LOCUS       sequence-292649-        7347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  individual component of Fungal Bioluminescent Pathway, MoClo level 0
            plasmid, NnHisps.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7347)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 7347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-03-25"; volume: "9. pii: 52786. doi"; pages: " 
            10.7554/eLife.52786"
COMMENT     Sequence Label: pCO600
FEATURES             Location/Qualifiers
     source          1..7347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(5145..5163)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5231..5335
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5557..6348
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6519..7107
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7008..7027
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7261..7278
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcaaat gaatagtagt aaaaaccctc catccactct gttagatgtt tttctggata
       61 ccgctaggaa ccttgataca gcatccagaa atgtcctgga gtgcggagaa cacaggtgga
      121 gttacagaga gttggacaca gtgtcatccg cattggccca acaccttcga tacactgtgg
      181 gtcttagccc cactgtagcc gtgattagcg agaatcaccc ctacatactt gcattaatgc
      241 tcgccgtatg gaaactcggc ggaacgttcg caccgataga tgttcattcc ccagccgaac
      301 ttgttgctgg aatgttgaat attgtaagcc cgtcatgtct tgtcattcct agctctgatg
      361 tgacaaatca gacattggca tgcgatctta atataccggt tgttgcattc cacccacatc
      421 aaagtacaat tcccgaactc aacaagaagt atctcacaga ttctcagatc agtcctgacc
      481 tgcccttccc cgaccctaat cgacctgctt tgtacttatt cacgtcatct gcaacgtcta
      541 ggtccaattt gaagtgcgtg ccacttacac acacattcat attgcgaaac tccttaagta
      601 aacgagcatg gtgcaaaaga atgaggccag agacagattt tgatggtatt agggtcctgg
      661 ggtgggcccc ctggagccat gtcctcgctc atatgcagga cattggccct cttacattat
      721 tgaatgccgg gtgctacgta tttgccacga ccccatctac ctacccgacg gagctgaagg
      781 atgaccgtga tgttattagt tgcgccgcaa atgccgtaat gtacaaaggt gttaaatcct
      841 tcgcatgcct cccattcgtg cttgggggac tcaaggcttt atgtgagtca gagcccagtg
      901 ttaaggcaca actgcaagtg gaggaacgag cccagctttt gaagtcactc caacacatga
      961 aaatcctgga atgcgggggg gcaatgctcg aagtgagtgt ggcaagctgg gctatagaga
     1021 ataggattcc tatatctatt gggattggta tgacagagac aggcggcgct cttttcgctg
     1081 ggccggtcca agcaattcaa accggcttta gcagtgaaga taaatttatc gaggacgcaa
     1141 cctacttgct ggtcaaggac gattacgaga gccacgcaga ggaggacata aacgagggcg
     1201 agttagtggt aaagtccaga atgttaccta ggggttattt gggctataac gacccctctt
     1261 tctctgttga cgatgctggt tgggttactt tcaaaactgg agatcgatat tcagtcactc
     1321 ctgacggcaa attctcctgg cttggtcgaa atactgattt cattcagatg acgagtggtg
     1381 agactctcga tccccgtccg attgagtccc tcctttgtga gtccagcctt atatcacgag
     1441 cctgtgtcat cggggataag ttcctgaacg gaccagccac agctgtatgc gctattatag
     1501 aactcgaacc aacgactgtt gagaaaggcc aggctcacag cagggatatt gcccgaatct
     1561 ttgcccccat caatcgagat cttcctcccc cgttacgtat agcatggagt cacgtacttg
     1621 tacttcagcc aagcgagaaa ataccgatga cgaaaaaggg tacaatcttc cgtaagaaaa
     1681 tcgaacaggt tttcggttcc gctttaggag gcagtagcgg cgacaactca caagcaacca
     1741 ctgacgcttc agtagttagg agggatgagt tgagcaacac ggtcaaacat atcataagca
     1801 gagttttggg agtctccgac gatgaactcc tttggacact gagctttgct gagcttggca
     1861 tgacgtccgc tcttgctact agaatagcaa atgaattgaa cgaagttctg gtaggtgtga
     1921 acctccccat caatgcatgc tacattcatg tggacctgcc ttctttgtca aatgccgtgt
     1981 atgcaaagtt agcacacctt aaactcccgg ataggacgcc cgagccaagg aaggccccag
     2041 ttgaaaatcc cggtggaaag gaaatcgtaa tagtaggtca ggcttttagg cttccgggga
     2101 gtataaatga tgtcgcttct ttgagggacg cttttttggc tagacaagca tccagtatta
     2161 ttactgaaat accgccagat agatgggacc acgcatcatt ttatccaaaa gacatacgat
     2221 ttaataaagc agggctcgtc gacatcgcta actacgacca cagttttttc ggattgacag
     2281 ccacggaggc actgtacctg tcaccaacga tgcgtttggc acttgaagtg tcctttgaag
     2341 ccttggagaa tgctaatata cccgtgtccc agttaaaggg ctctcaaaca gctgtatatg
     2401 tcgcaaccac cgacgacggg tttgagacac tgctgaatgc tgaggcagga tatgatgcat
     2461 acacacgatt ctatggcact ggtagggccg cctctacggc cagtggacga attagttatt
     2521 tactcgacgt acacggccct tcaattaccg tagacactgc ttgttcaggc ggtgctgttt
     2581 gcatagacca ggccatagac tacctccaat cctctagcgc agctgatacc gccataatct
     2641 gcgccagcaa cactcactgc tggccaggtt ccttcatgtt tctgagtgct caaggtatgg
     2701 taagttccgg ggggcgatgt gcaactttta cgacggatgc cgacggctat gtaccctctg
     2761 aaggtgctgt ggcatttatc ctcaaaaccc gagaagcagc tatgagagat aaagatacta
     2821 tcttggcaac tatcaaagca acgcagatta gtcataatgg tcgttcccaa ggtttagtcg
     2881 caccaaacgt caattcacaa gctgatttac acagaagctt attgcagaaa gctggtttga
     2941 gtccagccga cattcacttc atcgaagctc acgggacagg aacgtcactc ggtgacctta
     3001 gtgaaataca agcaattaat gatgcctaca cgtcatctca gcctcgaaca gccggaccct
     3061 taatagtaag cgcaagtaaa accgtcattg gtcacacaga gcctgctgga ccgttggtag
