LOCUS       sequence-292603-        9505 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Mtb MCM small and large subunit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9505)
  AUTHORS   Ruetz M, Campanello GC, Purchal M, Shen H, McDevitt L, Gouda H, 
            Wakabayashi S, Zhu J, Rubin EJ, Warncke K, Mootha VK, Koutmos M, 
            Banerjee R
  TITLE     Itaconyl-CoA forms a stable biradical in methylmalonyl-CoA mutase 
            and derails its activity and repair.
  JOURNAL   Science. 2019 Nov 1;366(6465):589-593. doi: 10.1126/science.aay0934.
  PUBMED    31672889
REFERENCE   2  (bases 1 to 9505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2019-11-1- 1"; volume: "366"; issue: 
            "6465"; pages: "589-593"
COMMENT     Sequence Label: pMtb MCM
FEATURES             Location/Qualifiers
     source          1..9505
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             100..117
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             130..150
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     promoter        2068..2086
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2087..2111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             4471..4515
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(4553..4571)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4567..4614
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4651..5106
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4738..4757)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4948..4969
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(5221..6012,6013..6081))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5844..5863
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6082..6174)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6255..6843
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6744..6763
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6997..7014
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7029..7168
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7094..7113)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7254..7276
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7270..7461)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8033..9115)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9077..9096
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9116..9193)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9399..9418
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atataccatg ggcagcagcc atcaccatca tcaccacagc
      121 caggatcctg agaacctgta cttccagagc gtgtccattg atgtacccga gcgtgccgac
      181 ctagaacagg ttcgcgggcg ctggcgcaac gcggttgccg gtgtgctgtc caagagcaac
      241 cgtaccgact cagcacaact cggcgatcac cccgagcggc tgctggatac ccagaccgct
      301 gacgggttcg ccatccgggc cctctacacc gcgttcgacg agctcccgga gccgccgttg
      361 ccgggccagt ggccctttgt gcgcggcgga gacccgctgc gcgacgtgca ttccggctgg
      421 aaggtcgccg aggcgtttcc cgccaacggt gcgacggccg acaccaacgc ggcggtgctg
      481 gccgcgctcg gcgagggggt cagcgcgctg ctgatccggg tgggggagtc gggtgtggcg
      541 cctgaccggc tcacggcgct gctgtccggg gtgtatctga acctggcgcc ggtcatcctc
      601 gacgccggcg ccgactaccg cccggcctgc gacgtcatgc tggcgctggt cgcccagctc
      661 gatcccggcc agcgcgacac cctgtcgatc gacctgggcg ccgacccgct gacggcgtcg
      721 ctgcgcgatc gtcccgcccc gccgatcgag gaggtcgtcg cggtcgcatc ccgggcggcc
      781 ggcgaacgtg ggcttcgtgc gatcaccgtc gacggaccgg ccttccacaa cctgggcgcg
      841 accgcggcca ccgaactcgc ggccaccgtc gcggccgcgg tggcctacct gcgggtgctc
      901 accgaatccg ggctcgtggt gagtgacgcg ctgcggcaga tcagcttccg gctcgccgcc
      961 gacgacgacc agttcatgac gctggccaag atgcgggctc tacgtcaact gtgggcgcgg
     1021 gtcgccgagg tcgtgggcga cccgggtggc ggcgcggccg tcgtgcacgc ggagacgtcg
     1081 ctaccgatga tgacccagcg tgatccgtgg gtgaacatgc tgcgctgcac gctggcggcc
     1141 ttcggcgccg gtgtcggtgg cgcggacacc gtgctggtgc acccgttcga cgtggcgatt
     1201 cccggcggct ttcccggcac ggcggccggc tttgcgcgcc ggatcgctcg caacacccaa
     1261 ctgctgcttt tagaagagtc gcatgtcggc agggtgctcg atcccgccgg cgggtcgtgg
     1321 ttcgtcgaag agctcaccga ccggctggct cggcgcgcct ggcagcgttt ccaggccatc
     1381 gaggcccgtg gcggcttcgt cgaggcccac gacttcctgg ccggccagat cgccgagtgc
     1441 gccgcccgcc gcgccgacga catcgcccat cggcgcctgg cgatcaccgg cgtcaacgaa
     1501 tacccgaacc tgggcgaacc cgcgctgccg cccggtgatc cgacatcgcc ggtgcgccgc
     1561 tacgctgccg gattcgaagc attgcgcgat cgatccgatc accacctagc ccgcactggc
     1621 gcacggccgc gggtgctgtt gctgccgttg ggtccgctgg ccgagcacaa catccggacg
