LOCUS       sequence-292640-        5053 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module plasmid, individual component of Fungal Bioluminescent 
            Pathway, Luz, promoter is ABA responsive.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-03-25"; volume: "9. pii: 52786. doi"; pages: " 
            10.7554/eLife.52786"
COMMENT     Sequence Label: P442
FEATURES             Location/Qualifiers
     source          1..5053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      171..759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     660..679
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     913..930
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      3269..3976
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        4052..4156
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atgtgcgggc ttttttttgg tggtggttac cagtgtcgct tagctaccgt aggtcctctt
       61 aggcggtccg attagcgaca tagtcatcaa tgccttgacg gcaaaatccc ttaacgtgag
      121 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
      181 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
      241 tgtttgccgg atcaagagct actaactctt tttccgaagg taactggctt cagcagagcg
      301 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
      361 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
      421 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
      481 tcgggctgaa cggggggttc gtgctcacag cccagcttgg agcgaacgac ctacaccgaa
      541 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
      601 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
      661 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
      721 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
      781 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
      841 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
      901 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccacc tgcaacgtcc
      961 cggcgcgccc tatggagaag ctttccagac acatcttggg attagatcat agtccatact
     1021 aatgaagaca aaagtgttaa ccaaatatag ataatgagag aatgtggggg gtgaagttga
     1081 accctgagtg ctgcaatcct atctgcgtgc aacttttcta gctctggatc ctacagaaca
     1141 agagcaagtc agtctcccat agagacagaa gagaaacata acatttcatt gtacagatta
     1201 taaaagaaca attcaaagag agcccccatt gaacttacat ccatcaattc gtcaagatca
     1261 acttcctcgt tgacaggtct tgatccttgt gccttttcat ttgcaagaac ttcctgcaca
     1321 acaaaaatac ggaaatgttt ttgatccaag aacatcaaac tctaattgca attgtaacca
     1381 acaaaaaaac acttaaaatt cgctatacac tatcaaattt cctgacacgt agacctcatg
     1441 tcagcacaaa tagaatctag cctatctctg ttaattgggt taccaaacaa catgagattg
     1501 attatgtgga aaaccaagca cattatttac cctttgaaaa tacgcgaaat caagatccaa
     1561 gaagaattta tggagaacag cttcgaagta cgaaataaac aatgaagatt aaattacctt
     1621 tttataatct ctagcagctg ccgccaatac attcccgaat gccagattcg agagggtcga
     1681 cttcaccgta tccggatcca tctctttacc aaccaactaa tccaactcag aaaattttaa
     1741 aatctcaatc aaaaatccct ctaagatagc cagagaagag attgtaaaca aggatttgaa
     1801 atctggtgca gagaggagaa actccccgac aatgaacacc aacgatctaa acgcggcgtt
     1861 tggtaaaagt tgagtaaatt ttgttagggc ttagttttag tccatgggct aattagtaag
     1921 tgatttacgg cccacacatg agcccaaatg tttcagaccc agccaagttt cttcaaattc
     1981 acccaatcaa cgacgatgta cgtgtgtatg aaaatcatta acacgacgca tcgctttcga
     2041 ggaggagcat tacgtgtcct gttagctacg ataatgttag taccgccaca aagaaaagga
     2101 tagatatttt gctttccagc accctgtcat gggattgata tgaacacgta cttggtatcg
     2161 acatgaaagc tcaaaaataa attcaatccg attcctttag tgatatcaga agttcatttt
