LOCUS       sequence-292565-       11844 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Acetylcholine sensor for sindbis virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11844)
  AUTHORS   Borden PM, Zhang P, Shivange AV, Marvin JS, Cichon J, Dan C, 
            Podgorski K, Figueiredo A, Novak O, Tanimoto M, Shigetomi E, Lobas 
            MA, Kim H, Zhu PK, Zhang Y, Zheng WS, Fan C, Wang G, Xiang B, Gan L,
            Zhang G, Guo K, Lin L, Cai Y, Yee AG, Aggarwal A, Ford CP, Rees DC, 
            Dietrich D, Khakh BS, Dittman JS, Gan W, Koyama M, Jayaraman V, 
            Cheer JF, Lester HA, Zhu JJ, Looger LL
  TITLE     A fast genetically encoded fluorescent sensor for faithful in vivo 
            acetylcholine detection in mice, fish, worms and flies
  JOURNAL   bioRxiv 2020
REFERENCE   2  (bases 1 to 11844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2020"
COMMENT     Sequence Label: pSinREP5-iAchSnFR
FEATURES             Location/Qualifiers
     source          1..11844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     sig_peptide     1..60
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     primer_bind     513..534
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(637..658)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             1645..1674
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1678..1824
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     promoter        2307..2411
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2412..2480,2481..3272)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2630..2649)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3443..4031
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3932..3951
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        4119..4137
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4119..4136
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt
       61 gacagatctg cgaacgacac cgtagttgtg ggctcgatca tctttacaga agggattatc
      121 gtcgcaaaca tggtggcaga gatgattgag gcgcatacag accttaaggt ggttcgcaaa
      181 ctgaaccttg gcggggtcaa cgttaacttt gaagccatta aacgcggagg tgcgaataat
      241 ggtattgaca tttacgtgga gtacactggg cacggtcttg tggatattct ggggttcccg
      301 gagccgaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc gaacttcaag
      361 atccgccaca acgtggagga cggcagcgtg cagctcgccg accactacca gcagaacacc
      421 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgtg
      481 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
      541 gccgggatca ctctcggcat ggacgagctg tacaagggcg gtaccggagg gagcatgagc
      601 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
      661 aacggccaca agttcagcgt gcgcggcgag ggcgagggcg atgccaccaa cggcaagctg
      721 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
      781 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
      841 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatcag cttcaaggac
      901 gacggcacct acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
      961 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     1021 tacaactttc cgccgcccgc gactactgat ccagaaggtg catacgaaac cgtgaagaag
     1081 gagtacaaac gtaaatggaa tattgtatgg ctcaaaccac tgggattcaa caatacgtat
     1141 acgcttaccg ttaaagacga actggcgaaa cagtataacc ttaaaacctt cagtgactta
