LOCUS       sequence-292694-        9577 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mApple expression regulated by the A. thaliana UBQ10 promoter, 
            co-expressed nourseothricin acetyl transferase selectable marker 
            (nat).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9577)
  TITLE     Quantitative comparison of specialised leaf promoters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pN_UBQ10/mApple
FEATURES             Location/Qualifiers
     source          1..9577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             677..1387
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1090..1109
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(1390..1414)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1734..1758
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3089..3718
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4147..5220
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5286..5480
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(5716..5738)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5824..5964
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5879..5898
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5979..5996)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6150..6738)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6230..6249)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6984..7775)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    8296..8320
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(8413..8665)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8688..9260)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     promoter        complement(9302..9481)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 ctcccatatg gtctcgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca
       61 cacgagtcgt gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca
      121 attagccaaa aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta
      181 gctattgctt caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct
      241 tcttctataa aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc
      301 aaaatcttaa aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc
      361 cttattctct caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt
      421 tggtttagat tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat
      481 catcttaatt ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt
      541 tttgtcgaat aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag
      601 tttgtgcgat cgaatttgtc gattaatctg agtttttctg attaacaggc ggccgcacta
      661 gtgatatcac aagtttatgg tgagcaaggg cgaggagaat aacatggcca tcatcaagga
      721 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
      781 gggcgagggc gagggccgcc cctacgaggc ctttcagacc gctaagctga aggtgaccaa
      841 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
      901 ggtctacatt aagcacccag ccgacatccc cgactacttc aagctgtcct tccccgaggg
      961 cttcaggtgg gagcgcgtga tgaacttcga ggacggcggc attattcacg ttaaccagga
     1021 ctcctccctg caggacggcg tgttcatcta caaggtgaag ctgcgcggca ccaacttccc
     1081 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctccg aggagcggat
     1141 gtaccccgag gacggcgccc tgaagagcga gatcaagaag aggctgaagc tgaaggacgg
     1201 cggccactac gccgccgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     1261 cggcgcctac atcgtcgaca tcaagttgga catcgtgtcc cacaacgagg actacaccat
     1321 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
     1381 caagtaatcg ttcagctttc ttgtacaaag tggtgatatc ccgcggccat gctagagtcc
     1441 gcaaaaatca ccagtctctc tctacaaatc tatctctctc tatttttctc cagaataatg
     1501 tgtgagtagt tcccagataa gggaattagg gttcttatag ggtttcgctc atgtgttgag
     1561 catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc
     1621 taattcctaa aaccaaaatc cagtgacctg caggcatgcg acgtcgggcc caagcttagc
     1681 ttgagcttgg atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg
     1741 atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa
     1801 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc
     1861 cctcgggatc aaagtacttt aaagtacttt aaagtacttt aaagtacttt gatccaaccc
     1921 ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac
     1981 atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt
     2041 cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac
     2101 gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac
     2161 caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc
     2221 gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta
     2281 cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac
     2341 ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag
     2401 ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt
     2461 gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag
     2521 gcccgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc
     2581 cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg
     2641 catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc
     2701 aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc
     2761 gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt
     2821 ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc
     2881 ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc
     2941 tggcggcctg gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt
     3001 gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag
     3061 taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg
     3121 cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc
     3181 cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg
     3241 ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa
     3301 ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc
     3361 tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga
     3421 catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg
     3481 aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga
     3541 ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg
     3601 cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg
     3661 cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt
     3721 taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc
     3781 gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg
     3841 gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa
     3901 ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc
     3961 aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga
