LOCUS       sequence-292653-        9999 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  OsTIR1(WT)-P2A-mAID-EGFP-NLS TOL2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9999)
  AUTHORS   Yesbolatova A, Saito Y, Kitamoto N, Makino-Itou H, Ajima R, Nakano 
            R, Nakaoka H, Fukui K, Gamo K, Tominari Y, Takeuchi H, Saga Y, 
            Hayashi KI, Kanemaki MT
  TITLE     The auxin-inducible degron 2 technology provides sharp degradation 
            control in yeast, mammalian cells, and mice.
  JOURNAL   Nat Commun. 2020 Nov 11;11(1):5701. doi: 10.1038/s41467-020-19532-z.
  PUBMED    33177522
REFERENCE   2  (bases 1 to 9999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-11-11"; volume: "11(1):5701. doi"; pages: " 
            10.1038/s41467-020-19532-z"
COMMENT     Sequence Label: pAY7 (OsTIR1 mAID-EGFP-NLS TOL2)
FEATURES             Location/Qualifiers
     source          1..9999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(678..689,690..852)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    complement(883..916)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(1473..2072)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     2053..2072
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(2112..2611)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2160..2179)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     2553..2576
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    complement(2672..2705)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    2732..2853
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2743..2762)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2797..2816
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3004..3024)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(3061..3771,3772..3774,3775..3777))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(3061..3771,3772..3774,3775..3777))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3103..3124)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3452..3471
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3711..3732
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(3784..3987)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             complement(3994..4050)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(4066..5790)
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     primer_bind     complement(5835..5854)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          5862..6878
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(5916..5938)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(6879..7156)
                     /label=chicken beta-actin promoter
     enhancer        7158..7537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    join(7559..7746,7747..7758)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7815..7835)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7815..7833)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7854..7870)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7854..7870)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7867..7889)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7878..7894
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7902..7908,7909..7926,7927..7932))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7947..7968
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8085..8102)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8256..8844)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8336..8355)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9015..9806,9807..9875))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9638..9657
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9876..9980)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc ggcgggtgtg gtggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac
      661 cggcatatgg ttcttgacag aggtgtaaaa agtactcaaa aattttactc aagtgaaagt
      721 acaagtactt agggaaaatt ttactcaatt aaaagtaaaa gtatctggct agaatcttac
      781 ttgagtaaaa gtaaaaaagt actccattaa aattgtactt gagtattaag gaagtaaaag
      841 taaaagcaag aaagatctta gtgaacctct tcgagggacc taataacttc gtatagcata
      901 cattatacga agttatatta agggttccgg atctcgacca gcttctgatg gaattagaac
      961 ttggcaaaac aatactgaga atgaagtgta tgtggaacag aggctgctga tctcgttctt
     1021 caggctatga aactgacaca tttggaaacc acagtactta gaaccacaaa gtgggaatca
     1081 agagaaaaac aatgatccca cgagagatct atagatctat agatcatgag tgggaggaat
     1141 gagctggccc ttaatttggt tttgcttgtt taaattatga tatccaacta tgaaacatta
     1201 tcataaagca atagtaaaga gccttcagta aagagcaggc atttatctaa tcccacccca
     1261 cccccacccc cgtagctcca atccttccat tcaaaatgta ggtactctgt tctcaccctt
     1321 cttaacaaag tatgacagga aaaacttcca ttttagtgga catctttatt gtttaataga
     1381 tcatcaattt ctgcatcccg gggatctgat atcatcgatg catggggtcg tgcgctcctt
     1441 tcggtcgggc gctgcgggtc gtggggcggg cgtcaggcac cgggcttgcg ggtcatgcac
     1501 caggtgcgcg gtccttcggg cacctcgacg tcggcggtga cggtgaagcc gagccgctcg