     1201 gcgaaaatct cggataagct gattctgggt gcaacgatgt tctttttaga agggcccgat
     1261 ggttacccag gcctgcaaaa actgtacaat ttcaaattca agcacaccaa aagcatggac
     1321 atgggtattc gctataccgc cattgataat aacgaagttc aggtaattga tgcctgggcc
     1381 actgatggct tgctggtgag ccacaaatta aaaattctgg aggatgataa agcgttcttc
     1441 ccgccgtatt atgctgcccc catcatccgt caggatgtct tagataagca tcctgaactg
     1501 aaggacgtgc tgaacaaact cgcgaatcaa atttcactgg aagaaatgca gaaactgaat
     1561 tacaaggtgg acggtgaggg tcaggaccca gcgaaagtag ctaaggagtt tttgaaagag
     1621 aaaggtttaa ttctgcaggt cgacgaacaa aaactcatct cagaagagga tctgaatgct
     1681 gtgggccagg acacgcagga ggtcatcgtg gtgccacact ccttgccctt taaggtggtg
     1741 gtgatctcag ccatcctggc cctggtggtg ctcaccatca tctcccttat catcctcatc
     1801 atgctttggc agaagaagcc acgttaggcg gcgtgagcat gcaggccttg ggcccaatga
     1861 tccgaccagc aaaactcgat gtacttccga ggaactgatg tgcataatgc atcaggctgg
     1921 tacattagat ccccgcttac cgcgggcaat atagcaacac taaaaactcg atgtacttcc
     1981 gaggaagcgc agtgcataat gctgcgcagt gttgccacat aaccactata ttaaccattt
     2041 atctagcgga cgccaaaaac tcaatgtatt tctgaggaag cgtggtgcat aatgccacgc
     2101 agcgtctgca taacttttat tatttctttt attaatcaac aaaattttgt ttttaacatt
     2161 tcaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaagg ggaattcctc gataattaag
     2221 cggccgctcg agcggccgct taattaatcg aggggaatta attcttgaag acgaaagggc
     2281 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     2341 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     2401 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     2461 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     2521 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     2581 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     2641 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     2701 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     2761 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     2821 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     2881 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     2941 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     3001 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     3061 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     3121 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     3181 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     3241 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     3301 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     3361 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     3421 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3481 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     3541 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     3601 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     3661 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     3721 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     3781 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     3841 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     3901 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     3961 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4021 gcctatggaa aaacgccagc aacgcgagct cgtatggaca tattgtcgtt agaacgcggc