     1681 accttcgcca ccaacctgct ggcgtccggc ggcatcgagg cgatcgaccc gggaacggtt
     1741 gatgcgggca ccgtcgggaa tgccgttgcc gatgccggtt cgcccagcgt tgccgtgatc
     1801 tgcggcaccg atgcgcgcta ccgggacgag gttgccgaca ttgtgcaagc ggcccgagcc
     1861 gccggtgttt cgagggtgta cctcgcgggt cccgagaagg cgttgggaga tgccgcacac
     1921 cggcccgacg agtttttgac cgcgaaaatc aatgtggtgc aagccttgtc gaatctgctg
     1981 acgcggttgg gggcctaagc ttgcggccgc ataatgctta agtcgaacag aaagtaatcg
     2041 tattgtacac ggccgcataa tcgaaattaa tacgactcac tataggggaa ttgtgagcgg
     2101 ataacaattc cccatcttag tatattagtt aagtataaga aggagatata catatgatga
     2161 caaccaagac acccgtgatc ggcagcttcg ccggcgttcc gctgcatagc gagcgtgccg
     2221 cgcaatcgcc cacagaggcc gcggtgcaca cgcatgtcgc cgccgccgcg gcggcgcacg
     2281 ggtacacgcc cgaacagttg gtgtggcaca cgccggaagg cattgacgtc acaccggtat
     2341 acatcgccgc cgaccgggcc gccgccgaag ccgagggcta cccgctgcac agcttcccgg
     2401 gcgagccccc ctttgtgcgc ggcccctatc cgacgatgta tgtgaaccag ccgtggacca
     2461 tccgccagta cgccgggttt tccaccgccg cggattccaa tgcgttttac cgacgcaacc
     2521 tggccgccgg ccagaagggg ctgtcggtgg ccttcgatct ggccacccac cgcggctacg
     2581 actccgacca tccccgcgtg cagggcgatg tcggaatggc cggtgtggca atcgattcca
     2641 ttctcgacat gcgacagctg ttcgacggca tcgacctgtc gaccgtgagc gtgtcgatga
     2701 cgatgaacgg tgcggtgctg ccgatcctgg cgctgtatgt ggttgccgcc gaggagcagg
     2761 gcgtggcgcc ggagcagctg gccggcacca tccagaacga catcctcaaa gagttcatgg
     2821 tccgcaacac ctacatctat ccgccgaagc cgtcgatgcg gatcatctcc gacatcttcg
     2881 cctacaccag cgccaagatg cccaagttca actccatctc catttccggc tatcacatcc
     2941 aagaagccgg tgccacggcg gatttggagc tggcctacac cctggccgac ggcgtcgact
     3001 acatcagggc gggcctgaac gccggcctgg acatcgacag cttcgcgccc cggctatcgt
     3061 tcttctgggg catcgggatg aatttcttta tggaggtcgc caaactgcgg gccggccggt
     3121 tgctgtggag tgagctggtc gcacagttcg cacccaagag cgccaaatcc ctttcgctgc
     3181 gtacacattc gcaaacatcg gggtggtcac tgaccgccca ggatgtgttc aacaacgtgg
     3241 cgcgcacatg catcgaggcg atggccgcca cccaggggca cacccagtcg ctgcacacca
     3301 acgccctgga cgaggcgctg gcgctgccca ccgatttttc ggcccgcatc gcgcgcaaca
     3361 cccagctggt gttgcagcag gagtcgggca ccacgcggcc gatcgacccg tgggggggct
     3421 cctactatgt ggagtggctg acccatcggc tcgcgcggcg agcccgggcg cacatcgccg
     3481 aggtcgctga acatggcggc atggcgcagg ccatcagcga cggcatcccc aagctgcgca
     3541 tcgaggaggc ggccgcgcgc acccaggccc gcatcgactc cggtcagcaa ccggtggtcg
     3601 gggtgaataa ataccaggtg cccgaggacc acgagatcga ggtgctcaag gtcgaaaaca
     3661 gccgggtgcg cgccgagcag ctggccaaac tgcagcggct gcgggcaggc cgggacgagc
     3721 cggcggtacg ggccgcgctg gccgagctga cccgcgccgc cgccgagcaa ggacgcgccg
     3781 gagcagacgg gctgggcaat aatctgctgg ccctggccat cgacgccgcc cgggcccagg
     3841 ccaccgtggg cgagatctcc gaagcgctgg agaaggtgta cggacggcac cgggccgaga
     3901 tccgtaccat ttccggggtc taccgcgacg aagttggaaa ggcccccaac atcgcagccg
     3961 caaccgagct agtggagaag ttcgccgagg ccgacggccg ccggcccagg attctgatcg
     4021 ccaagatggg ccaggacggc cacgaccgcg ggcagaaggt gatcgcgacc gcgttcgccg
     4081 acatcgggtt cgacgtcgac gtggggtcgc tgttttccac ccccgaggag gtggcgcgtc
     4141 aggccgccga caacgacgtg cacgtgatcg gggtgtcctc gctggccgcc ggccatctga
     4201 cgctggtgcc ggcgctgcgc gacgcgttgg cgcaggtggg caggcccgac atcatgatcg
     4261 tggtcggtgg tgtcatcccg ccgggcgact tcgacgagct gtacgccgcc ggggccaccg
     4321 ccattttccc gccggggacg gtgattgccg acgcggcgat tgacctgctg cacaggctgg
     4381 ccgagcggct ggggtacacg ctggattagg gatctcaatt ggatatcggc cggccacgcg
     4441 atcgctgacg tcggtaccct cgagtctggt aaagaaaccg ctgctgcgaa atttgaacgc
     4501 cagcacatgg actcgtctac tagcgcagct taattaacct aggctgctgc caccgctgag
     4561 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
     4621 ggaggaacta tatccggatt ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     4681 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     4741 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4801 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4861 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4921 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4981 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     5041 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     5101 caatttctgg cggcacgatg gcatgagatt atcaaaaagg atcttcacct agatcctttt
