LOCUS       sequence-292692-        5277 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses sfYFP under lac promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Alnahhas RN, Sadeghpour M, Chen Y, Frey AA, Ott W, Josic K, Bennett 
            MR
  TITLE     Majority sensing in synthetic microbial consortia.
  JOURNAL   Nat Commun. 2020 Jul 21;11(1):3659. doi: 10.1038/s41467-020-17475-z.
  PUBMED    32694598
REFERENCE   2  (bases 1 to 5277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-21"; volume: "11(1):3659. doi"; pages: " 
            10.1038/s41467-020-17475-z"
COMMENT     Sequence Label: C164
FEATURES             Location/Qualifiers
     source          1..5277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    161..177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     166..188
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             379..408
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    543..559
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    574..590
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             675..1388
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1585..1656
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             1866..2057
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(2051..2073)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    2159..2298
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2214..2233
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2313..2330)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2484..3072)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2564..2583)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(3183..3202)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3194..4009
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSSMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3267..3286)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      complement(4672..4699)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 tcagagattt caccagtgtt ctcatcggtc acggtaacta cttcgttatc ggtcccattg
       61 attgcgtctg cttgtagaat ctcgttgact ttcttagcaa caatcccgta gatgtcctga
      121 acggtttcac taggaagcaa gttaagacgt ctgtgtggaa ttgtgagcgg ataacaattt
      181 cacacagggc cctcggacac cgaggagaat gtcaagaggc gaacacacaa cgtcttggag
      241 cgccagagga ggaacgagct aaaacggagc ttttttgccc tgcgtgacca gatcccggag
      301 ttggaaaaca atgaaaaggc ccccaaggta gttatcctta aaaaagccac agcatacatc
      361 ctgtccgtcc aagcagagga gcaaaagctc atttctgaag aggacttgtt gcggaaacga
      421 cgagaacagt tgaaacacaa acttgaacag ctacggaact cttgtgcgta aggaaaagta
      481 aggaaaacga ttccttctaa cagaaatgtc ctgagcaatc acctatgaac tgtcgattga
      541 cattgtgagc ggataacaag atactgagca caattgtgag cggataacaa ttgcatgcca
      601 cttaaaaagg agatcaacaa tgaaagcaat tttcgtactg aaacatctta atcatgctaa
      661 ggagaaatac tagtatgcgt aaaggcgaag agctgttcac tggtgtcgtc cctattctgg
      721 tggaactgga tggtgatgtc aacggtcata agttttccgt gcgtggcgag ggtgaaggtg
      781 acgcaactaa tggtaaactg acgctgaagt tcatctgtac tactggtaaa ctgccggtac
      841 cttggccgac tctggtaacg acgctgactt atggtgttca gtgctttgct cgttatccgg
      901 accatatgaa gcagcatgac ttcttcaagt ccgccatgcc ggaaggctat gtgcaggaac
      961 gcacgatttc ctttaaggat gacggcacgt acaaaacgcg tgcggaagtg aaatttgaag
     1021 gcgataccct ggtaaaccgc attgagctga aaggcattga ctttaaagaa gacggcaata
     1081 tcctgggcca taagctggaa tacaatttta acagccacaa tgtttacatc accgccgata
     1141 aacaaaaaaa tggcattaaa gcgaatttta aaattcgcca caacgtggag gatggcagcg
     1201 tgcagctggc tgatcactac cagcaaaaca ctccaatcgg tgatggtcct gttctgctgc
     1261 cagacaatca ctatctgagc taccaaagcg ttctgtctaa agatccgaac gagaaacgcg
     1321 atcatatggt tctgctggag ttcgtaaccg cagcgggcat cacgcatggt atggatgaac
     1381 tgtacaaagc agcgaacgac gaaaattacg cccttgcagc gtaataagag ctcgaacgca
     1441 gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc
     1501 ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg
     1561 cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     1621 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcataa gtgcggaagc
     1681 gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc gccagttgtt
     1741 taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt atcgtgagca
     1801 tcctctctcg tttcatcggt atcattaccc ccatgaacag aaatccccct tacacggagg
     1861 catcagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga
     1921 cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca gacatctgtg
     1981 aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg
     2041 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     2101 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
     2161 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
     2221 agagcagatt gtactgagag tgcaccattg cggtgtgaaa taccgcacag atgcgtaagg
     2281 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     2341 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2401 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2461 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2521 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2581 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2641 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2701 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2761 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2821 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2881 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     2941 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3001 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3061 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3121 aactcacgtt aagggatttt ggtcatgaac aataaaactg tctgcttaca taaacagtaa
     3181 tacaaggggt gttatgagcc atattcaacg ggaaacgtct tgctctaggc cgcgattaaa
     3241 ttccagcatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     3301 aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     3361 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     3421 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     3481 actcaccact gcgatccccg ggaaaacagc attccaggta ttagaagaat atcctgattc
     3541 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     3601 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     3661 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     3721 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
     3781 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     3841 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     3901 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     3961 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaag aattaattca
     4021 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     4081 ttccccggct agccgcctgg tgctacgcct gaataagtga taataagcgg atgaatggca
     4141 gaaattcgaa agcaaattcg acccggtcgt cggttcaggg cagggtcgtt aaatagccgc
     4201 ttatgtctat tgctggttta ccggtttatt gactaccgga agcagtgtga ccgtgtgctt
     4261 ctcaaatgcc tgaggccagt ttgctcaggc tctccccgtg gaggtaataa ttgacgatat
     4321 gatcatttat tctgcctccc agagcctgat aaaaacggtt agcgcttcgt taatacagat
     4381 gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg
     4441 cagggcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
     4501 gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg
     4561 atagtcatgc cccgcgccca ccggaaggag ctaccgcctg cggccgctag gaaataataa
     4621 aaaagccgga ttaataatct ggctttttat attctctctc tagtatataa acgcagaaag
     4681 gcccacccga aggtgagcca gtgtgagaca gcggtgcgga ctgttgtaac tcagaataag
     4741 aaatgaggcc gctcatggcg ttgactctca gtcatagtat cgtggtatca ccggttggtt
     4801 ccactctctg ttgcgggcaa cttcagcagc acgtagggga agcttttatt actcgagaag
     4861 cgcgtctgaa taggcttctt gtattcctgc cacacaggga aaccatcagg agttacccaa
     4921 tgcacagcgc aacgcttgcg aagaatctct ccagtcttct tatctttgac ctcagcagcc
     4981 agcagcttag cagcagactt aagccagttc attgcttcaa ccgcagctac caccgtcacg
     5041 ctcacagatt cccaaatcag cttagccatg tatccagcag cctgattcgg ctgagtgaac
     5101 atcagaccct tgccggaatc aatagctggc tgaatggtat cttccagcac ttgttgacgg
     5161 aagccgaact ctttggaccc gtaagccagc gtcatgactg aacgcttagt cacactgcga
     5221 gtaacaccgt aagccagcca ttgaccagcc agtgccttag tgcccagctt gactttc
//