     4081 tacaattaat acataacctt atgtatcata cacatacgat ttaggtgaca ctatagattg
     4141 acggcgtagt acacactatt gaatcaaaca gccgaccaat tgcactacca tcacaatgga
     4201 gaagccagta gtaaacgtag acgtagaccc ccagagtccg tttgtcgtgc aactgcaaaa
     4261 aagcttcccg caatttgagg tagtagcaca gcaggtcact ccaaatgacc atgctaatgc
     4321 cagagcattt tcgcatctgg ccagtaaact aatcgagctg gaggttccta ccacagcgac
     4381 gatcttggac ataggcagcg caccggctcg tagaatgttt tccgagcacc agtatcattg
     4441 tgtctgcccc atgcgtagtc cagaagaccc ggaccgcatg atgaaatatg ccagtaaact
     4501 ggcggaaaaa gcgtgcaaga ttacaaacaa gaacttgcat gagaagatta aggatctccg
     4561 gaccgtactt gatacgccgg atgctgaaac accatcgctc tgctttcaca acgatgttac
     4621 ctgcaacatg cgtgccgaat attccgtcat gcaggacgtg tatatcaacg ctcccggaac
     4681 tatctatcat caggctatga aaggcgtgcg gaccctgtac tggattggct tcgacaccac
     4741 ccagttcatg ttctcggcta tggcaggttc gtaccctgcg tacaacacca actgggccga
     4801 cgagaaagtc cttgaagcgc gtaacatcgg actttgcagc acaaagctga gtgaaggtag
     4861 gacaggaaaa ttgtcgataa tgaggaagaa ggagttgaag cccgggtcgc gggtttattt
     4921 ctccgtagga tcgacacttt atccagaaca cagagccagc ttgcagagct ggcatcttcc
     4981 atcggtgttc cacttgaatg gaaagcagtc gtacacttgc cgctgtgata cagtggtgag
     5041 ttgcgaaggc tacgtagtga agaaaatcac catcagtccc gggatcacgg gagaaaccgt
     5101 gggatacgcg gttacacaca atagcgaggg cttcttgcta tgcaaagtta ctgacacagt
     5161 aaaaggagaa cgggtatcgt tccctgtgtg cacgtacatc ccggccacca tatgcgatca
     5221 gatgactggt ataatggcca cggatatatc acctgacgat gcacaaaaac ttctggttgg
     5281 gctcaaccag cgaattgtca ttaacggtag gactaacagg aacaccaaca ccatgcaaaa
     5341 ttaccttctg ccgatcatag cacaagggtt cagcaaatgg gctaaggagc gcaaggatga
     5401 tcttgataac gagaaaatgc tgggtactag agaacgcaag cttacgtatg gctgcttgtg
     5461 ggcgtttcgc actaagaaag tacattcgtt ttatcgccca cctggaacgc agacctgcgt
     5521 aaaagtccca gcctctttta gcgcttttcc catgtcgtcc gtatggacga cctctttgcc
     5581 catgtcgctg aggcagaaat tgaaactggc attgcaacca aagaaggagg aaaaactgct
     5641 gcaggtctcg gaggaattag tcatggaggc caaggctgct tttgaggatg ctcaggagga
     5701 agccagagcg gagaagctcc gagaagcact tccaccatta gtggcagaca aaggcatcga
     5761 ggcagccgca gaagttgtct gcgaagtgga ggggctccag gcggacatcg gagcagcatt
     5821 agttgaaacc ccgcgcggtc acgtaaggat aatacctcaa gcaaatgacc gtatgatcgg
     5881 acagtatatc gttgtctcgc caaactctgt gctgaagaat gccaaactcg caccagcgca
     5941 cccgctagca gatcaggtta agatcataac acactccgga agatcaggaa ggtacgcggt
     6001 cgaaccatac gacgctaaag tactgatgcc agcaggaggt gccgtaccat ggccagaatt
     6061 cctagcactg agtgagagcg ccacgttagt gtacaacgaa agagagtttg tgaaccgcaa
     6121 actataccac attgccatgc atggccccgc caagaataca gaagaggagc agtacaaggt
     6181 tacaaaggca gagcttgcag aaacagagta cgtgtttgac gtggacaaga agcgttgcgt
     6241 taagaaggaa gaagcctcag gtctggtcct ctcgggagaa ctgaccaacc ctccctatca
     6301 tgagctagct ctggagggac tgaagacccg acctgcggtc ccgtacaagg tcgaaacaat
     6361 aggagtgata ggcacaccgg ggtcgggcaa gtcagctatt atcaagtcaa ctgtcacggc
     6421 acgagatctt gttaccagcg gaaagaaaga aaattgtcgc gaaattgagg ccgacgtgct
     6481 aagactgagg ggtatgcaga ttacgtcgaa gacagtagat tcggttatgc tcaacggatg
     6541 ccacaaagcc gtagaagtgc tgtacgttga cgaagcgttc gcgtgccacg caggagcact
     6601 acttgccttg attgctatcg tcaggccccg caagaaggta gtactatgcg gagaccccat