     4021 acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg
     4081 cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga
     4141 atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat
     4201 gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa
     4261 gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa
     4321 ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat
     4381 tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg
     4441 gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt
     4501 ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac
     4561 gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg
     4621 aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc
     4681 cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac
     4741 accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta
     4801 tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg
     4861 gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac
     4921 ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt
     4981 ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg
     5041 atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag
     5101 ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc
     5161 ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa
     5221 tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc
     5281 tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg
     5341 tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata
     5401 aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa
     5461 acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg
     5521 cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc
     5581 gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc
     5641 gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg
     5701 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     5761 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     5821 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg
     5881 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     5941 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6001 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     6061 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     6121 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6181 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     6241 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     6301 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     6361 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     6421 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     6481 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     6541 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     6601 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     6661 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     6721 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     6781 aacgaaaact cacgttaagg gattttggtc atgcatgata tatctcccaa tttgtgtagg
     6841 gcttattatg cacgcttaaa aataataaaa gcagacttga cctgatagtt tggctgtgag
     6901 caattatgtg cttagtgcat ctaacgcttg agttaagccg cgccgcgaag cggcgtcggc
     6961 ttgaacgaat ttctagctag acattatttg ccgactacct tggtgatctc gcctttcacg
     7021 tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc ttcttgtcca
     7081 agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag gcgctccatt
     7141 gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct gtaccaaatg
     7201 cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg cgagttccat
     7261 agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg atcaaagagt
     7321 tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt cagcaagata
     7381 gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc attgcgctgc
     7441 cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc
     7501 acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga agccgaagtt
     7561 tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac ggtcaccgta
     7621 accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga gccgtacaaa
     7681 tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac ctctgatagt
     7741 tgagtcgata cttcggcgat caccgcttcc cccatgatgt ttaactttgt tttagggcga
     7801 ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc cacggcgtaa
     7861 cgcgcttgct gcttggatgc ccgaggcata gactgtaccc caaaaaaaca gtcataacaa
     7921 gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag
     7981 ttgcgtgagc gcatacgcta cttgcattac agcttacgaa ccgaacaggc ttatgtccac
     8041 tgggttcgtg cccgaattga tcacaggcag caacgctctg tcatcgttac aatcaacatg
     8101 ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa
     8161 tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc
     8221 ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag
     8281 ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc ttagacaact
     8341 taataacaca ttgcggacgt ttttaatgta ctgaattaac gccgaattga attatcagct
     8401 tgcatgccgg tcgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg
     8461 cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa
     8521 aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat
     8581 tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac
     8641 tttattgcca aatgtttgaa cgatctgctt gactctaggg gtcatcatta ggggcagggc
     8701 atgctcatgt agagcgcctg ctcgccgtcc gaggcggtgc cgtcgtacag ggcggtgtcc
     8761 aggccgcaga gggtgaaccc catccgccgg tacgcgtgga tcgccggtgc gttgacgttg
     8821 gtgacctcca gccagaggtg cccggcaccc cgctcgcggg cgaactccgt cgcgagcccc
     8881 atcaacgcgc gcccgacccc gtgcccccgg tgctccgggg cgacctcgat gtcctcgacg
     8941 gtcagccggc ggttccagcc ggagtacgag acgaccacga agcccgccag gtcgccgtcg
     9001 tccccgtacg cgacgaacgt ccgagagtcc gggtcgccgt cctccccgtc gtccgattcg
     9061 tcgtccgatt cgtcgtcggg gaacaccttg gtcaggggcg ggtccaccgg cacctcccgc
     9121 agggtgaagc cgtccccggt ggcggtgacg cggaagacgg tgtcggtggt gaaggaccca
     9181 tccagtgcct cgatggcctc ggcgtccccc gggacactgg tgcggtaccg gtaagccgtg
     9241 tcgtcaagag tggtacccat ggtagatccc ctcgatcgag ttgagagtga atatgagact
     9301 ctaattggat accgagggga atttatggaa cgtcagtgga gcatttttga caagaaatat
     9361 ttgctagctg atagtgacct taggcgactt ttgaacgcgc aataatggtt tctgacgtat
     9421 gtgcttagct cattaaactc cagaaacccg cggctcagtg gctccttcaa cgttgcggtt
     9481 ctgtcagttc caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac
     9541 tcccttaatt ctccgctcat gattgataat tcgagct
//
