LOCUS       sequence-292690-        5277 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses sfCFP under lac promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Alnahhas RN, Sadeghpour M, Chen Y, Frey AA, Ott W, Josic K, Bennett 
            MR
  TITLE     Majority sensing in synthetic microbial consortia.
  JOURNAL   Nat Commun. 2020 Jul 21;11(1):3659. doi: 10.1038/s41467-020-17475-z.
  PUBMED    32694598
REFERENCE   2  (bases 1 to 5277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-21"; volume: "11(1):3659. doi"; pages: " 
            10.1038/s41467-020-17475-z"
COMMENT     Sequence Label: C163
FEATURES             Location/Qualifiers
     source          1..5277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    252..268
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     257..279
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             470..499
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    634..650
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    665..681
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             766..1479
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1676..1747
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             1957..2148
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(2142..2164)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    2250..2389
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2305..2324
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2404..2421)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2575..3163)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2655..2674)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(3274..3293)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3285..4100
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSSMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3358..3377)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      complement(4763..4790)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 cagcgtcatg actgaacgct tagtcacact gcgagtaaca ccgtaagcca gccattgacc
       61 agccagtgcc ttagtgccca gcttgacttt ctcagagatt tcaccagtgt tctcatcggt
      121 cacggtaact acttcgttat cggtcccatt gattgcgtct gcttgtagaa tctcgttgac
      181 tttcttagca acaatcccgt agatgtcctg aacggtttca ctaggaagca agttaagacg
      241 tctgtgtgga attgtgagcg gataacaatt tcacacaggg ccctcggaca ccgaggagaa
      301 tgtcaagagg cgaacacaca acgtcttgga gcgccagagg aggaacgagc taaaacggag
      361 cttttttgcc ctgcgtgacc agatcccgga gttggaaaac aatgaaaagg cccccaaggt
      421 agttatcctt aaaaaagcca cagcatacat cctgtccgtc caagcagagg agcaaaagct
      481 catttctgaa gaggacttgt tgcggaaacg acgagaacag ttgaaacaca aacttgaaca
      541 gctacggaac tcttgtgcgt aaggaaaagt aaggaaaacg attccttcta acagaaatgt
      601 cctgagcaat cacctatgaa ctgtcgattg acattgtgag cggataacaa gatactgagc
      661 acaattgtga gcggataaca attgcatgcc acttaaaaag gagatcaaca atgaaagcaa
      721 ttttcgtact gaaacatctt aatcatgcta aggagaaata ctagtatgcg taaaggcgaa
      781 gagctgttca ctggtgtcgt ccctattctg gtggaactgg atggtgatgt caacggtcat
      841 aagttttccg tgcgtggcga gggtgaaggt gacgcaacta atggtaaact gacgctgaag
      901 ttcatctgta ctactggtaa actgccggta ccttggccga ctctggtaac gacgctgact
      961 tggggtgttc agtgctttgc tcgttatccg gaccatatga agcagcatga cttcttcaag
     1021 tccgccatgc cggaaggcta tgtgcaggaa cgcacgattt cctttaagga tgacggcacg
     1081 tacaaaacgc gtgcggaagt gaaatttgaa ggcgataccc tggtaaaccg cattgagctg
     1141 aaaggcattg actttaaaga agacggcaat atcctgggcc ataagctgga atacaattac
     1201 atcagcgaca atgtttacat caccgccgat aaacaaaaaa atggcattaa agcgaatttt
     1261 aaaattcgcc acaacgtgga ggatggcagc gtgcagctgg ctgatcacta ccagcaaaac
     1321 actccaatcg gtgatggtcc tgttctgctg ccagacaatc actatctgag cacgcaaagc
     1381 gttctgtcta aagatccgaa cgagaaacgc gatcatatgg ttctgctgga gttcgtaacc
     1441 gcagcgggca tcacgcatgg tatggatgaa ctgtacaaag cagcgaacga cgaaaattac
     1501 gcccttgcag cgtaataaga gctcgaacgc agaagcggtc tgataaaaca gaatttgcct
     1561 ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt
     1621 agcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat
     1681 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1741 cgctctctac tagagtcata agtgcggaag cgctggcatt gaccctgagt gatttttctc
     1801 tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag taaccgggca
     1861 tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg tatcattacc
     1921 cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga aaaaaccgcc
     1981 cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa actcaacgag
     2041 ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc tgatgagctt
     2101 taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     2161 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     2221 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
     2281 ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccatt
     2341 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
     2401 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     2461 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     2521 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     2581 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     2641 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     2701 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     2761 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     2821 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     2881 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2941 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     3001 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     3061 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     3121 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     3181 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgaa
     3241 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac
     3301 gggaaacgtc ttgctctagg ccgcgattaa attccagcat ggatgctgat ttatatgggt
     3361 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga
     3421 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
     3481 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc
     3541 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc gggaaaacag
     3601 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
     3661 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg
     3721 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt
     3781 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt
     3841 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt
     3901 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat
     3961 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac
     4021 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga
     4081 tgctcgatga gtttttctaa gaattaattc atgagcggat acatatttga atgtatttag
     4141 aaaaataaac aaataggggt tccgcgcaca tttccccggc tagccgcctg gtgctacgcc
     4201 tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc gacccggtcg
     4261 tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt accggtttat
     4321 tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag tttgctcagg
     4381 ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc cagagcctga
     4441 taaaaacggt tagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca
     4501 tcctgcgatg cagatccgga acataatggt gcagggcgct tgtttcggcg tgggtatggt
     4561 ggcaggcccc gtggccgggg gactgttggg cgctgccggc acctgtccta cgagttgcat
     4621 gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga
     4681 gctaccgcct gcggccgcta ggaaataata aaaaagccgg attaataatc tggcttttta
     4741 tattctctct ctagtatata aacgcagaaa ggcccacccg aaggtgagcc agtgtgagac
     4801 agcggtgcgg actgttgtaa ctcagaataa gaaatgaggc cgctcatggc gttgactctc
     4861 agtcatagta tcgtggtatc accggttggt tccactctct gttgcgggca acttcagcag
     4921 cacgtagggg aagcttttat tactcgagaa gcgcgtctga ataggcttct tgtattcctg
     4981 ccacacaggg aaaccatcag gagttaccca atgcacagcg caacgcttgc gaagaatctc
     5041 tccagtcttc ttatctttga cctcagcagc cagcagctta gcagcagact taagccagtt
     5101 cattgcttca accgcagcta ccaccgtcac gctcacagat tcccaaatca gcttagccat
     5161 gtatccagca gcctgattcg gctgagtgaa catcagaccc ttgccggaat caatagctgg
     5221 ctgaatggta tcttccagca cttgttgacg gaagccgaac tctttggacc cgtaagc
//
