LOCUS       sequence-292697-        7175 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7175)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 7175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: ADAMTS5_pCSdest
FEATURES             Location/Qualifiers
     source          1..7175
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..978
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        1013..1031
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1013..1030
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    1085..1109
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4137..4161)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    4205..4339
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4229..4248)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4283..4302
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4452..4472)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4452..4470)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4491..4507)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4491..4507)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4504..4526)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4515..4531
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4539..4545,4546..4563,4564..4569))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4584..4605
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4722..4739)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4893..5481)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4973..4992)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5652..6443,6444..6512))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6275..6294
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6513..6617)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6643..7098)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6780..6801)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6992..7011
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gccaattcaa tatggcgtat atggactcat gccaattcaa tatggtggat ctggacctgt
       61 gccaattcaa tatggcgtat atggactcgt gccaattcaa tatggtggat ctggacccca
      121 gccaattcaa tatggcggac ttggcaccat gccaattcaa tatggcggac ctggcactgt
      181 gccaactggg gaggggtcta cttggcacgg tgccaagttt gaggaggggt cttggccctg
      241 tgccaagtcc gccatattga attggcatgg tgccaataat ggcggccata ttggctatat
      301 gccaggatca atatataggc aatatccaat atggccctat gccaatatgg ctattggcca
      361 ggttcaatac tatgtattgg ccctatgcca tatagtattc catatatggg ttttcctatt
      421 gacgtagata gcccctccca atgggcggtc ccatatacca tatatggggc ttcctaatac
      481 cgcccatagc cactccccca ttgacgtcaa tggtctctat atatggtctt tcctattgac
      541 gtcatatggg cggtcctatt gacgtatatg gcgcctcccc cattgacgtc aattacggta
      601 aatggcccgc ctggctcaat gcccattgac gtcaatagga ccacccacca ttgacgtcaa
      661 tgggatggct cattgcccat tcatatccgt tctcacgccc cctattgacg tcaatgacgg
      721 taaatggccc acttggcagt acatcaatat ctattaatag taacttggca agtacattac
      781 tattggaagt acgccagggt acattggcag tactcccatt gacgtcaatg gcggtaaatg
      841 gcccgcgatg gctgccaagt acatccccat tgacgtcaat ggggaggggc aatgacgcaa
      901 atgggcgttc cattgacgta aatgggcggt aggcgtgcct aatgggaggt ctatataagc
      961 aatgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct tgatttaggt
     1021 gacactatag aatacaagct acttgttctt tttgcaggat cccatcgatt cgaattcaag
     1081 gatcacaagt ttgtacaaaa aagcaggctt taaaggaacc aattcagtcg actggatccg
     1141 gtaccgaatt cgcggccgcg aattcgccct tatcccccgc cccgttaact tcatagcaaa
     1201 taacaaatac ccataaagtc ccagtcgcgc agcccctccc cgcgggcagc gcactatgct
     1261 gctcgggtgg gcgtccctgc tgctgtgcgc gttccgcctg cccctggccg cggtcggccc
     1321 cgccgcgaca cctgcccagg ataaagccgg gcagcctccg actgctgcag cagccgccca
     1381 gccccgccgg cggcaggggg aggaggtgca ggagcgagcc gagcctcccg gccacccgca
     1441 ccccctggcg cagcggcgca ggagcaaggg gctggtgcag aacatcgacc aactctactc
     1501 cggcggcggc aaggtgggct acctcgtcta cgcgggcggc cggaggttcc tcttggacct
     1561 ggagcgagat ggttcggtgg gcattgctgg cttcgtgccc gcaggaggcg ggacgagtgc
     1621 gccctggcgc caccggagcc actgcttcta tcggggcaca gtggacgcta gtccccgctc
     1681 tctggctgtc tttgacctct gtgggggtct cgacggcttc ttcgcggtca agcacgcgcg
     1741 ctacacccta aagccactgc tgcgcggacc ctgggcggag gaagaaaagg ggcgcgtgta
     1801 cggggatggg tccgcacgga tcctgcacgt ctacacccgc gagggcttca gcttcgaggc
     1861 cctgccgccg cgcgccagct gcgaaacccc cgcgtccaca ccggaggccc acgagcatgc
     1921 tccggcgcac agcaacccga gcggacgcgc agcactggcc tcgcagctct tggaccagtc
     1981 cgctctctcg cccgctgggg gctcaggacc gcagacgtgg tggcggcggc ggcgccgctc
     2041 catctcccgg gcccgccagg tggagctgct tctggtggct gacgcgtcca tggcgcggtt
     2101 gtatggccgg ggcctgcagc attacctgct gaccctggcc tccatcgcca ataggctgta
     2161 cagccatgct agcatcgaga accacatccg cctggccgtg gtgaaggtgg tggtgctagg
     2221 cgacaaggac aagagcctgg aagtgagcaa gaacgctgcc accacactca agaacttttg
     2281 caagtggcag caccaacaca accagctggg agatgaccat gaggagcact acgatgcagc
     2341 tatcctgttt actcgggagg atttatgtgg gcatcattca tgtgacaccc tgggaatggc
     2401 agacgttggg accatatgtt ctccagagcg cagctgtgct gtgattgaag acgatggcct
     2461 ccacgcagcc ttcactgtgg ctcacgaaat cggacattta cttggcctct cccatgacga
     2521 ttccaaattc tgtgaagaga cctttggttc cacagaagat aagcgcttaa tgtcttccat
