LOCUS       sequence-292566-        1916 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression plasmid for SARS-CoV-2 ORF6 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1916)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046899
FEATURES             Location/Qualifiers
     source          1..1916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             272..292
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             293..310
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     rep_origin      431..1019
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     920..939
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1125..1784
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1191..1210)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1855..1889
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag actcttctat gtttcatctc gttgactttc aggttactat
      121 agcagagata ttactaatta ttatgaggac ttttaaagtt tccatttgga atcttgatta
      181 catcataaac ctcataatta aaaatttatc taagtcacta actgagaata aatattctca
      241 attagatgaa gagcaaccaa tggagattga tgagaacctg tactttcaaa gccaccatca
      301 tcaccatcat taaccggctt atcggtcagt ttcacctgat ttacgtaaaa acccgcttcg
      361 gcgggttttt gcttttggag gggcagaaag atgaatgact gtccacgacg ctatacccaa
      421 aagaaaaagc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
      481 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      541 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      601 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      661 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      721 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      781 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      841 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      901 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      961 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaat
     1021 tgatcgggca cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg
     1081 tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
     1141 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
     1201 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
     1261 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     1321 tgatgaatgc tcatccggaa tttcgtatgg caatgaaaga cggtgagctg gtgatatggg
     1381 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
     1441 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     1501 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgttt
     1561 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     1621 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     1681 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta
     1741 atgaattaca acagtactgc gatgagtggc agggcggggc gtaatttgat atcgagctcg
     1801 cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga
     1861 acgctcggtt gccgccgggc gttttttatt ggtcatcgat ctcgatcccg cgaaat
//
