LOCUS       sequence-292562-       17261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing VirD-Cas9 fusion under ubiquitin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17261)
  AUTHORS   Ali Z, Shami A, Sedeek K, Kamel R, Alhabsi A, Tehseen M, Hassan N, 
            Butt H, Kababji A, Hamdan SM, Mahfouz MM
  TITLE     Fusion of the Cas9 endonuclease and the VirD2 relaxase facilitates 
            homology-directed repair for precise genome engineering in rice.
  JOURNAL   Commun Biol. 2020 Jan 23;3(1):44. doi: 10.1038/s42003-020-0768-9.
  PUBMED    31974493
REFERENCE   2  (bases 1 to 17261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-01-23"; volume: "3(1):44. doi"; pages: " 
            10.1038/s42003-020-0768-9"
COMMENT     Sequence Label: pRGEB-VirD2-Cas9
FEATURES             Location/Qualifiers
     source          1..17261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             26..49
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      71..323
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(347..363)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(364..380)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(373..395)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    583..607
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1908..2537
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2966..4039
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      4105..4299
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4535..4557)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4643..4783
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4698..4717
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4798..4815)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4969..5557)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5049..5068)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5644..6438)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6863..6887
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    6965..7139
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(7180..8205)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(8272..8948)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(8315..8335)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    9139..9160
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9175..9180,9181..9198,9199..9205)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9213..9229
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9218..9240
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9237..9253
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9237..9253
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        9271..9651
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     misc_RNA        9668..9743
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        9824..11533
                     /gene="RUBQ2"
                     /label=Ubi promoter
                     /note="promoter for the rice (Oryza sativa) polyubiquitin 
                     gene RUBQ2"
     intron          10572..11533
                     /note="intron upstream of the coding sequence for the rice 
                     polyubiquitin gene RUBQ2"
     protein_bind    11544..11568
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             11613..11678
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             11685..11705
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             13071..17171
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             13071..17171
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             17172..17219
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     protein_bind    complement(join(17252..17261,1..15))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cttgtacaaa gtggttgata acagcgacta caaggatgac gatgacaagg cttagagctc
       61 gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
      121 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
      181 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
      241 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
      301 ggtgtcatct atgttactag atcggggtaa ccgaacgcgt gaattcactg gccgtcgttt
      361 tacactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
      421 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
      481 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgc tagagcagct tgagcttgga
      541 tcagattgtc gtttcccgcc ttcagtttaa actatcagtg tttgacagga tatattggcg
      601 ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa
      661 aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca
      721 aagtactttg atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca
      781 gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct
      841 gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga
      901 ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc
      961 ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg
     1021 ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc
     1081 caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct
     1141 ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg
     1201 cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt
     1261 gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag
     1321 cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc
     1381 ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc
     1441 ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga
     1501 agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc
     1561 cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag
     1621 gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc
     1681 gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc
     1741 ggtttgtctg atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag
     1801 cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct
     1861 gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg
     1921 ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg
     1981 ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc
     2041 gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga
     2101 cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc
     2161 cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg
     2221 tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc
     2281 gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag
     2341 gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg
     2401 agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc
     2461 ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta
     2521 aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct
     2581 aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga
     2641 cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa
     2701 gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca
     2761 gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag
     2821 gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgttgag
     2881 gaacgggcgg ttggccaggc gtaagcggct gggttgcctg ccggccctgc aatggcactg
     2941 gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat
     3001 cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg
     3061 gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat
     3121 ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa
     3181 gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag
     3241 tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga
     3301 ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat
     3361 ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat
     3421 gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc
     3481 ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga
     3541 aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa
     3601 cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa
     3661 gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga
