LOCUS       sequence-292698-        3819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Individual component of Caffeic Acid biosynthesis pathway, hpaC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-03-25"; volume: "9. pii: 52786. doi"; pages: " 
            10.7554/eLife.52786"
COMMENT     Sequence Label: pCO706
FEATURES             Location/Qualifiers
     source          1..3819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1617..1635)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1703..1807
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2029..2820
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2991..3579
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3480..3499
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3733..3750
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcaaat gaaacccgag gacttcaggg cctcaacaca aagacccttt acaggtgaag
       61 aataccttaa gtctctccag gacggacgag agatatatat atacggggaa agggtcaaag
      121 acgttacaac ccatcccgcc ttcagaaatg ctgccgcctc tgtggctcag ctctatgatg
      181 ccttgcataa accggaaatg caagactcat tatgttggaa tacggatact ggctctggcg
      241 gttacaccca taaattcttt agagtcgcaa agtcagctga cgatttacgt cagcagcgag
      301 atgcaattgc cgagtggtcc aggttgtctt atggttggat ggggcgtacc cctgactata
      361 aggccgcctt tggatgtgcc ttgggtgcaa acccagggtt ttatggccag ttcgagcaga
      421 atgcccgaaa ctggtacacc aggatccaag aaaccgggct ctacttcaac cacgcaatcg
      481 ttaatcctcc gatcgacaga cacctcccta cggacaaggt gaaagatgtt tatataaagt
      541 tggagaaaga gacagatgca ggaattatcg ttagcggagc taaggtcgtg gccactaact
      601 ccgctctcac acactataat atgatcggat ttggatctgc tcaagtcatg ggagaaaatc
      661 ctgactttgc tcttatgttt gttgctccaa tggatgctga tggcgttaaa ttgatctcac
      721 gagcttccta cgaaatggta gctggggcca cgggtagtcc ctacgactat cccttgtcct
      781 cccgatttga tgaaaatgat gccatcttgg tcatggacaa tgtccttatt ccatgggaaa
      841 acgttttgat ctaccgagat ttcgaccgat gtaggcgatg gaccatggag ggcggcttcg
      901 ccagaatgta ccctttacaa gcatgcgtcc gtcttgccgt taaactcgac tttataaccg
      961 ccttgttgaa aaagtcatta gaatgcaccg gcaccctcga atttcgagga gtccaagctg
     1021 acctggggga ggttgtggca tggcgaaaca cattttgggc cctcagtgac tcaatgtgca
     1081 gcgaggccac tccgtgggta aacggcgcct atctgccgga ccatgccgca ttgcaaacgt
     1141 accgtgtgct cgcacctatg gcctatgcta agataaagaa cattatagaa cgtaatgtta
     1201 caagcggact gatctatctc ccaagctccg ccagggatct caacaatccc caaatagacc
     1261 aatatcttgc aaaatacgta aggggatcaa acgggatgga tcacgttcaa aggattaaaa
     1321 tcttaaagct catgtgggac gcaatcgggt ctgagttcgg tgggagacac gagctttacg
     1381 agattaacta tagcggttct caggatgaga taaggcttca gtgtctgaga caggcacaaa
     1441 acagtggcaa tatggataaa atgatggcaa tggtcgatag gtgtttgagt gagtacgacc
     1501 aggatggatg gacggtcccc catctccaca ataatgatga cattaatatg ttagataaat
     1561 tgctgaagtc cggctgagct ttgagaccac gaagtggctc ttcagtggac gaaagggcct
     1621 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     1681 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     1741 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     1801 gaagagtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     1861 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ttggggtaca gtctatgcct
     1921 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
     1981 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gagggaagcg
     2041 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc
     2101 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca
     2161 cacagcgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga
     2221 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc
     2281 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag
     2341 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca
     2401 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc
     2461 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag
     2521 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga
     2581 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg
     2641 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata
     2701 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat
     2761 cagttggaag aatttgtcca ttacgtaaaa ggcgagatca ccaaggtagt cggcaaataa
     2821 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     2881 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     2941 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3001 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3061 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3121 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3181 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3241 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3301 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3361 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3421 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3481 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3541 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3601 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     3661 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     3721 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     3781 cctctccccg cgcgttggcc gattcattaa tcactctgt
//