     3121 gcatgctctc agttctcaac tcctttaaag agggcgcagt tcccggctta gctcacctga
     3181 cagcagataa cctcaatccg gctttagact gctcctccgt ccctttactt atcccctacc
     3241 agcctgtcca cttggcagct cctaagccac acagggctgc tgtgatgagt tatggcttct
     3301 ccggcactct tggaggtata gttcttgaag caccggatga ggaaaggctt gaggaagaac
     3361 cacccaacga taagcctatg ttatttgttg ttagcgccaa aactcatact gcattgattg
     3421 agtaccttgg acgatacctt gaatttctgc tccaggccaa tccccaagac ttctgcgata
     3481 tctgttacac tagctgcgtt ggacgtgagc attacaggta ccgttttgca tgtgtggcaa
     3541 acgatatgga agatttaatt ggccaacttc agaagaggct tgggtctaag gttccaccta
     3601 aaccgtccta taaaagagga gccctcgctt ttgctttcag tggacaaggg acccagtttc
     3661 gtggaatggc aacagaattg gctaaggcat atagtggttt tcgtaagata gtcagtgatc
     3721 ttgctaaaag agcatcagaa ctgtccggcc atgcaatcga caggtttctg cttgcatatg
     3781 acattggggc tgagaatgtt gcaccggata gtgaagctga tcaaatctgc atattcgttt
     3841 accaatgtag tgttctcaga tggctccaaa caatggggat tcgtccgtca gccgtgatcg
     3901 gtcacagcct cggtgagatt agcgcatccg tagctgctgg agcattgtcc ttggactctg
     3961 cccttgacct cgtaataagt agagccaggt tactgaggtc atctacgaat gccccagctg
     4021 gcatggcagc tatgtctgca tcccaggatg aagtagtcga actcatagga aagctcgatt
     4081 tagataaagc taatagcctc agcgtgtccg taataaacgg cccccagaac accgttgttt
     4141 caggatcttc tgcagcaata gaaagtatcg ttgctctcgc aaagggacga aaaatcaagg
     4201 ccagcgcact caacattaac caggcattcc atagtcctta cgtcgattct gcagtacccg
     4261 gcttacgagc ttggagtgag aaacatatat catcagctcg accattgcaa atccctttgt
     4321 actctaccct cttgggcgct caagtttctg agggtcaaat gttaaatccc gatcactggg
     4381 tagaccatgc taggaaaccc gttcagttcg cacaagcagc cactataatg aaggaatctt
     4441 tcaccggagt aataatcgat attggacctc aagttgtagc atggtccctg ctcttaagca
     4501 atggtcttac ttccgtgacc gcactcgccg caaagcgtgg ccgttcacag caagtggcct
     4561 tcctctccgc cttagctgat ctttatcagg actacggcgt cgtaccagac tttgttggac
     4621 tgtacgcaca acaggaggac gcttccagac tgaaaaagac cgatatctta acttacccct
     4681 ttcagagggt tcgacgttac ccttccttta taccctctag aagggcaccc acccacgctc
     4741 atgtccaaga cgaggaaact ctgtcatcag gatcatcaac tccaacctta gaaaatactg
     4801 acctggacag tgggaaggaa tctctgatgg gaccaacaag agggcttttg cgagtcgatg
     4861 atcttaggga ctcaatagtc agttcagtca aagatgtcct ggaattgaag agtaacgaag
     4921 atctggacct cagcgagtcc cttaatgctc ttggcatgga ctctataatg ttcgctcaat
     4981 tacgaaaaag gataggggag ggcctcggcc tgtccgttcc aatggttttt ctcagcgatg
     5041 ccttcagtat tggagaaatg gtcagtaacc tcgtggaaca ggccgaagct tccgaggaca
     5101 attgagcttt gagaccacga agtggctctt cagtggacga aagggcctcg tgatacgcct
     5161 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     5221 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     5281 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgcg
     5341 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     5401 tttcatggct tgttatgact gttttttttt ggggtacagt ctatgcctcg ggcatccaag
     5461 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     5521 cagcagggca gtcgccctaa aacaaagtta aacatcatga gggaagcggt gatcgccgaa
     5581 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
     5641 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagcgatatt
     5701 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
     5761 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
     5821 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
     5881 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
     5941 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
     6001 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
     6061 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
     6121 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
     6181 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga
     6241 caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa
     6301 tttgtccatt acgtaaaagg cgagatcacc aaggtagtcg gcaaataact gtcagaccaa
     6361 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6421 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6481 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6541 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6601 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6661 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6721 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6781 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6841 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6901 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6961 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7021 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7081 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7141 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     7201 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     7261 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     7321 cgttggccga ttcattaatc actctgt
//
