LOCUS       sequence-292601-        4157 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module plasmid, individual component of Fungal Bioluminescent 
            Pathway, Luz, Luz promoter is circadian controlled.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-03-25"; volume: "9. pii: 52786. doi"; pages: " 
            10.7554/eLife.52786"
COMMENT     Sequence Label: P199
FEATURES             Location/Qualifiers
     source          1..4157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      176..764
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     665..684
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     918..935
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        3161..3265
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcctgatgtg cgggcttttt tttggtggtg gttaccagtg tcgcttagct accgtaggtc
       61 ctcttaggcg gtccgattag cgacatagtc atcaatgcct tgacggcaaa atcccttaac
      121 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
      181 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
      241 tggtttgttt gccggatcaa gagctactaa ctctttttcc gaaggtaact ggcttcagca
      301 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
      361 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
      421 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      481 agcggtcggg ctgaacgggg ggttcgtgct cacagcccag cttggagcga acgacctaca
      541 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      601 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      661 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      721 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
      781 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
      841 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
      901 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccacctgcaa
      961 cgtcccggcg cgccctatgg agccagttag tggattcatt tgtgaaacag tttggaaaat
     1021 gttgcggtta agaactgtat tgccaaaaat aaattaaatg tttttcatca ttactcattg
     1081 gtgactgaaa tgaattgagg tccctatact cttattgctt ctcagcatcg agttgtttgt
     1141 gcatcaccat tgtcatgact catgattttt agaatcattt atgcaattca tcatcttaat
     1201 cattatcatt ataggatgat tgtatcatat ataacagtcg aaagggaagg gccaaaaaaa
     1261 gtgaaataaa gttttgttct ttgtaatgga agaaaaagtt gggaggagaa tcatggtttc
     1321 actatggttc tttggaattt tgattttggt agttagtgcc caggttccgg gaaccaaatt
     1381 ctgatcatac tacaaaaaaa tatcttgcgg attattgtag ctcttgtctt gcataacatg
     1441 aagcttggaa ttaactaaag aaagtttctt agagattctt cttcttgttt gttctctaaa
     1501 gaatctaaca ctctttgtaa tgtttatcta aacttggaac taattagatg tgaaacacta
     1561 caattcatta aacatgagat catgcgtata ggaatttggt tcctgctgca ccgtggcaag
     1621 tcgtacacca tatacctttt tccctatttt attcatattt tactttatat tcactttaca
     1681 ataaatatct ttagatattt ctacaataag atccttatct ctctccatgg tttaattaaa
     1741 aataaatcca agaaaagtgc ataaaaagat atgtactttt tattcctcta cccaatcact
     1801 tcacagcttc actcacgcgc gtgttctttg atatcagata gatacgtcta cgtgagatac
     1861 acgctcttgt taatcaagtg gtgatgaaac agaacttact cgcctcggtg tccgattcta
     1921 ctcagaagat atttactata aatatctgat ttctctcgac tttgacgaaa atcaatgcgt
     1981 ataaatatct ctctgtctag cttattcgag agattaagca aactcagctc tcgtagcata
     2041 gctattacat gtggtgtcgt cctcgcctct gctattgctt ttcccatcat acgacgagat
     2101 tatcagacgt tcttagaggt tggtcctagc tatgcccctc aaaattttcg aggctacatt
     2161 atcgtttgcg tactcagctt gttccgacaa gagcaaaaag ggttggcaat ctacgacaga
     2221 ctcccagaga agagaagatg gctggccgat ctcccatttc gtgaaggtac tagacctagt
     2281 ataacatcac acataataca gagacagcga actcaacttg tagaccaaga gtttgccaca
     2341 cgagaactga tagacaaagt tattcccagg gtgcaagcac gacatacaga taaaacattt
     2401 ttgagcacga gcaagtttga atttcatgct aaagccattt tcctgttgcc aagtattcct
     2461 ataaacgacc ccttaaacat acctagtcat gatacggttc gtagaacgaa acgagagatt
     2521 gcacacatgc atgactacca cgactgtact cttcacttag ctcttgcagc tcaagatgga
     2581 aaagaagtcc tgaaaaaagg ctggggccaa cgtcatccgc ttgccgggcc gggtgtacct
     2641 gggccgccga cagaatggac tttcttatac gcaccccgta atgaggaaga agctagggta
     2701 gtagaaatga ttgtcgaggc aagtatcgga tacatgacta atgatccggc aggtaaaatc
     2761 gtcgagaatg ccaaatgagc ttgttcgagt attatggcat tgggaaaact gtttttcttg
     2821 taccatttgt tgtgcttgta atttactgtg ttttttattc ggttttcgct atcgaactgt
     2881 gaaatggaaa tggatggaga agagttaatg aatgatatgg tccttttgtt cattctcaaa
     2941 ttaatattat ttgttttttc tcttatttgt tgtgtgttga atttgaaatt ataagagata
     3001 tgcaaacatt ttgttttgag taaaaatgtg tcaaatcgtg gcctctaatg accgaagtta
     3061 atatgaggag taaaacactt gtagttgtcg ctgttagagc tcagtgacta gcaggtgatg
     3121 aaaagcctta gggtcaggtg gcacttttcg gggaaatgtg cgatgaaccc ctatttgttt
     3181 atttttctaa atacattcaa atatgtatcc gctaatgaga caataaccct gataaatgct
     3241 tcaattatat tgaaaaagga agagtatgag tatccaacat ttccgtgtcg cccttattcc
     3301 cttttttgcg gccttctgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     3361 agatgctgaa gatcagttgg gtgcccgagt gggttacatt gaactggatc tcaacagcgg
     3421 caaaatctta gagagttttc gccccgaaga acgcttccca atgatgagca ctttcaaagt
     3481 tctgctatgt ggcgcggtgt tatcccgtat tgatgctggg caagagcaac tcggtcgccg
     3541 catacactat tctcagaatg acttggttga gtattcacca gtcacagaaa agcatcttac
     3601 ggatggcatg acagtaagag aattatgtag tgctgccata accatgagtg ataacactgc
     3661 ggccaactta cttctgacaa cgattggagg accgaaggag ctaaccgctt ttttgcacaa
     3721 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     3781 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaactctcc gcaaactatt
     3841 aactggcgaa ctacttactc ttgcttcacg ccagcaactc attgactgga tggaggcgga
     3901 caaagttgca ggaccacttc tgcgctcggc acttccggct ggctggttta ttgctgataa
     3961 atctggagcc ggtgagcgtg gctctcgcgg tatcattgca gcactggggc cagatggtaa
     4021 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     4081 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagacgaagg
     4141 gaaataaata gtagccc
//
