LOCUS       sequence-292659-        4355 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Individual component of Caffeic Acid biosynthesis pathway, hpaB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-03-25"; volume: "9. pii: 52786. doi"; pages: " 
            10.7554/eLife.52786"
COMMENT     Sequence Label: P525
FEATURES             Location/Qualifiers
     source          1..4355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        964..1309
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1252..1272
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      2988..3188
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     promoter        3263..3367
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gctcatgatc ggcgcgccct atggaggtca acatggtgga
      901 gcacgacact ctggtctact ccaaaaatgt caaagataca gtctcagaag atcaaagggc
      961 tattgagact tttcaacaaa ggataatttc gggaaacctc ctcggattcc attgcccagc
     1021 tatctgtcac ttcatcgaaa ggacagtaga aaaggaaggt ggctcctaca aatgccatca
     1081 ttgcgataaa ggaaaggcta tcattcaaga tctctctgcc gacagtggtc ccaaagatgg
     1141 acccccaccc acgaggagca tcgtggaaaa agaagaggtt ccaaccacgt ctacaaagca
     1201 agtggattga tgtgacatct ccactgacgt aagggatgac gcacaatccc actatccttc
     1261 gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacac gctcgagtat
     1321 aagagctcat ttttacaaca attaccaaca acaacaaaca acaaacaaca ttacaattac
     1381 atttacaatt atcgatacaa tgaaacccga ggacttcagg gcctcaacac aaagaccctt
     1441 tacaggtgaa gaatacctta agtctctcca ggacggacga gagatatata tatacgggga
     1501 aagggtcaaa gacgttacaa cccatcccgc cttcagaaat gctgccgcct ctgtggctca
     1561 gctctatgat gccttgcata aaccggaaat gcaagactca ttatgttgga atacggatac
     1621 tggctctggc ggttacaccc ataaattctt tagagtcgca aagtcagctg acgatttacg
     1681 tcagcagcga gatgcaattg ccgagtggtc caggttgtct tatggttgga tggggcgtac
     1741 ccctgactat aaggccgcct ttggatgtgc cttgggtgca aacccagggt tttatggcca
     1801 gttcgagcag aatgcccgaa actggtacac caggatccaa gaaaccgggc tctacttcaa
     1861 ccacgcaatc gttaatcctc cgatcgacag acacctccct acggacaagg tgaaagatgt
     1921 ttatataaag ttggagaaag agacagatgc aggaattatc gttagcggag ctaaggtcgt
     1981 ggccactaac tccgctctca cacactataa tatgatcgga tttggatctg ctcaagtcat
     2041 gggagaaaat cctgactttg ctcttatgtt tgttgctcca atggatgctg atggcgttaa
     2101 attgatctca cgagcttcct acgaaatggt agctggggcc acgggtagtc cctacgacta
     2161 tcccttgtcc tcccgatttg atgaaaatga tgccatcttg gtcatggaca atgtccttat
     2221 tccatgggaa aacgttttga tctaccgaga tttcgaccga tgtaggcgat ggaccatgga
     2281 gggcggcttc gccagaatgt accctttaca agcatgcgtc cgtcttgccg ttaaactcga
     2341 ctttataacc gccttgttga aaaagtcatt agaatgcacc ggcaccctcg aatttcgagg
     2401 agtccaagct gacctggggg aggttgtggc atggcgaaac acattttggg ccctcagtga
     2461 ctcaatgtgc agcgaggcca ctccgtgggt aaacggcgcc tatctgccgg accatgccgc
     2521 attgcaaacg taccgtgtgc tcgcacctat ggcctatgct aagataaaga acattataga
     2581 acgtaatgtt acaagcggac tgatctatct cccaagctcc gccagggatc tcaacaatcc
     2641 ccaaatagac caatatcttg caaaatacgt aaggggatca aacgggatgg atcacgttca
     2701 aaggattaaa atcttaaagc tcatgtggga cgcaatcggg tctgagttcg gtgggagaca
     2761 cgagctttac gagattaact atagcggttc tcaggatgag ataaggcttc agtgtctgag
     2821 acaggcacaa aacagtggca atatggataa aatgatggca atggtcgata ggtgtttgag
     2881 tgagtacgac caggatggat ggacggtccc ccatctccac aataatgatg acattaatat
     2941 gttagataaa ttgctgaagt ccggctgagc ttgatccccc gtcgacagct agctatatca
     3001 tcaatttatg tattacacat aatatcgcac tcagtctttc atctacggca atgtaccagc
     3061 tgatataatc agttattgaa atatttctga atttaaactt gcatcaataa atttatgttt
     3121 ttgcttggac tataatacct gacttgttat tttatcaata aatatttaaa ctatatttct
     3181 ttcaagatcg ctgttagagc tcggacgtgg gcaggtgtga tgaaaagcct tagggtcagg
     3241 tggcactttt cggggaaatg tgcgatgaac ccctatttgt ttatttttct aaatacattc
     3301 aaatatgtat ccgctaatga gacaataacc ctgataaatg cttcaattat attgaaaaag
     3361 gaagagtatg agtatccaac atttccgtgt cgcccttatt cccttttttg cggccttctg
     3421 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     3481 gggtgcccga gtgggttaca ttgaactgga tctcaacagc ggcaaaatct tagagagttt
     3541 tcgccccgaa gaacgcttcc caatgatgag cactttcaaa gttctgctat gtggcgcggt
     3601 gttatcccgt attgatgctg ggcaagagca actcggtcgc cgcatacact attctcagaa
     3661 tgacttggtt gagtattcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     3721 agaattatgt agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     3781 aacgattgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     3841 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     3901 cacgatgcct gtagcaatgg caacaactct ccgcaaacta ttaactggcg aactacttac
     3961 tcttgcttca cgccagcaac tcattgactg gatggaggcg gacaaagttg caggaccact
     4021 tctgcgctcg gcacttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     4081 tggctctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     4141 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     4201 aggtgcctca ctgattaagc attggtaact gtcagacgaa gggaaataaa tagtagcccg
     4261 cctgatgtgc gggctttttt ttggtggtgg ttaccagtgt cgcttagcta ccgtaggtcc
     4321 tcttaggcgg tccgattagc gacatagtca tcaat
//