     6661 gcaatgcgga ttcttcaaca tgatgcaact aaaggtacat ttcaatcacc ctgaaaaaga
     6721 catatgcacc aagacattct acaagtatat ctcccggcgt tgcacacagc cagttacagc
     6781 tattgtatcg acactgcatt acgatggaaa gatgaaaacc acgaacccgt gcaagaagaa
     6841 cattgaaatc gatattacag gggccacaaa gccgaagcca ggggatatca tcctgacatg
     6901 tttccgcggg tgggttaagc aattgcaaat cgactatccc ggacatgaag taatgacagc
     6961 cgcggcctca caagggctaa ccagaaaagg agtgtatgcc gtccggcaaa aagtcaatga
     7021 aaacccactg tacgcgatca catcagagca tgtgaacgtg ttgctcaccc gcactgagga
     7081 caggctagtg tggaaaacct tgcagggcga cccatggatt aagcagctca ctaacatacc
     7141 taaaggaaac tttcaggcta ctatagagga ctgggaagct gaacacaagg gaataattgc
     7201 tgcaataaac agccccactc cccgtgccaa tccgttcagc tgcaagacca acgtttgctg
     7261 ggcgaaagca ttggaaccga tactagccac ggccggtatc gtacttaccg gttgccagtg
     7321 gagcgaactg ttcccacagt ttgcggatga caaaccacat tcggccattt acgccttaga
     7381 cgtaatttgc attaagtttt tcggcatgga cttgacaagc ggactgtttt ctaaacagag
     7441 catcccacta acgtaccatc ccgccgattc agcgaggccg gtagctcatt gggacaacag
     7501 cccaggaacc cgcaagtatg ggtacgatca cgccattgcc gccgaactct cccgtagatt
     7561 tccggtgttc cagctagctg ggaagggcac acaacttgat ttgcagacgg ggagaaccag
     7621 agttatctct gcacagcata acctggtccc ggtgaaccgc aatcttcctc acgccttagt
     7681 ccccgagtac aaggagaagc aacccggccc ggtcgaaaaa ttcttgaacc agttcaaaca
     7741 ccactcagta cttgtggtat cagaggaaaa aattgaagct ccccgtaaga gaatcgaatg
     7801 gatcgccccg attggcatag ccggtgcaga taagaactac aacctggctt tcgggtttcc
     7861 gccgcaggca cggtacgacc tggtgttcat caacattgga actaaataca gaaaccacca
     7921 ctttcagcag tgcgaagacc atgcggcgac cttaaaaacc ctttcgcgtt cggccctgaa
     7981 ttgccttaac ccaggaggca ccctcgtggt gaagtcctat ggctacgccg accgcaacag
     8041 tgaggacgta gtcaccgctc ttgccagaaa gtttgtcagg gtgtctgcag cgagaccaga
     8101 ttgtgtctca agcaatacag aaatgtacct gattttccga caactagaca acagccgtac
     8161 acggcaattc accccgcacc atctgaattg cgtgatttcg tccgtgtatg agggtacaag
     8221 agatggagtt ggagccgcgc cgtcataccg caccaaaagg gagaatattg ctgactgtca
     8281 agaggaagca gttgtcaacg cagccaatcc gctgggtaga ccaggcgaag gagtctgccg
     8341 tgccatctat aaacgttggc cgaccagttt taccgattca gccacggaga caggcaccgc
     8401 aagaatgact gtgtgcctag gaaagaaagt gatccacgcg gtcggccctg atttccggaa
     8461 gcacccagaa gcagaagcct tgaaattgct acaaaacgcc taccatgcag tggcagactt
     8521 agtaaatgaa cataacatca agtctgtcgc cattccactg ctatctacag gcatttacgc
     8581 agccggaaaa gaccgccttg aagtatcact taactgcttg acaaccgcgc tagacagaac
     8641 tgacgcggac gtaaccatct attgcctgga taagaagtgg aaggaaagaa tcgacgcggc
     8701 actccaactt aaggagtctg taacagagct gaaggatgaa gatatggaga tcgacgatga
     8761 gttagtatgg attcatccag acagttgctt gaagggaaga aagggattca gtactacaaa
     8821 aggaaaattg tattcgtact tcgaaggcac caaattccat caagcagcaa aagacatggc
     8881 ggagataaag gtcctgttcc ctaatgacca ggaaagtaat gaacaactgt gtgcctacat
     8941 attgggtgag accatggaag caatccgcga aaagtgcccg gtcgaccata acccgtcgtc
     9001 tagcccgccc aaaacgttgc cgtgcctttg catgtatgcc atgacgccag aaagggtcca
     9061 cagacttaga agcaataacg tcaaagaagt tacagtatgc tcctccaccc cccttcctaa
     9121 gcacaaaatt aagaatgttc agaaggttca gtgcacgaaa gtagtcctgt ttaatccgca
     9181 cactcccgca ttcgttcccg cccgtaagta catagaagtg ccagaacagc ctaccgctcc
