LOCUS       sequence-292658-        3291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  individual component of Fungal Bioluminescent Pathway, MoClo level 0
            plasmid, AsNPGA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-03-25"; volume: "9. pii: 52786. doi"; pages: " 
            10.7554/eLife.52786"
COMMENT     Sequence Label: pCO605
FEATURES             Location/Qualifiers
     source          1..3291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1116..1134)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1202..1306
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1528..2319
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2490..3078
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2979..2998
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3232..3249
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgttggccga ttcattaatc actctgtggt ctcaaatggt gcaggacact tcaagtgcta
       61 gcacaagtcc aattttaacc agatggtata tagacactcg acctctcacg gcaagtaccg
      121 ccgcacttcc gctgctggaa acgctgcaac cagctgatca gatctccgtc caaaaatatt
      181 atcatctcaa ggacaagcac atgtcacttg cctccaacct tttgaagtat ttattcgttc
      241 atcgaaattg ccgaatacca tggagtagta ttgtcatatc aagaacgccc gacccgcaca
      301 gaaggccttg ctatatacct ccttcaggtt cacaagagga ctccttcaaa gacggatata
      361 cgggaatcaa cgtagaattc aacgttagtc accaagccag catggtggca atcgcaggta
      421 cggctttcac acctaatagc ggtggagata gcaaactcaa accggaggtc ggaatagata
      481 taacatgtgt aaatgagagg cagggcagga atggagaaga aagatccttg gaatccctga
      541 ggcagtatat agatatattc agcgaagtat tcagcaccgc agagatggct aatataaggc
      601 gacttgatgg ggtttcctca agttccctta gtgcagacag actcgtagat tatggatacc
      661 gattattcta cacctattgg gccttaaaag aggcttatat caagatgaca ggtgaggcct
      721 tattggctcc ttggttgaga gagctggagt tttccaacgt ggtcgctcca gccgctgtcg
      781 ccgaaagcgg ggactctgct ggagactttg gcgaaccgta cactggcgtg cgtacaacat
      841 tgtataagaa cctcgtagag gacgtacgaa ttgaggtagc tgctttggga ggagattatc
      901 tgttcgcaac agccgctaga ggtggtggta taggcgcatc ctcacgtcct ggtggtggcc
      961 cagatggctc tggaattaga tcccaggacc cttggaggcc gttcaagaaa ctcgatatag
     1021 agcgtgacat acagccatgt gctacgggcg tttgtaattg cctgtcctca ggataggctt
     1081 tgagaccacg aagtggctct tcagtggacg aaagggcctc gtgatacgcc tatttttata
     1141 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     1201 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     1261 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa
     1321 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     1381 ttgttatgac tgtttttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     1441 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     1501 agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact
     1561 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     1621 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagcgatat tgatttgctg
     1681 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     1741 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     1801 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     1861 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     1921 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     1981 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     2041 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     2101 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     2161 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     2221 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccat
     2281 tacgtaaaag gcgagatcac caaggtagtc ggcaaataac tgtcagacca agtttactca
     2341 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     2401 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     2461 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     2521 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     2581 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     2641 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     2701 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     2761 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2821 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     2881 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     2941 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     3001 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     3061 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     3121 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     3181 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     3241 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc g
//
