LOCUS       sequence-292663-        5658 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dinoflagellate backbone with rifampicin resistant gene optimized for
            Oxyrrhis marina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Sprecher BN, Zhang H, Lin S
  TITLE     Nuclear Gene Transformation in the Dinoflagellate Oxyrrhis marina.
  JOURNAL   Microorganisms. 2020 Jan 16;8(1). pii: microorganisms8010126. doi: 
            10.3390/microorganisms8010126.
  PUBMED    31963386
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microorganisms."; date: "2020-01-16"; volume: "8(1). pii: 
            microorganisms8010126. doi"; pages: " 10.3390/microorganisms8010126"
COMMENT     Sequence Label: DinoIII-arrO-N
FEATURES             Location/Qualifiers
     source          1..5658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2187..2209
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2206..2222
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5264..5281)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5264..5280)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5273..5295)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5489..5508)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5608..5630
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt gcatgcctgc aggtcgacga ttcccgtagc cattttggct
     2281 caaggtacaa gtcgggctga tgcggtcacg caggcctctt ttgtatcaga taagacagca
     2341 gacgtacatg atataaatat ttattatcgc gatgctccat ctgcaaaatt ccacttggca
     2401 gagaagattt tcaatgcatg aaaacctcgc agaaccaagc accaatgtca gatcatgtat
     2461 gagtcctagg cgccagtaag tctttcaatt gggtgatggc cacattacct gttctcttcc
     2521 tgagggatgc agaatgtggt gtgagtggtt gccgaatgct gtgctgaaca tgtagatggg
     2581 ctgccccagc caagtggata acctacaagg actgcaggat gtagatccag cagtcctggt
     2641 agataatgat tcagacaccg gctcaggctg gcaacagagc aggcaaacct gaacactatc
     2701 gcgcagtggt gggaatacag cccagaatgg gaagcaacct ggcctcttca gccagccatg
     2761 accacaagca actctgcagt tttgcaacac gaaatctttc agggtctgct ccctgtgctt
     2821 acacatattc aaagatattc ttctgcgcat ttcaatgcaa attcttggcg atgattcgct
     2881 tccgcaggta tagtgttact agtatataag tctaatcata tacttcaatt tattagtaat
     2941 attgtgaatt tctaggcaag aaggatgcct ggactctgaa ttattatggc attgagtaga
     3001 atctggatct tgatgattat gcattaatat cttgaaatgc atttggattc ccttcgggga
     3061 tcatccgtta aaattggaac gatacagaga agattagcat ggcccctgcg caaggatgac
     3121 acgcacaaat cgagaagtgt aaacaatttt tttgaaatta attgccactt tattttgaat
     3181 acctgaatat gcaggtgaag tagtataagg tattcattat cattgcgatt ttctagtaag
     3241 tcccgtggct gtcatatcta gtaaccctca cctggcaggt ggggaaaagg cgaagacaaa
     3301 tagaatcaaa tagaatgtat gtgctgacgt agggctacat tacctgaggc ttgaggaacc
     3361 tgtgggtatg aatgctactg ttggcagcat ccagtaggtt cagaaacaca cgtccccacg
     3421 taattttttg catgtctaaa tgcactgtga tgattgatga tgattgattc cttgaaggcc
     3481 atggtgggtt actctgtggc ctcttgccac tgacttccag gaaaacgcgg attttctgac
     3541 catcacaagg gaccactacg agaagtatgc agcgatctca gcagagttca tttactgacc
     3601 attctcagga agatcgctcg ggatcacttg ggtaatattg ctttcttcgc agatgttttt
     3661 tccctattaa gctttttgag tcccctcatc gagtgccgca taagtttttg cgttgacatt
     3721 ggcggcaaaa agtaagacta aacgatagtt gcttcaagca cgcttctcaa tcaacatttt
     3781 ttcaaatcaa atgggcacag gcaggtacca tccagtcccg cagaagtgct tatatatata
     3841 tatattatac actcacgtga gtgtgttcaa tgcaggtctg ttcctcaagt gcttagacca
     3901 aatttcggag cctctagtaa ttttgggagt tgggctggaa gatggggttg gtggggatcg
     3961 ggggagaggt gactggtgtg tggtcgagat ggtgaaggat tggatcccga tctctcacga
     4021 taactacaag caggtgcagg gaccgttcta ccacggaacc aaggcgaact tggcgatcgg
     4081 agatttgctg accaccggct tcatctccca cttcgaggac ggacgtatcc tgaagcacat
     4141 ctacttctcc gcgttgatgg agccggctgt gtggggagct gagctggcta tgtcgctgtc
     4201 tggcttggag ggacgtggct acatctacat cgtggagccg accggaccgt tcgaggacga
     4261 tccgaacctg accaacaaga agttcccggg caacccgacc cagtcctacc gcacctgcga
     4321 gccgttgcgc atcgtgggcg tggtggagga ctgggaggga cacccggtgg agttgatccg
     4381 tggaatgttg gactcgttgg aggacttgaa gcgccgtggc ttgcacgtca tcgaggatta
     4441 gtgatctcgg ccaggagtca cagaaaacaa gatcacttgg agatgtttca atcccgactt
     4501 gtgtcgtgcc agagtgctac ttgaaaactt gaaaattgcg gactgtcatg gattcgccct
     4561 tgtcttgtga tccttttttt gggggagcca ggtgagaaca atgttgtcga tgtgcttatt
     4621 tggcttcgca gtcaaaacat gggatacttg agacatgaaa gaaaaatgcc gcaacgatag
     4681 ctccatccaa ttccattcag ctccgactac agatgatagc gcttgacacc aatgacatgc
     4741 ttgtacagct gccatttgga aggcagggaa gctccataag ctcgggtccc caggactttg
     4801 gtcggtctca catcagattc ggctagccag cccatagcag ccgcgggaga tttcggttgt
     4861 ttgctacaat gattggggcg cctttctgcg aactttgtga catgtttcct caaaatgtca
     4921 agcaattttg atcttaaaag ttttgataat gcttgcttcc acaagcgacc tacagtagga
     4981 aatgtctcca caatctccac agattcagga ctcatcacta tgtgtgccgt gcaggggtag
     5041 ggcgcagaca tgacaacata caacacacat gaactaaaga atccaagtcg cggacaaaaa
     5101 aatctgatct tacacttaca cagaatgcag gttattagcg acgcttccat tgccaccgga
     5161 gtggcaatcg ttgaggcgct tcatcgaaca gagggtgaac ttcttgaggc tgggaggacc
     5221 gcgcagatgc ggctgataaa agtttcacaa gcacacggaa ttcactggcc gtcgttttac
     5281 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     5341 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     5401 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     5461 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     5521 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     5581 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     5641 catcaccgaa acgcgcga
//
