LOCUS       sequence-292574-        1847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression plasmid for SARS-CoV-2 ORF10 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1847)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046746
FEATURES             Location/Qualifiers
     source          1..1847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      121..155
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     183..202
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        183..201
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    202..226
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             241..263
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             385..405
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             406..423
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     rep_origin      544..1132
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1033..1052
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(1238..1847,1..50)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1304..1323)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa tttgatatcg
       61 agctcgcttg gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg
      121 ttcagaacgc tcggttgccg ccgggcgttt tttattggtc atcgatctcg atcccgcgaa
      181 attaatacga ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat
      241 tttgtttaac tttaagaagg agactcttct atgggctata taaacgtttt cgcttttccg
      301 tttacgatat atagtctact cttgtgcaga atgaattctc gtaactacat agcacaagta
      361 gatgtagtta actttaatct cacagagaac ctgtactttc aaagccacca tcatcaccat
      421 cattaaccgg cttatcggtc agtttcacct gatttacgta aaaacccgct tcggcgggtt
      481 tttgcttttg gaggggcaga aagatgaatg actgtccacg acgctatacc caaaagaaaa
      541 agcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
      601 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
      661 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
      721 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
      781 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
      841 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
      901 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
      961 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     1021 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     1081 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aattgatcgg
     1141 gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt
     1201 tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
     1261 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
     1321 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
     1381 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
     1441 tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
     1501 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
     1561 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
     1621 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tttcagccaa
     1681 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
     1741 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
     1801 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaa
//
