LOCUS       sequence-292695-        5296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses sfCFP under Rhl promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Alnahhas RN, Sadeghpour M, Chen Y, Frey AA, Ott W, Josic K, Bennett 
            MR
  TITLE     Majority sensing in synthetic microbial consortia.
  JOURNAL   Nat Commun. 2020 Jul 21;11(1):3659. doi: 10.1038/s41467-020-17475-z.
  PUBMED    32694598
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-21"; volume: "11(1):3659. doi"; pages: " 
            10.1038/s41467-020-17475-z"
COMMENT     Sequence Label: C308
FEATURES             Location/Qualifiers
     source          1..5296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    183..201
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             402..431
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    585..603
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    615..633
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             717..1430
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1627..1698
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             1908..2099
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(2093..2115)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    2201..2340
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2256..2275
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2355..2372)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2526..3114)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2606..2625)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(3225..3244)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3236..4051
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSSMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3309..3328)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      complement(4714..4741)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ccttagtgcc cagcttgact ttctcagaga tttcaccagt gttctcatcg gtcacggtaa
       61 ctacttcgtt atcggtccca ttgattgcgt ctgcttgtag aatctcgttg actttcttag
      121 caacaatccc gtagatgtcc tgaacggttt cactaggaag caagttaaga cgtctgtgtg
      181 gatccctatc agtgatagag attcacacag ggccctcgga caccgaggag aatgtcaaga
      241 ggcgaacaca caacgtcttg gagcgccaga ggaggaacga gctaaaacgg agcttttttg
      301 ccctgcgtga ccagatcccg gagttggaaa acaatgaaaa ggcccccaag gtagttatcc
      361 ttaaaaaagc cacagcatac atcctgtccg tccaagcaga ggagcaaaag ctcatttctg
      421 aagaggactt gttgcggaaa cgacgagaac agttgaaaca caaacttgaa cagctacgga
      481 actcttgtgc gtaaggaaaa gtaaggaaaa cgattccttc taacagaaat gtcctgagca
      541 atcacctatg aactgtcgat cctgtgaaat ctggcagttt tacttcccta tcagtgatag
      601 agataatgag cacatcccta tcagtgatag agatgcatgc cacttaaaaa ggagatcaac
      661 aatgaaagca attttcgtac tgaaacatct taatcatgct aaggagaaat actagtatgc
      721 gtaaaggcga agagctgttc actggtgtcg tccctattct ggtggaactg gatggtgatg
      781 tcaacggtca taagttttcc gtgcgtggcg agggtgaagg tgacgcaact aatggtaaac
      841 tgacgctgaa gttcatctgt actactggta aactgccggt accttggccg actctggtaa
      901 cgacgctgac ttggggtgtt cagtgctttg ctcgttatcc ggaccatatg aagcagcatg
      961 acttcttcaa gtccgccatg ccggaaggct atgtgcagga acgcacgatt tcctttaagg
     1021 atgacggcac gtacaaaacg cgtgcggaag tgaaatttga aggcgatacc ctggtaaacc
     1081 gcattgagct gaaaggcatt gactttaaag aagacggcaa tatcctgggc cataagctgg
     1141 aatacaatta catcagcgac aatgtttaca tcaccgccga taaacaaaaa aatggcatta
     1201 aagcgaattt taaaattcgc cacaacgtgg aggatggcag cgtgcagctg gctgatcact
     1261 accagcaaaa cactccaatc ggtgatggtc ctgttctgct gccagacaat cactatctga
     1321 gcacgcaaag cgttctgtct aaagatccga acgagaaacg cgatcatatg gttctgctgg
     1381 agttcgtaac cgcagcgggc atcacgcatg gtatggatga actgtacaaa gcagcgaacg
     1441 acgaaaatta cgcccttgca gcgtaataag agctcgaacg cagaagcggt ctgataaaac
     1501 agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag
     1561 tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc
     1621 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1681 ttgtcggtga acgctctcta ctagagtcat aagtgcggaa gcgctggcat tgaccctgag
     1741 tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca
     1801 gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg
     1861 gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg
     1921 aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga
     1981 aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg
     2041 ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
     2101 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     2161 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
     2221 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
     2281 agtgcaccat tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     2341 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     2401 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     2461 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2521 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2581 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2641 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     2701 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     2761 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     2821 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     2881 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     2941 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     3001 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3061 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3121 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3181 ttggtcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
     3241 ccatattcaa cgggaaacgt cttgctctag gccgcgatta aattccagca tggatgctga
     3301 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     3361 attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     3421 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
     3481 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     3541 cgggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     3601 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     3661 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     3721 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     3781 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     3841 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     3901 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     3961 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     4021 gtttcatttg atgctcgatg agtttttcta agaattaatt catgagcgga tacatatttg
     4081 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccgg ctagccgcct
     4141 ggtgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     4201 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
     4261 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     4321 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc
     4381 ccagagcctg ataaaaacgg ttagcgcttc gttaatacag atgtaggtgt tccacagggt
     4441 agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc ttgtttcggc
     4501 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgctgccgg cacctgtcct
     4561 acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc
     4621 caccggaagg agctaccgcc tgcggccgct aggaaataat aaaaaagccg gattaataat
     4681 ctggcttttt atattctctc tctagtatat aaacgcagaa aggcccaccc gaaggtgagc
     4741 cagtgtgaga cagcggtgcg gactgttgta actcagaata agaaatgagg ccgctcatgg
     4801 cgttgactct cagtcatagt atcgtggtat caccggttgg ttccactctc tgttgcgggc
     4861 aacttcagca gcacgtaggg gaagctttta ttactcgaga agcgcgtctg aataggcttc
     4921 ttgtattcct gccacacagg gaaaccatca ggagttaccc aatgcacagc gcaacgcttg
     4981 cgaagaatct ctccagtctt cttatctttg acctcagcag ccagcagctt agcagcagac
     5041 ttaagccagt tcattgcttc aaccgcagct accaccgtca cgctcacaga ttcccaaatc
     5101 agcttagcca tgtatccagc agcctgattc ggctgagtga acatcagacc cttgccggaa
     5161 tcaatagctg gctgaatggt atcttccagc acttgttgac ggaagccgaa ctctttggac
     5221 ccgtaagcca gcgtcatgac tgaacgctta gtcacactgc gagtaacacc gtaagccagc
     5281 cattgaccag ccagtg
//
