LOCUS       sequence-292700-        2769 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Individual component of Caffeic Acid biosynthesis pathway, hpaC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-03-25"; volume: "9. pii: 52786. doi"; pages: " 
            10.7554/eLife.52786"
COMMENT     Sequence Label: pCO707
FEATURES             Location/Qualifiers
     source          1..2769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(567..585)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        653..757
                     /gene="bla"
                     /label=AmpR promoter
     CDS             979..1770
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1941..2529
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2430..2449
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2683..2700
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcaaat gcaattagac gagcagcgtc ttagatttcg tgatgctatg gcttctcttt
       61 ctgccgctgt taatatcatt acaacggaag gcgacgcagg tcaatgcggc atcactgcca
      121 ctgctgtctg cagcgtcacg gacacgccac cgtctttgat ggtttgcata aatgccaatt
      181 cagcaatgaa ccctgtgttc caaggtaacg gcaagctctg tgtaaatgtt ctcaatcacg
      241 agcaagagtt aatggcacgt cactttgcag ggatgaccgg gatggcaatg gaggaacgtt
      301 tttccctctc ttgctggcaa aaaggcccgc tggctcaacc tgtactgaaa ggatcactcg
      361 cctcccttga gggggaaatc agagatgtgc aggctattgg cacacacctc gtgtacctcg
      421 ttgagattaa gaacataatt ttaagcgcag agggccacgg gctgatctac tttaagaggc
      481 gatttcatcc agttatgctg gaaatggagg ctgcaatcag tggctgagct ttgagaccac
      541 gaagtggctc ttcagtggac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
      601 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
      661 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
      721 ctgataaatg cttcaataat attgaaaaag gaagagtatg cgctcacgca actggtccag
      781 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
      841 ctgttttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc
      901 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct
      961 aaaacaaagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca
     1021 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
     1081 ggctccgcag tggatggcgg cctgaagcca cacagcgata ttgatttgct ggttacggtg
     1141 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
     1201 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
     1261 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
     1321 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
     1381 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
     1441 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
     1501 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     1561 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     1621 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
     1681 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ttacgtaaaa
     1741 ggcgagatca ccaaggtagt cggcaaataa ctgtcagacc aagtttactc atatatactt
     1801 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     1861 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1921 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1981 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2041 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     2101 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2161 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     2221 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2281 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     2341 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2401 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2461 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2521 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     2581 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     2641 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     2701 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     2761 tcactctgt
//
