LOCUS       sequence-292626-        6242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Evaluating the impact of high sensor concentration on information 
            transmission of a genetic sensor..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6242)
  AUTHORS   Sarkar S, Tack D, Ross D
  TITLE     Sparse estimation of mutual information landscapes quantifies 
            information transmission through cellular biochemical reaction 
            networks.
  JOURNAL   Commun Biol. 2020 Apr 30;3(1):203. doi: 10.1038/s42003-020-0901-9.
  PUBMED    32355194
REFERENCE   2  (bases 1 to 6242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-04-30"; volume: "3(1):203. doi"; pages: " 
            10.1038/s42003-020-0901-9"
COMMENT     Sequence Label: pYFP-x-10
FEATURES             Location/Qualifiers
     source          1..6242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(75..80,81..96,97..103)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    109..128
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     misc_RNA        131..180
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     CDS             join(230..232,233..235,236..949)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKLHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(275..296)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(536..555)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     883..904
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2402..3484
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(2421..2440)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             3503..4126
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(3576..3595)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(4309..4328)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     rep_origin      complement(4693..5238)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(4773..4792)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5327..6142)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5327..6142)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6050..6069
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6134..6153
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ccaattattg aaggcctccc taacgggggg cctttttttg tttctggtct cccgcttaac
       61 gatcgttggc tgtgttgaca attaatcatc ggctcgtata atgtgtggaa ttgtgagcgc
      121 tcacaattag ctgtcaccgg atgtgctttc cggtctgatg agtccgtgag gacgaaacag
      181 cctctacaaa taattttgtt taatactaga gaaagagggg aaatactaga tggtgagcaa
      241 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
      301 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
      361 cctgaagttc atctgcacca caggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      421 cttcggctac ggcctgcaat gcttcgcccg ctaccccgac cacatgaagc tgcacgactt
      481 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
      541 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
      601 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
      661 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
      721 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
      781 gcagaacacc ccaatcggcg acggccccgt gctgctgccc gacaaccact accttagcta
      841 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
      901 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaat aataaccggg
      961 caggccatgt ctgcccgtat ttcgcgtaag gaaatccatt atgtactatt taaaaaacac
     1021 aaacttttgg atgttcggtt tattcttttt cttttacttt tttatcatgg gagcctactt
     1081 cccgtttttc ccgatttggc tacattaata atagcatatc agcaaaagtg atacgggtat
     1141 tatttttgcc gctatttctc tgttctcgct attattccaa ccgctgtttg gtctgctttc
     1201 tgacaaactc gggctgcgca aatacctgct gtggattatt accggcatgt tagtgatgtt
     1261 tgcgccgttc tttattttta tcttcgggcc actgttacaa tacaacattt tagtaggatc
     1321 gattgttggt ggtatttatc taggcttttg ttttaacgcc ggtgcgccag cagtagaggc
     1381 atttattgag aaagtcagcc gtcgcagtaa tttcgaattt ggtcgcgcgc ggatgtttgg
     1441 ctgtgttggc tgggcgctgt gtgcctcgat tgtcggcatc atgttcacca tcaataatca
     1501 gtttgttttc tggctgggct ctggctgtgc actcatcctc gccgttttac tctttttcgc
     1561 caaaacggat gcgccctctt ctgccacggt tgccaatgcg gtaggtgcca accattcggc
     1621 atttagcctt aagctggcac tggaactgtt cagacagcca aaactgtggt ttttgtcact
     1681 gtatgttatt ggcgtttcct gcacctacga tgtttttgac caacagtttg ctaatttctt
     1741 tacttcgttc tttgctaccg gtgaacaggg tacgcgggta tttggctacg taacgacaat
     1801 gggcgaatta cttaacgcct cgattatgtt ctttgcgcca ctgatcatta atcgcatcgg
     1861 tgggaaaaac gccctgctgc tggctggcac tattatgtct gtacgtatta ttggctcatc
     1921 gttcgccacc tcagcgctgg aagtggttat tctgaaaacg ctgcatatgt ttgaagtacc
     1981 gttcctgctg gtgggctgct ttaaatatat taccagccag tttgaagtgc gtttttcagc
     2041 gacgatttat ctggtctgtt tctgcttctt taagcaactg gcgatgattt ttatgtctgt
     2101 actggcgggc aatatgtatg aaagcatcgg tttccagggc gcttatctgg tgctgggtct
     2161 ggtggcgctg ggcttcacct taatttccgt gttcacgctt agcggccccg gcccgctttc
     2221 cctgctgcgt cgtcaggtga atgaagtcgc ttaactcggt accaaattcc agaaaagagg
     2281 cctcccgaaa ggggggcctt ttttcgtttt ggtccaatgg cggcgcgcca tcgaatggcg
     2341 caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattaa ggagagtgaa
     2401 tatgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt
     2461 ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc
     2521 ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca
     2581 gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt
     2641 cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga
     2701 acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag
     2761 tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg
     2821 cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat
     2881 tttctcccat gaggacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca
     2941 gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg
     3001 ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg
     3061 gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac
     3121 tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc
     3181 cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg aagatagctc
     3241 atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag
     3301 cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc
     3361 agtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg
     3421 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     3481 gtgataatcc aggaggaaaa aaatgtccag attagataaa agtaaagtga ttaacagcgc
     3541 attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac tcgcccagaa
     3601 gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg ctttgctcga
     3661 cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt tagaagggga
     3721 aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt tactaagtca
     3781 tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt atgaaactct
     3841 cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg cattatatgc
     3901 actcagcgct gtggggcatt ttactttagg ttgcgtattg gaagatcaag agcatcaagt
     3961 cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat tacgacaagc
     4021 tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc ttgaattgat
     4081 catatgcgga ttagaaaaac aacttaaatg tgaaagtggg tcctaataat tggtaacgaa
     4141 tcagacaatt gacggctcga gggagtagca tagggtttgc agaatccctg cttcgtccat
     4201 ttgacaggca cattatgcat cgatgataag ctgtcaaaca tgagcagatc ctctacgccg
     4261 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
     4321 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcaaa tattttatct
     4381 gaggtgcttc ctcgctcact gactcgctgc acgaggcaga cctcagcgct agcggagtgt
     4441 atactggctt actatgttgg cactgatgag ggtgtcagtg aagtgcttca tgtggcagga
     4501 gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata caggatatat tccgcttcct
     4561 cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg gcttacgaac
     4621 ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc
     4681 ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc
     4741 aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg
     4801 ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg
     4861 gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc
     4921 tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc
     4981 gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt
     5041 gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt
     5101 tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga
     5161 accttcgaaa aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc
     5221 agaccaaaac gatctcaaga agatcatctt attaaggggt ctgacgctca gtggaacgaa
     5281 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccttag aaaaactcat
     5341 cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa
     5401 aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat
     5461 cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct
     5521 cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga
     5581 atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt
     5641 catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac
     5701 gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca
     5761 ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct
     5821 ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga
     5881 taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct
     5941 catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat
     6001 cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc
     6061 atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctg gagcaagacg
     6121 tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt
     6181 ttattgttca tgatgatata tttttatctt gtgcaatgta catcagagat tttgagacac
     6241 aa
//