     1561 tagaagggga ggttgtgggg cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg
     1621 gccgcctcca ctccggggag cacgacggcg ctgcccagac ccttgccctg gtggtcgggc
     1681 gagacgccga cggtggccag gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg
     1741 ccttccatct gttgctgcgc ggccagccgg gaaccgctca actcggccat gcgcgggccg
     1801 atctcggcga acaccgcccc cgcttcgacg ctctccggcg tggtccagac cgccaccgcg
     1861 gcgccgtcgt ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt
     1921 tcttgcagct cggtgacccg ctcgatgtgg cggtccggat cgacggtgtg gcgcgtggcg
     1981 gggtagtcgg cgaacgcggc ggcgagggtg cgtacggccc tggggacgtc gtcgcgggtg
     2041 gcgaggcgca ccgtgggctt gtactcggtc atggtaagct tcagctgctc gagatctaga
     2101 tggatgcagg tcgaaaggcc cggagatgag gaagaggaga acagcgcggc agacgtgcgc
     2161 ttttgaagcg tgcagaatgc cgggcctccg gaggaccttc gggcgcccgc cccgcccctg
     2221 agcccgcccc tgagcccgcc cccggaccca ccccttccca gcctctgagc ccagaaagcg
     2281 aaggagcaaa gctgctattg gccgctgccc caaaggccta cccgcttcca ttgctcagcg
     2341 gtgctgtcca tctgcacgag actagtgaga cgtgctactt ccatttgtca cgtcctgcac
     2401 gacgcgagct gcggggcggg ggggaacttc ctgactaggg gaggagtaga aggtggcgcg
     2461 aaggggccac caaagaacgg agtcggttgg cgcctaccgg tggatgtgga atgtgtgcga
     2521 ggccagaggc cacttgtgta gcgccaagtg cccagcgggg ctgctaaagc gcatgctcca
     2581 gactgccttg ggaaaagcgc ctcccctacc cggtagaatt tcgaggtcga gatcctaagc
     2641 ttggctggac gtaaactcct cttcagacct aataacttcg tatagcatac attatacgaa
     2701 gttatattaa gggttattga atatgatcgc ttaagataca ttgatgagtt tggacaaacc
     2761 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     2821 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     2881 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     2941 ggtatggctg attatgatca gttatctaga tccggtggat cccgggcccg cggtaccgtc
     3001 gatgaccttt cttttcttct tggggtcgac tgcagaattc gaagcttggg cgcctgcacc
     3061 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
     3121 catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc tcaggtagtg
     3181 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
     3241 gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt
     3301 cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg
     3361 ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag
     3421 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa
     3481 gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt
     3541 catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg tcacgagggt
     3601 gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta
     3661 ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc
     3721 cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatgct
     3781 agctttatac atcctcaaat cgattttcct caagtacggt gctccgtcca ttgatacctt
     3841 cacgaacgcc gccgcctccg ggccaccgct tgatttttgg caggaaacca tcacgttctt
     3901 ccggtatgat ctcaccggtg gccatcccac aacttgtgcc ttggccggag gtttggctgg
     3961 atctttagga caagcactct tctccttaga tctaggtcca gggttctcct ccacgtctcc
     4021 agcctgcttc agcaggctga agttagtagc tccgcttcct gatctcagaa tcttcacaaa
     4081 gttgggagca tcatctcttg cccctgcggt ggtccgatac acatacagtt tctccacctt
     4141 tggcaggtcg ccatgatttt cctccatttc gttagacccg tcccgctcat taatgacttc
     4201 cacgttcagc ataggcattt tactagccag gacctgacag cccttcagag tcacattgca
     4261 actagacatc cacaggctcc tcattgtctc gtatctggca aagtttccca gcagagcggc
     4321 atccccgaaa gggctgtccc ggatttccag ttttcgcaga ttcttacacc cgttcatcac
     4381 gtgcatcatg cccttatcgg agtctccggc aaatgcaatg ctcagcatct ccagctgctt
     4441 agcatacttg ccgatgtaca tgaacacttt gtctgtcagc aggccggaaa tgctcagcct
     4501 ctggagtccc ttgcactctc tcacgatagc tccaaaccct tcatccagtg gctgggatgt
     4561 gaccacgtcg ggtttgcctg gttccagaat acacagccga aatcgggtga aattagggca
     4621 gttcttggcg acggtcacca gtgcagcatt agtcatctga tgacagaaat acagcaggct
     4681 gttcagcttt gggcatccca gtgagactgc caccaggcct tcctcagtga cagcactata
     4741 tccggccacg taaaaatcag atgggaacac tctcagctcc tggaggtcct tgcaggagct
     4801 ggcgaccacc tggagcccct tatcggaaat acagtccagc acccacagtc gctggagttt
     4861 cacgcagcgt gagatcatct ttgtcagatc actggcgtcc agggttggtg catagctcag
     4921 gttcaggcct gtcagctgtg cacacagagg gtagataaag ctcaggcaca cgggtgaagc
     4981 atcccagaat cctgacagac tcctcagcat tttacacttc tccagggcag aagtcagttt
     5041 aaagtaggat tctgtctgga aatcgtctgt cagattgccg gttcccagat cctccaggtt
     5101 aggggttctc agcagaatct tagccagagt gtccacgctc acgctccggt tcagccgcag
     5161 agacctcagg ttggggcttc ttgagaccag ccgctccagg gacccagcat tcacttcgcc
     5221 tttgatgcag gcaaagttca gggacaccag gctggtacat gagtctggga agcaagacag
     5281 ccatctgggg cctctatcct ccacttcatt ctcctggagg tccagctccc gcagcagctt
     5341 acagtgggag gccacggctg ccagtccatc ggtgctgaag ccttcgcaag aaatcagcac
     5401 cagtgcgcga aacctaggga agctccgagc cagcagctcc agactttcgt cagagaccac
     5461 cattcgcttc atgcgcagtt cctccagtcc atgacagccg cgagcggctg cctcgatcca
     5521 agggccggcg tatcctcccc aatcaggggg caccaggtta aagtcggcga agtggggttt