     3721 gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga
     3781 tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc
     3841 cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt
     3901 ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga
     3961 ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga
     4021 agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga
     4081 aaaaggtcga aaagcactct ttcctgtgaa tagcacgtac attgggaacc caaagccgta
     4141 cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca
     4201 catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa
     4261 acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc
     4321 cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc
     4381 gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct
     4441 accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac
     4501 cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     4561 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     4621 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
     4681 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     4741 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc
     4801 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     4861 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     4921 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     4981 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     5041 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     5101 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     5161 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     5221 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     5281 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     5341 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     5401 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     5461 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     5521 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     5581 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgcattctag
     5641 gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat
     5701 ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga
     5761 ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat
     5821 aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga
     5881 aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc
     5941 tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat cgttattcag
     6001 taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat
     6061 gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct
     6121 ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt
     6181 gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct ttccttccag
     6241 ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc
     6301 cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga
     6361 cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca attccggtga
     6421 tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt aaagataccc
     6481 caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt
     6541 aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg
     6601 acggagccga ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat
     6661 caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc
     6721 ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga
     6781 cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt
     6841 cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta
     6901 gacaacttaa taacacattg cggacgtttt taatgtactg aattaacgcc gaattaattc
     6961 gggggatctg gattttagta ctggattttg gttttaggaa ttagaaattt tattgataga
     7021 agtattttac aaatacaaat acatactaag ggtttcttat atgctcaaca catgagcgaa
     7081 accctatagg aaccctaatt cccttatctg ggaactactc acacattatt atggagaaac
     7141 tcgagcttgt cgatcgacag atcccggtcg gcatctactc tatttctttg ccctcggacg
     7201 agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac
     7261 ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat
     7321 tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg
     7381 atagagttgg tcaagaccaa tgcggagcat atacgcccgg agtcgtggcg atcctgcaag
     7441 ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct
     7501 ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc
     7561 aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg
     7621 cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg
     7681 cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc
     7741 agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa
     7801 tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tagcctccgc
     7861 gaccggttgt agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg
     7921 tgaacggcgg gagatgcaat aggtcaggct ctcgctaaac tccccaatgt caagcacttc
     7981 cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc
     8041 atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc
     8101 acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat
     8161 cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatatctc attgcccccc
     8221 ggatctgcga aagctcgaga gagatagatt tgtagagaga gactggtgat ttcagcgtgt
     8281 cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag gatagtggga
     8341 ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct ttgaagacgt
     8401 ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc
     8461 actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta
     8521 ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa
     8581 tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc
     8641 tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttcac
     8701 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg
     8761 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc
     8821 ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga
     8881 agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa
     8941 aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga
     9001 gagtgtcgtg ctccaccatg ttggcaagct gctctagcca atacgcaaac cgcctctccc
     9061 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     9121 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     9181 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     9241 aaacagctat gacatgatta cgccaagctt aaggaatctt taaacatacg aacagatcac
     9301 ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag
     9361 atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg
     9421 ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac
     9481 tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat
     9541 gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta
     9601 tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc aggagaccga
     9661 ggtctcggtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
     9721 aaaaagtggc accgagtcgg tgcttttttg ttttagagct agaaatagca agttaaaata
     9781 aggctagtcc gtttttagcg cgtgcatgcc tgcaggtcca caaattcggg tcaaggcgga
     9841 agccagcgcg ccaccccacg tcagcaaata cggaggcgcg gggttgacgg cgtcacccgg
     9901 tcctaacggc gaccaacaaa ccagccagaa gaaattacag taaaaaaaaa gtaaattgca
     9961 ctttgatcca ccttttatta cctaagtctc aatttggatc acccttaaac ctatcttttc
    10021 aatttgggcc gggttgtggt ttggactacc atgaacaact tttcgtcatg tctaacttcc
    10081 ctttcagcaa acatatgaac catatataga ggagatcggc cgtatactag agctgatgtg
    10141 tttaaggtcg ttgattgcac gagaaaaaaa aatccaaatc gcaacaatag caaatttatc
    10201 tggttcaaag tgaaaagata tgtttaaagg tagtccaaag taaaacttat agataataaa
    10261 atgtggtcca aagcgtaatt cactcaaaaa aaatcaacga gacgtgtacc aaacggagac
    10321 aaacggcatc ttctcgaaat ttcccaaccg ctcgctcgcc cgcctcgtct tcccggaaac
    10381 cgcggtggtt tcagcgtggc ggattctcca agcagacgga gacgtcacgg cacgggactc
    10441 ctcccaccac ccaaccgcca taaataccag ccccctcatc tcctctcctc gcatcagctc
    10501 cacccccgaa aaatttctcc ccaatctcgc gaggctctcg tcgtcgaatc gaatcctctc
    10561 gcgtcctcaa ggtacgctgc ttctcctctc ctcgcttcgt ttcgattcga tttcggacgg
