LOCUS       sequence-29315-e         603 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-BRAF
FEATURES             Location/Qualifiers
     source          1..603
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    9..108
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      109..118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctcgggccc aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg
       61 atgagcaatg cttttttata atgccaactt tgtacaaaaa agttggcacc atggcggcgc
      121 tgagcggtgg cggtggtggc ggcgcggagc cgggccaggc tctgttcaac ggggacatgg
      181 agcccgaggc cggcgccggc gccggcgccg cggcctcttc ggctgcggac cctgccattc
      241 cggaggaggt gtggaatatc aaacaaatga ttaagttgac acaggaacat atagaggccc
      301 tattggacaa atttggtggg gagcataatc caccatcaat atatctggag gcctatgaag
      361 aatacaccag caagctagat gcactccaac aaagagaaca acagttattg gaatctctgg
      421 ggaacggaac tgatttttct gtttctagct ctgcatcaat ggataccgtt acatcttctt
      481 cctcttctag cctttcagtg ctaccttcat ctctttcagt ttttcaaaat cccacagatg
      541 tggcacggag caaccccaag tcaccacaaa aacctatcgt tagagtcttc ctgcccaaca
      601 aac
//
