LOCUS       sequence-292687-        3248 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BglFusion compatible integration vector for Streptococcus 
            pneumoniae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Keller LE, Rueff AS, Kurushima J, Veening JW
  TITLE     Three New Integration Vectors and Fluorescent Proteins for Use in 
            the Opportunistic Human Pathogen Streptococcus pneumoniae.
  JOURNAL   Genes (Basel). 2019 May 22;10(5). pii: genes10050394. doi: 
            10.3390/genes10050394.
  PUBMED    31121970
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            (Basel)."; date: "2019-05-22"; volume: "10(5). pii: genes10050394. 
            doi"; pages: " 10.3390/genes10050394"
COMMENT     Sequence Label: pPEPY
FEATURES             Location/Qualifiers
     source          1..3248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      98..169
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      185..212
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1112..1700)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1192..1211)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2330..3124)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ggtctccgat cgtgtactgc agaaaactag tcgcaaaaaa ccccgcttcg gcggggtttt
       61 ttcgctctag acggtgatca acacgctagc caggcatcaa ataaaacgaa aggctcagtc
      121 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aatgctctct actagagtca
      181 cactggctca ccttcgggtg ggcctttctg cgtttataga attcatgaga tctaaataaa
      241 taaggaggaa aattaatgcc ggatccctcc agtaactcga gaaagtgtaa gcaattctgc
      301 agtactagga cgccgccaag ccagcttaaa cccagctcaa tgagctgggt tttttgtttg
      361 ttaaaaatga agaagaaact gtgaagcgta tttatagcaa agcactcaaa agtttacctt
      421 atgggtgctt ttttcgtgct tttttgaaaa gacaaaaaaa agaaccttgc caagcaagat
      481 tcttttgata gcgcagttga gcagtgtctt tagtaaattt agggacttgt tttgtcatag
      541 ttttgatacc tgtattcgtt gtttcatgag catttgacgt ttcttttcat gtaggcattt
      601 caacaattag aatgatattt ttccgaggat gaacttatat attgtgactt tttagagtta
      661 tgatgcctct tgatttgact atcgctatca atttcatcca cttttacagt ttcatggttt
      721 tatgaaccga aacatctagg tacaggtttt agcatcaatt gattgttttt gagccatatg
      781 aagttgaaac gtggagatac ttttcttgtg ataacgccct attcaaagag gaataaccgt
      841 ttttggagag agttcagtac tatgtggcgc taaatactat atgtaaaaca tatattaaac
      901 aagatttaac taaggagaat tgtaaaaaca aaaatgagaa tttcatataa agtttattct
      961 aggagtatgt atcttgaata aaaattgtta tttaagttct gggggttatg atgaatgttt
     1021 ttatgcggcc gcgggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggccg
     1081 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1141 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1201 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1261 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     1321 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1381 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1441 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     1501 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     1561 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     1621 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     1681 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtgg taccaaacct
     1741 tactaaagta tataatttag gcagtcaatt ctgtatgata atgtttgtac tatcgtcgag
     1801 tttttgcttg attgcctaac gagataaatt ttagaagagt tttcggtgtg atttgacaag
     1861 tttctgactg gaaggatagt tacactagta ttgtttatta aaatgagcag atagagaaaa
     1921 tttggaatct atttgcttat tatcaatcgt ttttttagct tatttgtctt agattggtat
     1981 agaactgagc tatggattaa tcttcttcta tcttatcctg ttcctaaata tgttggtaga
     2041 aaaatgaagc agttgttacg agtgtctgta ttggaaaaaa attttgtcta tacaattctt
     2101 taaatgatat gaagactagg aatgccaaaa cactgacata tttcgtttaa agcctatcca
     2161 gtctcttcaa ttgtatttgt agaaatgcta tcagtaatat attcgcgaac actatgattt
     2221 acaacagtgt ggtcgtacat ctttgtaact ttattataca gcttctagca aaaaactgga
     2281 cggagtcccg tccagaaaag taagcacttg ttattgcaat aaaattagcc taaaacaatt
     2341 catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa
     2401 aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg
     2461 caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt
     2521 tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct
     2581 cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt
     2641 cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt
     2701 cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa
     2761 agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat
     2821 actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat
     2881 gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt
     2941 ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat
     3001 ataggttttc attttctccc accagcttat ataccttagc aggagacatt ccttccgtat
     3061 cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag
     3121 ccattaattt tcctccttat ttatttagat ccttgcatgt atagttatta taccaggggg
     3181 acagtgcaat gtcaagctga aatcttcgag cagaagaacc ttgccaagca agattctttt
     3241 gatagggt
//
