LOCUS       sequence-292701-        3852 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Individual component of Caffeic Acid biosynthesis pathway, hpaC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-03-25"; volume: "9. pii: 52786. doi"; pages: " 
            10.7554/eLife.52786"
COMMENT     Sequence Label: pCO708
FEATURES             Location/Qualifiers
     source          1..3852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1650..1668)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1736..1840
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2062..2853
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3024..3612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3513..3532
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3766..3783
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcaaat gacgctccaa tcacagactg ctaaagactg ccttgcatta gatggcgctt
       61 taacgctggt gcaatgcgag gccatcgcta cacataggtc ccgaataagc gtcactcctg
      121 ccctcagaga gagatgtgca agggctcacg cccgtctgga acacgccatc gccgagcaaa
      181 gacatatata cggcataacc actgggtttg gcccgcttgc aaatcgactg attggagcag
      241 atcaaggcgc agaactccaa caaaacctta tttaccacct tgccacgggg gttgggccaa
      301 aattgagttg ggctgaggca agggccctga tgttggccag gctgaactca attctccagg
      361 gagcttcagg ggcttcccca gagacaatcg atcgtatcgt agcagtactc aacgctggat
      421 ttgcccccga ggttccggct caaggaactg tcggcgcaag cggtgacctg accccgctcg
      481 cacacatggt gctcgctctg cagggccgag gtcgtatgat tgacccaagt ggccgtgttc
      541 aagaggccgg agccgtcatg gatcgtctgt gcggtgggcc cctgaccctg gctgccaggg
      601 acggactggc actggtaaac ggaacttccg ctatgacggc cattgccgcc ctcaccggtg
      661 tagaagctgc cagagccata gatgctgctt taaggcactc cgctgtactt atggaagtac
      721 ttagcggaca cgcagaggca tggcatcctg cctttgcaga gcttcgtcct catccaggtc
      781 aattgagagc cacagagcgt cttgcacaag ccttagatgg cgccggtaga gtgtgtagga
      841 ccttaactgc tgcaagacgt cttaccgccg ccgacctgag gcccgaggac catcccgcac
      901 aggatgccta ctcattgaga gtagttccgc aattagtagg ggcagtctgg gacacacttg
      961 attggcatga cagggtagtc acgtgcgagt taaacagcgt cacagataac cctatatttc
     1021 ctgagggctg cgctgtgcct gctctccatg gcgggaactt tatgggtgta catgtcgcac
     1081 tcgcttctga tgcactgaac gctgcattgg taactctggc tggtttagtt gagaggcaga
     1141 tcgcaagact gacagacgag aagttgaata agggcttacc agcattcctc cacggaggac
     1201 aggctggatt acaaagtggg ttcatggggg cccaagtcac cgctactgct ttgctcgccg
     1261 aaatgcgagc taacgctacc cctgtctcag tacagtctct ttcaacaaat ggcgcaaacc
     1321 aggacgttgt gtccatgggt acgattgcag ccaggcgagc acgagcacaa ttgttaccgc
     1381 tgtcacagat tcaggctatt ttggcattag ctttggcaca agctatggat ttgttagacg
     1441 atccggaagg acaagcaggc tggtcactca ctgccaggga cctcagggac cgaatcaggg
     1501 ctgtaagtcc cggattacgt gccgataggc cacttgccgg gcacattgag gcagtcgcac
     1561 aaggacttcg tcatccatct gccgctgccg accctccggc tagtggttga gctttgagac
     1621 cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     1681 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     1741 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     1801 accctgataa atgcttcaat aatattgaaa aaggaagagt atgcgctcac gcaactggtc
     1861 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     1921 tgactgtttt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     1981 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
     2041 cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta
     2101 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     2161 tacggctccg cagtggatgg cggcctgaag ccacacagcg atattgattt gctggttacg
     2221 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     2281 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     2341 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     2401 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     2461 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     2521 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     2581 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     2641 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     2701 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     2761 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccattacgta
     2821 aaaggcgaga tcaccaaggt agtcggcaaa taactgtcag accaagttta ctcatatata
     2881 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     2941 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     3001 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3061 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3121 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     3181 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3241 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3301 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3361 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3421 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3481 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3541 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3601 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3661 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     3721 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     3781 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     3841 taatcactct gt
//