     5161 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     5221 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     5281 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     5341 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     5401 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     5461 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     5521 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     5581 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     5641 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     5701 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     5761 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     5821 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     5881 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     5941 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     6001 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     6061 acggaaatgt tgaatactca tactcttcct ttttcaatca tgattgaagc atttatcagg
     6121 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaataggtc
     6181 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6241 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6301 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6361 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     6421 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6481 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6541 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6601 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6661 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6721 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6781 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6841 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6901 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     6961 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     7021 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcaat
     7081 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
     7141 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
     7201 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     7261 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag
     7321 ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc
     7381 gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc
     7441 ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt
     7501 aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc
     7561 ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag
     7621 aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg
     7681 tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg
     7741 cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc
     7801 agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta
     7861 accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgct
     7921 agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg
     7981 tcgagatccc ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc
     8041 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     8101 gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca
     8161 acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg
     8221 tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc
     8281 tgtcttcggt atcgtcgtat cccactaccg agatgtccgc accaacgcgc agcccggact
     8341 cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg
     8401 gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt
     8461 cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag
     8521 ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt
     8581 gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa
     8641 tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg
     8701 cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga
     8761 cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta
     8821 ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa
     8881 tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt
     8941 tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt
     9001 ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg
     9061 tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca
     9121 ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc
     9181 attcgatggt gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag
     9241 cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag
     9301 atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg
     9361 ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg
     9421 ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc
     9481 gagatcgatc tcgatcccgc gaaat
//
