LOCUS       sequence-292654-        3120 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  individual component of Fungal Bioluminescent Pathway, MoClo level 0
            plasmid, NnCPH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-03-25"; volume: "9. pii: 52786. doi"; pages: " 
            10.7554/eLife.52786"
COMMENT     Sequence Label: pCO602
FEATURES             Location/Qualifiers
     source          1..3120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(945..963)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1031..1135
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1357..2148
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2319..2907
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2808..2827
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3061..3078
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgttggccga ttcattaatc actctgtggt ctcaaatggc tcctatctcc tccacctggt
       61 ccagattaat caggttcgta gctatagaaa cgtcactcgt gcatataggc gaaccgattg
      121 atgcaactat ggacgtaggg cttgctcgtc gagaagggaa aactattcag gcctatgaga
      181 ttatcggatc tggcagcgcc ctggaccttt ctgcacaggt gagtaagaac gtgctcacag
      241 tacgagaatt gttgatgcca ctttctagag aagagataaa gacggtcaga tgcttaggcc
      301 ttaactatcc tgtccacgca acagaagcca atgttgctgt accgaagttt ccgaacctgt
      361 tctacaaacc agtcacaagt ctcatcgggc ctgatggtct tatcacgatc cctagtgtag
      421 tgcaaccgcc taaggaacat cagtcagatt atgaagctga acttgtgatt gtaatcggaa
      481 aggccgccaa gaacgtgtct gaggacgaag ccttggatta tgtcttaggc tatacagctg
      541 ctaacgatat tagtttccga aaacaccagc tcgcagtctc acagtggtca ttctcaaaag
      601 gatttggatc tctccttctc actatacgta tggcccagac acatagtgga aacataaata
      661 ggttctcacg tgatcaaatc tttaatgtta agaaaacaat cagctttttg tctcaaggaa
      721 ctaccctcga acctgggtct atcattttga ctggcacccc tgatggggtc ggcttcgtca
      781 ggaacccgcc cttatactta aaagatgggg atgaagtgat gacttggata ggttccggca
      841 ttggtacgct tgctaacacc gttcaagaag agaaaacatg ctttgcaagc ggtgggcatg
      901 agtaggcttt gagaccacga agtggctctt cagtggacga aagggcctcg tgatacgcct
      961 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     1021 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     1081 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgcg
     1141 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     1201 tttcatggct tgttatgact gttttttttt ggggtacagt ctatgcctcg ggcatccaag
     1261 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     1321 cagcagggca gtcgccctaa aacaaagtta aacatcatga gggaagcggt gatcgccgaa
     1381 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
     1441 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagcgatatt
     1501 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
     1561 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
     1621 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
     1681 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
     1741 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
     1801 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
     1861 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
     1921 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
     1981 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga
     2041 caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa
     2101 tttgtccatt acgtaaaagg cgagatcacc aaggtagtcg gcaaataact gtcagaccaa
     2161 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     2221 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     2281 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2341 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2401 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2461 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2521 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2581 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2641 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2701 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2761 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2821 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     2881 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     2941 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     3001 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     3061 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
//