     2221 aaatacgaac acgtatggcg aaacaccacg ccgacatttt ctgctgctgc cacgcgtcac
     2281 tttccaaata ttgattcatt aaactaatag ttgatccata tccgaaaccg gactataaaa
     2341 ctatcttcaa tgcgttaacg aatcttcatc gatcaaactc atcaaagtct aatatcacaa
     2401 agaaagagtt tttttaacta gcttagctca aagtgtttgc ttaagacaag aagaacaatg
     2461 cgtataaata tctctctgtc tagcttattc gagagattaa gcaaactcag ctctcgtagc
     2521 atagctatta catgtggtgt cgtcctcgcc tctgctattg cttttcccat catacgacga
     2581 gattatcaga cgttcttaga ggttggtcct agctatgccc ctcaaaattt tcgaggctac
     2641 attatcgttt gcgtactcag cttgttccga caagagcaaa aagggttggc aatctacgac
     2701 agactcccag agaagagaag atggctggcc gatctcccat ttcgtgaagg tactagacct
     2761 agtataacat cacacataat acagagacag cgaactcaac ttgtagacca agagtttgcc
     2821 acacgagaac tgatagacaa agttattccc agggtgcaag cacgacatac agataaaaca
     2881 tttttgagca cgagcaagtt tgaatttcat gctaaagcca ttttcctgtt gccaagtatt
     2941 cctataaacg accccttaaa catacctagt catgatacgg ttcgtagaac gaaacgagag
     3001 attgcacaca tgcatgacta ccacgactgt actcttcact tagctcttgc agctcaagat
     3061 ggaaaagaag tcctgaaaaa aggctggggc caacgtcatc cgcttgccgg gccgggtgta
     3121 cctgggccgc cgacagaatg gactttctta tacgcacccc gtaatgagga agaagctagg
     3181 gtagtagaaa tgattgtcga ggcaagtatc ggatacatga ctaatgatcc ggcaggtaaa
     3241 atcgtcgaga atgccaaatg agcttgtcct gctttaatga gatatgcgag aagcctatga
     3301 tcgcatgata tttgctttca attctgttgt gcacgttgta aaaaacctga gcatgtgtag
     3361 ctcagatcct taccgccggt ttcggttcat tctaatgaat atatcacccg ttactatcgt
     3421 atttttatga ataatattct ccgttcaatt tactgattgt accctactac ttatatgtac
     3481 aatattaaaa tgaaaacaat atattgtgct gaataggttt atagcgacat ctatgataga
     3541 gcgccacaat aacaaacaat tgcgttttat tattacaaat ccaattttaa aaaaagcggc
     3601 agaaccggtc aaacctaaaa gactgattac ataaatctta ttcaaatttc aaaagtgccc
     3661 caggggctag tatctacgac acaccgagcg gcgaactaat aacgctcact gaagggaact
     3721 ccggttcccc gccggcgcgc atgggtgaga ttccttgaag ttgagtattg gccgtccgct
     3781 ctaccgaaag ttacgggcac cattcaaccc ggtccagcac ggcggccggg taaccgactt
     3841 gctgccccga gaattatgca gcattttttt ggtgtatgtg ggccccaaat gaagtgcagg
     3901 tcaaaccttg acagtgacga caaatcgttg ggcgggtcca gggcgaattt tgcgacaaca
     3961 tgtcgaggct cagcaggacc gctgttagag ctcagtgact agcaggtgat gaaaagcctt
     4021 agggtcaggt ggcacttttc ggggaaatgt gcgatgaacc cctatttgtt tatttttcta
     4081 aatacattca aatatgtatc cgctaatgag acaataaccc tgataaatgc ttcaattata
     4141 ttgaaaaagg aagagtatga gtatccaaca tttccgtgtc gcccttattc ccttttttgc
     4201 ggccttctgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     4261 agatcagttg ggtgcccgag tgggttacat tgaactggat ctcaacagcg gcaaaatctt
     4321 agagagtttt cgccccgaag aacgcttccc aatgatgagc actttcaaag ttctgctatg
     4381 tggcgcggtg ttatcccgta ttgatgctgg gcaagagcaa ctcggtcgcc gcatacacta
     4441 ttctcagaat gacttggttg agtattcacc agtcacagaa aagcatctta cggatggcat
     4501 gacagtaaga gaattatgta gtgctgccat aaccatgagt gataacactg cggccaactt
     4561 acttctgaca acgattggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     4621 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     4681 gcgtgacacc acgatgcctg tagcaatggc aacaactctc cgcaaactat taactggcga
     4741 actacttact cttgcttcac gccagcaact cattgactgg atggaggcgg acaaagttgc
     4801 aggaccactt ctgcgctcgg cacttccggc tggctggttt attgctgata aatctggagc
     4861 cggtgagcgt ggctctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     4921 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     4981 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagacgaag ggaaataaat
     5041 agtagcccgc ctg
//
