LOCUS       sequence-292634-       10902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (G112P 
            mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10902)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 10902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-myc-HLA-A2-VN (G112P)
FEATURES             Location/Qualifiers
     source          1..10902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(710..728)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        796..900
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(901..969,970..1761)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1119..1138)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1932..2520
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2421..2440
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2951..3096
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3139..4176
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4174..4193)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4218..4266
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4920..5299
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5300..5503
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5454..5474
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5500..5524
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5606..5635
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(6683..6685,6686..7144)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYLTA"
     primer_bind     complement(6725..6746)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6986..7005)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    7167..7301
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7191..7210)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7245..7264
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7444..7466)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8026..9815
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 cctgctcctg ttccaccgtg ggtccctttg cagccaatgc aacttggacg tttttggggt
       61 ctccggacac catctctatg tcttggccct gatcctgagc cgcccggggc tcctggtctt
      121 ccgcctcctc gtcctcgtcc tcttccccgt cctcgtccat ggttatcacc ccctcttctt
      181 tgaggtccac tgccgccgga gccttctggt ccagatgtgt ctcccttctc tcctaggcca
      241 tttccaggtc ctgtacctgg cccctcgtca gacatgattc acactaaaag agatcaatag
      301 acatctttat tagacgacgc tcagtgaata cagggagtgc agactcctgc cccctccaac
      361 agccccccca ccctcatccc cttcatggtc gctgtcagac agatccaggt ctgaaaattc
      421 cccatcctcc gaaccatcct cgtcctcatc accaattact cgcagcccgg aaaactcccg
      481 ctgaacatcc tcaagatttg cgtcctgagc ctcaagccag gcctcaaatt cctcgtcccc
      541 ctttttgctg gacggtaggg atggggattc tcgggacccc tcctcttcct cttcaaggtc
      601 accagacaga gatgctactg gggcaacgga agaaaagctg ggtgcggcct gtgaggatca
      661 gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata
      721 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
      781 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
      841 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
      901 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
      961 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     1021 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     1081 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     1141 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     1201 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     1261 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     1321 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     1381 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     1441 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     1501 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     1561 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     1621 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     1681 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     1741 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     1801 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     1861 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1921 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1981 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2041 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     2101 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2161 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2221 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2281 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2341 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2401 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2461 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2521 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttg aagctgtccc
     2581 tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg
     2641 ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc
     2701 aagacgtagc ccagcgcgtc ggccccgaga tgcgccgcgt gcggctgctg gagatggcgg
     2761 acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt
     2821 gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccct gcttcatccc
     2881 cgtggcccgt tgctcgcgtt tgctggcggt gtccccggaa gaaatatatt tgcatgtctt
     2941 tagttctatg atgacacaaa ccccgcccag cgtcttgtca ttggcgaatt cgaacacgca
     3001 gatgcagtcg gggcggcgcg gtccgaggtc cacttcgcat attaaggtga cgcgtgtggc
     3061 ctcgaacacc gagcgaccct gcagcgaccc gcttaacagc gtcaacagcg tgccgcagat
     3121 cccggggggc aatgagatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt
     3181 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
     3241 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
     3301 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt
     3361 ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt
     3421 gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg
     3481 gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc
     3541 ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt
     3601 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc
     3661 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc
     3721 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc
     3781 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc
     3841 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg
     3901 gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc
     3961 tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac
     4021 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg
     4081 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
     4141 actcgtccgg atcgggagat gggggaggct aactgaaaca cggaaggaga caataccgga
     4201 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt
     4261 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
     4321 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
     4381 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gccactggcc
     4441 ccgtgggtta gggacggggt cccccatggg gaatggttta tggttcgtgg gggttattat
     4501 tttgggcgtt gcgtggggtc aggtccacga ctggactgag cagacagacc catggttttt
     4561 ggatggcctg ggcatggacc gcatgtactg gcgcgacacg aacaccgggc gtctgtggct
     4621 gccaaacacc cccgaccccc aaaaaccacc gcgcggattt ctggcgtgcc aagctagtcg
     4681 accaattctc atgtttgaca gcttatcatc gcagatccgg gcaacgttgt tgccattgct
     4741 gcaggcgcag aactggtagg tatggaagat ctatacattg aatcaatatt ggcaattagc
     4801 catattagtc attggttata tagcataaat caatattggc tattggccat tgcatacgtt