    10621 gtgaggttgt tttgttgcta gatccgattg gtggttaggg ttgtcgatgt gattatcgtg
    10681 agatgtttag gggttgtaga tctgatggtt gtgatttggg cacggttggt tcgataggtg
    10741 gaatcgtggt taggttttgg gattggatgt tggttctgat gattgggggg aatttttacg
    10801 gttagatgaa ttgttggatg attcgattgg ggaaatcggt gtagatctgt tggggaattg
    10861 tggaactagt catgcctgag tgattggtgc gatttgtagc gtgttccatc ttgtaggcct
    10921 tgttgcgagc atgttcagat ctactgttcc gctcttgatt gagttattgg tgccatgggt
    10981 tggtgcaaac acaggcttta atatgttata tctgttttgt gtttgatgta gatctgtagg
    11041 gtagttcttc ttagacatgg ttcaattatg tagcttgtgc gtttcgattt gatttcatat
    11101 gttcacagat tagataatga tgaactcttt taattaattg tcaatggtaa ataggaagtc
    11161 ttgtcgctat atctgtcata atgatctcat gttactatct gccagtaatt tatgctaaga
    11221 actatattag aatatcatgt tacaatctgt agtaatatca tgttacaatc tgtagttcat
    11281 ctatataatc tattgtggta atttcttttt actatctgtg tgaagattat tgccactagt
    11341 tcattctact tatttctgaa gttcaggata cgtgtgctgt tactacctat ctgaatacat
    11401 gtgtgatgtg cctgttacta tctttttgaa tacatgtatg ttctgttgga atatgtttgc
    11461 tgtttgatcc gttgttgtgt ccttaatctt gtgctagttc ttaccctatc tgtttggtga
    11521 ttatttcttg cagatagtta tcaacaagtt tgtacaaaaa agcaggcttc gaaggagata
    11581 gaaccaattc tctaaggaaa tacttaacca tggactataa ggaccacgac ggagactaca
    11641 aggatcatga tattgattac aaagacgatg acgataagat ggccccaaag aagaagcgga
    11701 aggtcatgcc tgacagagca caggttataa ttcgtatagt accaggaggg ggaacaaaga
    11761 cgcttcaaca aatcattaat caattagaat atttatcaag gaagggtagg cttgaactac
    11821 agcgttctgc acgtcatttg gatatcccgc taccaccaga tcaaatacat gaactcgcta
    11881 gaagttgggt ccaggaaaca ggcacttatg atgagtctca acccgatgaa gaaagacaac
    11941 aagagcttac aacacatata atcgtatcat tccccgccgg aacgtcacaa gtagcagctt
    12001 atgcagcatc ccgagagtgg gcagcagaaa tgtttggatc cggagctgga ggtggccgtt
    12061 ataactatct taccgctttc cacattgacc gagatcatcc tcatctccat gttgtagtta
    12121 atagaagaga acttttagga cacggctggc ttaaaatatc tcgaagacat ccacaactta
    12181 attacgatgc tctacgaata aagatggctg aaatatcact gagacacgga atagccctag
    12241 atgcgagtag gagagccgaa aggggtatca cagaacgacc aatcacatat gcacaataca
    12301 gaagactcga aagggagcaa gcgaggcaaa ttagatttga agacgcagat ctggaacaat
    12361 caagtcctca aggagaccac ccagagttta gtcaaccttt tgacacctcc ccatttgagg
    12421 cgtctgcagg tggaccagaa gacatgccta ggccaaataa tagacagaat gaatctcaag
    12481 tccatctcca ggaacccgca ggtgttagca atgaagctgg agtgcttgtt agagtagcac
    12541 tagaaacaga aagactcgct caaccatttg tttcggaaac tatcttagcg gacgatatag
    12601 ggtcgggatc ttcacgagtc gcggaaggaa gagtcgaaag tgctaatcga acaccagata
    12661 ttccaagagc agctacagaa gcagcaaccc atactactca tgatagacag agacgtgcca
    12721 agagacctca tgacgatgac ggagggccat cgggggctaa aagagttaca ctagaaggaa
    12781 tcgcagttgg gccacaagcc aatgccggag aacaggatgg ttcctcaggt ccgcttgtca
    12841 gacaggctgg gactagcaga ccgagtcccc ctaccgcaac cacccgagct agtacagcaa
    12901 ctgattcatt atctgcaact gcgcacttac aacaacgaag aggggtattg tctaagagac
    12961 ctagagaaga tgacgatggt gaacctagtg aaaggaaaag agaaagagat gaaagatcca
    13021 aagatggaag aggagggaac agaagaggta tccacggagt cccagcagcc gacaagaagt
    13081 acagcatcgg cctggacatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt
    13141 acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga
    13201 agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga
    13261 agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga
    13321 tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct
    13381 tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg
    13441 aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca
    13501 gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc
    13561 ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt
    13621 tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg
    13681 gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc
    13741 tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg attgccctga
    13801 gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc
    13861 agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc
    13921 agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca
    13981 tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat
    14041 acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg
    14101 agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacattgacg
    14161 gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg
    14221 gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct
    14281 tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc
    14341 ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga
    14401 ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga
    14461 tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg
    14521 gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg
    14581 agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat aacgagctga
    14641 ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga
    14701 aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga
    14761 aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag
    14821 atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg
    14881 acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac
    14941 tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg
    15001 acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga
    15061 agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt
    15121 ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta
    15181 aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg
    15241 ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg
    15301 acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca
    15361 gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg
    15421 aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc
    15481 agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg
    15541 accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc gtgcctcaga
    15601 gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac aagaaccggg
    15661 gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcggc
    15721 agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc aaggccgaga
    15781 gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc
    15841 ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg
    15901 agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg
    15961 atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc
    16021 acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg
    16081 aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga
    16141 gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact
    16201 ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga
    16261 caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc accgtgcgga
    16321 aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct
    16381 tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc agaaagaagg
    16441 actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg
    16501 tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg
    16561 ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca
    16621 agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg
    16681 agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag aagggaaacg
    16741 aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc
    16801 tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaagcact
    16861 acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg
    16921 ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc atcagagagc
    16981 aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca
    17041 agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg
    17101 ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc
    17161 tgggaggcga caaaaggccg gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagt
    17221 aagaattcgc ggccgcactc gagatatcta gacccagctt t
//
