LOCUS       sequence-29311-e         642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-MAPK1
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..106
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      107..116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcgggcccaa taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat
       61 gagcaatgct tttttataat gccaactttg tacaaaaaag ttggcaccat ggcggcggcg
      121 gcggcgggcg cgggcccgga gatggtccgc gggcaggtgt tcgacgtggg gccgcgctac
      181 accaacctct cgtacatcgg cgagggcgcc tacggcatgg tgtgctctgc ttatgataat
      241 gtcaacaaag ttcgagtagc tatcaagaaa atcagcccct ttgagcacca gacctactgc
      301 cagagaaccc tgagggagat aaaaatctta ctgcgcttca gacatgagaa catcattgga
      361 atcaatgaca ttattcgagc accaaccatc gagcaaatga aagatgtata tatagtacag
      421 gacctcatgg aaacagatct ttacaagctc ttgaagacac aacacctcag caatgaccat
      481 atctgctatt ttctctacca gatcctcaga gggttaaaat atatccattc agctaacgtt
      541 ctgcaccgtg acctcaagcc ttccaacctg ctgctcaaca ccacctgtga tctcaagatc
      601 tgtgactttg gcctggcccg tgttgcagat ccagaccatg at
//