     4861 gtatctatat cataatatgt acatttatat tggctcatgt ccaatatgac cgccatgttg
     4921 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     4981 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     5041 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     5101 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     5161 agtgtatcat atgccaagtc cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     5221 gcattatgcc cagtacatga ccttacggga ctttcctact tggcagtaca tctacgtatt
     5281 agtcatcgct attaccatgg tgatgcggtt ttggcagtac accaatgggc gtggatagcg
     5341 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     5401 gcaccaaaat caacgggact ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat
     5461 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     5521 gatctctaga agctgggtac cagctgctag cgccaccatg aagaccatca tcgccctgag
     5581 ctacatcttc tgcctggtgt tcgccgagca gaaattgatc agcgaggagg acctgggcag
     5641 ccctggaggc agttcaggag gcggatctca ctccatgagg tatttcttca catccgtgtc
     5701 ccggcccggc cgcggggagc cccgcttcat cgcagtgggc tacgtggacg acacgcagtt
     5761 cgtgcggttc gacagcgacg ccgcgagcca gaggatggag ccgcgggcgc cgtggataga
     5821 gcaggagggt ccggagtatt gggacgggga gacacggaaa gtgaaggccc actcacagac
     5881 tcaccgagtg gacctgggga ccctgcgcgg ctactacaac cagagcgagg ccggttctca
     5941 caccgtccag aggatgtatg gctgcgacgt ggggtcggac tggcgcttcc tccgccctta
     6001 ccaccagtac gcctacgacg gcaaggatta catcgccctg aaagaggacc tgcgctcttg
     6061 gaccgcggcg gacatggcag ctcagaccac caagcacaag tgggaggcgg cccatgtggc
     6121 ggagcagttg agagcctacc tggagggcac gtgcgtggag tggctccgca gatacctgga
     6181 gaacgggaag gagacgctgc agcgtacgga cgcccccaaa acgcatatga ctcaccacgc
     6241 tgtctctgac catgaagcca ccctgaggtg ctgggccctg agcttctacc ctgcggagat
     6301 cacactgacc tggcagcggg atggggagga ccagacccag gacacggagc tcgtggagac
     6361 caggcctgca ggggatggaa ccttccagaa gtgggcggct gtggtggtgc cttctggaca
     6421 ggagcagaga tacacctgcc atgtgcagca tgagggtttg cccaagcccc tcaccctgag
     6481 atgggagccg tcttcccagc ccaccatccc catcgtgggc atcattgctg gcctggttct
     6541 ctttggagct gtgatcactg gagctgtggt cgctgctgtg atgtggagga ggaagagctc
     6601 agatagaaaa ggagggagct actctcaggc tgcaagcagt gacagtgccc agggctctga
     6661 tgtgtctctc acagcttgta aagtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     6721 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     6781 cgagggcgat gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct
     6841 gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg
     6901 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     6961 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     7021 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     7081 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatctgac
     7141 cgcctaactc gaggccggca aggccggatc cagacatgat aagatacatt gatgagtttg
     7201 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     7261 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     7321 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
     7381 acaaatgtgg tatggctgat tatgatccgg ctgcctcgcg cgtttcggtg atgacggtga
     7441 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     7501 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     7561 gaggtcgact ctagaggatc gatccccgcc ccggacgaac taaacctgac tacgacatct
     7621 ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta caacttgcca
     7681 actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg ttctctgact
     7741 gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg ctttcatcct
     7801 ggagcagact ttgcagtctg tggactgcaa cacaacattg cctttatgtg taactcttgg
     7861 ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag gaagactacg
     7921 ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc ggaccctcaa
     7981 gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg gtagcatatg
     8041 cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca tatgttaccc
     8101 aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga acagcgatat
     8161 ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc acgagggtag
     8221 tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag tgaactctcc
     8281 tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac tgctgagttg
     8341 tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg cccccagaat
     8401 aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat aaccctcaca
     8461 aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc tttaacaata
     8521 gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca cgaatttatg
     8581 gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag atgtgtccca
     8641 ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa ggggaaagag
     8701 agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga aaattaaacg
     8761 gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt ttttgaaatt
     8821 gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga ctgtaaaata
     8881 agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa ccacttgccc
     8941 acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg ggccaagata
     9001 ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg agcgccaagc
     9061 acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc taatgttgcc
     9121 atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg
     9181 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     9241 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg
     9301 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg
     9361 gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat ttatatctgg
     9421 gtagcatata ctacccaaat atctggatag catatgctat cctaatctat atctgggtag
     9481 catatgctat cctaatctat atctgggtag cataggctat cctaatctat atctgggtag
     9541 catatgctat cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag
     9601 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     9661 tatatgctat cctaatctgt atccgggtag catatgctat cctcatgcat atacagtcag
     9721 catatgatac ccagtagtag agtgggagtg ctatcctttg catatgccgc cacctcccaa
     9781 gggggcgtga attttcgctg cttgtccttt tcctgctggt tgctcccatt cttaggtgaa
     9841 tttaaggagg ccaggctaaa gccgtcgcat gtctgattgc tcaccaggta aatgtcgcta
     9901 atgttttcca acgcgagaag gtgttgagcg cggagctgag tgacgtgaca acatgggtat
     9961 gcccaattgc cccatgttgg gaggacgaaa atggtgacaa gacagatggc cagaaataca
    10021 ccaacagcac gcatgatgtc tactggggat ttattcttta gtgcggggga atacacggct
    10081 tttaatacga ttgagggcgt ctcctaacaa gttacatcac tcctgccctt cctcaccctc
    10141 atctccatca cctccttcat ctccgtcatc tccgtcatca ccctccgcgg cagccccttc
    10201 caccataggt ggaaaccagg gaggcaaatc tactccatcg tcaaagctgc acacagtcac
    10261 cctgatattg caggtaggag cgggctttgt cataacaagg tccttaatcg catccttcaa
    10321 aacctcagca aatatatgag tttgtaaaaa gaccatgaaa taacagacaa tggactccct
    10381 tagcgggcca ggttgtgggc cgggtccagg ggccattcca aaggggagac gactcaatgg
    10441 tgtaagacga cattgtggaa tagcaagggc agttcctcgc cttaggttgt aaagggaggt
    10501 cttactacct ccatatacga acacaccggc gacccaagtt ccttcgtcgg tagtcctttc
    10561 tacgtgactc ctagccagga gagctcttaa accttctgca atgttctcaa atttcgggtt
    10621 ggaacctcct tgaccacgat gctttccaaa ccaccctcct tttttgcgcc tgcctccatc
    10681 accctgaccc cggggtccag tgcttgggcc ttctcctggg tcatctgcgg ggccctgctc
    10741 tatcgctccc gggggcacgt caggctcacc atctgggcca ccttcttggt ggtattcaaa
    10801 ataatcggct tcccctacag ggtggaaaaa tggccttcta cctggagggg gcctgcgcgg
    10861 tggagacccg gatgatgatg actgactact gggactcctg gg
//