     2581 ccttaccagc attgatgcat ctaagccctg gtccaaatgc acttcagcca ccatcacaga
     2641 attcctggat gatggccatg gtaactgttt gctggaccta ccacgaaagc agatcctggg
     2701 ccccgaagaa ctcccaggac agacctacga tgccacccag cagtgcaacc tgacattcgg
     2761 gcctgagtac tccgtgtgtc ccggcatgga tgtctgtgct cgcctgtggt gtgctgtggt
     2821 acgccagggc cagatggtct gtctgaccaa gaagctgcct gcggtggaag ggacgccttg
     2881 tggaaagggg agaatctgcc tgcagggcaa atgtgtggac aaaaccaaga aaaaatatta
     2941 ttcaacgtca agccatggca actggggatc ttggggatcc tggggccagt gttctcgctc
     3001 atgtggagga ggagtgcagt ttgcctatcg tcactgtaat aaccctgctc ccagaaacaa
     3061 cggacgctac tgcacaggga agagggccat ctaccgctcc tgcagtctca tgccctgccc
     3121 acccaatggt aaatcatttc gtcatgaaca gtgtgaggcc aaaaatggct atcagtctga
     3181 tgcaaaagga gtcaaaactt ttgtggaatg ggttcccaaa tatgcaggtg tcctgccagc
     3241 ggatgtgtgc aagctgacct gcagagccaa gggcactggc tactatgtgg tattttctcc
     3301 aaaggtgacc gatggcactg aatgtaggcc gtacagtaat tccgtctgcg tccgggggaa
     3361 gtgtgtgaga actggctgtg acggcatcat tggctcaaag ctgcagtatg acaagtgcgg
     3421 agtatgtgga ggagacaact ccagctgtac aaagattgtt ggaaccttta ataagaaaag
     3481 taagggttac actgacgtgg tgaggattcc tgaaggggca acccacataa aagttcgaca
     3541 gttcaaagcc aaagaccaga ctagattcac tgcctattta gccctgaaaa agaaaaacgg
     3601 tgagtacctt atcaatggaa agtacatgat ctccacttca gagactatca ttgacatcaa
     3661 tggaacagtc atgaactata gcggttggag ccacagggat gacttcctgc atggcatggg
     3721 ctactctgcc acgaaggaaa ttctaatagt gcagattctt gcaacagacc ccactaaacc
     3781 attagatgtc cgttatagct tttttgttcc caagaagtcc actccaaaag taaactctgt
     3841 cactagtcat ggcagcaata aagtgggatc acacacttcg cagccgcagt gggtcacggg
     3901 cccatggctc gcctgctcta ggacctgtga cacaggttgg cacaccagaa cggtgcagtg
     3961 ccaggatgga aaccggaagt tagcaaaagg atgtcctctc tcccaaaggc cttctgcgtt
     4021 taagcaatgc ttgttgaaga aatgttagcc tgtggttatg atcttatgca caaagataac
     4081 tggaggattc agcaccgatg cagtcgtgta tataaagggc gaattcgttt atctagaccc
     4141 agctttcttg tacaaagtgg tgatcctctc gagcctctag aactatagtg agtcgtatta
     4201 cgtagatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     4261 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     4321 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     4381 ggaggtgtgg gaggtttttt aattcgcggc cgcggcgcca atgcattggg cccggtaccc
     4441 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
     4501 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cgggagcata
     4561 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     4621 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     4681 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     4741 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     4801 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     4861 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     4921 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     4981 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     5041 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     5101 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     5161 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     5221 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     5281 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     5341 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     5401 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     5461 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     5521 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     5581 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     5641 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     5701 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     5761 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     5821 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     5881 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     5941 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     6001 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     6061 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     6121 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     6181 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     6241 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     6301 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     6361 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     6421 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     6481 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     6541 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     6601 caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata
     6661 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     6721 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     6781 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     6841 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     6901 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     6961 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     7021 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     7081 cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     7141 cggtgcgggc ctcttcgcta ttacgccagt cgacc
//