     5581 ccccttgacg gtcagtgcgc gcacatttgg aaacctagcg gcgaccctgc ctgctctcac
     5641 agcatagcag ttgccgacga acactcctct ccggctcagg cgttcgatct cgtaccacac
     5701 tttgcagacc aggctcactg tgtttctatc tctctgtgca ggcaggaagc taaaaatgtg
     5761 ttcgacgacc tcttcaggaa agtatgtcat ggtggcagcg cttcgaggaa ttctttgcca
     5821 aaatgatgag acagcacaat aaccagcacg ttgcccagga gctgtaggaa aaagaagaag
     5881 gcatgaacat ggttagcaga ggctctagag ccgccggtca cacgccagaa gccgaacccc
     5941 gccctgcccc gtcccccccg aaggcagccg tccccccgcg gacagccccg aggctggaga
     6001 gggagaaggg gacggcggcg cggcgacgca cgaaggccct ccccgcccat ttccttcctg
     6061 ccggcgccgc accgcttcgc cccgcgcccg ctagaggggg tgcggcggcg cctcccagat
     6121 ttcggctccg cacagatttg ggacaaagga agtccctgcg ccctctcgca cgattaccat
     6181 aaaaggcaat ggctgcggct cgccgcgcct cgacagccgc cggcgctccg ggggccgccg
     6241 cgcccctccc ccgagcccyc cccggcccga ggcggccccg ccccgcccgg cacccccacc
     6301 tgccgccacc ccccgcccgg cacggcgagc cccgcgccac gccccgtacg gagccccgca
     6361 cccgaagccg ggccgtgctc agcaactcgg ggaggggggt gcaggggggg ttgcagcccg
     6421 accgacgcgc ccacaccccc tgctcacccc cccacgcaca caccccgcac gcagcctttg
     6481 ttcccctcgc agcccccccc gcaccgcggg gcaccgcccc cggccgcgct cccctcgcgc
     6541 acactgcgga gcgcacaaag ccccgcgccg cgcccgcagc gctcacagcc gccgggcagc
     6601 gcggagccgc acgcggcgct ccccacgcac acacacacgc acgcaccccc cgagccgctc
     6661 cccccgcaca aagggccctc ccggagcccc tcaaggcttt cacgcagcca cagaaaagaa
     6721 acaagccgtc attaaaccaa gcgctaatta cagcccggag gagaagggcc gtcccgcccg
     6781 ctcacctgtg ggagtaacgc ggtcagtcag agccggggcg ggcggcgcga ggcggcggcg
     6841 gagcggggca cggggcgaag gcagcgcgca gcgactcccg cccgccgcgc gcttcgcttt
     6901 ttatagggcc gccgccgccg ccgcctcgcc ataaaaggaa actttcggag cgcgccgctc
     6961 tgattggctg ccgccgcacc tctccgcctc gccccscccc sccccycscc ccsccccscc
     7021 ccsccyggcg cgcgcccccc cccccccccc gcccccatcg ctgcacaaaa taattaaaaa
     7081 ataaataaat acaaaattgg gggtggggag gggggggaga tggggagagt gaagcagaac
     7141 gtggggctca cctcgaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
     7201 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
     7261 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
     7321 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
     7381 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
     7441 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
     7501 attgattact attaataact agtcaataat caatgtcgag catgcatcta gagggcccaa
     7561 gtgatctcca aaaaataagt actttttgac tgtaaataaa attgtaagga gtaaaaagta
     7621 cttttttttc taaaaaaatg taattaagta aaagtaaaag tattgatttt taattgtact
     7681 caagtaaagt aaaaatcccc aaaaataata cttaagtaca gtaatcaagt aaaattactc
     7741 aagtacttta cacctctggt tcttgacccc ctaccttcag caagcccagc agatccgagc
     7801 tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
     7861 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     7921 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     7981 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     8041 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     8101 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     8161 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8221 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8281 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8341 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     8401 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     8461 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8521 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8581 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8641 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     8701 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8761 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     8821 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8881 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     8941 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     9001 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     9061 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9121 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     9181 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9241 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9301 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     9361 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     9421 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9481 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     9541 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9601 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     9661 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     9721 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     9781 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9841 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9901 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9961 aaacaaatag gggttccgcg cacatttccc cgaaaagtg
//
