LOCUS       sequence-292655-        3054 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  individual component of Fungal Bioluminescent Pathway, MoClo level 0
            plasmid, NnLuz.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-03-25"; volume: "9. pii: 52786. doi"; pages: " 
            10.7554/eLife.52786"
COMMENT     Sequence Label: pCO603
FEATURES             Location/Qualifiers
     source          1..3054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(852..870)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        938..1042
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1264..2055
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2226..2814
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2715..2734
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2968..2985
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcaaat gcgtataaat atctctctgt ctagcttatt cgagagatta agcaaactca
       61 gctctcgtag catagctatt acatgtggtg tcgtcctcgc ctctgctatt gcttttccca
      121 tcatacgacg agattatcag acgttcttag aggttggtcc tagctatgcc cctcaaaatt
      181 ttcgaggcta cattatcgtt tgcgtactca gcttgttccg acaagagcaa aaagggttgg
      241 caatctacga cagactccca gagaagagaa gatggctggc cgatctccca tttcgtgaag
      301 gtactagacc tagtataaca tcacacataa tacagagaca gcgaactcaa cttgtagacc
      361 aagagtttgc cacacgagaa ctgatagaca aagttattcc cagggtgcaa gcacgacata
      421 cagataaaac atttttgagc acgagcaagt ttgaatttca tgctaaagcc attttcctgt
      481 tgccaagtat tcctataaac gaccccttaa acatacctag tcatgatacg gttcgtagaa
      541 cgaaacgaga gattgcacac atgcatgact accacgactg tactcttcac ttagctcttg
      601 cagctcaaga tggaaaagaa gtcctgaaaa aaggctgggg ccaacgtcat ccgcttgccg
      661 ggccgggtgt acctgggccg ccgacagaat ggactttctt atacgcaccc cgtaatgagg
      721 aagaagctag ggtagtagaa atgattgtcg aggcaagtat cggatacatg actaatgatc
      781 cggcaggtaa aatcgtcgag aatgccaaat gagctttgag accacgaagt ggctcttcag
      841 tggacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
      901 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      961 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     1021 ataatattga aaaaggaaga gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     1081 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttttggg
     1141 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
     1201 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
     1261 atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
     1321 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
     1381 ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt aaggcttgat
     1441 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
     1501 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
     1561 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
     1621 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
     1681 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
     1741 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
     1801 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
     1861 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
     1921 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
     1981 gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga gatcaccaag
     2041 gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     2101 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     2161 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2221 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2281 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2341 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     2401 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2461 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2521 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2581 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2641 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2701 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2761 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2821 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2881 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2941 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3001 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact ctgt
//
