LOCUS       sequence-292656-        3519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  individual component of Fungal Bioluminescent Pathway, MoClo level 0
            plasmid, NnH3H.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-03-25"; volume: "9. pii: 52786. doi"; pages: " 
            10.7554/eLife.52786"
COMMENT     Sequence Label: pCO604
FEATURES             Location/Qualifiers
     source          1..3519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1317..1335)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1403..1507
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1729..2520
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2691..3279
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3180..3199
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3433..3450
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcaaat ggcatccttt gaaaattcac tcagtgtctt aatagttggg gctggcttag
       61 gtggtttggc cgctgctata gcactgcgaa ggcaaggaca tgtagtcaag atatacgata
      121 gttcttcatt caaggccgaa ttgggagcag gattagctgt accacccaat actctccgtt
      181 ccctgcaaca actgggctgt aatacagaaa acctgaacgg agtagacaac ctctgcttta
      241 cggccatggg atacgacggg agtgtaggta tgatgaataa tatgaccgac taccgtgagg
      301 catacggcac ttcctggata atggtacatc gagtggatct ccacaacgag ctgatgcgag
      361 tcgccttgga tccgggcggg ttagggcctc ctgccactct tcaccttaac catcgtgtta
      421 cgttctgcga tgtggatgcc tgtacagtca cgtttacgaa cgggacgaca cagtctgctg
      481 acttgattgt cggagctgat ggtattaggt ctacgattcg aagattcgtt ctggaggagg
      541 acgtgactgt tccagctagc ggaattgtcg gctttcgatg gttggtccag gccgatgctc
      601 tcgacccata tcccgaactg gattggattg tcaaaaaacc acccttggga gccaggttga
      661 tttctactcc acagaacccg cagagtggcg taggactggc cgatagacga acgatcatta
      721 tttacgcatg cagaggtggg accatggtta atgtattagc cgtacacgac gacgaaagag
      781 atcaaaacac tgctgattgg tccgttcccg catcaaagga tgatttattc agagtgtttc
      841 acgattacca tccaagattc cgacgactct tagagttagc acaggacatt aatttatggc
      901 agatgagggt ggtcccggtc ttgaaaaaat gggtgaacaa gagggtatgc ttgctgggtg
      961 atgccgccca tgcttcactg cccacacttg gacaaggctt cgggatgggg ttggaggatg
     1021 cagtcgcttt gggcacgttg ttgcctaagg gaacgaccgc atcacagatc gagacaaggc
     1081 tggccgttta cgaacagctg cgaaaggatc gagctgagtt tgtcgccgca gagagctatg
     1141 aggagcaata tgtcccagag atgagagggc tgtaccttag gtccaaagaa ttgagagaca
     1201 gagtgatggg ttatgatatt aaggtagagt cagagaaggt tttggaaaca ctgttgcgat
     1261 catccaactc agcttgagct ttgagaccac gaagtggctc ttcagtggac gaaagggcct
     1321 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     1381 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     1441 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     1501 gaagagtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     1561 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ttggggtaca gtctatgcct
     1621 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
     1681 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gagggaagcg
     1741 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc
     1801 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca
     1861 cacagcgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga
     1921 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc
     1981 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag
     2041 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca
     2101 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc
     2161 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag
     2221 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga
     2281 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg
     2341 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata
     2401 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat
     2461 cagttggaag aatttgtcca ttacgtaaaa ggcgagatca ccaaggtagt cggcaaataa
     2521 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     2581 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     2641 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2701 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2761 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2821 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     2881 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     2941 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3001 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3061 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3121 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3181 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3241 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3301 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     3361 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     3421 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     3481 cctctccccg cgcgttggcc gattcattaa tcactctgt
//