     9241 tcctgcacag gccgaggagg cccccgaagt tgtagcgaca ccgtcaccat ctacagctga
     9301 taacacctcg cttgatgtca cagacatctc actggatatg gatgacagta gcgaaggctc
     9361 acttttttcg agctttagcg gatcggacaa ctctattact agtatggaca gttggtcgtc
     9421 aggacctagt tcactagaga tagtagaccg aaggcaggtg gtggtggctg acgttcatgc
     9481 cgtccaagag cctgccccta ttccaccgcc aaggctaaag aagatggccc gcctggcagc
     9541 ggcaagaaaa gagcccactc caccggcaag caatagctct gagtccctcc acctctcttt
     9601 tggtggggta tccatgtccc tcggatcaat tttcgacgga gagacggccc gccaggcagc
     9661 ggtacaaccc ctggcaacag gccccacgga tgtgcctatg tctttcggat cgttttccga
     9721 cggagagatt gatgagctga gccgcagagt aactgagtcc gaacccgtcc tgtttggatc
     9781 atttgaaccg ggcgaagtga actcaattat atcgtcccga tcagccgtat cttttcctct
     9841 acgcaagcag agacgtagac gcaggagcag gaggactgaa tactgactaa ccggggtagg
     9901 tgggtacata ttttcgacgg acacaggccc tgggcacttg caaaagaagt ccgttctgca
     9961 gaaccagctt acagaaccga ccttggagcg caatgtcctg gaaagaattc atgccccggt
    10021 gctcgacacg tcgaaagagg aacaactcaa actcaggtac cagatgatgc ccaccgaagc
    10081 caacaaaagt aggtaccagt ctcgtaaagt agaaaatcag aaagccataa ccactgagcg
    10141 actactgtca ggactacgac tgtataactc tgccacagat cagccagaat gctataagat
    10201 cacctatccg aaaccattgt actccagtag cgtaccggcg aactactccg atccacagtt
    10261 cgctgtagct gtctgtaaca actatctgca tgagaactat ccgacagtag catcttatca
    10321 gattactgac gagtacgatg cttacttgga tatggtagac gggacagtcg cctgcctgga
    10381 tactgcaacc ttctgccccg ctaagcttag aagttacccg aaaaaacatg agtatagagc
    10441 cccgaatatc cgcagtgcgg ttccatcagc gatgcagaac acgctacaaa atgtgctcat
    10501 tgccgcaact aaaagaaatt gcaacgtcac gcagatgcgt gaactgccaa cactggactc
    10561 agcgacattc aatgtcgaat gctttcgaaa atatgcatgt aatgacgagt attgggagga
    10621 gttcgctcgg aagccaatta ggattaccac tgagtttgtc accgcatatg tagctagact
    10681 gaaaggccct aaggccgccg cactatttgc aaagacgtat aatttggtcc cattgcaaga
    10741 agtgcctatg gatagattcg tcatggacat gaaaagagac gtgaaagtta caccaggcac
    10801 gaaacacaca gaagaaagac cgaaagtaca agtgatacaa gccgcagaac ccctggcgac
    10861 tgcttactta tgcgggattc accgggaatt agtgcgtagg cttacggccg tcttgcttcc
    10921 aaacattcac acgctttttg acatgtcggc ggaggatttt gatgcaatca tagcagaaca
    10981 cttcaagcaa ggcgacccgg tactggagac ggatatcgca tcattcgaca aaagccaaga
    11041 cgacgctatg gcgttaaccg gtctgatgat cttggaggac ctgggtgtgg atcaaccact
    11101 actcgacttg atcgagtgcg cctttggaga aatatcatcc acccatctac ctacgggtac
    11161 tcgttttaaa ttcggggcga tgatgaaatc cggaatgttc ctcacacttt ttgtcaacac
    11221 agttttgaat gtcgttatcg ccagcagagt actagaagag cggcttaaaa cgtccagatg
    11281 tgcagcgttc attggcgacg acaacatcat acatggagta gtatctgaca aagaaatggc
    11341 tgagaggtgc gccacctggc tcaacatgga ggttaagatc atcgacgcag tcatcggtga
    11401 gagaccacct tacttctgcg gcggatttat cttgcaagat tcggttactt ccacagcgtg
    11461 ccgcgtggcg gatcccctga aaaggctgtt taagttgggt aaaccgctcc cagccgacga
    11521 cgagcaagac gaagacagaa gacgcgctct gctagatgaa acaaaggcgt ggtttagagt
    11581 aggtataaca ggcactttag cagtggccgt gacgacccgg tatgaggtag acaatattac
    11641 acctgtccta ctggcattga gaacttttgc ccagagcaaa agagcattcc aagccatcag
    11701 aggggaaata aagcatctct acggtggtcc taaatagtca gcatagtaca tttcatctga
    11761 ctaataccac aacaccacca cctctagcgc taccggactc agatctcgag ctcaagcttc
    11821 gaattcggct tggggatatc cacc
//
