for protein 
            expression and/or purification
REFERENCE   2  (bases 1 to 4992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046311
FEATURES             Location/Qualifiers
     source          1..4992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        45..486
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    45..162
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     457..480
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             681..698
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             699..722
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(767..785)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             1136..1939
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      2022..3004
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     terminator      3082..3111
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      3133..3720
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(3732..3759)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(3783..3974)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(3975..4766,4767..4835))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4598..4617
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4836..4938)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(4939..4970)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 gatttaaatg gtcagttaga gcggccgctt gctatattga agtacggatt agaagccgcc
       61 gagcgggcga cagccctccg acggaagact ctcctccgtg cgtcctcgtc ttcaccggtc
      121 gcgttcctga aacgcagatg tgcctcgcgc cgcactgctc cgaacaataa agattctaca
      181 atactagctt ttatggttat gaagaggaaa aattggcagt aacctggccc cacaaacctt
      241 caaattaacg aatcaaatta acaaccatag gatgataatg cgattagttt tttagcctta
      301 tttctggggt aattaatcag cgaagcgatg atttttgatc tattaacaga tatataaatg
      361 gaaaagctgc ataaccactt taactaatac tttcaacatt ttcagtttgt attacttctt
      421 attcaaatgt cataaaagta tcaacaaaaa attgttaata tacctctata ctttaacgtc
      481 aaggagaaaa aactataatg tttcatctcg ttgactttca ggttactata gcagagatat
      541 tactaattat tatgaggact tttaaagttt ccatttggaa tcttgattac atcataaacc
      601 tcataattaa aaatttatct aagtcactaa ctgagaataa atattctcaa ttagatgaag
      661 agcaaccaat ggagattgat ctcgtgccga gaggttcttg gagccatccg cagtttgaaa
      721 aataaacagg ccccttttcc tttgtcgata tcatgtaatt agttatgtca cgcttacatt
      781 cacgccctcc tcccacatcc gctctaaccg aaaaggaagg agttagacaa cctgaagtct
      841 aggtccctat ttattttttt taatagttat gttagtatta agaacgttat ttatatttca
      901 aatttttctt ttttttctgt acaaagcggc cgccgaacca ctagtgcact gcagtacacg
      961 gtttccttga aatttttttg attcggtaat ctccgaacag aaggaagaac gaaggaagga
     1021 gcacagactt agattggtat atatacgcat atgtagtgtt gaagaaacat gaaattgccc
     1081 agtattctta acccaactgc acagaacaaa aacgtgcagg aaacgaagat aaatcatgtc
     1141 gaaagctaca tataaggaac gtgctgctac tcatcctagt cctgttgctg ccaagctatt
     1201 taatatcatg cacgaaaagc aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa
     1261 ggaattactg gagttagttg aagcattagg tcccaaaatt tgtttactaa aaacacatgt
     1321 ggatatcttg actgattttt ccatggaggg cacagttaag ccgctaaagg cattatccgc
     1381 caagtacaat tttttactct tcgaggacag aaaatttgct gacattggta atacagtcaa
     1441 attgcagtac tctgcgggtg tatacagaat agcagaatgg gcagacatta cgaatgcaca
     1501 cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag gcggcagaag aagtaacaaa
     1561 ggaacctaga ggccttttga tgttagcaga attgtcatgc aagggctccc tatctactgg
     1621 agaatatact aagggtactg ttgacattgc gaagagcgac aaagattttg ttatcggctt
     1681 tattgctcaa agagacatgg gtggaagaga tgaaggttac gattggttga ttatgacacc
     1741 cggtgtgggt ttagatgaca agggagatgc attgggtcaa cagtatagaa ccgtggatga
     1801 tgtggtttct acaggatctg acattattat tgttggaaga ggactatttg caaagggaag
     1861 ggatgctaag gtagagggtg aacgttacag aaaagcaggc tgggaagcat atttgagaag
     1921 atgcggccag caaaactaaa aaactgtatt ataagtaaat gcatgtatac taaactcaca
     1981 aattagagct tcaatttaat tatatcagtt attacccgag taacgaagca tctgtgcttc
     2041 attttgtaga acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct
     2101 gcatttttac agaacagaaa tgcaacgcga aagcgctatt ttaccaacga agaatctgtg
     2161 cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct
     2221 gagctgcatt tttacagaac agaaatgcaa cgcgagagcg ctattttacc aacaaagaat
     2281 ctatacttct tttttgttct acaaaaatgc atcccgagag cgctattttt ctaacaaagc
     2341 atcttagatt actttttttc tcctttgtgc gctctataat gcagtctctt gataactttt
     2401 tgcactgtag gtccgttaag gttagaagaa ggctactttg gtgtctattt tctcttccat
     2461 aaaaaaagcc tgactccact tcccgcgttt actgattact agcgaagctg cgggtgcatt
     2521 ttttcaagat aaaggcatcc ccgattatat tctataccga tgtggattgc gcatactttg
     2581 tgaacagaaa gtgatagcgt tgatgattct tcattggtca gaaaattatg aacggtttct
     2641 tctattttgt ctctatatac tacgtatagg aaatgtttac attttcgtat tgttttcgat
     2701 tcactctatg aatagttctt actacaattt ttttgtctaa agagtaatac tagagataaa
     2761 cataaaaaat gtagaggtcg agtttagatg caagttcaag gagcgaaagg tggatgggta
     2821 ggttatatag ggatatagca cagagatata tagcaaagag atacttttga gcaatgtttg
     2881 tggaagcggt attcgcaata ttttagtagc tcgttacagt ccggtgcgtt tttggttttt
     2941 tgaaagtgcg tcatcagagc gcttttggtt ttcaaaagcg ctctgaagtt cctatacttt
     3001 ctagctagag aataggaact tcccgagcgg ccgcgtgtta caagcttgac tccagcgtaa
     3061 ctggactgca atcaactcac tggctcacct tcacgggtgg gcctttcttc ggtagaaaat
     3121 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3181 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgag
     3241 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     3301 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3361 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3421 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3481 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3541 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3601 cgcacgaggg agcttccagg ggtaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3661 cacctctgac ttgagcatcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3721 aacgccagca acgcagaaag gcccacccga aggtgagcca ggtgattaca tttgggccct
     3781 catcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca
     3841 gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt
     3901 ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt
     3961 tcctgtttgg tcacttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     4021 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat gcgggagggc
     4081 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     4141 ttatcagcaa taaaccagcc agccgggagg gccgagcgca gaagtgatcc tgcaacttta
     4201 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     4261 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     4321 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     4381 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgccagaag taagttggcc
     4441 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     4501 gtgagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     4561 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     4621 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gtaaactctc aaggatctta
     4681 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     4741 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     4801 ggaataaggg cgacacggaa atgttgaata ctcattttag cttccttagc tcctgaaaat
     4861 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     4921 cctcttacgt gccgatcaag tcaaaagcct ccggtcggag gcttttgact ttctgctatg
     4981 gaggtcaggt at
//
