LOCUS       sequence-292637-        3958 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module plasmid, individual component of Fungal Bioluminescent 
            Pathway, H3H.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-03-25"; volume: "9. pii: 52786. doi"; pages: " 
            10.7554/eLife.52786"
COMMENT     Sequence Label: P368
FEATURES             Location/Qualifiers
     source          1..3958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      170..758
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     659..678
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     912..929
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        979..1359
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     terminator      2631..2883
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     promoter        2956..3060
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgtgcgggct tttttttggt ggtggttacc agtgtcgctt agctaccgta ggtcctctta
       61 ggcggtccga ttagcgacat agtcatcaat gccttgacgg caaaatccct taacgtgagt
      121 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
      181 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
      241 gtttgccgga tcaagagcta ctaactcttt ttccgaaggt aactggcttc agcagagcgc
      301 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
      361 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
      421 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
      481 cgggctgaac ggggggttcg tgctcacagc ccagcttgga gcgaacgacc tacaccgaac
      541 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
      601 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
      661 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
      721 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
      781 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
      841 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
      901 cgaccgagcg cagcgagtca gtgagcgagg aagcgggaga gcgcccacct gccaggggac
      961 ggcgcgccct atggagattt ttcaaatcag tgcgcaagac gtgacgtaag tatccgagtc
     1021 agtttttatt tttctactaa tttggtcgtt tatttcggcg tgtaggacat ggcaaccggg
     1081 cctgaatttc gcgggtattc tgtttctatt ccaacttttt cttgatccgc agccattaac
     1141 gacttttgaa tagatacgct gacacgccaa gcctcgctag tcaaaagtgt accaaacaac
     1201 gctttacagc aagaacggaa tgcgcgtgac gctcgcggtg acgccatttc gccttttcag
     1261 aaatggataa atagccttgc ttcctattat atcttcccaa attaccaata cattacacta
     1321 gcatctgaat ttcataacca atctcgatac accaaatcga atggcatcct ttgaaaattc
     1381 actcagtgtc ttaatagttg gggctggctt aggtggtttg gccgctgcta tagcactgcg
     1441 aaggcaagga catgtagtca agatatacga tagttcttca ttcaaggccg aattgggagc
     1501 aggattagct gtaccaccca atactctccg ttccctgcaa caactgggct gtaatacaga
     1561 aaacctgaac ggagtagaca acctctgctt tacggccatg ggatacgacg ggagtgtagg
     1621 tatgatgaat aatatgaccg actaccgtga ggcatacggc acttcctgga taatggtaca
     1681 tcgagtggat ctccacaacg agctgatgcg agtcgccttg gatccgggcg ggttagggcc
     1741 tcctgccact cttcacctta accatcgtgt tacgttctgc gatgtggatg cctgtacagt
     1801 cacgtttacg aacgggacga cacagtctgc tgacttgatt gtcggagctg atggtattag
     1861 gtctacgatt cgaagattcg ttctggagga ggacgtgact gttccagcta gcggaattgt
     1921 cggctttcga tggttggtcc aggccgatgc tctcgaccca tatcccgaac tggattggat
     1981 tgtcaaaaaa ccacccttgg gagccaggtt gatttctact ccacagaacc cgcagagtgg
     2041 cgtaggactg gccgatagac gaacgatcat tatttacgca tgcagaggtg ggaccatggt
     2101 taatgtatta gccgtacacg acgacgaaag agatcaaaac actgctgatt ggtccgttcc
     2161 cgcatcaaag gatgatttat tcagagtgtt tcacgattac catccaagat tccgacgact
     2221 cttagagtta gcacaggaca ttaatttatg gcagatgagg gtggtcccgg tcttgaaaaa
     2281 atgggtgaac aagagggtat gcttgctggg tgatgccgcc catgcttcac tgcccacact
     2341 tggacaaggc ttcgggatgg ggttggagga tgcagtcgct ttgggcacgt tgttgcctaa
     2401 gggaacgacc gcatcacaga tcgagacaag gctggccgtt tacgaacagc tgcgaaagga
     2461 tcgagctgag tttgtcgccg cagagagcta tgaggagcaa tatgtcccag agatgagagg
     2521 gctgtacctt aggtccaaag aattgagaga cagagtgatg ggttatgata ttaaggtaga
     2581 gtcagagaag gttttggaaa cactgttgcg atcatccaac tcagcttgag cttggactcc
     2641 catgttggca aaggcaacca aacaaacaat gaatgatccg ctcctgcata tggggcggtt
     2701 tgagtatttc aactgccatt tgggctgaat tgtagacatg ctcctgtcag aaattccgtg
     2761 atcttactca atattcagta atctcggcca atatcctaaa tgtgcgtggc tttatctgtc
     2821 tttgtattgt ttcatcaatt catgtaacgt ttgcttttct tatgaatttt caaataaatt
     2881 atccgctgtt agagctcccg gtgacgcagg tgatgaaaag ccttagggtc aggtggcact
     2941 tttcggggaa atgtgcgatg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3001 tatccgctaa tgagacaata accctgataa atgcttcaat tatattgaaa aaggaagagt
     3061 atgagtatcc aacatttccg tgtcgccctt attccctttt ttgcggcctt ctgccttcct
     3121 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgcc
     3181 cgagtgggtt acattgaact ggatctcaac agcggcaaaa tcttagagag ttttcgcccc
     3241 gaagaacgct tcccaatgat gagcactttc aaagttctgc tatgtggcgc ggtgttatcc
     3301 cgtattgatg ctgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     3361 gttgagtatt caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     3421 tgtagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatt
     3481 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     3541 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     3601 cctgtagcaa tggcaacaac tctccgcaaa ctattaactg gcgaactact tactcttgct
     3661 tcacgccagc aactcattga ctggatggag gcggacaaag ttgcaggacc acttctgcgc
     3721 tcggcacttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtggctct
     3781 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     3841 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     3901 tcactgatta agcattggta actgtcagac gaagggaaat aaatagtagc ccgcctga
//
